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Reviewed, UniProtKB/Swiss-Prot P50859 (CP51_CANGA)

Last modified November 3, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Lanosterol 14-alpha demethylase
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    CYPLI
    P450-LIA1
    Sterol 14-alpha demethylase
    P450-14DM
Gene names
Name: ERG11
Synonyms: CYP51
Ordered Locus Names: CAGL0E04334g
OrganismCandida glabrata (Yeast) (Torulopsis glabrata) [Complete proteome]
Taxonomic identifier5478 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesmitosporic Nakaseomyces

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.

Catalytic activity

Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Pathway

Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.

Subcellular location

Membrane Potential. Membrane; Single-pass membrane protein.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Lanosterol 14-alpha demethylase
PRO_0000052004

Sites

Metal binding4721Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict641I → M in AAB32679. Ref.3
Sequence conflict4731I → T in AAB32679. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P50859-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: A0506C17507E6EF7

FASTA53361,305
        10         20         30         40         50         60 
MSTENTSLVV ELLEYVKLGL SYFQALPLAQ RVSIMVALPF VYTITWQLLY SLRKDRPPLV 

        70         80         90        100        110        120 
FYWIPWVGSA IPYGTKPYEF FEDCQKKYGD IFSFMLLGRI MTVYLGPKGH EFIFNAKLAD 

       130        140        150        160        170        180 
VSAEAAYSHL TTPVFGKGVI YDCPNHRLME QKKFVKGALT KEAFVRYVPL IAEEIYKYFR 

       190        200        210        220        230        240 
NSKNFKINEN NSGIVDVMVS QPEMTIFTAS RSLLGKEMRD KLDTDFAYLY SDLDKGFTPI 

       250        260        270        280        290        300 
NFVFPNLPLE HYRKRDHAQQ AISGTYMSLI KERREKNDIQ NRDLIDELMK NSTYKDGTKM 

       310        320        330        340        350        360 
TDQEIANLLI GVLMGGQHTS AATSAWCLLH LAERPDVQEE LYQEQMRVLN NDTKELTYDD 

       370        380        390        400        410        420 
LQNMPLLNQM IKETLRLHHP LHSLFRKVMR DVAIPNTSYV VPRDYHVLVS PGYTHLQEEF 

       430        440        450        460        470        480 
FPKPNEFNIH RWDGDAASSS AAGGDEVDYG FGAISKGVSS PYLPFGGGRH RCIGELFAYC 

       490        500        510        520        530 
QLGVLMSIFI RTMKWRYPTE GETVPPSDFT SMVTLPTAPA KIYWEKRHPE QKY 

« Hide

References

« Hide 'large scale' references
[1]"Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility."
Geber A., Hitchcock C.A., Swartz J.E., Pullen F.S., Marsden K.E., Kwon-Chung K.J., Bennett J.E.
Antimicrob. Agents Chemother. 39:2708-2717(1995) [PubMed: 8593007] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 2001-L5.
[2]"Genome evolution in yeasts."
Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. expand/collapse author list , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
Nature 430:35-44(2004) [PubMed: 15229592] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65.
[3]"Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment."
Burgener-Kairuz P., Zuber J.P., Jaunin P., Buchman T.G., Bille J., Rossier M.
J. Clin. Microbiol. 32:1902-1907(1994) [PubMed: 7989540] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-473.
Strain: ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65.

Cross-references

Sequence databases

L40389 Genomic DNA. Translation: AAB02329.1.
CR380951 Genomic DNA. Translation: CAG58795.1.
S75389 Genomic DNA. Translation: AAB32679.1.
RefSeqXP_445876.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP50859.

Genome annotation databases

GeneID2887532.
GenomeReviewsGene locus CAGL0E04334g in contig CR380951_GR.
KEGGcgr:CAGL0E04334g.

Phylogenomic databases

HOGENOMP50859.
OMANFVFPNL.

Enzyme and pathway databases

BRENDA1.14.13.70. 189220.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR017973. Cyt_P450_C.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00465. EP450IV.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00639. Butoconazole.
DB01167. Itraconazole.
DB01110. Miconazole.

Entry information

Entry nameCP51_CANGA
AccessionPrimary (citable) accession number: P50859
Secondary accession number(s): Q02312
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents