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P50899 (GUXB_CELFA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exoglucanase B

EC=3.2.1.91
Alternative name(s):
1,4-beta-cellobiohydrolase B
CBP120
Exocellobiohydrolase B
Gene names
Name:cbhB
Synonyms:cenE
Ordered Locus Names:Celf_3400
OrganismCellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) [Complete proteome] [HAMAP]
Taxonomic identifier590998 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas

Protein attributes

Sequence length1090 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes cellohexaose to a mixture of cellotetraose, cellotriose and cellobiose, with only a trace of glucose. It hydrolyzed cellopentaose to cellotriose and cellobiose, and cellotetraose to cellobiose, but it did not hydrolyze cellotriose. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sequence similarities

Belongs to the glycosyl hydrolase 48 (cellulase L) family.

Contains 1 CBM2 (carbohydrate binding type-2) domain.

Contains 3 fibronectin type-III domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Propeptide34 – 5320
PRO_0000008028
Chain54 – 10901037Exoglucanase B
PRO_0000008029

Regions

Domain706 – 79186Fibronectin type-III 1
Domain797 – 88791Fibronectin type-III 2
Domain897 – 98488Fibronectin type-III 3
Domain983 – 1090108CBM2
Region54 – 699646Catalytic By similarity

Sites

Active site5131Nucleophile By similarity

Amino acid modifications

Disulfide bond990 ↔ 1089 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50899 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 046BB9D956F2F399

FASTA1,090114,829
        10         20         30         40         50         60 
MSSTTRRRSA WVAAATVGVS SFLAVAGITP AIAAAGAGQP ATVTVPAASP VRAAVDGEYA 

        70         80         90        100        110        120 
QRFLAQYDKI KDPANGYFSA QGIPYHAVET LMVEAPDYGH ETTSEAYSYW LWLEALYGQV 

       130        140        150        160        170        180 
TQDWAPLNHA WDTMEKYMIP QSVDQPTNSF YNPNSPATYA PEFNHPSSYP SQLNSGISGG 

       190        200        210        220        230        240 
TDPIGAELKA TYGNADVYQM HWLADVDNIY GFGATPGAGC TLGPTATGTS FINTFQRGPQ 

       250        260        270        280        290        300 
ESVWETVPQP SCEEFKYGGK NGYLDLFTKD ASYAKQWKYT SASDADARAV EAVYWANQWA 

       310        320        330        340        350        360 
TEQGKAADVA ATVAKAAKMG DYLRYTLFDK YFKKIGCTSP TCAAGQGREA AHYLLSWYMA 

       370        380        390        400        410        420 
WGGATDTSSG WAWRIGSSHA HFGYQNPLAA WALSTDPKLT PKSPTAKADW AASMQRQLEF 

       430        440        450        460        470        480 
YTWLQASNGG IAGGATNSWD GAYAQPPAGT PTFYGMGYTE APVYVDPPSN RWFGMQAWGV 

       490        500        510        520        530        540 
QRVAELYYAS GNAQAKKILD KWVPWVVANI STDGASWKVP SELKWTGKPD TWNAAAPTGN 

       550        560        570        580        590        600 
PGLTVEVTSY GQDVGVAADT ARALLFYAAK SGDTASRDKA KALLDAIWAN NQDPLGVSAV 

       610        620        630        640        650        660 
ETRGDYKRFD DTYVANGDGI YIPSGWTGTM PNGDVIKPGV SFLDIRSFYK KDPNWSKVQT 

       670        680        690        700        710        720 
FLDGGAEPQF RYHRFWAQTA VAGALADYAR LFDDGTTTPD TTAPTVPTGL QAGVVTSTEA 

       730        740        750        760        770        780 
TISWTASTDD TRVTGYDVYR GATKVGTATT TSFTDTGLTA STAYAYTVRA FDAAGNVSAP 

       790        800        810        820        830        840 
SAALTVTTKA TPSDTTAPSV PAITSSSSTA NSVTIGWSAS TDNAGGSGLA GYDVYRGATR 

       850        860        870        880        890        900 
VAQTTALTFT DTGLTASTAY EYTVRARDVA GNVSAPSTAV SVTTKSDTTP DTTAPSVPAG 

       910        920        930        940        950        960 
LAAMTVTETS VALTWNASTD TGGSGLKGYD VYRGATRVGS TTTASYTDTG LTAATAYQYT 

       970        980        990       1000       1010       1020 
VRATDNAGNV SAASAALSVT TKTPQTGGSC SVAYNASSWN SGFTASVRIT NTGTTTINGW 

      1030       1040       1050       1060       1070       1080 
SLGFDLTAGQ KVQQGWSATW TQSGSTVTAT NAPWNGTLAP GQTVDVGFNG SHTGQNPNPA 

      1090 
SFTLNGASCT 

« Hide

References

« Hide 'large scale' references
[1]"Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi."
Shen H., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
Biochem. J. 311:67-74(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 456-461.
Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[2]"Complete sequence of Cellulomonas fimi ATCC 484."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Mead D., Brumm P., Woyke T.
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[3]"Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase."
Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
J. Bacteriol. 175:1910-1918(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 54-75.
Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
[4]"Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of beta-1,4-glucanases."
Shen H., Tomme P., Meinke A., Gilkes N.R., Kilburn D.G., Warren R.A.J., Miller R.C. Jr.
Biochem. Biophys. Res. Commun. 199:1223-1228(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 54-78.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L38827 Genomic DNA. Translation: AAB00822.1.
CP002666 Genomic DNA. Translation: AEE47513.1.
PIRS59077.
RefSeqYP_004454900.1. NC_015514.1.

3D structure databases

ProteinModelPortalP50899.
SMRP50899. Positions 53-694.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM2. Carbohydrate-Binding Module Family 2.
GH48. Glycoside Hydrolase Family 48.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEE47513; AEE47513; Celf_3400.
GeneID10594207.
KEGGcfi:Celf_3400.
PATRIC54617797. VBICelFim18861_3405.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAWLWLEAL.

Enzyme and pathway databases

BioCycCFIM590998:GJFK-3447-MONOMER.

Family and domain databases

Gene3D1.50.10.10. 4 hits.
2.60.40.10. 3 hits.
2.60.40.290. 1 hit.
4.10.870.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR018366. CBM2_CS.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR027390. Endoglucanase_F_dom3.
IPR003961. Fibronectin_type3.
IPR000556. Glyco_hydro_48F.
IPR013783. Ig-like_fold.
[Graphical view]
PfamPF00553. CBM_2. 1 hit.
PF00041. fn3. 3 hits.
PF02011. Glyco_hydro_48. 1 hit.
[Graphical view]
PRINTSPR00844. GLHYDRLASE48.
SMARTSM00637. CBD_II. 1 hit.
SM00060. FN3. 3 hits.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF49384. SSF49384. 1 hit.
PROSITEPS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS50853. FN3. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUXB_CELFA
AccessionPrimary (citable) accession number: P50899
Secondary accession number(s): F4H1U3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: December 11, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries