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P50900

- GUX2_CLOSH

UniProt

P50900 - GUX2_CLOSH

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Protein

Exoglucanase-2

Gene

celY

Organism
Clostridium stercorarium subsp. stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

GO - Molecular functioni

  1. cellulase activity Source: InterPro
  2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
  3. cellulose binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiCBM3. Carbohydrate-Binding Module Family 3.
GH48. Glycoside Hydrolase Family 48.

Names & Taxonomyi

Protein namesi
Recommended name:
Exoglucanase-2 (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase II
Avicelase II
Exocellobiohydrolase II
Exoglucanase II
Gene namesi
Name:celY
Ordered Locus Names:Cst_c18970
OrganismiClostridium stercorarium subsp. stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754)
Taxonomic identifieri1121335 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000011220: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 914881Exoglucanase-2PRO_0000008030Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini763 – 914152CBM3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CBM3 (carbohydrate binding type-3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 4 hits.
2.60.40.10. 1 hit.
2.60.40.710. 1 hit.
4.10.870.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR005102. Carbo-bd_X2.
IPR001956. CBD_3.
IPR027390. Endoglucanase_F_dom3.
IPR000556. Glyco_hydro_48F.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF00942. CBM_3. 1 hit.
PF03442. CBM_X2. 1 hit.
PF02011. Glyco_hydro_48. 1 hit.
[Graphical view]
PRINTSiPR00844. GLHYDRLASE48.
SMARTiSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS51172. CBM3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50900-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRRLMKGIS LLTLVFLIGI MLQLSLKSEL TAYASSDDPY KQRFLELWEE
60 70 80 90 100
LHDPSNGYFS SHGIPYHAVE TLIVEAPDYG HLTTSEAMSY YLWLEALYGK
110 120 130 140 150
FTGDFSYFMK AWETIEKYMI PTEQDQPNRS MAGYNPAKPA TYAPEWEEPS
160 170 180 190 200
MYPSQLDFSA PVGIDPIYNE LVSTYGTNTI YGMHWLLDVD NWYGFGRRAD
210 220 230 240 250
RISSPAYINT FQRGSQESVW ETIPQPCWDD LTIGGRNGFL DLFVGDSQYS
260 270 280 290 300
AQFKYTNAPD ADARAIQATY WANQWAKEHG VNLSQYVKKA SRMGDYLRYA
310 320 330 340 350
MFDKYFRKIG DSKQAGTGYD AAHYLLSWYY AWGGGITADW AWIIGCSHVH
360 370 380 390 400
AGYQNPMTAW ILANDPEFKP ESPNGANDWA KSLERQLEFY QWLQSAEGAI
410 420 430 440 450
AGGATNSYKG RYETLPAGIS TFYGMAYEEH PVYLDPGSNT WFGFQAWTMQ
460 470 480 490 500
RVAEYYYLTG DTRAEQLLDK WVDWIKSVVR LNSDGTFEIP GNLEWSGQPD
510 520 530 540 550
TWTGTYTGNP NLHVSVVSYG TDLGAAGSLA NALLYYAKTS GDDEARNLAK
560 570 580 590 600
ELLDRMWNLY RDDKGLSAPE TREDYVRFFE QEVYVPQGWS GTMPNGDRIE
610 620 630 640 650
PGVTFLDIRS KYLNDPDYPK LQQAYNEGKA PVFNYHRFWA QCDIAIANGL
660 670 680 690 700
YSILFGSEQA NDSFITPTSA TFDKNNQEDI SVTVTYNGNT LLGIKSGSSY
710 720 730 740 750
LIEGVDYIVN GDVIIIKKEF LAGQATGSIS LLFDFSAGLD RTLTIDIIDT
760 770 780 790 800
GGGEEPVEPV EPVEGVLIIQ SFNANTQEIS NSIMPRFRIY NSGNTSIPLS
810 820 830 840 850
EVKLRYYYTV DGDKPQNFWC DWASIGSSNV TGTFVKMDGA TTGADYYLEI
860 870 880 890 900
GFTPQAGTLE PGASIEVQGR FSKIDWTDYT QTNDYSFNPT ASSYVDFNKI
910
TAYISGNLVY GIEP
Length:914
Mass (Da):102,921
Last modified:June 26, 2013 - v2
Checksum:iB24CFC80F3AF4C7C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti520 – 5201G → R in CAA93280. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69359 Genomic DNA. Translation: CAA93280.1.
CP004044 Genomic DNA. Translation: AGC68874.1.
RefSeqiWP_015359554.1. NC_020134.1.
YP_007373484.1. NC_020134.1.

Genome annotation databases

EnsemblBacteriaiAGC68874; AGC68874; Cst_c18970.
GeneIDi14472210.
KEGGicss:Cst_c18970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z69359 Genomic DNA. Translation: CAA93280.1 .
CP004044 Genomic DNA. Translation: AGC68874.1 .
RefSeqi WP_015359554.1. NC_020134.1.
YP_007373484.1. NC_020134.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM3. Carbohydrate-Binding Module Family 3.
GH48. Glycoside Hydrolase Family 48.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AGC68874 ; AGC68874 ; Cst_c18970 .
GeneIDi 14472210.
KEGGi css:Cst_c18970.

Family and domain databases

Gene3Di 1.50.10.10. 4 hits.
2.60.40.10. 1 hit.
2.60.40.710. 1 hit.
4.10.870.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR005102. Carbo-bd_X2.
IPR001956. CBD_3.
IPR027390. Endoglucanase_F_dom3.
IPR000556. Glyco_hydro_48F.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
Pfami PF00942. CBM_3. 1 hit.
PF03442. CBM_X2. 1 hit.
PF02011. Glyco_hydro_48. 1 hit.
[Graphical view ]
PRINTSi PR00844. GLHYDRLASE48.
SMARTi SM01067. CBM_3. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS51172. CBM3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Bronnenmeier K., Kundt K., Riedel K., Schwarz W.H., Staudenbauer W.L.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 35414 / DSM 8532 / NCIMB 11754.
  2. "Complete genome sequence of Clostridium stercorarium subsp. stercorarium strain DSM 8532, a thermophilic degrader of plant cell wall fibers."
    Poehlein A., Zverlov V.V., Daniel R., Schwarz W.H., Liebl W.
    Genome Announc. 1:E0007313-E0007313(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35414 / DSM 8532 / NCIMB 11754.
  3. "Purification and properties of a novel type of exo-1,4-beta-glucanase (avicelase II) from the cellulolytic thermophile Clostridium stercorarium."
    Bronnenmeier K., Ruecknagel K.P., Staudenbauer W.L.
    Eur. J. Biochem. 200:379-385(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: ATCC 35414 / DSM 8532 / NCIMB 11754.

Entry informationi

Entry nameiGUX2_CLOSH
AccessioniPrimary (citable) accession number: P50900
Secondary accession number(s): L7VQ95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 26, 2013
Last modified: November 26, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3