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Protein

Pancreatic triacylglycerol lipase

Gene

PNLIP

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei169 – 1691Nucleophile
Active sitei193 – 1931Charge relay system
Metal bindingi204 – 2041Calcium; via carbonyl oxygenBy similarity
Metal bindingi207 – 2071Calcium; via carbonyl oxygenBy similarity
Metal bindingi209 – 2091CalciumBy similarity
Metal bindingi212 – 2121CalciumBy similarity
Active sitei280 – 2801Charge relay system

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

ESTHERicavpo-1plip. Pancreatic_lipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic triacylglycerol lipase (EC:3.1.1.3)
Short name:
PL
Short name:
PTL
Short name:
Pancreatic lipase
Gene namesi
Name:PNLIP
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence analysisAdd
BLAST
Chaini17 – 465449Pancreatic triacylglycerol lipasePRO_0000017783Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi20 ↔ 26PROSITE-ProRule annotation
Disulfide bondi107 ↔ 118PROSITE-ProRule annotation
Disulfide bondi254 ↔ 278PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi316 ↔ 321PROSITE-ProRule annotation
Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence analysis
Glycosylationi398 – 3981N-linked (GlcNAc...)Sequence analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence analysis
Disulfide bondi449 ↔ 465PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Inductioni

By colipase/CLPS in the presence of bile salts.

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000012083.

Structurei

3D structure databases

ProteinModelPortaliP50903.
SMRiP50903. Positions 18-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini355 – 465111PLATPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHRX. Eukaryota.
ENOG410Y92X. LUCA.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiP50903.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR013818. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50903-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLWILSLF LETVAGGEVC FDRLGCFSDN KPWAGTSERP RKGLPWDPSA
60 70 80 90 100
INVRFLLYTN ENPNNYQRIT ADSSVIRSSD FKTDRKTRFI IHGFIDKGEE
110 120 130 140 150
NWLADLCKAL FQVESVNCIC VDWRGGSRTL YSQASQNIQV VGAEVAYLIN
160 170 180 190 200
FLQSQLDYPP SSVHIIGHSL GSHAAGEAGR RTNGAIGRIT GLDPAEPYFQ
210 220 230 240 250
YTPEIVRLDP SDAQFVDVIH TDGNPIIPNL GFGMSQTVGH LDFFPNGGLQ
260 270 280 290 300
MPGCQKNILS QIVDIDGIWE GTRDFAACNH LRSYKYYIDS ITNPKGFAGF
310 320 330 340 350
SCDSYSSFSS NKCFPCATGE CPQMGHYADK FPGKTKENFQ NFYLNTGDKS
360 370 380 390 400
NFSRWRYRIA VTLSGQKVTG HVLVSLFGDA GNTKQYEIYR GSLKPGNNHS
410 420 430 440 450
NEIDSDVDVG DLQKVKFIWY NNVINITLPK VGASRITVTR SDGRVFDFCS
460
QDTVREEVLL TLQPC
Length:465
Mass (Da):51,858
Last modified:October 1, 1996 - v1
Checksum:iCF3A26CBB1A6C708
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77403 mRNA. Translation: CAA54585.1.
PIRiS41084.
RefSeqiNP_001166472.1. NM_001173001.1.

Genome annotation databases

GeneIDi100135601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77403 mRNA. Translation: CAA54585.1.
PIRiS41084.
RefSeqiNP_001166472.1. NM_001173001.1.

3D structure databases

ProteinModelPortaliP50903.
SMRiP50903. Positions 18-465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000012083.

Protein family/group databases

ESTHERicavpo-1plip. Pancreatic_lipase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100135601.

Organism-specific databases

CTDi5406.

Phylogenomic databases

eggNOGiENOG410IHRX. Eukaryota.
ENOG410Y92X. LUCA.
HOGENOMiHOG000038552.
HOVERGENiHBG003243.
InParanoidiP50903.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR016272. Lipase_LIPH.
IPR013818. Lipase_N.
IPR002331. Lipase_panc.
IPR001024. PLAT/LH2_dom.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTSiPR00823. PANCLIPASE.
PR00821. TAGLIPASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF49723. SSF49723. 1 hit.
SSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of the classical guinea pig pancreatic lipase and comparison with the lipase related protein 2."
    Carriere F., Thirstrup K., Hjorth S., Boel E.
    FEBS Lett. 338:63-68(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.

Entry informationi

Entry nameiLIPP_CAVPO
AccessioniPrimary (citable) accession number: P50903
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 16, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.