Reviewed, UniProtKB/Swiss-Prot P50899 (GUXB_CELFI)

Names and origin

Protein names

Recommended name:
    Exoglucanase B
    EC=3.2.1.91
Alternative name(s):
    Exocellobiohydrolase B
    1,4-beta-cellobiohydrolase B
    CBP120

Gene names

Name:	cbhB
Synonyms:	cenE

Organism

Cellulomonas fimi

Taxonomic identifier

1708 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Cellulomonadaceae › Cellulomonas

Protein attributes

Sequence length	1090 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes cellohexaose to a mixture of cellotetraose, cellotriose and cellobiose, with only a trace of glucose. It hydrolyzed cellopentaose to cellotriose and cellobiose, and cellotetraose to cellobiose, but it did not hydrolyze cellotriose. Has also weak endoglucanase activity. Hydrolyzes glucosidic bonds with inversion of anomeric configuration.
Catalytic activity	Hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 48 (cellulase L) family. Contains 1 CBM2 (carbohydrate binding type-2) domain. Contains 3 fibronectin type-III domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC polysaccharide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Propeptide	34 – 53	20	Ref.2 Ref.3		PRO_0000008028
	Chain	54 – 1090	1037	Exoglucanase B		PRO_0000008029
Regions
	Domain	704 – 787	84	Fibronectin type-III 1
	Domain	794 – 883	90	Fibronectin type-III 2
	Domain	895 – 981	87	Fibronectin type-III 3
	Domain	983 – 1090	108	CBM2
	Region	54 – 699	646	Catalytic By similarity
Sites
	Active site	513	1	Nucleophile By similarity
Amino acid modifications
	Disulfide bond	990 ↔ 1089		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50899-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 046BB9D956F2F399	FASTA	1,090	114,829
	10 20 30 40 50 60 MSSTTRRRSA WVAAATVGVS SFLAVAGITP AIAAAGAGQP ATVTVPAASP VRAAVDGEYA 70 80 90 100 110 120 QRFLAQYDKI KDPANGYFSA QGIPYHAVET LMVEAPDYGH ETTSEAYSYW LWLEALYGQV 130 140 150 160 170 180 TQDWAPLNHA WDTMEKYMIP QSVDQPTNSF YNPNSPATYA PEFNHPSSYP SQLNSGISGG 190 200 210 220 230 240 TDPIGAELKA TYGNADVYQM HWLADVDNIY GFGATPGAGC TLGPTATGTS FINTFQRGPQ 250 260 270 280 290 300 ESVWETVPQP SCEEFKYGGK NGYLDLFTKD ASYAKQWKYT SASDADARAV EAVYWANQWA 310 320 330 340 350 360 TEQGKAADVA ATVAKAAKMG DYLRYTLFDK YFKKIGCTSP TCAAGQGREA AHYLLSWYMA 370 380 390 400 410 420 WGGATDTSSG WAWRIGSSHA HFGYQNPLAA WALSTDPKLT PKSPTAKADW AASMQRQLEF 430 440 450 460 470 480 YTWLQASNGG IAGGATNSWD GAYAQPPAGT PTFYGMGYTE APVYVDPPSN RWFGMQAWGV 490 500 510 520 530 540 QRVAELYYAS GNAQAKKILD KWVPWVVANI STDGASWKVP SELKWTGKPD TWNAAAPTGN 550 560 570 580 590 600 PGLTVEVTSY GQDVGVAADT ARALLFYAAK SGDTASRDKA KALLDAIWAN NQDPLGVSAV 610 620 630 640 650 660 ETRGDYKRFD DTYVANGDGI YIPSGWTGTM PNGDVIKPGV SFLDIRSFYK KDPNWSKVQT 670 680 690 700 710 720 FLDGGAEPQF RYHRFWAQTA VAGALADYAR LFDDGTTTPD TTAPTVPTGL QAGVVTSTEA 730 740 750 760 770 780 TISWTASTDD TRVTGYDVYR GATKVGTATT TSFTDTGLTA STAYAYTVRA FDAAGNVSAP 790 800 810 820 830 840 SAALTVTTKA TPSDTTAPSV PAITSSSSTA NSVTIGWSAS TDNAGGSGLA GYDVYRGATR 850 860 870 880 890 900 VAQTTALTFT DTGLTASTAY EYTVRARDVA GNVSAPSTAV SVTTKSDTTP DTTAPSVPAG 910 920 930 940 950 960 LAAMTVTETS VALTWNASTD TGGSGLKGYD VYRGATRVGS TTTASYTDTG LTAATAYQYT 970 980 990 1000 1010 1020 VRATDNAGNV SAASAALSVT TKTPQTGGSC SVAYNASSWN SGFTASVRIT NTGTTTINGW 1030 1040 1050 1060 1070 1080 SLGFDLTAGQ KVQQGWSATW TQSGSTVTAT NAPWNGTLAP GQTVDVGFNG SHTGQNPNPA 1090 SFTLNGASCT

References

[1]	"Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi." Shen H., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J. Biochem. J. 311:67-74(1995) [PubMed: 7575482] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 456-461. Strain: ATCC 484 / DSM 20113 / IFO 15513 / JCM 1341 / NCTC 7547.
[2]	"Cellulose-binding polypeptides from Cellulomonas fimi: endoglucanase D (CenD), a family A beta-1,4-glucanase." Meinke A., Gilkes N.R., Kilburn D.G., Miller R.C. Jr., Warren R.A.J. J. Bacteriol. 175:1910-1918(1993) [PubMed: 8458833] [Abstract] Cited for: PROTEIN SEQUENCE OF 54-75.
[3]	"Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of beta-1,4-glucanases." Shen H., Tomme P., Meinke A., Gilkes N.R., Kilburn D.G., Warren R.A.J., Miller R.C. Jr. Biochem. Biophys. Res. Commun. 199:1223-1228(1994) [PubMed: 8147863] [Abstract] Cited for: PROTEIN SEQUENCE OF 54-78.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L38827 Genomic DNA. Translation: AAB00822.1.
PIR	S59077.
3D structure databases
HSSP	HSSP built from PDB template 1FAE based on UniProtKB P37698.
ModBase	Search...
Protein family/group databases
CAZy	CBM2. Carbohydrate-Binding Module Family 2. GH48. Glycoside Hydrolase Family 48.
Enzyme and pathway databases
BRENDA	3.2.1.91. 97800.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001919. CBD_bac. IPR012291. CBD_carb_bd. IPR018366. CBM2_CS. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR000556. Glyco_hydro_48F. [Graphical view]
Gene3D	G3DSA:2.60.40.290. CBD_carb_bd. 1 hit. G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit. G3DSA:2.60.40.30. FN_III-like. 3 hits.
Pfam	PF00553. CBM_2. 1 hit. PF00041. fn3. 3 hits. PF02011. Glyco_hydro_48. 1 hit. [Graphical view]
PRINTS	PR00844. GLHYDRLASE48.
ProDom	PD011903. Glyco_hydro_48. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00637. CBD_II. 1 hit. SM00060. FN3. 3 hits. [Graphical view]
PROSITE	PS51173. CBM2. 1 hit. PS00561. CBM2_A. 1 hit. PS50853. FN3. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUXB_CELFI

Accession

Primary (citable) accession number: P50899

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 5, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)