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Protein

Basal cell adhesion molecule

Gene

BCAM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Laminin alpha-5 receptor. May mediate intracellular signaling.1 Publication

GO - Molecular functioni

  • laminin binding Source: BHF-UCL
  • laminin receptor activity Source: BHF-UCL
  • protein C-terminus binding Source: CACAO
  • transmembrane signaling receptor activity Source: ProtInc

GO - Biological processi

  • cell adhesion Source: ProtInc
  • cell-matrix adhesion Source: BHF-UCL
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Blood group antigen, Receptor

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

BioCyciZFISH:ENSG00000142232-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Basal cell adhesion molecule
Alternative name(s):
Auberger B antigen
B-CAM cell surface glycoprotein
F8/G253 antigen
Lutheran antigen
Lutheran blood group glycoprotein
CD_antigen: CD239
Gene namesi
Name:BCAM
Synonyms:LU, MSK19
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:6722. BCAM.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini32 – 547ExtracellularSequence analysisAdd BLAST516
Transmembranei548 – 568HelicalSequence analysisAdd BLAST21
Topological domaini569 – 628CytoplasmicSequence analysisAdd BLAST60

GO - Cellular componenti

  • external side of plasma membrane Source: BHF-UCL
  • extracellular exosome Source: UniProtKB
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi621S → A: Dramatically reduced cell adhesion. 1 Publication1

Organism-specific databases

DisGeNETi4059.
MIMi111200. phenotype.
247420. phenotype.
OpenTargetsiENSG00000187244.
PharmGKBiPA30484.

Polymorphism and mutation databases

BioMutaiBCAM.
DMDMi92058724.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 311 PublicationAdd BLAST31
ChainiPRO_000001485032 – 628Basal cell adhesion moleculeAdd BLAST597

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 125PROSITE-ProRule annotation1 Publication
Disulfide bondi172 ↔ 237PROSITE-ProRule annotation1 Publication
Disulfide bondi291 ↔ 3371 Publication
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Glycosylationi377N-linked (GlcNAc...)Sequence analysis1
Glycosylationi383N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi384 ↔ 4241 Publication
Glycosylationi419N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)2 Publications1
Disulfide bondi473 ↔ 5221 Publication
Modified residuei596Phosphoserine; by GSK31 Publication1
Modified residuei598Phosphoserine; by CK21 Publication1
Modified residuei600PhosphoserineCombined sources1
Modified residuei621Phosphoserine; by PKA1 Publication1

Post-translational modificationi

Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP50895.
MaxQBiP50895.
PaxDbiP50895.
PeptideAtlasiP50895.
PRIDEiP50895.

PTM databases

iPTMnetiP50895.
PhosphoSitePlusiP50895.
SwissPalmiP50895.

Expressioni

Tissue specificityi

Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.

Developmental stagei

Is under developmental control in liver and may also be regulated during differentiation in other tissues. Up-regulated following malignant transformation in some cell types.

Gene expression databases

BgeeiENSG00000187244.
CleanExiHS_BCAM.
ExpressionAtlasiP50895. baseline and differential.
GenevisibleiP50895. HS.

Organism-specific databases

HPAiHPA005654.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KLHL2O951983EBI-10212133,EBI-746999

GO - Molecular functioni

  • laminin binding Source: BHF-UCL
  • protein C-terminus binding Source: CACAO

Protein-protein interaction databases

BioGridi110237. 19 interactors.
IntActiP50895. 5 interactors.
MINTiMINT-5004331.
STRINGi9606.ENSP00000270233.

Structurei

Secondary structure

1628
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 36Combined sources3
Beta strandi39 – 44Combined sources6
Beta strandi49 – 51Combined sources3
Beta strandi54 – 57Combined sources4
Beta strandi60 – 69Combined sources10
Beta strandi77 – 84Combined sources8
Beta strandi87 – 92Combined sources6
Beta strandi110 – 114Combined sources5
Helixi117 – 119Combined sources3
Beta strandi121 – 128Combined sources8
Helixi130 – 132Combined sources3
Beta strandi134 – 145Combined sources12
Beta strandi151 – 154Combined sources4
Beta strandi167 – 179Combined sources13
Beta strandi182 – 187Combined sources6
Beta strandi190 – 192Combined sources3
Beta strandi200 – 210Combined sources11
Beta strandi216 – 224Combined sources9
Helixi228 – 232Combined sources5
Beta strandi234 – 242Combined sources9
Helixi244 – 246Combined sources3
Beta strandi248 – 252Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PETX-ray1.70A32-262[»]
2PF6X-ray2.20A/B32-262[»]
ProteinModelPortaliP50895.
SMRiP50895.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50895.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 142Ig-like V-type 1Add BLAST111
Domaini147 – 257Ig-like V-type 2Add BLAST111
Domaini274 – 355Ig-like C2-type 1Add BLAST82
Domaini363 – 441Ig-like C2-type 2Add BLAST79
Domaini448 – 541Ig-like C2-type 3Add BLAST94

