P50867 (CYSK_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase
Short name=OAS-TL
O-acetylserine sulfhydrylase

Gene names

Name:	cysB
ORF Names:	AN8057

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Cysteine synthase		PRO_0000167127
Regions
	Region	209 – 213	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	103	1	Pyridoxal phosphate By similarity
	Binding site	308	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	73	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	38	1	E → EK in AAC06128. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50867 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: 5672B5C43656C30A	FASTA	370	39,807
	10 20 30 40 50 60 MFRQSVRRFA TAALRSAAES PYNVRVSQAQ GFVNGLTEAI GNTPLIRLKR LSEETGSNIL 70 80 90 100 110 120 AKAEFQNPGG SVKDRAALYV VKDAEERGLL KPGGTVVEGT AGNTGIGLAH VCRSKGYKLV 130 140 150 160 170 180 IYMPNTQSQG KIDLLRLLGA EVYPVPAVAF DNPENYNHKA RRHAESLDNA VWTNQFDNTA 190 200 210 220 230 240 NRRAHIETTG PEIWAQTGGK LDAFTCSTGT GGTLAGITYY LKQASGGRVK SFLADPPGSV 250 260 270 280 290 300 LHSYIQSGGK LVERSGSSIT EGIGQGRITD NLQPDVGTLD GSLNISDEKT IEMIYRCLDE 310 320 330 340 350 360 EGLYLGASSA LNVVAAKEVA EKLGKGSTVV TILADGAYRY ADRLFSKSWL ESKGLRNAIP 370 KHLEKYIVLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Aspergillus nidulans cysB gene encoding cysteine synthase." Topczewski J., Sienko M., Paszewski A. Curr. Genet. 31:348-356(1997) [PubMed: 9108143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ANA1 BIA1 PHENA2.
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed: 19146970] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19395 Genomic DNA. Translation: AAC06128.1. AACD01000139 Genomic DNA. Translation: EAA59679.1. BN001302 Genomic DNA. Translation: CBF73799.1.
RefSeq	XP_681326.1. XM_676234.1.
3D structure databases
ProteinModelPortal	P50867.
ModBase	Search...
Protein-protein interaction databases
STRING	P50867.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANIAT00004083; CADANIAP00004083; CADANIAG00004083.
GeneID	2869229.
KEGG	ani:AN8057.2.
Phylogenomic databases
OMA	HVCRSKG.
OrthoDB	EOG4SFDFK.
PhylomeDB	P50867.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
KO	K01738.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_EMENI

Accession

Primary (citable) accession number: P50867
Secondary accession number(s): C8V672, Q5AUH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program