P50932 (FMT_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	RP209

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) + H2O = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: EC methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Methionyl-tRNA formyltransferase HAMAP MF_00182		PRO_0000083033
Regions
	Region	108 – 111	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50932 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 2238362E782566ED	FASTA	303	34,159
	10 20 30 40 50 60 MKVIFMGTPE FAVPTLKKLI IHHEVKAVFT QQPKAKGRGL HLAKSPIHQL AFEHQIPVYS 70 80 90 100 110 120 PSTLRNDETI NLIKKIDADI IVVIAYGFIV PKAILEAKKY GCLNIHPSDL PRHRGAAPLQ 130 140 150 160 170 180 RTIIEGDRKS SVCIMRMDSG LDTGDILLKE DLNLERRITL DELSNKCAHL GAELLIKTLA 190 200 210 220 230 240 NIDNIVPIKQ SSNGITYAHK LTKAEGKINW YESAYSIDCK IRGMNPWPGA YFSYNDKIIK 250 260 270 280 290 300 ILRAEYFNYN HHFIPGTVIN NKLEIACGSG ILRVKKLQQE SKKALNIEEF LRGTNILKDT ILK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
[2]	"Unusual organization of the rRNA genes in Rickettsia prowazekii." Andersson S.G.E., Zomorodipour A., Winkler H.H., Kurland C.G. J. Bacteriol. 177:4171-4175(1995) [PubMed: 7608097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-303. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ235270 Genomic DNA. Translation: CAA14674.1. Z49077 Genomic DNA. Translation: CAA88897.1.
PIR	C71732.
RefSeq	NP_220597.1. NC_000963.1.
3D structure databases
ProteinModelPortal	P50932.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883930.
GenomeReviews	Gene locus RP209 in contig AJ235269_GR.
KEGG	rpr:RP209.
PATRIC	17901176. VBIRicPro72556_0218.
Phylogenomic databases
HOGENOM	HBG571560.
OMA	AEGKINW.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	RPRO272947:RP209-MONOMER.
Family and domain databases
HAMAP	MF_00182. Formyl_trans. [Tree]
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
Gene3D	G3DSA:3.10.25.10. Formyl_trans_C. 1 hit. G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K00604.
PANTHER	PTHR11138. Met_tRNA_Form_TA-like. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. Fmt. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_RICPR

Accession

Primary (citable) accession number: P50932

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program