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Protein

Transcription factor SPT20

Gene

SPT20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription regulator. May recruit TATA binding protein (TBP) and possibly other basal factors to bind to the TATA box. Functions as component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.1 Publication

Miscellaneous

Present with 4150 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • transcription cofactor activity Source: SGD

GO - Biological processi

  • chromatin organization Source: SGD
  • histone acetylation Source: SGD
  • mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33538-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor SPT20
Gene namesi
Name:SPT20
Synonyms:ADA5
Ordered Locus Names:YOL148C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL148C.
SGDiS000005508. SPT20.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721611 – 604Transcription factor SPT20Add BLAST604

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei446PhosphoserineCombined sources1
Modified residuei516PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP50875.
PaxDbiP50875.
PRIDEiP50875.

PTM databases

iPTMnetiP50875.

Interactioni

Subunit structurei

Component of the 1.8 MDa SAGA complex, which consists of at least of TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA. SUS1 associates with the SAC3-THP1 complex. Component of the SALSA complex, which consists of at least TRA1, SPT7 (C-terminal truncated form), TAF5, ADA3, SPT20, TAF12, TAF6, HFI1, GCN5, ADA2 and SPT3. Component of the SLIK complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SPT20, RTG2, TAF12, TAF6, HFI1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10 and TAF9. Component of the ADA/GCN5 complex that consists of HFI1/ADA1, ADA2, ADA3, SPT20/ADA5 and GCN5 and is probably a subcomplex of SAGA.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34269. 89 interactors.
DIPiDIP-933N.
IntActiP50875. 93 interactors.
MINTiP50875.
STRINGi4932.YOL148C.

Structurei

3D structure databases

ProteinModelPortaliP50875.
SMRiP50875.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi157 – 162Poly-Gln6
Compositional biasi235 – 240Poly-Ser6
Compositional biasi422 – 425Poly-Ser4
Compositional biasi454 – 463Poly-Ala10
Compositional biasi552 – 559Poly-Asn8

Sequence similaritiesi

Belongs to the SPT20 family.Curated

Phylogenomic databases

HOGENOMiHOG000141872.
InParanoidiP50875.
KOiK11361.
OMAiWRINKEK.
OrthoDBiEOG092C3M2G.

Family and domain databases

InterProiView protein in InterPro
IPR021950. Spt20.
PfamiView protein in Pfam
PF12090. Spt20. 1 hit.

Sequencei

Sequence statusi: Complete.

P50875-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANSPTGND PHVFGIPVNA TPSNMGSPGS PVNVPPPMNP AVANVNHPVM
60 70 80 90 100
RTNSNSNANE GTRTLTREQI QQLQQRQRLL LQQRLLEQQR KQQALQNYEA
110 120 130 140 150
QFYQMLMTLN KRPKRLYNFV EDADSILKKY EQYLHSFEFH IYENNYKICA
160 170 180 190 200
PANSRLQQQQ KQPELTSDGL ILTKNNETLK EFLEYVARGR IPDAIMEVLR
210 220 230 240 250
DCNIQFYEGN LILQVYDHTN TVDVTPKENK PNLNSSSSPS NNNSTQDNSK
260 270 280 290 300
IQQPSEPNSG VANTGANTAN KKASFKRPRV YRTLLKPNDL TTYYDMMSYA
310 320 330 340 350
DNARFSDSIY QQFESEILTL TKRNLSLSVP LNPYEHRDML EETAFSEPHW
360 370 380 390 400
DSEKKSFIHE HRAESTREGT KGVVGHIEER DEFPQHSSNY EQLMLIMNER
410 420 430 440 450
TTTITNSTFA VSLTKNAMEI ASSSSNGVRG ASSSTSNSAS NTRNNSLANG
460 470 480 490 500
NQVALAAAAA AAAVGSTMGN DNNQFSRLKF IEQWRINKEK RKQQALSANI
510 520 530 540 550
NPTPFNARIS MTAPLTPQQQ LLQRQQQALE QQQNGGAMKN ANKRSGNNAT
560 570 580 590 600
SNNNNNNNNL DKPKVKRPRK NAKKSESGTP APKKKRMTKK KQSASSTPSS

TTMS
Length:604
Mass (Da):67,796
Last modified:October 1, 1996 - v1
Checksum:i3D67937B65F9AA6A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti293Y → S in CAA88279 (PubMed:8553699).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43153 Genomic DNA. Translation: AAB07899.1.
U22063 Genomic DNA. Translation: AAB07900.1.
Z74890 Genomic DNA. Translation: CAA99169.1.
Z48239 Genomic DNA. Translation: CAA88279.1.
BK006948 Genomic DNA. Translation: DAA10637.1.
PIRiS58643.
RefSeqiNP_014493.1. NM_001183402.1.

Genome annotation databases

EnsemblFungiiYOL148C; YOL148C; YOL148C.
GeneIDi854017.
KEGGisce:YOL148C.

Similar proteinsi

Entry informationi

Entry nameiSPT20_YEAST
AccessioniPrimary (citable) accession number: P50875
Secondary accession number(s): D6W1S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome