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P50864

- CWLD_BACSU

UniProt

P50864 - CWLD_BACSU

Protein

Germination-specific N-acetylmuramoyl-L-alanine amidase

Gene

cwlD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Cleaves the peptide side chain from the N-acetylmuramic acid residues in peptidoglycan. This is a step in the formation of muramic delta-lactam residues in spore cortex.1 Publication

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.1 Publication

    GO - Molecular functioni

    1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciBSUB:BSU01530-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Germination-specific N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
    Alternative name(s):
    Autolysin
    Cell wall hydrolase
    Gene namesi
    Name:cwlD
    Ordered Locus Names:BSU01530
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU01530. [Micado]

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 237210Germination-specific N-acetylmuramoyl-L-alanine amidasePRO_0000164419Add
    BLAST

    Proteomic databases

    PaxDbiP50864.

    Expressioni

    Developmental stagei

    Expression of cwlD takes place in both the mother cell and forespore compartments of sporulating cells.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU01530.

    Structurei

    3D structure databases

    ProteinModelPortaliP50864.
    SMRiP50864. Positions 41-232.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0860.
    HOGENOMiHOG000163501.
    KOiK01448.
    OMAiELYISIH.
    OrthoDBiEOG6CP3X3.
    PhylomeDBiP50864.

    Family and domain databases

    Gene3Di3.40.630.40. 1 hit.
    InterProiIPR002508. CW_Hdrlase/autolysin_cat.
    IPR014234. Spore_CwlD.
    [Graphical view]
    PfamiPF01520. Amidase_3. 1 hit.
    [Graphical view]
    SMARTiSM00646. Ami_3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02883. spore_cwlD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50864-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKKLKWLSF LLGFIILLFL FKYQFSNNDS WKPWSLPLSG KIIYLDPGHG    50
    GPDGGAVGGK LLEKDVTLEV AFRVRDYLQE QGALVIMTRE SDTDLAPEGT 100
    KGYSRRKAED LRQRVKLINH SEAELYISIH LNAIPSQKWS GAQSFYYGKY 150
    AENEKVAKYI QDELRRNLEN TTRKAKRIHG IYLMQNVTKP GALIEVGFLS 200
    NPSEATLLGK PKYQDKVASS IYKGILRYFT EKGDPPE 237
    Length:237
    Mass (Da):27,006
    Last modified:October 1, 1996 - v1
    Checksum:iE5B92E58552B59D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74737 Genomic DNA. Translation: CAA52758.1.
    D64126 Genomic DNA. Translation: BAA10993.1.
    AL009126 Genomic DNA. Translation: CAB11929.1.
    PIRiG69610.
    RefSeqiNP_388034.1. NC_000964.3.
    WP_004399672.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB11929; CAB11929; BSU01530.
    GeneIDi938917.
    KEGGibsu:BSU01530.
    PATRICi18971817. VBIBacSub10457_0157.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74737 Genomic DNA. Translation: CAA52758.1 .
    D64126 Genomic DNA. Translation: BAA10993.1 .
    AL009126 Genomic DNA. Translation: CAB11929.1 .
    PIRi G69610.
    RefSeqi NP_388034.1. NC_000964.3.
    WP_004399672.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P50864.
    SMRi P50864. Positions 41-232.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU01530.

    Proteomic databases

    PaxDbi P50864.

    Protocols and materials databases

    DNASUi 938917.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB11929 ; CAB11929 ; BSU01530 .
    GeneIDi 938917.
    KEGGi bsu:BSU01530.
    PATRICi 18971817. VBIBacSub10457_0157.

    Organism-specific databases

    GenoListi BSU01530. [Micado ]

    Phylogenomic databases

    eggNOGi COG0860.
    HOGENOMi HOG000163501.
    KOi K01448.
    OMAi ELYISIH.
    OrthoDBi EOG6CP3X3.
    PhylomeDBi P50864.

    Enzyme and pathway databases

    BioCyci BSUB:BSU01530-MONOMER.

    Family and domain databases

    Gene3Di 3.40.630.40. 1 hit.
    InterProi IPR002508. CW_Hdrlase/autolysin_cat.
    IPR014234. Spore_CwlD.
    [Graphical view ]
    Pfami PF01520. Amidase_3. 1 hit.
    [Graphical view ]
    SMARTi SM00646. Ami_3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02883. spore_cwlD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis."
      Sekiguchi J., Akeo K., Yamamoto H., Khasanov F.K., Alonso J.C., Kuroda A.
      J. Bacteriol. 177:5582-5589(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome."
      Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H.
      Microbiology 142:3039-3046(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan."
      Gilmore M.E., Bandyopadhyay D., Dean A.M., Linnstaedt S.D., Popham D.L.
      J. Bacteriol. 186:80-89(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiCWLD_BACSU
    AccessioniPrimary (citable) accession number: P50864
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    CwlD and PdaA are necessary and sufficient for muramic delta-lactam production in B.subtilis spore peptidoglycan.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3