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Protein

Germination-specific N-acetylmuramoyl-L-alanine amidase

Gene

cwlD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the peptide side chain from the N-acetylmuramic acid residues in peptidoglycan. This is a step in the formation of muramic delta-lactam residues in spore cortex.1 Publication

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciBSUB:BSU01530-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Germination-specific N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Alternative name(s):
Autolysin
Cell wall hydrolase
Gene namesi
Name:cwlD
Ordered Locus Names:BSU01530
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU01530. [Micado]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 237210Germination-specific N-acetylmuramoyl-L-alanine amidasePRO_0000164419Add
BLAST

Proteomic databases

PaxDbiP50864.

Expressioni

Developmental stagei

Expression of cwlD takes place in both the mother cell and forespore compartments of sporulating cells.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU01530.

Structurei

3D structure databases

ProteinModelPortaliP50864.
SMRiP50864. Positions 41-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0860.
HOGENOMiHOG000163501.
InParanoidiP50864.
KOiK01448.
OMAiKCDAFIS.
OrthoDBiEOG6CP3X3.
PhylomeDBiP50864.

Family and domain databases

Gene3Di3.40.630.40. 1 hit.
InterProiIPR002508. CW_Hdrlase/autolysin_cat.
IPR014234. Spore_CwlD.
[Graphical view]
PfamiPF01520. Amidase_3. 1 hit.
[Graphical view]
SMARTiSM00646. Ami_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02883. spore_cwlD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50864-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKKLKWLSF LLGFIILLFL FKYQFSNNDS WKPWSLPLSG KIIYLDPGHG
60 70 80 90 100
GPDGGAVGGK LLEKDVTLEV AFRVRDYLQE QGALVIMTRE SDTDLAPEGT
110 120 130 140 150
KGYSRRKAED LRQRVKLINH SEAELYISIH LNAIPSQKWS GAQSFYYGKY
160 170 180 190 200
AENEKVAKYI QDELRRNLEN TTRKAKRIHG IYLMQNVTKP GALIEVGFLS
210 220 230
NPSEATLLGK PKYQDKVASS IYKGILRYFT EKGDPPE
Length:237
Mass (Da):27,006
Last modified:October 1, 1996 - v1
Checksum:iE5B92E58552B59D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74737 Genomic DNA. Translation: CAA52758.1.
D64126 Genomic DNA. Translation: BAA10993.1.
AL009126 Genomic DNA. Translation: CAB11929.1.
PIRiG69610.
RefSeqiNP_388034.1. NC_000964.3.
WP_004399672.1. NZ_JNCM01000029.1.

Genome annotation databases

EnsemblBacteriaiCAB11929; CAB11929; BSU01530.
GeneIDi938917.
KEGGibsu:BSU01530.
PATRICi18971817. VBIBacSub10457_0157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74737 Genomic DNA. Translation: CAA52758.1.
D64126 Genomic DNA. Translation: BAA10993.1.
AL009126 Genomic DNA. Translation: CAB11929.1.
PIRiG69610.
RefSeqiNP_388034.1. NC_000964.3.
WP_004399672.1. NZ_JNCM01000029.1.

3D structure databases

ProteinModelPortaliP50864.
SMRiP50864. Positions 41-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU01530.

Proteomic databases

PaxDbiP50864.

Protocols and materials databases

DNASUi938917.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11929; CAB11929; BSU01530.
GeneIDi938917.
KEGGibsu:BSU01530.
PATRICi18971817. VBIBacSub10457_0157.

Organism-specific databases

GenoListiBSU01530. [Micado]

Phylogenomic databases

eggNOGiCOG0860.
HOGENOMiHOG000163501.
InParanoidiP50864.
KOiK01448.
OMAiKCDAFIS.
OrthoDBiEOG6CP3X3.
PhylomeDBiP50864.

Enzyme and pathway databases

BioCyciBSUB:BSU01530-MONOMER.

Family and domain databases

Gene3Di3.40.630.40. 1 hit.
InterProiIPR002508. CW_Hdrlase/autolysin_cat.
IPR014234. Spore_CwlD.
[Graphical view]
PfamiPF01520. Amidase_3. 1 hit.
[Graphical view]
SMARTiSM00646. Ami_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02883. spore_cwlD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis."
    Sekiguchi J., Akeo K., Yamamoto H., Khasanov F.K., Alonso J.C., Kuroda A.
    J. Bacteriol. 177:5582-5589(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome."
    Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H.
    Microbiology 142:3039-3046(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan."
    Gilmore M.E., Bandyopadhyay D., Dean A.M., Linnstaedt S.D., Popham D.L.
    J. Bacteriol. 186:80-89(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCWLD_BACSU
AccessioniPrimary (citable) accession number: P50864
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 29, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

CwlD and PdaA are necessary and sufficient for muramic delta-lactam production in B.subtilis spore peptidoglycan.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.