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni309 – 312Interaction with laminin alpha54

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJIS. Eukaryota.
ENOG4111XZT. LUCA.
GeneTreeiENSGT00530000063457.
HOGENOMiHOG000113409.
InParanoidiP50895.
KOiK06578.
OMAiWFLTDRS.
OrthoDBiEOG091G05IA.
PhylomeDBiP50895.
TreeFamiTF330534.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF13895. Ig_2. 2 hits.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 5 hits.
SM00408. IGc2. 3 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiPS50835. IG_LIKE. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50895-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPPDAPAQA RGAPRLLLLA VLLAAHPDAQ AEVRLSVPPL VEVMRGKSVI
60 70 80 90 100
LDCTPTGTHD HYMLEWFLTD RSGARPRLAS AEMQGSELQV TMHDTRGRSP
110 120 130 140 150
PYQLDSQGRL VLAEAQVGDE RDYVCVVRAG AAGTAEATAR LNVFAKPEAT
160 170 180 190 200
EVSPNKGTLS VMEDSAQEIA TCNSRNGNPA PKITWYRNGQ RLEVPVEMNP
210 220 230 240 250
EGYMTSRTVR EASGLLSLTS TLYLRLRKDD RDASFHCAAH YSLPEGRHGR
260 270 280 290 300
LDSPTFHLTL HYPTEHVQFW VGSPSTPAGW VREGDTVQLL CRGDGSPSPE
310 320 330 340 350
YTLFRLQDEQ EEVLNVNLEG NLTLEGVTRG QSGTYGCRVE DYDAADDVQL
360 370 380 390 400
SKTLELRVAY LDPLELSEGK VLSLPLNSSA VVNCSVHGLP TPALRWTKDS
410 420 430 440 450
TPLGDGPMLS LSSITFDSNG TYVCEASLPT VPVLSRTQNF TLLVQGSPEL
460 470 480 490 500
KTAEIEPKAD GSWREGDEVT LICSARGHPD PKLSWSQLGG SPAEPIPGRQ
510 520 530 540 550
GWVSSSLTLK VTSALSRDGI SCEASNPHGN KRHVFHFGTV SPQTSQAGVA
560 570 580 590 600
VMAVAVSVGL LLLVVAVFYC VRRKGGPCCR QRREKGAPPP GEPGLSHSGS
610 620
EQPEQTGLLM GGASGGARGG SGGFGDEC
Length:628
Mass (Da):67,405
Last modified:March 7, 2006 - v2
Checksum:iC88F4F5C640C3F5B
GO

Sequence cautioni

The sequence EAW57297 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225 – 226RL → PC in CAA56327 (PubMed:7954395).Curated2
Sequence conflicti355 – 356EL → DV in CAA56327 (PubMed:7954395).Curated2
Sequence conflicti532R → L in ABY27636 (Ref. 7) Curated1

