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P50864

- CWLD_BACSU

UniProt

P50864 - CWLD_BACSU

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Protein

Germination-specific N-acetylmuramoyl-L-alanine amidase

Gene
cwlD, BSU01530
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves the peptide side chain from the N-acetylmuramic acid residues in peptidoglycan. This is a step in the formation of muramic delta-lactam residues in spore cortex.1 Publication

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.1 Publication

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

GO - Biological processi

  1. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciBSUB:BSU01530-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Germination-specific N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Alternative name(s):
Autolysin
Cell wall hydrolase
Gene namesi
Name:cwlD
Ordered Locus Names:BSU01530
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU01530. [Micado]

Subcellular locationi

Secreted Reviewed prediction

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Chaini28 – 237210Germination-specific N-acetylmuramoyl-L-alanine amidasePRO_0000164419Add
BLAST

Proteomic databases

PaxDbiP50864.

Expressioni

Developmental stagei

Expression of cwlD takes place in both the mother cell and forespore compartments of sporulating cells.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU01530.

Structurei

3D structure databases

ProteinModelPortaliP50864.
SMRiP50864. Positions 41-232.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0860.
HOGENOMiHOG000163501.
KOiK01448.
OMAiELYISIH.
OrthoDBiEOG6CP3X3.
PhylomeDBiP50864.

Family and domain databases

Gene3Di3.40.630.40. 1 hit.
InterProiIPR002508. CW_Hdrlase/autolysin_cat.
IPR014234. Spore_CwlD.
[Graphical view]
PfamiPF01520. Amidase_3. 1 hit.
[Graphical view]
SMARTiSM00646. Ami_3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02883. spore_cwlD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50864-1 [UniParc]FASTAAdd to Basket

« Hide

MRKKLKWLSF LLGFIILLFL FKYQFSNNDS WKPWSLPLSG KIIYLDPGHG    50
GPDGGAVGGK LLEKDVTLEV AFRVRDYLQE QGALVIMTRE SDTDLAPEGT 100
KGYSRRKAED LRQRVKLINH SEAELYISIH LNAIPSQKWS GAQSFYYGKY 150
AENEKVAKYI QDELRRNLEN TTRKAKRIHG IYLMQNVTKP GALIEVGFLS 200
NPSEATLLGK PKYQDKVASS IYKGILRYFT EKGDPPE 237
Length:237
Mass (Da):27,006
Last modified:October 1, 1996 - v1
Checksum:iE5B92E58552B59D6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74737 Genomic DNA. Translation: CAA52758.1.
D64126 Genomic DNA. Translation: BAA10993.1.
AL009126 Genomic DNA. Translation: CAB11929.1.
PIRiG69610.
RefSeqiNP_388034.1. NC_000964.3.
WP_004399672.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB11929; CAB11929; BSU01530.
GeneIDi938917.
KEGGibsu:BSU01530.
PATRICi18971817. VBIBacSub10457_0157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74737 Genomic DNA. Translation: CAA52758.1 .
D64126 Genomic DNA. Translation: BAA10993.1 .
AL009126 Genomic DNA. Translation: CAB11929.1 .
PIRi G69610.
RefSeqi NP_388034.1. NC_000964.3.
WP_004399672.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali P50864.
SMRi P50864. Positions 41-232.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU01530.

Proteomic databases

PaxDbi P50864.

Protocols and materials databases

DNASUi 938917.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB11929 ; CAB11929 ; BSU01530 .
GeneIDi 938917.
KEGGi bsu:BSU01530.
PATRICi 18971817. VBIBacSub10457_0157.

Organism-specific databases

GenoListi BSU01530. [Micado ]

Phylogenomic databases

eggNOGi COG0860.
HOGENOMi HOG000163501.
KOi K01448.
OMAi ELYISIH.
OrthoDBi EOG6CP3X3.
PhylomeDBi P50864.

Enzyme and pathway databases

BioCyci BSUB:BSU01530-MONOMER.

Family and domain databases

Gene3Di 3.40.630.40. 1 hit.
InterProi IPR002508. CW_Hdrlase/autolysin_cat.
IPR014234. Spore_CwlD.
[Graphical view ]
Pfami PF01520. Amidase_3. 1 hit.
[Graphical view ]
SMARTi SM00646. Ami_3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02883. spore_cwlD. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis."
    Sekiguchi J., Akeo K., Yamamoto H., Khasanov F.K., Alonso J.C., Kuroda A.
    J. Bacteriol. 177:5582-5589(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome."
    Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H.
    Microbiology 142:3039-3046(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan."
    Gilmore M.E., Bandyopadhyay D., Dean A.M., Linnstaedt S.D., Popham D.L.
    J. Bacteriol. 186:80-89(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCWLD_BACSU
AccessioniPrimary (citable) accession number: P50864
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

CwlD and PdaA are necessary and sufficient for muramic delta-lactam production in B.subtilis spore peptidoglycan (1 Publication).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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