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Protein

40S ribosomal protein SA

Gene

RPSA

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis.UniRule annotation

GO - Molecular functioni

  • laminin binding Source: UniProtKB
  • laminin receptor activity Source: UniProtKB-HAMAP
  • structural constituent of ribosome Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor, Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

ReactomeiR-GGA-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-GGA-6791226. Major pathway of rRNA processing in the nucleolus.
R-GGA-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-GGA-72702. Ribosomal scanning and start codon recognition.
R-GGA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-GGA-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-GGA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein SAUniRule annotation
Alternative name(s):
37 kDa laminin receptor precursorUniRule annotation
Short name:
37LRPUniRule annotation
37/67 kDa laminin receptorUniRule annotation
Short name:
LRP/LRUniRule annotation
67 kDa laminin receptorUniRule annotation
Short name:
67LRUniRule annotation
Laminin receptor 1UniRule annotation
Short name:
LamRUniRule annotation
Laminin-binding protein precursor p40UniRule annotation
Short name:
LBP/p40UniRule annotation
Gene namesi
Name:RPSAUniRule annotation
Synonyms:LAMR1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 2

Subcellular locationi

  • Cell membrane UniRule annotation
  • Cytoplasm
  • Nucleus UniRule annotation

  • Note: 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedUniRule annotation
Chaini2 – 29629540S ribosomal protein SAPRO_0000134361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineUniRule annotation

Post-translational modificationi

Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.UniRule annotation
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei115 – 1162Cleavage; by ST3; site 1UniRule annotation
Sitei133 – 1342Cleavage; by ST3; site 2UniRule annotation

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP50890.
PRIDEiP50890.

Interactioni

Subunit structurei

Monomer (37LRP) and homodimer (67LR). Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Interacts with RPS21. Interacts with several laminins including at least LAMB1. Interacts with MDK.UniRule annotation

GO - Molecular functioni

  • laminin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi675476. 2 interactions.
IntActiP50890. 1 interaction.
STRINGi9031.ENSGALP00000019469.

Structurei

3D structure databases

ProteinModelPortaliP50890.
SMRiP50890. Positions 9-205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati230 – 2323[DE]-W-[ST] 1
Repeati248 – 2503[DE]-W-[ST] 2
Repeati267 – 2693[DE]-W-[ST] 3
Repeati276 – 2783[DE]-W-[ST] 4
Repeati294 – 2963[DE]-W-[ST] 5

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 18020Laminin-bindingUniRule annotationAdd
BLAST
Regioni205 – 22925Laminin-bindingUniRule annotationAdd
BLAST
Regioni243 – 29654Laminin-bindingUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ribosomal protein S2P family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0830. Eukaryota.
COG0052. LUCA.
GeneTreeiENSGT00390000015036.
HOGENOMiHOG000232073.
HOVERGENiHBG054466.
InParanoidiP50890.
KOiK02998.
OMAiHNKPYVY.
OrthoDBiEOG73NG4F.
PhylomeDBiP50890.
TreeFamiTF300100.

Family and domain databases

HAMAPiMF_03015. Ribosomal_S2_euk.
MF_03016. Ribosomal_S2_laminin_receptor.
InterProiIPR027504. 40S_ribosomal_SA.
IPR032281. 40S_SA_C.
IPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]
PANTHERiPTHR11489. PTHR11489. 1 hit.
PfamiPF16122. 40S_SA_C. 1 hit.
PF00318. Ribosomal_S2. 2 hits.
[Graphical view]
PRINTSiPR00395. RIBOSOMALS2.
SUPFAMiSSF52313. SSF52313. 1 hit.
TIGRFAMsiTIGR01012. uS2_euk_arch. 1 hit.
PROSITEiPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50890-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGLDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN
60 70 80 90 100
LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLKF AAATGATPIA
110 120 130 140 150
GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NIPTIALCNT
160 170 180 190 200
DSPLRYVDIA IPCNNKGAHS VGLMWWMLAR EVLRMRGTIS REHPWEVMPD
210 220 230 240 250
LYFYRDPEEI EKEEQAAAEK AVTKEEFQTE WTAPAPEFTA PPQPEVADWS
260 270 280 290
EGVQVPSVPI QQFPTEDWSA QPATEDWSAA PTAQATEWVG TTTEWS
Length:296
Mass (Da):33,021
Last modified:October 1, 1996 - v1
Checksum:i3486A86EF514E5CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94368 Genomic DNA. Translation: CAA64147.1.
RefSeqiNP_001007824.1. NM_001007823.1.
UniGeneiGga.34355.

Genome annotation databases

EnsembliENSGALT00000019495; ENSGALP00000019469; ENSGALG00000011951.
GeneIDi395181.
KEGGigga:395181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94368 Genomic DNA. Translation: CAA64147.1.
RefSeqiNP_001007824.1. NM_001007823.1.
UniGeneiGga.34355.

3D structure databases

ProteinModelPortaliP50890.
SMRiP50890. Positions 9-205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi675476. 2 interactions.
IntActiP50890. 1 interaction.
STRINGi9031.ENSGALP00000019469.

Proteomic databases

PaxDbiP50890.
PRIDEiP50890.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000019495; ENSGALP00000019469; ENSGALG00000011951.
GeneIDi395181.
KEGGigga:395181.

Organism-specific databases

CTDi3921.

Phylogenomic databases

eggNOGiKOG0830. Eukaryota.
COG0052. LUCA.
GeneTreeiENSGT00390000015036.
HOGENOMiHOG000232073.
HOVERGENiHBG054466.
InParanoidiP50890.
KOiK02998.
OMAiHNKPYVY.
OrthoDBiEOG73NG4F.
PhylomeDBiP50890.
TreeFamiTF300100.

Enzyme and pathway databases

ReactomeiR-GGA-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-GGA-6791226. Major pathway of rRNA processing in the nucleolus.
R-GGA-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-GGA-72702. Ribosomal scanning and start codon recognition.
R-GGA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-GGA-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-GGA-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiP50890.

Family and domain databases

HAMAPiMF_03015. Ribosomal_S2_euk.
MF_03016. Ribosomal_S2_laminin_receptor.
InterProiIPR027504. 40S_ribosomal_SA.
IPR032281. 40S_SA_C.
IPR001865. Ribosomal_S2.
IPR018130. Ribosomal_S2_CS.
IPR027498. Ribosomal_S2_euk.
IPR005707. Ribosomal_S2_euk/arc.
IPR023591. Ribosomal_S2_flav_dom.
[Graphical view]
PANTHERiPTHR11489. PTHR11489. 1 hit.
PfamiPF16122. 40S_SA_C. 1 hit.
PF00318. Ribosomal_S2. 2 hits.
[Graphical view]
PRINTSiPR00395. RIBOSOMALS2.
SUPFAMiSSF52313. SSF52313. 1 hit.
TIGRFAMsiTIGR01012. uS2_euk_arch. 1 hit.
PROSITEiPS00962. RIBOSOMAL_S2_1. 1 hit.
PS00963. RIBOSOMAL_S2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of the active gene coding for the metastasis-associated 37LRP/p40 multifunctional protein."
    Clausse N., Jackers P., Jares P., Joris B., Sobel M.E., Castronovo V.
    DNA Cell Biol. 15:1009-1023(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: White leghorn.
    Tissue: Spleen.

Entry informationi

Entry nameiRSSA_CHICK
AccessioniPrimary (citable) accession number: P50890
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein appears to have acquired a second function as a laminin receptor specifically in the vertebrate lineage.
It is thought that in vertebrates 37/67 kDa laminin receptor acquired a dual function during evolution. It developed from the ribosomal protein SA, playing an essential role in the protein biosynthesis lacking any laminin binding activity, to a cell surface receptor with laminin binding activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.