Polymorphismi

BCAM is responsible for the Lutheran blood group system (LU) [MIMi:111200]. Lutheran is a complex blood group system consisting of 19 antigens. Antigens Lu(a) and Lu(b) are defined by a polymorphism at position 77: Lu(a) has His-77 and Lu(b) has Arg-77.2 Publications
Inactivating variants in BCAM are responsible for the recessive Lutheran null phenotype Lu(a-b-) of the Lutheran blood group [MIMi:247420]. Autosomal recessive inheritance of the Lutheran null blood group phenotype is extremely rare. There is no obvious associated clinical or hematologic pathology, and all patients have been identified through identification of anti-Lu3 antibodies in their serum.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02134877R → H Defines the Lu(a) antigen. 1 PublicationCorresponds to variant rs28399653dbSNPEnsembl.1
Natural variantiVAR_021349196V → I.1 PublicationCorresponds to variant rs28399654dbSNPEnsembl.1
Natural variantiVAR_021350204M → K.1 PublicationCorresponds to variant rs28399656dbSNPEnsembl.1
Natural variantiVAR_021351282R → H.1 PublicationCorresponds to variant rs9967601dbSNPEnsembl.1
Natural variantiVAR_021352381V → I.1 PublicationCorresponds to variant rs28399626dbSNPEnsembl.1
Natural variantiVAR_021353451K → Q.1 PublicationCorresponds to variant rs28399630dbSNPEnsembl.1
Natural variantiVAR_021354539T → A.3 PublicationsCorresponds to variant rs1135062dbSNPEnsembl.1
Natural variantiVAR_021355581Q → L.1 PublicationCorresponds to variant rs28399659dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83425 mRNA. Translation: CAA58449.1.
AY845133 Genomic DNA. Translation: AAV88096.1.
AC092306 Genomic DNA. No translation available.
CH471126 Genomic DNA. Translation: EAW57297.1. Sequence problems.
BC050450 mRNA. Translation: AAH50450.1.
X80026 mRNA. Translation: CAA56327.1.
EU307108 Genomic DNA. Translation: ABY27636.1.
EU307109 Genomic DNA. Translation: ABY27637.1.
CCDSiCCDS12644.1.
PIRiI37202.
I38000.
RefSeqiNP_001013275.1. NM_001013257.2.
NP_005572.2. NM_005581.4.
UniGeneiHs.625725.

Genome annotation databases

EnsembliENST00000270233; ENSP00000270233; ENSG00000187244.
GeneIDi4059.
KEGGihsa:4059.
UCSCiuc002ozu.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

dbRBC/BGMUT

Blood group antigen gene mutation database

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83425 mRNA. Translation: CAA58449.1.
AY845133 Genomic DNA. Translation: AAV88096.1.
AC092306 Genomic DNA. No translation available.
CH471126 Genomic DNA. Translation: EAW57297.1. Sequence problems.
BC050450 mRNA. Translation: AAH50450.1.
X80026 mRNA. Translation: CAA56327.1.
EU307108 Genomic DNA. Translation: ABY27636.1.
EU307109 Genomic DNA. Translation: ABY27637.1.
CCDSiCCDS12644.1.
PIRiI37202.
I38000.
RefSeqiNP_001013275.1. NM_001013257.2.
NP_005572.2. NM_005581.4.
UniGeneiHs.625725.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PETX-ray1.70A32-262[»]
2PF6X-ray2.20A/B32-262[»]
ProteinModelPortaliP50895.
SMRiP50895.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110237. 19 interactors.
IntActiP50895. 5 interactors.
MINTiMINT-5004331.
STRINGi9606.ENSP00000270233.

PTM databases

iPTMnetiP50895.
PhosphoSitePlusiP50895.
SwissPalmiP50895.

Polymorphism and mutation databases

BioMutaiBCAM.
DMDMi92058724.

Proteomic databases

EPDiP50895.
MaxQBiP50895.
PaxDbiP50895.
PeptideAtlasiP50895.
PRIDEiP50895.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000270233; ENSP00000270233; ENSG00000187244.
GeneIDi4059.
KEGGihsa:4059.
UCSCiuc002ozu.5. human.

Organism-specific databases

CTDi4059.
DisGeNETi4059.
GeneCardsiBCAM.
HGNCiHGNC:6722. BCAM.
HPAiHPA005654.
MIMi111200. phenotype.
247420. phenotype.
612773. gene.
neXtProtiNX_P50895.
OpenTargetsiENSG00000187244.
PharmGKBiPA30484.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJIS. Eukaryota.
ENOG4111XZT. LUCA.
GeneTreeiENSGT00530000063457.
HOGENOMiHOG000113409.
InParanoidiP50895.
KOiK06578.
OMAiWFLTDRS.
OrthoDBiEOG091G05IA.
PhylomeDBiP50895.
TreeFamiTF330534.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000142232-MONOMER.

Miscellaneous databases

ChiTaRSiBCAM. human.
EvolutionaryTraceiP50895.
GeneWikiiBCAM.
GenomeRNAii4059.
PROiP50895.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000187244.
CleanExiHS_BCAM.
ExpressionAtlasiP50895. baseline and differential.
GenevisibleiP50895. HS.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF13895. Ig_2. 2 hits.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 5 hits.
SM00408. IGc2. 3 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiPS50835. IG_LIKE. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCAM_HUMAN
AccessioniPrimary (citable) accession number: P50895
Secondary accession number(s): A8MYF9
, A9YWT5, A9YWT6, Q86VC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 7, 2006
Last modified: November 30, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Blood group antigen proteins
    Nomenclature of blood group antigens and list of entries
  2. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  8. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.