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P50900

- GUX2_CLOSH

UniProt

P50900 - GUX2_CLOSH

Protein

Exoglucanase-2

Gene

celY

Organism
Clostridium stercorarium subsp. stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (26 Jun 2013)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

    GO - Molecular functioni

    1. cellulase activity Source: InterPro
    2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
    3. cellulose binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiCBM3. Carbohydrate-Binding Module Family 3.
    GH48. Glycoside Hydrolase Family 48.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exoglucanase-2 (EC:3.2.1.91)
    Alternative name(s):
    1,4-beta-cellobiohydrolase II
    Avicelase II
    Exocellobiohydrolase II
    Exoglucanase II
    Gene namesi
    Name:celY
    Ordered Locus Names:Cst_c18970
    OrganismiClostridium stercorarium subsp. stercorarium (strain ATCC 35414 / DSM 8532 / NCIMB 11754)
    Taxonomic identifieri1121335 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000011220: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 914881Exoglucanase-2PRO_0000008030Add
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini763 – 914152CBM3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CBM3 (carbohydrate binding type-3) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.50.10.10. 4 hits.
    2.60.40.10. 1 hit.
    2.60.40.710. 1 hit.
    4.10.870.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR005102. Carbo-bd_X2.
    IPR001956. CBD_3.
    IPR027390. Endoglucanase_F_dom3.
    IPR000556. Glyco_hydro_48F.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PfamiPF00942. CBM_3. 1 hit.
    PF03442. CBM_X2. 1 hit.
    PF02011. Glyco_hydro_48. 1 hit.
    [Graphical view]
    PRINTSiPR00844. GLHYDRLASE48.
    SMARTiSM01067. CBM_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49384. SSF49384. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS51172. CBM3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50900-1 [UniParc]FASTAAdd to Basket

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    MKRRLMKGIS LLTLVFLIGI MLQLSLKSEL TAYASSDDPY KQRFLELWEE    50
    LHDPSNGYFS SHGIPYHAVE TLIVEAPDYG HLTTSEAMSY YLWLEALYGK 100
    FTGDFSYFMK AWETIEKYMI PTEQDQPNRS MAGYNPAKPA TYAPEWEEPS 150
    MYPSQLDFSA PVGIDPIYNE LVSTYGTNTI YGMHWLLDVD NWYGFGRRAD 200
    RISSPAYINT FQRGSQESVW ETIPQPCWDD LTIGGRNGFL DLFVGDSQYS 250
    AQFKYTNAPD ADARAIQATY WANQWAKEHG VNLSQYVKKA SRMGDYLRYA 300
    MFDKYFRKIG DSKQAGTGYD AAHYLLSWYY AWGGGITADW AWIIGCSHVH 350
    AGYQNPMTAW ILANDPEFKP ESPNGANDWA KSLERQLEFY QWLQSAEGAI 400
    AGGATNSYKG RYETLPAGIS TFYGMAYEEH PVYLDPGSNT WFGFQAWTMQ 450
    RVAEYYYLTG DTRAEQLLDK WVDWIKSVVR LNSDGTFEIP GNLEWSGQPD 500
    TWTGTYTGNP NLHVSVVSYG TDLGAAGSLA NALLYYAKTS GDDEARNLAK 550
    ELLDRMWNLY RDDKGLSAPE TREDYVRFFE QEVYVPQGWS GTMPNGDRIE 600
    PGVTFLDIRS KYLNDPDYPK LQQAYNEGKA PVFNYHRFWA QCDIAIANGL 650
    YSILFGSEQA NDSFITPTSA TFDKNNQEDI SVTVTYNGNT LLGIKSGSSY 700
    LIEGVDYIVN GDVIIIKKEF LAGQATGSIS LLFDFSAGLD RTLTIDIIDT 750
    GGGEEPVEPV EPVEGVLIIQ SFNANTQEIS NSIMPRFRIY NSGNTSIPLS 800
    EVKLRYYYTV DGDKPQNFWC DWASIGSSNV TGTFVKMDGA TTGADYYLEI 850
    GFTPQAGTLE PGASIEVQGR FSKIDWTDYT QTNDYSFNPT ASSYVDFNKI 900
    TAYISGNLVY GIEP 914
    Length:914
    Mass (Da):102,921
    Last modified:June 26, 2013 - v2
    Checksum:iB24CFC80F3AF4C7C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti520 – 5201G → R in CAA93280. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69359 Genomic DNA. Translation: CAA93280.1.
    CP004044 Genomic DNA. Translation: AGC68874.1.
    RefSeqiYP_007373484.1. NC_020134.1.

    Genome annotation databases

    EnsemblBacteriaiAGC68874; AGC68874; Cst_c18970.
    GeneIDi14472210.
    KEGGicss:Cst_c18970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z69359 Genomic DNA. Translation: CAA93280.1 .
    CP004044 Genomic DNA. Translation: AGC68874.1 .
    RefSeqi YP_007373484.1. NC_020134.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM3. Carbohydrate-Binding Module Family 3.
    GH48. Glycoside Hydrolase Family 48.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AGC68874 ; AGC68874 ; Cst_c18970 .
    GeneIDi 14472210.
    KEGGi css:Cst_c18970.

    Family and domain databases

    Gene3Di 1.50.10.10. 4 hits.
    2.60.40.10. 1 hit.
    2.60.40.710. 1 hit.
    4.10.870.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR005102. Carbo-bd_X2.
    IPR001956. CBD_3.
    IPR027390. Endoglucanase_F_dom3.
    IPR000556. Glyco_hydro_48F.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    Pfami PF00942. CBM_3. 1 hit.
    PF03442. CBM_X2. 1 hit.
    PF02011. Glyco_hydro_48. 1 hit.
    [Graphical view ]
    PRINTSi PR00844. GLHYDRLASE48.
    SMARTi SM01067. CBM_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49384. SSF49384. 1 hit.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS51172. CBM3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Bronnenmeier K., Kundt K., Riedel K., Schwarz W.H., Staudenbauer W.L.
      Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 35414 / DSM 8532 / NCIMB 11754.
    2. "Complete genome sequence of Clostridium stercorarium subsp. stercorarium strain DSM 8532, a thermophilic degrader of plant cell wall fibers."
      Poehlein A., Zverlov V.V., Daniel R., Schwarz W.H., Liebl W.
      Genome Announc. 1:E0007313-E0007313(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35414 / DSM 8532 / NCIMB 11754.
    3. "Purification and properties of a novel type of exo-1,4-beta-glucanase (avicelase II) from the cellulolytic thermophile Clostridium stercorarium."
      Bronnenmeier K., Ruecknagel K.P., Staudenbauer W.L.
      Eur. J. Biochem. 200:379-385(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: ATCC 35414 / DSM 8532 / NCIMB 11754.

    Entry informationi

    Entry nameiGUX2_CLOSH
    AccessioniPrimary (citable) accession number: P50900
    Secondary accession number(s): L7VQ95
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: June 26, 2013
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3