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Protein

Streptogramin A acetyltransferase

Gene

vatD

Organism
Enterococcus faecium (Streptococcus faecium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei82 – 821

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Streptogramin A acetyltransferase (EC:2.3.1.-)
Alternative name(s):
Virginiamycin acetyltransferase D
Short name:
Vat(D)
Gene namesi
Name:vatD
Synonyms:satA
OrganismiEnterococcus faecium (Streptococcus faecium)
Taxonomic identifieri1352 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi82 – 821H → A: 105-fold decrease in activity. 1 Publication

Chemistry

DrugBankiDB01764. Dalfopristin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 209209Streptogramin A acetyltransferasePRO_0000068663Add
BLAST

Interactioni

Subunit structurei

Homohexamer.

Structurei

Secondary structure

1
209
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 133Combined sources
Beta strandi16 – 194Combined sources
Helixi20 – 245Combined sources
Beta strandi30 – 323Combined sources
Beta strandi37 – 393Combined sources
Beta strandi41 – 433Combined sources
Helixi46 – 494Combined sources
Beta strandi50 – 523Combined sources
Helixi55 – 573Combined sources
Beta strandi61 – 633Combined sources
Beta strandi74 – 763Combined sources
Helixi78 – 803Combined sources
Helixi91 – 944Combined sources
Helixi98 – 1014Combined sources
Helixi105 – 1073Combined sources
Beta strandi114 – 1163Combined sources
Beta strandi154 – 1574Combined sources
Turni158 – 1614Combined sources
Beta strandi162 – 1687Combined sources
Helixi170 – 17910Combined sources
Helixi181 – 1833Combined sources
Helixi186 – 1916Combined sources
Helixi193 – 1986Combined sources
Helixi200 – 2023Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KHRX-ray2.80A/B/C/D/E/F1-209[»]
1KK4X-ray2.70A/B/C/D/E/F1-209[»]
1KK5X-ray2.70A/B/C/D/E/F1-209[»]
1KK6X-ray2.50A/B/C1-209[»]
1MR7X-ray1.80A/B/C/X/Y/Z1-209[»]
1MR9X-ray3.00A/B/C/X/Y/Z1-209[»]
1MRLX-ray2.80A/B/C1-209[»]
3DHOX-ray1.80A/B/C/D/E/F1-209[»]
ProteinModelPortaliP50870.
SMRiP50870. Positions 1-207.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50870.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK18234.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50870-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPNPMKMYP IEGNKSVQFI KPILEKLENV EVGEYSYYDS KNGETFDKQI
60 70 80 90 100
LYHYPILNDK LKIGKFCSIG PGVTIIMNGA NHRMDGSTYP FNLFGNGWEK
110 120 130 140 150
HMPKLDQLPI KGDTIIGNDV WIGKDVVIMP GVKIGDGAIV AANSVVVKDI
160 170 180 190 200
APYMLAGGNP ANEIKQRFDQ DTINQLLDIK WWNWPIDIIN ENIDKILDNS

IIREVIWKK
Length:209
Mass (Da):23,649
Last modified:October 1, 1996 - v1
Checksum:i75F52F1DD0C135C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12033 Genomic DNA. Translation: AAA24783.1.

Genome annotation databases

KEGGiag:AAA24783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12033 Genomic DNA. Translation: AAA24783.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KHRX-ray2.80A/B/C/D/E/F1-209[»]
1KK4X-ray2.70A/B/C/D/E/F1-209[»]
1KK5X-ray2.70A/B/C/D/E/F1-209[»]
1KK6X-ray2.50A/B/C1-209[»]
1MR7X-ray1.80A/B/C/X/Y/Z1-209[»]
1MR9X-ray3.00A/B/C/X/Y/Z1-209[»]
1MRLX-ray2.80A/B/C1-209[»]
3DHOX-ray1.80A/B/C/D/E/F1-209[»]
ProteinModelPortaliP50870.
SMRiP50870. Positions 1-207.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

DrugBankiDB01764. Dalfopristin.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA24783.

Phylogenomic databases

KOiK18234.

Miscellaneous databases

EvolutionaryTraceiP50870.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of the satA gene encoding a streptogramin A acetyltransferase in Enterococcus faecium BM4145."
    Rende-Fournier R., Leclercq R., Galimand M., Duval J., Courvalin P.
    Antimicrob. Agents Chemother. 37:2119-2125(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BM4145.
  2. "Crystal structure of Vat(D): an acetyltransferase that inactivates streptogramin group A antibiotics."
    Sugantino M., Roderick S.L.
    Biochemistry 41:2209-2216(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
  3. "Structural basis of Synercid (quinupristin-dalfopristin) resistance in Gram-positive bacterial pathogens."
    Kehoe L.E., Snidwongse J., Courvalin P., Rafferty J.B., Murray I.A.
    J. Biol. Chem. 278:29963-29970(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), MUTAGENESIS OF HIS-82.

Entry informationi

Entry nameiVATD_ENTFC
AccessioniPrimary (citable) accession number: P50870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 11, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.