Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Moritella marina (Vibrio marinus)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).UniRule annotation1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation1 Publication

Kineticsi

Kcat is 420000 min(-1) for isomerase activity with D-glyceraldehyde 3-phosphate as substrate (at pH 7.6 and 10 degrees Celsius).1 Publication
  1. KM=1.9 mM for D-glyceraldehyde 3-phosphate (at pH 7.6 and 10 degrees Celsius)1 Publication

    Temperature dependencei

    Optimum temperature is 15 degrees Celsius.1 Publication

    Pathwayi: gluconeogenesis

    This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
    View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

    Pathwayi: glycolysis

    This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. Triosephosphate isomerase (tpiA)
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei97ElectrophileUniRule annotation1 Publication1
    Active sitei169Proton acceptorUniRule annotation1
    Binding sitei175Substrate; via amide nitrogenUniRule annotation1 Publication1
    Binding sitei214SubstrateUniRule annotation1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase
    Biological processGluconeogenesis, Glycolysis

    Enzyme and pathway databases

    BRENDAi5.3.1.1 6633
    UniPathwayiUPA00109; UER00189
    UPA00138

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Triosephosphate isomerase1 PublicationUniRule annotation (EC:5.3.1.1UniRule annotation1 Publication)
    Short name:
    TIM1 PublicationUniRule annotation
    Short name:
    TPI1 PublicationUniRule annotation
    Alternative name(s):
    Triose-phosphate isomerase1 PublicationUniRule annotation
    Gene namesi
    Name:tpiA1 PublicationUniRule annotation
    Synonyms:tim, tpi
    OrganismiMoritella marina (Vibrio marinus)
    Taxonomic identifieri90736 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesMoritellaceaeMoritella

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi238A → S: Reduces catalytic efficiency and affinity, but increases thermal stability. 1 Publication1

    Chemistry databases

    DrugBankiDB02726 2-Phosphoglycolic Acid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000903141 – 256Triosephosphate isomeraseAdd BLAST256

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation1 Publication

    Structurei

    Secondary structure

    1256
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi5 – 9Combined sources5
    Helixi16 – 29Combined sources14
    Turni30 – 32Combined sources3
    Beta strandi35 – 41Combined sources7
    Helixi44 – 46Combined sources3
    Helixi47 – 57Combined sources11
    Beta strandi62 – 66Combined sources5
    Beta strandi72 – 75Combined sources4
    Helixi82 – 87Combined sources6
    Beta strandi92 – 96Combined sources5
    Helixi98 – 103Combined sources6
    Helixi108 – 120Combined sources13
    Beta strandi124 – 129Combined sources6
    Helixi133 – 137Combined sources5
    Helixi141 – 156Combined sources16
    Helixi158 – 161Combined sources4
    Beta strandi165 – 168Combined sources4
    Turni171 – 175Combined sources5
    Helixi182 – 197Combined sources16
    Helixi201 – 206Combined sources6
    Beta strandi208 – 211Combined sources4
    Turni217 – 219Combined sources3
    Helixi220 – 223Combined sources4
    Beta strandi231 – 235Combined sources5
    Helixi236 – 239Combined sources4
    Helixi241 – 254Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1AW1X-ray2.70A/B/D/E/G/H/J/K1-256[»]
    1AW2X-ray2.65A/B/D/E/G/H/J/K1-256[»]
    ProteinModelPortaliP50921
    SMRiP50921
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP50921

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni9 – 11Substrate bindingUniRule annotation1 Publication3
    Regioni235 – 236Substrate bindingUniRule annotation1 Publication2

    Sequence similaritiesi

    Belongs to the triosephosphate isomerase family.UniRule annotation

    Family and domain databases

    CDDicd00311 TIM, 1 hit
    Gene3Di3.20.20.70, 1 hit
    HAMAPiMF_00147_B TIM_B, 1 hit
    InterProiView protein in InterPro
    IPR013785 Aldolase_TIM
    IPR035990 TIM_sf
    IPR022896 TrioseP_Isoase_bac/euk
    IPR000652 Triosephosphate_isomerase
    IPR020861 Triosephosphate_isomerase_AS
    PANTHERiPTHR21139 PTHR21139, 1 hit
    PfamiView protein in Pfam
    PF00121 TIM, 1 hit
    SUPFAMiSSF51351 SSF51351, 1 hit
    TIGRFAMsiTIGR00419 tim, 1 hit
    PROSITEiView protein in PROSITE
    PS00171 TIM_1, 1 hit
    PS51440 TIM_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P50921-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRHPVVMGNW KLNGSKEMVV DLLNGLNAEL EGVTGVDVAV APPALFVDLA
    60 70 80 90 100
    ERTLTEAGSA IILGAQNTDL NNSGAFTGDM SPAMLKEFGA THIIIGHSER
    110 120 130 140 150
    REYHAESDEF VAKKFAFLKE NGLTPVLCIG ESDAQNEAGE TMAVCARQLD
    160 170 180 190 200
    AVINTQGVEA LEGAIIAYEP IWAIGTGKAA TAEDAQRIHA QIRAHIAEKS
    210 220 230 240 250
    EAVAKNVVIQ YGGSVKPENA AAYFAQPDID GALVGGAALD AKSFAAIAKA

    AAEAKA
    Length:256
    Mass (Da):26,740
    Last modified:October 1, 1996 - v1
    Checksum:i5CC4BB8CFF27070A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U48935 Genomic DNA Translation: AAA88910.1

    Similar proteinsi

    Entry informationi

    Entry nameiTPIS_MORMI
    AccessioniPrimary (citable) accession number: P50921
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: April 25, 2018
    This is version 100 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health