P50914 (RL14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L14

Alternative name(s):
CAG-ISL 7

Gene names

Name:

RPL14

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Polymorphism	The poly-Ala stretch is highly polymorphic.
Sequence similarities	Belongs to the ribosomal protein L14e family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism Triplet repeat expansion
Domain	Repeat
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Traceable author statement. Source: Reactome nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Traceable author statement. Source: Reactome rRNA processing Inferred from mutant phenotype PubMed 18697920. Source: UniProtKB ribosomal large subunit biogenesis Inferred from mutant phenotype PubMed 18697920. Source: UniProtKB translational elongation Traceable author statement. Source: Reactome translational initiation Traceable author statement. Source: Reactome translational termination Traceable author statement. Source: Reactome viral transcription Traceable author statement. Source: Reactome
Cellular_component	cytosolic large ribosomal subunit Inferred from direct assay Ref.9. Source: UniProtKB
Molecular_function	RNA binding Traceable author statement Ref.2. Source: ProtInc structural constituent of ribosome Non-traceable author statement Ref.9. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PHLDA1	Q8WV24	2	EBI-356746,EBI-738731

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.9
	Chain	2 – 215	214	60S ribosomal protein L14		PRO_0000132031
Regions
	Repeat	171 – 175	5	1-1
	Repeat	176 – 180	5	1-2
	Repeat	181 – 185	5	1-3
	Repeat	186 – 190	5	1-4
	Repeat	193 – 195	3	2-1
	Repeat	196 – 198	3	2-2
	Region	171 – 190	20	4 X 5 AA tandem repeats of Q-K-A-[PAS]-X
	Region	193 – 198	6	2 X 3 AA tandem repeats of K-[GA]-Q
Amino acid modifications
	Modified residue	79	1	N6-acetyllysine Ref.17
	Modified residue	85	1	N6-acetyllysine Ref.17
	Modified residue	139	1	Phosphoserine Ref.11 Ref.12 Ref.13 Ref.15 Ref.16 Ref.18 Ref.20
Natural variations
	Natural variant	138	1	A → S.		VAR_013633
	Natural variant	158 – 159	2	Missing.		VAR_047109
	Natural variant	159	1	A → AA. Ref.2 Ref.3 Ref.5		VAR_013634
	Natural variant	159	1	A → AAA. Ref.2 Ref.3 Ref.5		VAR_014070
	Natural variant	159	1	A → AAAA. Ref.2 Ref.3 Ref.5		VAR_013635
	Natural variant	159	1	A → AAAAA. Ref.2 Ref.3 Ref.5		VAR_013636
	Natural variant	159	1	A → AAAAAA. Ref.2 Ref.3 Ref.5		VAR_006923
	Natural variant	159	1	A → AAAAAAAA. Ref.2 Ref.3 Ref.5		VAR_013637
Experimental info
	Sequence conflict	65	1	P → L in BAA13443. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P50914 [UniParc]. Last modified November 4, 2008. Version 4. Checksum: 4C63DE4FC8E687FE	FASTA	215	23,432
	10 20 30 40 50 60 MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF 70 80 90 100 110 120 ILKFPHSAHQ KYVRQAWQKA DINTKWAATR WAKKIEARER KAKMTDFDRF KVMKAKKMRN 130 140 150 160 170 180 RIIKNEVKKL QKAALLKASP KKAPGTKGTA AAAAAAAAAK VPAKKITAAS KKAPAQKVPA 190 200 210 QKATGQKAAP APKAQKGQKA PAQKAPAPKA SGKKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of trinucleotide repeat-containing genes in human pancreatic islets." Aoki M., Koranyi L., Riggs A.C., Wasson J., Chiu K.C., Vaxillaire M., Froguel P., Gough S., Liu L., Donis-Keller H., Permutt M.A. Diabetes 45:157-164(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT 158-ALA-ALA-159 DEL. Tissue: Pancreas.
[2]	"Triplet repeat-containing ribosomal protein L14 gene in immortalized human endothelial cell line (t-HUE4)." Tanaka M., Tanaka T., Harata M., Suzuki T., Mitsui Y. Biochem. Biophys. Res. Commun. 243:531-537(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ALA-ALA-ALA-ALA-ALA-159 INS. Tissue: Umbilical vein.
[3]	"The human ribosomal protein genes: sequencing and comparative analysis of 73 genes." Yoshihama M., Uechi T., Asakawa S., Kawasaki K., Kato S., Higa S., Maeda N., Minoshima S., Tanaka T., Shimizu N., Kenmochi N. Genome Res. 12:379-390(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-ALA-ALA-ALA-ALA-159 INS.
