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P50899

- GUXB_CELFA

UniProt

P50899 - GUXB_CELFA

Protein

Exoglucanase B

Gene

cbhB

Organism
Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Hydrolyzes cellohexaose to a mixture of cellotetraose, cellotriose and cellobiose, with only a trace of glucose. It hydrolyzed cellopentaose to cellotriose and cellobiose, and cellotetraose to cellobiose, but it did not hydrolyze cellotriose. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei513 – 5131NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: InterPro
    2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
    3. polysaccharide binding Source: InterPro

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCFIM590998:GJFK-3447-MONOMER.

    Protein family/group databases

    CAZyiCBM2. Carbohydrate-Binding Module Family 2.
    GH48. Glycoside Hydrolase Family 48.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exoglucanase B (EC:3.2.1.91)
    Alternative name(s):
    1,4-beta-cellobiohydrolase B
    CBP120
    Exocellobiohydrolase B
    Gene namesi
    Name:cbhB
    Synonyms:cenE
    Ordered Locus Names:Celf_3400
    OrganismiCellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
    Taxonomic identifieri590998 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas
    ProteomesiUP000008460: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Propeptidei34 – 53202 PublicationsPRO_0000008028Add
    BLAST
    Chaini54 – 10901037Exoglucanase BPRO_0000008029Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi990 ↔ 1089By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP50899.
    SMRiP50899. Positions 53-694.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini706 – 79186Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini797 – 88791Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini897 – 98488Fibronectin type-III 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini983 – 1090108CBM2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 699646CatalyticBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 3 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    OMAiWLWLEAL.

    Family and domain databases

    Gene3Di1.50.10.10. 4 hits.
    2.60.40.10. 3 hits.
    2.60.40.290. 1 hit.
    4.10.870.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR018366. CBM2_CS.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR027390. Endoglucanase_F_dom3.
    IPR003961. Fibronectin_type3.
    IPR000556. Glyco_hydro_48F.
    IPR013783. Ig-like_fold.
    [Graphical view]
    PfamiPF00553. CBM_2. 1 hit.
    PF00041. fn3. 3 hits.
    PF02011. Glyco_hydro_48. 1 hit.
    [Graphical view]
    PRINTSiPR00844. GLHYDRLASE48.
    SMARTiSM00637. CBD_II. 1 hit.
    SM00060. FN3. 3 hits.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    SSF49265. SSF49265. 2 hits.
    SSF49384. SSF49384. 1 hit.
    PROSITEiPS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS50853. FN3. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50899-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSTTRRRSA WVAAATVGVS SFLAVAGITP AIAAAGAGQP ATVTVPAASP     50
    VRAAVDGEYA QRFLAQYDKI KDPANGYFSA QGIPYHAVET LMVEAPDYGH 100
    ETTSEAYSYW LWLEALYGQV TQDWAPLNHA WDTMEKYMIP QSVDQPTNSF 150
    YNPNSPATYA PEFNHPSSYP SQLNSGISGG TDPIGAELKA TYGNADVYQM 200
    HWLADVDNIY GFGATPGAGC TLGPTATGTS FINTFQRGPQ ESVWETVPQP 250
    SCEEFKYGGK NGYLDLFTKD ASYAKQWKYT SASDADARAV EAVYWANQWA 300
    TEQGKAADVA ATVAKAAKMG DYLRYTLFDK YFKKIGCTSP TCAAGQGREA 350
    AHYLLSWYMA WGGATDTSSG WAWRIGSSHA HFGYQNPLAA WALSTDPKLT 400
    PKSPTAKADW AASMQRQLEF YTWLQASNGG IAGGATNSWD GAYAQPPAGT 450
    PTFYGMGYTE APVYVDPPSN RWFGMQAWGV QRVAELYYAS GNAQAKKILD 500
    KWVPWVVANI STDGASWKVP SELKWTGKPD TWNAAAPTGN PGLTVEVTSY 550
    GQDVGVAADT ARALLFYAAK SGDTASRDKA KALLDAIWAN NQDPLGVSAV 600
    ETRGDYKRFD DTYVANGDGI YIPSGWTGTM PNGDVIKPGV SFLDIRSFYK 650
    KDPNWSKVQT FLDGGAEPQF RYHRFWAQTA VAGALADYAR LFDDGTTTPD 700
    TTAPTVPTGL QAGVVTSTEA TISWTASTDD TRVTGYDVYR GATKVGTATT 750
    TSFTDTGLTA STAYAYTVRA FDAAGNVSAP SAALTVTTKA TPSDTTAPSV 800
    PAITSSSSTA NSVTIGWSAS TDNAGGSGLA GYDVYRGATR VAQTTALTFT 850
    DTGLTASTAY EYTVRARDVA GNVSAPSTAV SVTTKSDTTP DTTAPSVPAG 900
    LAAMTVTETS VALTWNASTD TGGSGLKGYD VYRGATRVGS TTTASYTDTG 950
    LTAATAYQYT VRATDNAGNV SAASAALSVT TKTPQTGGSC SVAYNASSWN 1000
    SGFTASVRIT NTGTTTINGW SLGFDLTAGQ KVQQGWSATW TQSGSTVTAT 1050
    NAPWNGTLAP GQTVDVGFNG SHTGQNPNPA SFTLNGASCT 1090
    Length:1,090
    Mass (Da):114,829
    Last modified:October 1, 1996 - v1
    Checksum:i046BB9D956F2F399
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38827 Genomic DNA. Translation: AAB00822.1.
    CP002666 Genomic DNA. Translation: AEE47513.1.
    PIRiS59077.
    RefSeqiWP_013772537.1. NC_015514.1.
    YP_004454900.1. NC_015514.1.

