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P50899

- GUXB_CELFA

UniProt

P50899 - GUXB_CELFA

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Protein

Exoglucanase B

Gene
cbhB, cenE, Celf_3400
Organism
Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes cellohexaose to a mixture of cellotetraose, cellotriose and cellobiose, with only a trace of glucose. It hydrolyzed cellopentaose to cellotriose and cellobiose, and cellotetraose to cellobiose, but it did not hydrolyze cellotriose. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei513 – 5131Nucleophile By similarity

GO - Molecular functioni

  1. cellulase activity Source: InterPro
  2. cellulose 1,4-beta-cellobiosidase activity Source: UniProtKB-EC
  3. polysaccharide binding Source: InterPro

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCFIM590998:GJFK-3447-MONOMER.

Protein family/group databases

CAZyiCBM2. Carbohydrate-Binding Module Family 2.
GH48. Glycoside Hydrolase Family 48.

Names & Taxonomyi

Protein namesi
Recommended name:
Exoglucanase B (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase B
CBP120
Exocellobiohydrolase B
Gene namesi
Name:cbhB
Synonyms:cenE
Ordered Locus Names:Celf_3400
OrganismiCellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Taxonomic identifieri590998 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeCellulomonadaceaeCellulomonas
ProteomesiUP000008460: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333 Reviewed predictionAdd
BLAST
Propeptidei34 – 5320PRO_0000008028Add
BLAST
Chaini54 – 10901037Exoglucanase BPRO_0000008029Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi990 ↔ 1089 By similarity

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP50899.
SMRiP50899. Positions 53-694.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini706 – 79186Fibronectin type-III 1Add
BLAST
Domaini797 – 88791Fibronectin type-III 2Add
BLAST
Domaini897 – 98488Fibronectin type-III 3Add
BLAST
Domaini983 – 1090108CBM2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 699646Catalytic By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

OMAiWLWLEAL.

Family and domain databases

Gene3Di1.50.10.10. 4 hits.
2.60.40.10. 3 hits.
2.60.40.290. 1 hit.
4.10.870.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR018366. CBM2_CS.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR027390. Endoglucanase_F_dom3.
IPR003961. Fibronectin_type3.
IPR000556. Glyco_hydro_48F.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00553. CBM_2. 1 hit.
PF00041. fn3. 3 hits.
PF02011. Glyco_hydro_48. 1 hit.
[Graphical view]
PRINTSiPR00844. GLHYDRLASE48.
SMARTiSM00637. CBD_II. 1 hit.
SM00060. FN3. 3 hits.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF49384. SSF49384. 1 hit.
PROSITEiPS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS50853. FN3. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50899-1 [UniParc]FASTAAdd to Basket

« Hide

MSSTTRRRSA WVAAATVGVS SFLAVAGITP AIAAAGAGQP ATVTVPAASP     50
VRAAVDGEYA QRFLAQYDKI KDPANGYFSA QGIPYHAVET LMVEAPDYGH 100
ETTSEAYSYW LWLEALYGQV TQDWAPLNHA WDTMEKYMIP QSVDQPTNSF 150
YNPNSPATYA PEFNHPSSYP SQLNSGISGG TDPIGAELKA TYGNADVYQM 200
HWLADVDNIY GFGATPGAGC TLGPTATGTS FINTFQRGPQ ESVWETVPQP 250
SCEEFKYGGK NGYLDLFTKD ASYAKQWKYT SASDADARAV EAVYWANQWA 300
TEQGKAADVA ATVAKAAKMG DYLRYTLFDK YFKKIGCTSP TCAAGQGREA 350
AHYLLSWYMA WGGATDTSSG WAWRIGSSHA HFGYQNPLAA WALSTDPKLT 400
PKSPTAKADW AASMQRQLEF YTWLQASNGG IAGGATNSWD GAYAQPPAGT 450
PTFYGMGYTE APVYVDPPSN RWFGMQAWGV QRVAELYYAS GNAQAKKILD 500
KWVPWVVANI STDGASWKVP SELKWTGKPD TWNAAAPTGN PGLTVEVTSY 550
GQDVGVAADT ARALLFYAAK SGDTASRDKA KALLDAIWAN NQDPLGVSAV 600
ETRGDYKRFD DTYVANGDGI YIPSGWTGTM PNGDVIKPGV SFLDIRSFYK 650
KDPNWSKVQT FLDGGAEPQF RYHRFWAQTA VAGALADYAR LFDDGTTTPD 700
TTAPTVPTGL QAGVVTSTEA TISWTASTDD TRVTGYDVYR GATKVGTATT 750
TSFTDTGLTA STAYAYTVRA FDAAGNVSAP SAALTVTTKA TPSDTTAPSV 800
PAITSSSSTA NSVTIGWSAS TDNAGGSGLA GYDVYRGATR VAQTTALTFT 850
DTGLTASTAY EYTVRARDVA GNVSAPSTAV SVTTKSDTTP DTTAPSVPAG 900
LAAMTVTETS VALTWNASTD TGGSGLKGYD VYRGATRVGS TTTASYTDTG 950
LTAATAYQYT VRATDNAGNV SAASAALSVT TKTPQTGGSC SVAYNASSWN 1000
SGFTASVRIT NTGTTTINGW SLGFDLTAGQ KVQQGWSATW TQSGSTVTAT 1050
NAPWNGTLAP GQTVDVGFNG SHTGQNPNPA SFTLNGASCT 1090
Length:1,090
Mass (Da):114,829
Last modified:October 1, 1996 - v1
Checksum:i046BB9D956F2F399
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L38827 Genomic DNA. Translation: AAB00822.1.
CP002666 Genomic DNA. Translation: AEE47513.1.
PIRiS59077.
RefSeqiWP_013772537.1. NC_015514.1.
YP_004454900.1. NC_015514.1.

