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P50900 (GUX2_CLOSH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exoglucanase-2

EC=3.2.1.91
Alternative name(s):
1,4-beta-cellobiohydrolase II
Avicelase II
Exocellobiohydrolase II
Exoglucanase II
Gene names
Name:celY
Ordered Locus Names:Cst_c18970
OrganismClostridium stercorarium subsp. stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754) [Complete proteome] [HAMAP]
Taxonomic identifier1121335 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeunclassified Ruminococcaceae

Protein attributes

Sequence length914 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sequence similarities

Belongs to the glycosyl hydrolase 48 (cellulase L) family.

Contains 1 CBM3 (carbohydrate binding type-3) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 914881Exoglucanase-2
PRO_0000008030

Regions

Domain763 – 914152CBM3

Experimental info

Sequence conflict5201G → R in CAA93280. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P50900 [UniParc].

Last modified June 26, 2013. Version 2.
Checksum: B24CFC80F3AF4C7C

FASTA914102,921
        10         20         30         40         50         60 
MKRRLMKGIS LLTLVFLIGI MLQLSLKSEL TAYASSDDPY KQRFLELWEE LHDPSNGYFS 

        70         80         90        100        110        120 
SHGIPYHAVE TLIVEAPDYG HLTTSEAMSY YLWLEALYGK FTGDFSYFMK AWETIEKYMI 

       130        140        150        160        170        180 
PTEQDQPNRS MAGYNPAKPA TYAPEWEEPS MYPSQLDFSA PVGIDPIYNE LVSTYGTNTI 

       190        200        210        220        230        240 
YGMHWLLDVD NWYGFGRRAD RISSPAYINT FQRGSQESVW ETIPQPCWDD LTIGGRNGFL 

       250        260        270        280        290        300 
DLFVGDSQYS AQFKYTNAPD ADARAIQATY WANQWAKEHG VNLSQYVKKA SRMGDYLRYA 

       310        320        330        340        350        360 
MFDKYFRKIG DSKQAGTGYD AAHYLLSWYY AWGGGITADW AWIIGCSHVH AGYQNPMTAW 

       370        380        390        400        410        420 
ILANDPEFKP ESPNGANDWA KSLERQLEFY QWLQSAEGAI AGGATNSYKG RYETLPAGIS 

       430        440        450        460        470        480 
TFYGMAYEEH PVYLDPGSNT WFGFQAWTMQ RVAEYYYLTG DTRAEQLLDK WVDWIKSVVR 

       490        500        510        520        530        540 
LNSDGTFEIP GNLEWSGQPD TWTGTYTGNP NLHVSVVSYG TDLGAAGSLA NALLYYAKTS 

       550        560        570        580        590        600 
GDDEARNLAK ELLDRMWNLY RDDKGLSAPE TREDYVRFFE QEVYVPQGWS GTMPNGDRIE 

       610        620        630        640        650        660 
PGVTFLDIRS KYLNDPDYPK LQQAYNEGKA PVFNYHRFWA QCDIAIANGL YSILFGSEQA 

       670        680        690        700        710        720 
NDSFITPTSA TFDKNNQEDI SVTVTYNGNT LLGIKSGSSY LIEGVDYIVN GDVIIIKKEF 

       730        740        750        760        770        780 
LAGQATGSIS LLFDFSAGLD RTLTIDIIDT GGGEEPVEPV EPVEGVLIIQ SFNANTQEIS 

       790        800        810        820        830        840 
NSIMPRFRIY NSGNTSIPLS EVKLRYYYTV DGDKPQNFWC DWASIGSSNV TGTFVKMDGA 

       850        860        870        880        890        900 
TTGADYYLEI GFTPQAGTLE PGASIEVQGR FSKIDWTDYT QTNDYSFNPT ASSYVDFNKI 

       910 
TAYISGNLVY GIEP 

« Hide

References

« Hide 'large scale' references
[1]Bronnenmeier K., Kundt K., Riedel K., Schwarz W.H., Staudenbauer W.L.
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 35414 / DSM 8532 / NCIMB 11754.
[2]"Complete genome sequence of Clostridium stercorarium subsp. stercorarium strain DSM 8532, a thermophilic degrader of plant cell wall fibers."
Poehlein A., Zverlov V.V., Daniel R., Schwarz W.H., Liebl W.
Genome Announc. 1:E0007313-E0007313(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35414 / DSM 8532 / NCIMB 11754.
[3]"Purification and properties of a novel type of exo-1,4-beta-glucanase (avicelase II) from the cellulolytic thermophile Clostridium stercorarium."
Bronnenmeier K., Ruecknagel K.P., Staudenbauer W.L.
Eur. J. Biochem. 200:379-385(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: ATCC 35414 / DSM 8532 / NCIMB 11754.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z69359 Genomic DNA. Translation: CAA93280.1.
CP004044 Genomic DNA. Translation: AGC68874.1.
RefSeqYP_007373484.1. NC_020134.1.

3D structure databases

ProteinModelPortalP50900.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM3. Carbohydrate-Binding Module Family 3.
GH48. Glycoside Hydrolase Family 48.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID14472210.
KEGGcss:Cst_c18970.

Organism-specific databases

CMRSearch...

Family and domain databases

Gene3D1.50.10.10. 4 hits.
2.60.40.10. 1 hit.
2.60.40.710. 1 hit.
4.10.870.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR005102. Carbo-bd_X2.
IPR001956. CBD_3.
IPR027390. Endoglucanase_F_dom3.
IPR000556. Glyco_hydro_48F.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PfamPF00942. CBM_3. 1 hit.
PF03442. CBM_X2. 1 hit.
PF02011. Glyco_hydro_48. 1 hit.
[Graphical view]
PRINTSPR00844. GLHYDRLASE48.
SMARTSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEPS51172. CBM3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUX2_CLOSH
AccessionPrimary (citable) accession number: P50900
Secondary accession number(s): L7VQ95
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 26, 2013
Last modified: February 19, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries