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Protein

Exoglucanase B

Gene

cbhB

Organism
Cellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes cellohexaose to a mixture of cellotetraose, cellotriose and cellobiose, with only a trace of glucose. It hydrolyzed cellopentaose to cellotriose and cellobiose, and cellotetraose to cellobiose, but it did not hydrolyze cellotriose. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei513NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCFIM590998:G1GXC-3432-MONOMER

Protein family/group databases

CAZyiCBM2 Carbohydrate-Binding Module Family 2
GH48 Glycoside Hydrolase Family 48

Names & Taxonomyi

Protein namesi
Recommended name:
Exoglucanase B (EC:3.2.1.91)
Alternative name(s):
1,4-beta-cellobiohydrolase B
CBP120
Exocellobiohydrolase B
Gene namesi
Name:cbhB
Synonyms:cenE
Ordered Locus Names:Celf_3400
OrganismiCellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547)
Taxonomic identifieri590998 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesCellulomonadaceaeCellulomonas
Proteomesi
  • UP000008460 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
PropeptideiPRO_000000802834 – 532 PublicationsAdd BLAST20
ChainiPRO_000000802954 – 1090Exoglucanase BAdd BLAST1037

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi990 ↔ 1089By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi590998.Celf_3400

Structurei

3D structure databases

ProteinModelPortaliP50899
SMRiP50899
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini706 – 791Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST86
Domaini797 – 887Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST91
Domaini897 – 984Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST88
Domaini983 – 1090CBM2PROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 699CatalyticBy similarityAdd BLAST646

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4105CYP Bacteria
ENOG410XPC9 LUCA
OMAiWLWLEAL
OrthoDBiPOG091H0EGP

Family and domain databases

CDDicd00063 FN3, 3 hits
Gene3Di1.50.10.10, 5 hits
2.170.160.10, 3 hits
2.60.40.10, 3 hits
2.60.40.290, 1 hit
4.10.870.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR001919 CBD2
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR012291 CBM2_carb-bd_dom_sf
IPR018366 CBM2_CS
IPR023309 Endo-1-4-beta-glucanase_dom2
IPR027390 Endoglucanase_F_dom3
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR000556 Glyco_hydro_48F
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00553 CBM_2, 1 hit
PF00041 fn3, 2 hits
PF02011 Glyco_hydro_48, 1 hit
PRINTSiPR00844 GLHYDRLASE48
SMARTiView protein in SMART
SM00637 CBD_II, 1 hit
SM00060 FN3, 3 hits
SUPFAMiSSF48208 SSF48208, 1 hit
SSF49265 SSF49265, 2 hits
SSF49384 SSF49384, 1 hit
PROSITEiView protein in PROSITE
PS51173 CBM2, 1 hit
PS00561 CBM2_A, 1 hit
PS50853 FN3, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50899-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTTRRRSA WVAAATVGVS SFLAVAGITP AIAAAGAGQP ATVTVPAASP
60 70 80 90 100
VRAAVDGEYA QRFLAQYDKI KDPANGYFSA QGIPYHAVET LMVEAPDYGH
110 120 130 140 150
ETTSEAYSYW LWLEALYGQV TQDWAPLNHA WDTMEKYMIP QSVDQPTNSF
160 170 180 190 200
YNPNSPATYA PEFNHPSSYP SQLNSGISGG TDPIGAELKA TYGNADVYQM
210 220 230 240 250
HWLADVDNIY GFGATPGAGC TLGPTATGTS FINTFQRGPQ ESVWETVPQP
260 270 280 290 300
SCEEFKYGGK NGYLDLFTKD ASYAKQWKYT SASDADARAV EAVYWANQWA
310 320 330 340 350
TEQGKAADVA ATVAKAAKMG DYLRYTLFDK YFKKIGCTSP TCAAGQGREA
360 370 380 390 400
AHYLLSWYMA WGGATDTSSG WAWRIGSSHA HFGYQNPLAA WALSTDPKLT
410 420 430 440 450
PKSPTAKADW AASMQRQLEF YTWLQASNGG IAGGATNSWD GAYAQPPAGT
460 470 480 490 500
PTFYGMGYTE APVYVDPPSN RWFGMQAWGV QRVAELYYAS GNAQAKKILD
510 520 530 540 550
KWVPWVVANI STDGASWKVP SELKWTGKPD TWNAAAPTGN PGLTVEVTSY
560 570 580 590 600
GQDVGVAADT ARALLFYAAK SGDTASRDKA KALLDAIWAN NQDPLGVSAV
610 620 630 640 650
ETRGDYKRFD DTYVANGDGI YIPSGWTGTM PNGDVIKPGV SFLDIRSFYK
660 670 680 690 700
KDPNWSKVQT FLDGGAEPQF RYHRFWAQTA VAGALADYAR LFDDGTTTPD
710 720 730 740 750
TTAPTVPTGL QAGVVTSTEA TISWTASTDD TRVTGYDVYR GATKVGTATT
760 770 780 790 800
TSFTDTGLTA STAYAYTVRA FDAAGNVSAP SAALTVTTKA TPSDTTAPSV
810 820 830 840 850
PAITSSSSTA NSVTIGWSAS TDNAGGSGLA GYDVYRGATR VAQTTALTFT
860 870 880 890 900
DTGLTASTAY EYTVRARDVA GNVSAPSTAV SVTTKSDTTP DTTAPSVPAG
910 920 930 940 950
LAAMTVTETS VALTWNASTD TGGSGLKGYD VYRGATRVGS TTTASYTDTG
960 970 980 990 1000
LTAATAYQYT VRATDNAGNV SAASAALSVT TKTPQTGGSC SVAYNASSWN
1010 1020 1030 1040 1050
SGFTASVRIT NTGTTTINGW SLGFDLTAGQ KVQQGWSATW TQSGSTVTAT
1060 1070 1080 1090
NAPWNGTLAP GQTVDVGFNG SHTGQNPNPA SFTLNGASCT
Length:1,090
Mass (Da):114,829
Last modified:October 1, 1996 - v1
Checksum:i046BB9D956F2F399
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38827 Genomic DNA Translation: AAB00822.1
CP002666 Genomic DNA Translation: AEE47513.1
PIRiS59077
RefSeqiWP_013772537.1, NC_015514.1

Genome annotation databases

EnsemblBacteriaiAEE47513; AEE47513; Celf_3400
KEGGicfi:Celf_3400

Similar proteinsi

Entry informationi

Entry nameiGUXB_CELFA
AccessioniPrimary (citable) accession number: P50899
Secondary accession number(s): F4H1U3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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