P50908 (TRPA_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase alpha chain
EC=4.2.1.20

Gene names

Name:	trpA
Ordered Locus Names:	TM_0137

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. HAMAP MF_00131
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP MF_00131
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP MF_00131
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpA family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	tryptophan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Tryptophan synthase alpha chain HAMAP MF_00131		PRO_0000098864
Sites
	Active site	34	1	Proton acceptor By similarity
	Active site	45	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	189	1	F → L in CAA63392. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50908 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: F6B06FBCA83294FA	FASTA	239	26,759
	10 20 30 40 50 60 MKGFIAYIPA GFPDLETTRK ILIALNELGI TGVEIGVPFS DPVADGPVIQ LAHSVALRNG 70 80 90 100 110 120 VTIKKILEML SEISVDYDLY LMSYLNPIVN YPEGKEKLLD ELKKLGVKGL IIPDLPLREV 130 140 150 160 170 180 KNVDIAYPIV PFVAPNTKDE EIDLINSVQA PFVYYISRYG VTGEREDLPF ADHIKRVKER 190 200 210 220 230 IKLPLFVGFG ISRHEQVKKV WEIADGVIVG SALVRIMEEN PKDEIPRKVV EKVKELLGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"(Beta alpha)8-barrel proteins of tryptophan biosynthesis in the hyperthermophile Thermotoga maritima." Sterner R., Dahm A., Darimont B., Ivens A., Liebl W., Kirschner K. EMBO J. 14:4395-4402(1995) [PubMed: 7556082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92729 Genomic DNA. Translation: CAA63392.1. AE000512 Genomic DNA. Translation: AAD35230.1.
PIR	S59050.
RefSeq	NP_227952.1. NC_000853.1.
3D structure databases
ProteinModelPortal	P50908.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	896967.
GenomeReviews	Gene locus TM_0137 in contig AE000512_GR.
KEGG	tma:TM0137.
NMPDR	fig|243274.1.peg.136.
PATRIC	23935116. VBITheMar51294_0136.
TIGR	TM_0137.
Phylogenomic databases
HOGENOM	HBG391690.
OMA	NPIYIYG.
PhylomeDB	P50908.
ProtClustDB	PRK13111.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-344. TMAR243274:TM_0137-MONOMER.
Family and domain databases
HAMAP	MF_00131. Trp_synth_alpha. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR011060. RibuloseP-bd_barrel. IPR018204. Trp_synthase_alpha_AS. IPR002028. Trp_synthase_suA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01695.
Pfam	PF00290. Trp_syntA. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00262. TrpA. 1 hit.
PROSITE	PS00167. TRP_SYNTHASE_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPA_THEMA

Accession

Primary (citable) accession number: P50908

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program