P50876 (R144A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable E3 ubiquitin-protein ligase RNF144A
EC=6.3.2.-

Alternative name(s):
RING finger protein 144A
UbcM4-interacting protein 4
Ubiquitin-conjugating enzyme 7-interacting protein 4

Gene names

Name:	RNF144A
Synonyms:	KIAA0161, RNF144, UBCE7IP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with UBE2L3. Ref.5
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the RBR family. RNF144 subfamily. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
Caution	Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.
Sequence caution	The sequence BAA11478.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 292

292

Probable E3 ubiquitin-protein ligase RNF144A

PRO_0000056298

Regions

Transmembrane

250 – 270

21

Helical; Potential

Zinc finger

20 – 70

51

RING-type 1; atypical

Zinc finger

91 – 156

66

IBR-type

Zinc finger

185 – 214

30

RING-type 2; degenerate

Natural variations

Natural variant

4

1

T → A. Ref.1 Ref.3 Ref.4
Corresponds to variant rs364891 [ dbSNP | Ensembl ].

VAR_035375

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

292

Helix Strand Turn

Details...

Beta strand

21 – 23

3

Helix

29 – 31

3

Beta strand

32 – 35

4

Turn

36 – 39

4

Beta strand

40 – 43

4

Helix

44 – 57

14

Helix

78 – 84

7

Helix

87 – 99

13

Sequences

	Sequence		Length	Mass (Da)
	P50876 [UniParc]. Last modified October 23, 2007. Version 2. Checksum: E5BE7BC9560DCF93	FASTA	292	32,890
	10 20 30 40 50 60 MTTTRYRPTW DLALDPLVSC KLCLGEYPVE QMTTIAQCQC IFCTLCLKQY VELLIKEGLE 70 80 90 100 110 120 TAISCPDAAC PKQGHLQENE IECMVAAEIM QRYKKLQFER EVLFDPCRTW CPASTCQAVC 130 140 150 160 170 180 QLQDVGLQTP QPVQCKACRM EFCSTCKASW HPGQGCPETM PITFLPGETS AAFKMEEDDA 190 200 210 220 230 240 PIKRCPKCKV YIERDEGCAQ MMCKNCKHAF CWYCLESLDD DFLLIHYDKG PCRNKLGHSR 250 260 270 280 290 ASVIWHRTQV VGIFAGFGLL LLVASPFLLL ATPFVLCCKC KCSKGDDDPL PT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N. DNA Res. 3:17-24(1996) [PubMed: 8724849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-4. Tissue: Bone marrow.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-4.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-4. Tissue: Ovary.
[5]	"A family of structurally related RING finger proteins interacts specifically with the ubiquitin-conjugating enzyme UbcM4." Martinez-Noel G., Niedenthal R., Tamura T., Harbers K. FEBS Lett. 454:257-261(1999) [PubMed: 10431818] [Abstract] Cited for: INTERACTION WITH UBE2L3.
[6]	"Solution structure of the RING finger domain of the human UbcM4-interacting protein 4." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 20-100.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D79983 mRNA. Translation: BAA11478.2. Different initiation.
AC068481 Genomic DNA. Translation: AAX82010.1.
CH471053 Genomic DNA. Translation: EAX01030.1.
CH471053 Genomic DNA. Translation: EAX01031.1.
BC050373 mRNA. Translation: AAH50373.1.

IPI

IPI00220842.

RefSeq

NP_055561.2. NM_014746.3.

UniGene

Hs.22146.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WIM	NMR	-	A	20-100	[»]

ProteinModelPortal

P50876.

SMR

P50876. Positions 20-157, 179-230.

ModBase

Search...

Protein-protein interaction databases

IntAct

P50876. 6 interactions.

STRING

P50876.

Polymorphism databases

DMDM

160358924.

Proteomic databases

PRIDE

P50876.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000320892; ENSP00000321330; ENSG00000151692.

GeneID

9781.

KEGG

hsa:9781.

UCSC

uc002qys.1. human.

Organism-specific databases

CTD

9781.

GeneCards

GC02P007057.

HGNC

HGNC:20457. RNF144A.

neXtProt

NX_P50876.

PharmGKB

PA162401542.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG07763.

GeneTree

ENSGT00550000074424.

HOGENOM

HBG314219.

HOVERGEN

HBG052072.

InParanoid

P50876.

OMA

CKCSKGD.

OrthoDB

EOG4WWRK1.

PhylomeDB

P50876.

Gene expression databases

ArrayExpress

P50876.

Bgee

P50876.

CleanEx

HS_RNF144A.

Genevestigator

P50876.

Family and domain databases

InterPro

IPR002867. Znf_C6HC.
IPR001841. Znf_RING.
IPR017907. Znf_RING_CS.
[Graphical view]

KO

K11975.

Pfam

PF01485. IBR. 2 hits.
[Graphical view]

SMART

SM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]

PROSITE

PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

36828.

Entry information

Entry name

R144A_HUMAN

Accession

Primary (citable) accession number: P50876
Secondary accession number(s): D6W4Y6, Q585H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 23, 2007
Last modified:	January 25, 2012
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.