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Protein

E3 ubiquitin-protein ligase RNF144A

Gene

RNF144A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.By similarity1 Publication

Caution

Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.Curated

Catalytic activityi

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei198By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri20 – 70RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri91 – 156IBR-typeAdd BLAST66
Zinc fingeri185 – 214RING-type 2; degeneratePROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF144A (EC:2.3.2.31By similarity)
Alternative name(s):
RING finger protein 144A
UbcM4-interacting protein 4
Ubiquitin-conjugating enzyme 7-interacting protein 4
Gene namesi
Name:RNF144A
Synonyms:KIAA0161, RNF144, UBCE7IP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000151692.14
HGNCiHGNC:20457 RNF144A
neXtProtiNX_P50876

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei250 – 270HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9781
OpenTargetsiENSG00000151692
PharmGKBiPA162401542

Polymorphism and mutation databases

BioMutaiRNF144A
DMDMi160358924

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562981 – 292E3 ubiquitin-protein ligase RNF144AAdd BLAST292

Post-translational modificationi

Autoubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP50876
PeptideAtlasiP50876
PRIDEiP50876

Expressioni

Gene expression databases

BgeeiENSG00000151692
CleanExiHS_RNF144A
ExpressionAtlasiP50876 baseline and differential
GenevisibleiP50876 HS

Organism-specific databases

HPAiHPA049939

Interactioni

Subunit structurei

Interacts with UBE2L3.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115125, 16 interactors
IntActiP50876, 8 interactors
STRINGi9606.ENSP00000321330

Structurei

Secondary structure

1292
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 23Combined sources3
Helixi29 – 31Combined sources3
Beta strandi32 – 35Combined sources4
Turni36 – 39Combined sources4
Beta strandi40 – 43Combined sources4
Helixi44 – 57Combined sources14
Helixi78 – 84Combined sources7
Helixi87 – 99Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WIMNMR-A20-100[»]
ProteinModelPortaliP50876
SMRiP50876
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50876

Family & Domainsi

Domaini

Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.By similarity

Sequence similaritiesi

Belongs to the RBR family. RNF144 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri20 – 70RING-type 1; atypicalPROSITE-ProRule annotationAdd BLAST51
Zinc fingeri91 – 156IBR-typeAdd BLAST66
Zinc fingeri185 – 214RING-type 2; degeneratePROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815 Eukaryota
ENOG410XP9Y LUCA
GeneTreeiENSGT00840000129738
HOGENOMiHOG000007696
HOVERGENiHBG052072
InParanoidiP50876
KOiK11975
OMAiPQLVQCK
OrthoDBiEOG091G0FQQ
PhylomeDBiP50876
TreeFamiTF324777

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031127 E3_UB_ligase_RBR
IPR002867 IBR_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR11685 PTHR11685, 1 hit
PfamiView protein in Pfam
PF01485 IBR, 2 hits
SMARTiView protein in SMART
SM00647 IBR, 2 hits
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

P50876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTRYRPTW DLALDPLVSC KLCLGEYPVE QMTTIAQCQC IFCTLCLKQY
60 70 80 90 100
VELLIKEGLE TAISCPDAAC PKQGHLQENE IECMVAAEIM QRYKKLQFER
110 120 130 140 150
EVLFDPCRTW CPASTCQAVC QLQDVGLQTP QPVQCKACRM EFCSTCKASW
160 170 180 190 200
HPGQGCPETM PITFLPGETS AAFKMEEDDA PIKRCPKCKV YIERDEGCAQ
210 220 230 240 250
MMCKNCKHAF CWYCLESLDD DFLLIHYDKG PCRNKLGHSR ASVIWHRTQV
260 270 280 290
VGIFAGFGLL LLVASPFLLL ATPFVLCCKC KCSKGDDDPL PT
Length:292
Mass (Da):32,890
Last modified:October 23, 2007 - v2
Checksum:iE5BE7BC9560DCF93
GO

Sequence cautioni

The sequence BAA11478 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0353754T → A3 PublicationsCorresponds to variant dbSNP:rs364891Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D79983 mRNA Translation: BAA11478.2 Different initiation.
AC068481 Genomic DNA Translation: AAX82010.1
CH471053 Genomic DNA Translation: EAX01030.1
CH471053 Genomic DNA Translation: EAX01031.1
BC050373 mRNA Translation: AAH50373.1
CCDSiCCDS1657.1
RefSeqiNP_055561.2, NM_014746.4
XP_005246257.1, XM_005246200.3
XP_005246259.1, XM_005246202.4
XP_016860885.1, XM_017005396.1
XP_016860886.1, XM_017005397.1
UniGeneiHs.22146
Hs.716039

Genome annotation databases

EnsembliENST00000320892; ENSP00000321330; ENSG00000151692
GeneIDi9781
KEGGihsa:9781
UCSCiuc002qys.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiR144A_HUMAN
AccessioniPrimary (citable) accession number: P50876
Secondary accession number(s): D6W4Y6, Q585H5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 23, 2007
Last modified: April 25, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

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