P50870 (VATD_ENTFC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Streptogramin A acetyltransferase
EC=2.3.1.-

Alternative name(s):
Virginiamycin acetyltransferase D
Short name=Vat(D)

Gene names

Name:	vatD
Synonyms:	satA

Organism

Enterococcus faecium (Streptococcus faecium)

Taxonomic identifier

1352 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 209

209

Streptogramin A acetyltransferase

PRO_0000068663

Sites

Active site

82

1

Experimental info

Mutagenesis

82

1

H → A: 105-fold decrease in activity. Ref.3

Secondary structure

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209

Helix Strand Turn

Details...

Beta strand

11 – 13

3

Beta strand

15 – 19

5

Helix

20 – 25

6

Beta strand

30 – 32

3

Beta strand

37 – 39

3

Beta strand

41 – 43

3

Helix

46 – 49

4

Beta strand

50 – 52

3

Helix

55 – 57

3

Beta strand

61 – 63

3

Beta strand

74 – 76

3

Helix

78 – 80

3

Helix

91 – 94

4

Helix

98 – 101

4

Helix

105 – 107

3

Beta strand

114 – 116

3

Beta strand

154 – 157

4

Turn

158 – 161

4

Beta strand

162 – 168

7

Helix

170 – 179

10

Helix

181 – 183

3

Helix

186 – 189

4

Turn

190 – 192

3

Helix

193 – 197

5

Helix

201 – 205

5

Sequences

	Sequence		Length	Mass (Da)
	P50870 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 75F52F1DD0C135C1	FASTA	209	23,649
	10 20 30 40 50 60 MGPNPMKMYP IEGNKSVQFI KPILEKLENV EVGEYSYYDS KNGETFDKQI LYHYPILNDK 70 80 90 100 110 120 LKIGKFCSIG PGVTIIMNGA NHRMDGSTYP FNLFGNGWEK HMPKLDQLPI KGDTIIGNDV 130 140 150 160 170 180 WIGKDVVIMP GVKIGDGAIV AANSVVVKDI APYMLAGGNP ANEIKQRFDQ DTINQLLDIK 190 200 WWNWPIDIIN ENIDKILDNS IIREVIWKK

References

[1]	"Identification of the satA gene encoding a streptogramin A acetyltransferase in Enterococcus faecium BM4145." Rende-Fournier R., Leclercq R., Galimand M., Duval J., Courvalin P. Antimicrob. Agents Chemother. 37:2119-2125(1993) [PubMed: 8257133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BM4145.
[2]	"Crystal structure of Vat(D): an acetyltransferase that inactivates streptogramin group A antibiotics." Sugantino M., Roderick S.L. Biochemistry 41:2209-2216(2002) [PubMed: 11841212] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
[3]	"Structural basis of Synercid (quinupristin-dalfopristin) resistance in Gram-positive bacterial pathogens." Kehoe L.E., Snidwongse J., Courvalin P., Rafferty J.B., Murray I.A. J. Biol. Chem. 278:29963-29970(2003) [PubMed: 12771141] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), MUTAGENESIS OF HIS-82.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L12033 Genomic DNA. Translation: AAA24783.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KHR	X-ray	2.80	A/B/C/D/E/F	1-209	[»]
1KK4	X-ray	2.70	A/B/C/D/E/F	1-209	[»]
1KK5	X-ray	2.70	A/B/C/D/E/F	1-209	[»]
1KK6	X-ray	2.50	A/B/C	1-209	[»]
1MR7	X-ray	1.80	A/B/C/X/Y/Z	1-209	[»]
1MR9	X-ray	3.00	A/B/C/X/Y/Z	1-209	[»]
1MRL	X-ray	2.80	A/B/C	1-209	[»]
3DHO	X-ray	1.80	A/B/C/D/E/F	1-209	[»]

ProteinModelPortal

P50870.

SMR

P50870. Positions 1-207.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR011004. Trimer_LpxA-like.
[Graphical view]

Pfam

PF00132. Hexapep. 1 hit.
[Graphical view]

SUPFAM

SSF51161. Trimer_LpxA_like. 1 hit.

PROSITE

PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB01764. Dalfopristin.

Entry information

Entry name

VATD_ENTFC

Accession

Primary (citable) accession number: P50870

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 19, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program