Probable E3 ubiquitin-protein ligase RNF144A

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Reviewed, UniProtKB/Swiss-Prot P50876 (R144A_HUMAN)

Last modified June 16, 2009. Version 78. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Probable E3 ubiquitin-protein ligase RNF144A
    EC=6.3.2.-
Alternative name(s):
    RING finger protein 144A
    Ubiquitin-conjugating enzyme 7-interacting protein 4
    UbcM4-interacting protein 4

Gene names

Name:	RNF144A
Synonyms:	KIAA0161, RNF144, UBCE7IP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with UBE2L3. Ref.4
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the RBR family. RNF144 subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
Caution	Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.

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Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 292

292

Probable E3 ubiquitin-protein ligase RNF144A

PRO_0000056298

Regions

Transmembrane

250 – 270

Potential

Zinc finger

20 – 70

RING-type 1; atypical

Zinc finger

91 – 156

IBR-type

Zinc finger

185 – 214

RING-type 2; degenerate

Natural variations

Natural variant

T → A: dbSNP rs364891. Ref.1 Ref.3

VAR_035375

Secondary structure

292

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P50876-1 [UniParc].

Last modified October 23, 2007. Version 2.
Checksum: E5BE7BC9560DCF93
Show »

FASTA

292

32,890

        10         20         30         40         50         60 
MTTTRYRPTW DLALDPLVSC KLCLGEYPVE QMTTIAQCQC IFCTLCLKQY VELLIKEGLE 

        70         80         90        100        110        120 
TAISCPDAAC PKQGHLQENE IECMVAAEIM QRYKKLQFER EVLFDPCRTW CPASTCQAVC 

       130        140        150        160        170        180 
QLQDVGLQTP QPVQCKACRM EFCSTCKASW HPGQGCPETM PITFLPGETS AAFKMEEDDA 

       190        200        210        220        230        240 
PIKRCPKCKV YIERDEGCAQ MMCKNCKHAF CWYCLESLDD DFLLIHYDKG PCRNKLGHSR 

       250        260        270        280        290 
ASVIWHRTQV VGIFAGFGLL LLVASPFLLL ATPFVLCCKC KCSKGDDDPL PT

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N. DNA Res. 3:17-24(1996) [PubMed: 8724849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-4. Tissue: Bone marrow.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-4. Tissue: Ovary.
[4]	"A family of structurally related RING finger proteins interacts specifically with the ubiquitin-conjugating enzyme UbcM4." Martinez-Noel G., Niedenthal R., Tamura T., Harbers K. FEBS Lett. 454:257-261(1999) [PubMed: 10431818] [Abstract] Cited for: INTERACTION WITH UBE2L3.
[5]	"Solution structure of the ring finger domain of the human UbcM4-interacting protein 4." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 20-100.

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Cross-references

Sequence databases

D79983 mRNA. Translation: BAA11478.2. Different initiation.
AC068481 Genomic DNA. Translation: AAX82010.1.
BC050373 mRNA. Translation: AAH50373.1.

IPI

IPI00220842.

RefSeq

NP_055561.2.

UniGene

Hs.699230

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WIM	NMR	-	A	20-100	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSG00000151692. Homo sapiens. [Contig view]

GeneID

9781.

KEGG

hsa:9781.

Organism-specific databases

GeneCards

GC02P006974.

H-InvDB

HIX0001799.

HGNC

HGNC:20457. RNF144A.

PharmGKB

PA134900632.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P50876.

HOVERGEN

P50876.

OMA

P50876. ASTCQAV.

Gene expression databases

ArrayExpress

P50876.

Bgee

P50876.

CleanEx

HS_RNF144A.

Family and domain databases

InterPro

IPR002867. Znf_C6HC.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]

Pfam

PF01485. IBR. 1 hit.
[Graphical view]

SMART

SM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]

PROSITE

PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

36828.

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Entry information

Entry name

R144A_HUMAN

Accession

Primary (citable) accession number: P50876
Secondary accession number(s): Q585H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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