Genome annotation databases

EnsemblBacteriaiAEE47513; AEE47513; Celf_3400.
GeneIDi10594207.
KEGGicfi:Celf_3400.
PATRICi54617797. VBICelFim18861_3405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L38827 Genomic DNA. Translation: AAB00822.1 .
CP002666 Genomic DNA. Translation: AEE47513.1 .
PIRi S59077.
RefSeqi WP_013772537.1. NC_015514.1.
YP_004454900.1. NC_015514.1.

3D structure databases

ProteinModelPortali P50899.
SMRi P50899. Positions 53-694.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM2. Carbohydrate-Binding Module Family 2.
GH48. Glycoside Hydrolase Family 48.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEE47513 ; AEE47513 ; Celf_3400 .
GeneIDi 10594207.
KEGGi cfi:Celf_3400.
PATRICi 54617797. VBICelFim18861_3405.

Phylogenomic databases

OMAi WLWLEAL.

Enzyme and pathway databases

BioCyci CFIM590998:GJFK-3447-MONOMER.

Family and domain databases

Gene3Di 1.50.10.10. 4 hits.
2.60.40.10. 3 hits.
2.60.40.290. 1 hit.
4.10.870.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR012291. CBD_carb-bd_dom.
IPR018366. CBM2_CS.
IPR001919. Cellulose-bd_dom_fam2_bac.
IPR027390. Endoglucanase_F_dom3.
IPR003961. Fibronectin_type3.
IPR000556. Glyco_hydro_48F.
IPR013783. Ig-like_fold.
[Graphical view ]
Pfami PF00553. CBM_2. 1 hit.
PF00041. fn3. 3 hits.
PF02011. Glyco_hydro_48. 1 hit.
[Graphical view ]
PRINTSi PR00844. GLHYDRLASE48.
SMARTi SM00637. CBD_II. 1 hit.
SM00060. FN3. 3 hits.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF49384. SSF49384. 1 hit.
PROSITEi PS51173. CBM2. 1 hit.
PS00561. CBM2_A. 1 hit.
PS50853. FN3. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi."
    Shen H., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
    Biochem. J. 311:67-74(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 456-461.
    Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
  2. "Complete sequence of Cellulomonas fimi ATCC 484."
    US DOE Joint Genome Institute
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Mead D., Brumm P., Woyke T.
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
  3. "Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase."
    Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
    J. Bacteriol. 175:1910-1918(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 54-75.
    Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.
  4. "Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of beta-1,4-glucanases."
    Shen H., Tomme P., Meinke A., Gilkes N.R., Kilburn D.G., Warren R.A.J., Miller R.C. Jr.
    Biochem. Biophys. Res. Commun. 199:1223-1228(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 54-78.

Entry informationi

Entry nameiGUXB_CELFA
AccessioniPrimary (citable) accession number: P50899
Secondary accession number(s): F4H1U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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