Reviewed, UniProtKB/Swiss-Prot P50864 (CWLD_BACSU)

Names and origin

Protein names

Recommended name:
    Germination-specific N-acetylmuramoyl-L-alanine amidase
    EC=3.5.1.28
Alternative name(s):
    Cell wall hydrolase
    Autolysin

Gene names

Name:	cwlD
Ordered Locus Names:	BSU01530

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 237	210	Germination-specific N-acetylmuramoyl-L-alanine amidase		PRO_0000164419

Sequences

	Sequence		Length	Mass (Da)
	P50864-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E5B92E58552B59D6	FASTA	237	27,006
	10 20 30 40 50 60 MRKKLKWLSF LLGFIILLFL FKYQFSNNDS WKPWSLPLSG KIIYLDPGHG GPDGGAVGGK 70 80 90 100 110 120 LLEKDVTLEV AFRVRDYLQE QGALVIMTRE SDTDLAPEGT KGYSRRKAED LRQRVKLINH 130 140 150 160 170 180 SEAELYISIH LNAIPSQKWS GAQSFYYGKY AENEKVAKYI QDELRRNLEN TTRKAKRIHG 190 200 210 220 230 IYLMQNVTKP GALIEVGFLS NPSEATLLGK PKYQDKVASS IYKGILRYFT EKGDPPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis." Sekiguchi J., Akeo K., Yamamoto H., Khasanov F.K., Alonso J.C., Kuroda A. J. Bacteriol. 177:5582-5589(1995) [PubMed: 7559346] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome." Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H. Microbiology 142:3039-3046(1996) [PubMed: 8969501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74737 Genomic DNA. Translation: CAA52758.1. D64126 Genomic DNA. Translation: BAA10993.1. AL009126 Genomic DNA. Translation: CAB11929.1.
PIR	G69610.
RefSeq	NP_388034.1.
3D structure databases
SMR	P50864. Positions 41-232.
ModBase	Search...
Genome annotation databases
GeneID	938917.
GenomeReviews	Gene locus BSU01530 in contig AL009126_GR.
KEGG	bsu:BSU01530.
NMPDR	fig|224308.1.peg.153.
Organism-specific databases
SubtiList	BG11514. cwlD. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG656726.
OMA	SVIVECG.
Enzyme and pathway databases
BRENDA	3.5.1.28. 150.
Family and domain databases
InterPro	IPR002508. CW_Hdrlase/autolysin_cat. IPR014234. Spore_cwlD. [Graphical view]
Gene3D	G3DSA:3.40.630.40. Cell_wall_OHase/autolysin_cat. 1 hit.
Pfam	PF01520. Amidase_3. 1 hit. [Graphical view]
SMART	SM00646. Ami_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR02883. spore_cwlD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CWLD_BACSU

Accession

Primary (citable) accession number: P50864

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)