P50852 (PTMCB_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PTS system mannitol-specific EIICB component

Alternative name(s):
EIICB-Mtl
Short name=EII-Mtl

Including the following 2 domains:

1. Mannitol permease IIC component
   Alternative name(s):
   PTS system mannitol-specific EIIC component
2. Mannitol-specific phosphotransferase enzyme IIB component
   EC=2.7.1.69
   Alternative name(s):
   PTS system mannitol-specific EIIB component

Gene names

Name:

mtlA

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	PTS system mannitol-specific EIICB component		PRO_0000186609
Regions
	Transmembrane	30 – 50	21	Helical; Potential
	Transmembrane	54 – 74	21	Helical; Potential
	Transmembrane	92 – 112	21	Helical; Potential
	Transmembrane	143 – 163	21	Helical; Potential
	Transmembrane	219 – 239	21	Helical; Potential
	Transmembrane	251 – 271	21	Helical; Potential
	Transmembrane	273 – 293	21	Helical; Potential
	Transmembrane	319 – 339	21	Helical; Potential
	Domain	18 – 342	325	PTS EIIC type-2
	Domain	383 – 471	89	PTS EIIB type-2
Sites
	Active site	389	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50852 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3BF2F9F3C8FD98CB	FASTA	471	50,154
	10 20 30 40 50 60 MTHTSENQAG FRVKIQRFGS YLSGMIMPNI GAFIAWGIIT ALFIPTGWLP NETFAKLVGP 70 80 90 100 110 120 MITYLLPLLI GYTGGKMIYD VRGGVVGATA TMGVIVGSDI PMFLGAMIMG PLGGYLIKKF 130 140 150 160 170 180 DQQIQGKVKQ GFEMLVNNFS AGIIGGLLTL AAFKGVGPVV SAISKTLAAG VEKIVDLHLL 190 200 210 220 230 240 PLANIFIEPG KVLFLNNAIN HGILSPLGIE QAAKTGKSIL FLLEPNPGPG LGILLAYWLF 250 260 270 280 290 300 GKGMAKQSAP GAIIIHFLGG IHEIYFPYVL MRPILILAAI AGGVSGVLTF TIFDAGLVAV 310 320 330 340 350 360 PSPGSIFALL AMTPKGNYLG VLAGVLVATA VSFFVASIFL KSAKNNEEDI TKATEKMQQL 370 380 390 400 410 420 KGKKSDVVAV LKNEEKVIPA KVKKIVFACD AGMGSSAMGA SILRNKMQKA GLNIEVTNTA 430 440 450 460 470 INQLPEDADI VITHQNLTDR AKEKLPKAFH ISVENFLNSP KYDELIEMLK K

References

[1]

"Cloning, expression, and isolation of the mannitol transport protein from the thermophilic bacterium Bacillus stearothermophilus." Henstra S.A., Tolner B., ten Hoeve Duurkens R.H., Konings W.N., Robillard G.T. J. Bacteriol. 178:5586-5591(1996) [PubMed: 8824601] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18943 Genomic DNA. Translation: AAC44463.1.
3D structure databases
ProteinModelPortal	P50852.
SMR	P50852. Positions 380-469.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR004718. PTS_IIC_mtl. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00851. MtlA. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTMCB_GEOSE

Accession

Primary (citable) accession number: P50852

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 31, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program