[4]	Lin L., Nong W., Zhou G., Ke R., Shen C., Zhong G., Zheng Z., Liang M., Wen S., Li H., Yang S. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-ALA-ALA-ALA-ALA-159 INS.
[6]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], POLYMORPHISM OF POLY-ALA REGION. Tissue: Brain, Kidney, Muscle, Ovary and Skin.
[8]	"A complete map of the human ribosomal protein genes: assignment of 80 genes to the cytogenetic map and implications for human disorders." Uechi T., Tanaka T., Kenmochi N. Genomics 72:223-230(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 85-116.
[9]	"Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing." Odintsova T.I., Muller E.C., Ivanov A.V., Egorov T.A., Bienert R., Vladimirov S.N., Kostka S., Otto A., Wittmann-Liebold B., Karpova G.G. J. Protein Chem. 22:249-258(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-10, MASS SPECTROMETRY.
[10]	Bienvenut W.V. Submitted (AUG-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 12-46; 80-85; 104-109; 148-158 AND 188-193, VARIANT 158-ALA-ALA-159 DEL, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[15]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, MASS SPECTROMETRY.
[16]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[17]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-79 AND LYS-85, MASS SPECTROMETRY.
[18]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[19]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[20]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U16738 mRNA. Translation: AAC16021.1. D87735 mRNA. Translation: BAA13443.1. AB061822 Genomic DNA. Translation: BAB79460.1. DQ118667 mRNA. Translation: AAZ38460.1. AK223111 mRNA. Translation: BAD96831.1. AC104186 Genomic DNA. No translation available. BC000606 mRNA. Translation: AAH00606.1. BC005134 mRNA. Translation: AAH05134.1. BC009294 mRNA. Translation: AAH09294.1. BC019651 mRNA. Translation: AAH19651.1. BC022805 mRNA. Translation: AAH22805.1. BC029036 mRNA. Translation: AAH29036.1. AB046407 Genomic DNA. Translation: BAB21253.1.
IPI	IPI00555744.
RefSeq	NP_001030168.1. NM_001034996.2. NP_003964.3. NM_003973.4.
UniGene	Hs.740386.
3D structure databases
ProteinModelPortal	P50914.
ModBase	Search...
Protein-protein interaction databases
IntAct	P50914. 17 interactions.
MINT	MINT-1156434.
PTM databases
PhosphoSite	P50914.
Polymorphism databases
DMDM	212276521.
2D gel databases
SWISS-2DPAGE	P50914.
Proteomic databases
PaxDb	P50914.
PeptideAtlas	P50914.
PRIDE	P50914.
Protocols and materials databases
DNASU	9045.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000338970; ENSP00000345156; ENSG00000188846. ENST00000396203; ENSP00000379506; ENSG00000188846.
GeneID	9045.
KEGG	hsa:9045.
UCSC	uc003ckg.3. human.
Organism-specific databases
CTD	9045.
GeneCards	GC03P040501.
H-InvDB	HIX0003196. HIX0036844.
HGNC	HGNC:10305. RPL14.
HPA	HPA004136.
neXtProt	NX_P50914.
PharmGKB	PA34673.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2163.
HOVERGEN	HBG005187.
InParanoid	P50914.
KO	K02875.
OMA	SWAKKIE.
OrthoDB	EOG4Z0B71.
Enzyme and pathway databases
Reactome	REACT_116125. Disease. REACT_17015. Metabolism of proteins. REACT_1762. 3' -UTR-mediated translational regulation. REACT_21257. Metabolism of RNA. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P50914.
Bgee	P50914.
CleanEx	HS_RPL14.
Genevestigator	P50914.
GermOnline	ENSG00000188846. Homo sapiens.
Family and domain databases
Gene3D	2.30.30.30. 1 hit.
InterPro	IPR014722. Rib_L2_dom2. IPR002784. Ribosomal_L14. [Graphical view]
Pfam	PF01929. Ribosomal_L14e. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RpL14. human.
GenomeRNAi	9045.
NextBio	33879.

Entry information

Entry name

RL14_HUMAN

Accession

Primary (citable) accession number: P50914
Secondary accession number(s): Q45RF0 , Q53G20, Q8TBD5, Q8WUT0, Q92579, Q96GR0, Q9BSB8, Q9BW65, Q9BYF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 4, 2008
Last modified:	May 1, 2013
This is version 135 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.