Reviewed, UniProtKB/Swiss-Prot P50859 (CP51_CANGA)

Names and origin

Protein names

Recommended name:
    Lanosterol 14-alpha demethylase
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    CYPLI
    P450-LIA1
    Sterol 14-alpha demethylase
    P450-14DM

Gene names

Name:	ERG11
Synonyms:	CYP51
Ordered Locus Names:	CAGL0E04334g

Organism

Candida glabrata (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

5478 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	Lanosterol 14-alpha demethylase		PRO_0000052004
Sites
	Metal binding	472	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	64	1	I → M in AAB32679. Ref.3
	Sequence conflict	473	1	I → T in AAB32679. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P50859-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A0506C17507E6EF7	FASTA	533	61,305
	10 20 30 40 50 60 MSTENTSLVV ELLEYVKLGL SYFQALPLAQ RVSIMVALPF VYTITWQLLY SLRKDRPPLV 70 80 90 100 110 120 FYWIPWVGSA IPYGTKPYEF FEDCQKKYGD IFSFMLLGRI MTVYLGPKGH EFIFNAKLAD 130 140 150 160 170 180 VSAEAAYSHL TTPVFGKGVI YDCPNHRLME QKKFVKGALT KEAFVRYVPL IAEEIYKYFR 190 200 210 220 230 240 NSKNFKINEN NSGIVDVMVS QPEMTIFTAS RSLLGKEMRD KLDTDFAYLY SDLDKGFTPI 250 260 270 280 290 300 NFVFPNLPLE HYRKRDHAQQ AISGTYMSLI KERREKNDIQ NRDLIDELMK NSTYKDGTKM 310 320 330 340 350 360 TDQEIANLLI GVLMGGQHTS AATSAWCLLH LAERPDVQEE LYQEQMRVLN NDTKELTYDD 370 380 390 400 410 420 LQNMPLLNQM IKETLRLHHP LHSLFRKVMR DVAIPNTSYV VPRDYHVLVS PGYTHLQEEF 430 440 450 460 470 480 FPKPNEFNIH RWDGDAASSS AAGGDEVDYG FGAISKGVSS PYLPFGGGRH RCIGELFAYC 490 500 510 520 530 QLGVLMSIFI RTMKWRYPTE GETVPPSDFT SMVTLPTAPA KIYWEKRHPE QKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility." Geber A., Hitchcock C.A., Swartz J.E., Pullen F.S., Marsden K.E., Kwon-Chung K.J., Bennett J.E. Antimicrob. Agents Chemother. 39:2708-2717(1995) [PubMed: 8593007] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2001-L5.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65.
[3]	"Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment." Burgener-Kairuz P., Zuber J.P., Jaunin P., Buchman T.G., Bille J., Rossier M. J. Clin. Microbiol. 32:1902-1907(1994) [PubMed: 7989540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-473. Strain: ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L40389 Genomic DNA. Translation: AAB02329.1. CR380951 Genomic DNA. Translation: CAG58795.1. S75389 Genomic DNA. Translation: AAB32679.1.
RefSeq	XP_445876.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P50859.
Genome annotation databases
GeneID	2887532.
GenomeReviews	Gene locus CAGL0E04334g in contig CR380951_GR.
KEGG	cgr:CAGL0E04334g.
Phylogenomic databases
HOGENOM	P50859.
OMA	NFVFPNL.
Enzyme and pathway databases
BRENDA	1.14.13.70. 189220.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00639. Butoconazole. DB01167. Itraconazole. DB01110. Miconazole.

Entry information

Entry name

CP51_CANGA

Accession

Primary (citable) accession number: P50859
Secondary accession number(s): Q02312

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)