P50921 (TPIS_VIBMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Synonyms:	tim, tpi

Organism

Vibrio marinus (Moritella marina)

Taxonomic identifier

90736 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Moritellaceae › Moritella

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer. Ref.1
Subcellular location	Cytoplasm Probable HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 256

256

Triosephosphate isomerase HAMAP MF_00147_B

PRO_0000090314

Sites

Active site

97

1

Electrophile

Active site

169

1

Proton acceptor

Binding site

9

1

Substrate By similarity

Binding site

11

1

Substrate

Secondary structure

1

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256

Helix Strand Turn

Details...

Beta strand

5 – 9

5

Helix

16 – 29

14

Turn

30 – 32

3

Beta strand

35 – 41

7

Helix

44 – 46

3

Helix

47 – 57

11

Beta strand

62 – 66

5

Beta strand

72 – 75

4

Helix

82 – 85

4

Helix

86 – 88

3

Beta strand

92 – 96

5

Helix

98 – 103

6

Helix

108 – 120

13

Beta strand

124 – 129

6

Helix

133 – 137

5

Helix

141 – 156

16

Helix

158 – 161

4

Beta strand

165 – 168

4

Helix

171 – 173

3

Turn

174 – 176

3

Helix

182 – 197

16

Helix

201 – 206

6

Beta strand

208 – 211

4

Turn

217 – 219

3

Helix

220 – 224

5

Beta strand

231 – 235

5

Helix

236 – 239

4

Helix

241 – 254

14

Sequences

	Sequence		Length	Mass (Da)
	P50921 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 5CC4BB8CFF27070A	FASTA	256	26,740
	10 20 30 40 50 60 MRHPVVMGNW KLNGSKEMVV DLLNGLNAEL EGVTGVDVAV APPALFVDLA ERTLTEAGSA 70 80 90 100 110 120 IILGAQNTDL NNSGAFTGDM SPAMLKEFGA THIIIGHSER REYHAESDEF VAKKFAFLKE 130 140 150 160 170 180 NGLTPVLCIG ESDAQNEAGE TMAVCARQLD AVINTQGVEA LEGAIIAYEP IWAIGTGKAA 190 200 210 220 230 240 TAEDAQRIHA QIRAHIAEKS EAVAKNVVIQ YGGSVKPENA AAYFAQPDID GALVGGAALD 250 AKSFAAIAKA AAEAKA

References

[1]

"Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties." Alvarez M., Zeelen J.P., Mainfroid V., Rentier-Delrue F., Martial J.A., Wyns L., Wierenga R.K., Maes D. J. Biol. Chem. 273:2199-2206(1998) [PubMed: 9442062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG, SUBUNIT. Strain: ATCC 15382.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U48935 Genomic DNA. Translation: AAA88910.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AW1	X-ray	2.70	A/B/D/E/G/H/J/K	1-256	[»]
1AW2	X-ray	2.65	A/B/D/E/G/H/J/K	1-256	[»]

ProteinModelPortal

P50921.

SMR

P50921. Positions 2-240.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

HAMAP

MF_00147_B. TIM_B.
[Tree]

InterPro

IPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

PANTHER

PTHR21139. Triophos_ismrse. 1 hit.

Pfam

PF00121. TIM. 1 hit.
[Graphical view]

SUPFAM

SSF51351. Triophos_ismrse. 1 hit.

TIGRFAMs

TIGR00419. Tim. 1 hit.

PROSITE

PS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TPIS_VIBMA

Accession

Primary (citable) accession number: P50921

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 31, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program