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Protein

E3 ubiquitin-protein ligase RNF144A

Gene

RNF144A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri20 – 7051RING-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri91 – 15666IBR-typeAdd
BLAST
Zinc fingeri185 – 21430RING-type 2; degeneratePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF144A (EC:6.3.2.-)
Alternative name(s):
RING finger protein 144A
UbcM4-interacting protein 4
Ubiquitin-conjugating enzyme 7-interacting protein 4
Gene namesi
Name:RNF144A
Synonyms:KIAA0161, RNF144, UBCE7IP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:20457. RNF144A.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei250 – 27021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162401542.

Polymorphism and mutation databases

BioMutaiRNF144A.
DMDMi160358924.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292E3 ubiquitin-protein ligase RNF144APRO_0000056298Add
BLAST

Post-translational modificationi

Autoubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP50876.
PRIDEiP50876.

Expressioni

Gene expression databases

BgeeiENSG00000151692.
CleanExiHS_RNF144A.
ExpressionAtlasiP50876. baseline and differential.
GenevisibleiP50876. HS.

Organism-specific databases

HPAiHPA049939.

Interactioni

Subunit structurei

Interacts with UBE2L3.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115125. 16 interactions.
IntActiP50876. 6 interactions.
STRINGi9606.ENSP00000321330.

Structurei

Secondary structure

1
292
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi21 – 233Combined sources
Helixi29 – 313Combined sources
Beta strandi32 – 354Combined sources
Turni36 – 394Combined sources
Beta strandi40 – 434Combined sources
Helixi44 – 5714Combined sources
Helixi78 – 847Combined sources
Helixi87 – 9913Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WIMNMR-A20-100[»]
ProteinModelPortaliP50876.
SMRiP50876. Positions 20-219.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50876.

Family & Domainsi

Sequence similaritiesi

Belongs to the RBR family. RNF144 subfamily.Curated
Contains 1 IBR-type zinc finger.Curated
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri20 – 7051RING-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri91 – 15666IBR-typeAdd
BLAST
Zinc fingeri185 – 21430RING-type 2; degeneratePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129738.
HOGENOMiHOG000007696.
HOVERGENiHBG052072.
InParanoidiP50876.
KOiK11975.
OMAiRWHPGQG.
OrthoDBiEOG091G0FQQ.
PhylomeDBiP50876.
TreeFamiTF324777.

Family and domain databases

InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTRYRPTW DLALDPLVSC KLCLGEYPVE QMTTIAQCQC IFCTLCLKQY
60 70 80 90 100
VELLIKEGLE TAISCPDAAC PKQGHLQENE IECMVAAEIM QRYKKLQFER
110 120 130 140 150
EVLFDPCRTW CPASTCQAVC QLQDVGLQTP QPVQCKACRM EFCSTCKASW
160 170 180 190 200
HPGQGCPETM PITFLPGETS AAFKMEEDDA PIKRCPKCKV YIERDEGCAQ
210 220 230 240 250
MMCKNCKHAF CWYCLESLDD DFLLIHYDKG PCRNKLGHSR ASVIWHRTQV
260 270 280 290
VGIFAGFGLL LLVASPFLLL ATPFVLCCKC KCSKGDDDPL PT
Length:292
Mass (Da):32,890
Last modified:October 23, 2007 - v2
Checksum:iE5BE7BC9560DCF93
GO

Sequence cautioni

The sequence BAA11478 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti4 – 41T → A.3 Publications
Corresponds to variant rs364891 [ dbSNP | Ensembl ].
VAR_035375

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79983 mRNA. Translation: BAA11478.2. Different initiation.
AC068481 Genomic DNA. Translation: AAX82010.1.
CH471053 Genomic DNA. Translation: EAX01030.1.
CH471053 Genomic DNA. Translation: EAX01031.1.
BC050373 mRNA. Translation: AAH50373.1.
CCDSiCCDS1657.1.
RefSeqiNP_055561.2. NM_014746.4.
XP_005246257.1. XM_005246200.3.
XP_005246259.1. XM_005246202.4.
UniGeneiHs.22146.

Genome annotation databases

EnsembliENST00000320892; ENSP00000321330; ENSG00000151692.
GeneIDi9781.
KEGGihsa:9781.
UCSCiuc002qys.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79983 mRNA. Translation: BAA11478.2. Different initiation.
AC068481 Genomic DNA. Translation: AAX82010.1.
CH471053 Genomic DNA. Translation: EAX01030.1.
CH471053 Genomic DNA. Translation: EAX01031.1.
BC050373 mRNA. Translation: AAH50373.1.
CCDSiCCDS1657.1.
RefSeqiNP_055561.2. NM_014746.4.
XP_005246257.1. XM_005246200.3.
XP_005246259.1. XM_005246202.4.
UniGeneiHs.22146.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WIMNMR-A20-100[»]
ProteinModelPortaliP50876.
SMRiP50876. Positions 20-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115125. 16 interactions.
IntActiP50876. 6 interactions.
STRINGi9606.ENSP00000321330.

Polymorphism and mutation databases

BioMutaiRNF144A.
DMDMi160358924.

Proteomic databases

PaxDbiP50876.
PRIDEiP50876.

Protocols and materials databases

DNASUi9781.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000320892; ENSP00000321330; ENSG00000151692.
GeneIDi9781.
KEGGihsa:9781.
UCSCiuc002qys.4. human.

Organism-specific databases

CTDi9781.
GeneCardsiRNF144A.
HGNCiHGNC:20457. RNF144A.
HPAiHPA049939.
neXtProtiNX_P50876.
PharmGKBiPA162401542.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129738.
HOGENOMiHOG000007696.
HOVERGENiHBG052072.
InParanoidiP50876.
KOiK11975.
OMAiRWHPGQG.
OrthoDBiEOG091G0FQQ.
PhylomeDBiP50876.
TreeFamiTF324777.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiRNF144A. human.
EvolutionaryTraceiP50876.
GenomeRNAii9781.
PROiP50876.

Gene expression databases

BgeeiENSG00000151692.
CleanExiHS_RNF144A.
ExpressionAtlasiP50876. baseline and differential.
GenevisibleiP50876. HS.

Family and domain databases

InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiR144A_HUMAN
AccessioniPrimary (citable) accession number: P50876
Secondary accession number(s): D6W4Y6, Q585H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 23, 2007
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.