    Genome annotation databases

    EnsemblBacteriaiAEE47513; AEE47513; Celf_3400.
    GeneIDi10594207.
    KEGGicfi:Celf_3400.
    PATRICi54617797. VBICelFim18861_3405.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L38827 Genomic DNA. Translation: AAB00822.1 .
    CP002666 Genomic DNA. Translation: AEE47513.1 .
    PIRi S59077.
    RefSeqi WP_013772537.1. NC_015514.1.
    YP_004454900.1. NC_015514.1.

    3D structure databases

    ProteinModelPortali P50899.
    SMRi P50899. Positions 53-694.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM2. Carbohydrate-Binding Module Family 2.
    GH48. Glycoside Hydrolase Family 48.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEE47513 ; AEE47513 ; Celf_3400 .
    GeneIDi 10594207.
    KEGGi cfi:Celf_3400.
    PATRICi 54617797. VBICelFim18861_3405.

    Phylogenomic databases

    OMAi WLWLEAL.

    Enzyme and pathway databases

    BioCyci CFIM590998:GJFK-3447-MONOMER.

    Family and domain databases

    Gene3Di 1.50.10.10. 4 hits.
    2.60.40.10. 3 hits.
    2.60.40.290. 1 hit.
    4.10.870.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR008965. Carb-bd_dom.
    IPR012291. CBD_carb-bd_dom.
    IPR018366. CBM2_CS.
    IPR001919. Cellulose-bd_dom_fam2_bac.
    IPR027390. Endoglucanase_F_dom3.
    IPR003961. Fibronectin_type3.
    IPR000556. Glyco_hydro_48F.
    IPR013783. Ig-like_fold.
    [Graphical view ]
    Pfami PF00553. CBM_2. 1 hit.
    PF00041. fn3. 3 hits.
    PF02011. Glyco_hydro_48. 1 hit.
    [Graphical view ]
    PRINTSi PR00844. GLHYDRLASE48.
    SMARTi SM00637. CBD_II. 1 hit.
    SM00060. FN3. 3 hits.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    SSF49265. SSF49265. 2 hits.
    SSF49384. SSF49384. 1 hit.
    PROSITEi PS51173. CBM2. 1 hit.
    PS00561. CBM2_A. 1 hit.
    PS50853. FN3. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi."
      Shen H., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
      Biochem. J. 311:67-74(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 456-461.
      Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
    2. "Complete sequence of Cellulomonas fimi ATCC 484."
      US DOE Joint Genome Institute
      Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Mead D., Brumm P., Woyke T.
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
    3. "Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase."
      Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
      J. Bacteriol. 175:1910-1918(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 54-75.
      Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
    4. "Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of beta-1,4-glucanases."
      Shen H., Tomme P., Meinke A., Gilkes N.R., Kilburn D.G., Warren R.A.J., Miller R.C. Jr.
      Biochem. Biophys. Res. Commun. 199:1223-1228(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 54-78.

    Entry informationi

    Entry nameiGUXB_CELFA
    AccessioniPrimary (citable) accession number: P50899
    Secondary accession number(s): F4H1U3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3