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Protein

KHG/KDPG aldolase

Gene

kdgA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.
2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate.

Pathwayi: 2-dehydro-3-deoxy-D-gluconate degradation

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 2-dehydro-3-deoxygluconokinase (kdgK)
  2. KHG/KDPG aldolase (kdgA)
This subpathway is part of the pathway 2-dehydro-3-deoxy-D-gluconate degradation, which is itself part of Carbohydrate acid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate, the pathway 2-dehydro-3-deoxy-D-gluconate degradation and in Carbohydrate acid metabolism.

Pathwayi: glyoxylate and dicarboxylate metabolism

This protein is involved in the pathway glyoxylate and dicarboxylate metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway glyoxylate and dicarboxylate metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei43By similarity1
Active sitei47By similarity1
Active sitei130Schiff-base intermediate with KHG or pyruvateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciBSUB:BSU22100-MONOMER.
UniPathwayiUPA00227.
UPA00856; UER00829.

Names & Taxonomyi

Protein namesi
Recommended name:
KHG/KDPG aldolase
Including the following 2 domains:
4-hydroxy-2-oxoglutarate aldolase (EC:4.1.3.16)
Alternative name(s):
2-keto-4-hydroxyglutarate aldolase
Short name:
KHG-aldolase
2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14)
Alternative name(s):
2-keto-3-deoxy-6-phosphogluconate aldolase
Short name:
KDPG-aldolase
Phospho-2-dehydro-3-deoxygluconate aldolase
Phospho-2-keto-3-deoxygluconate aldolase
Gene namesi
Name:kdgA
Ordered Locus Names:BSU22100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002010361 – 196KHG/KDPG aldolaseAdd BLAST196

Proteomic databases

PaxDbiP50846.

Expressioni

Inductioni

Induced by galacturonate and negatively regulated by the KdgR repressor. Is subject to catabolite repression by glucose involving the ccpA gene.1 Publication

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012166.

Structurei

3D structure databases

ProteinModelPortaliP50846.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KHG/KDPG aldolase family.Curated

Phylogenomic databases

eggNOGiENOG4108UHU. Bacteria.
COG0800. LUCA.
HOGENOMiHOG000233113.
InParanoidiP50846.
KOiK01625.
OMAiSFRNRED.
PhylomeDBiP50846.

Family and domain databases

CDDicd00452. KDPG_aldolase. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
IPR031338. KDPG/KHG_AS_2.
[Graphical view]
PfamiPF01081. Aldolase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01182. eda. 1 hit.
PROSITEiPS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESKVVENRL KEAKLIAVIR SKDKQEACQQ IESLLDKGIR AVEVTYTTPG
60 70 80 90 100
ASDIIESFRN REDILIGAGT VISAQQAGEA AKAGAQFIVS PGFSADLAEH
110 120 130 140 150
LSFVKTHYIP GVLTPSEIME ALTFGFTTLK LFPSGVFGIP FMKNLAGPFP
160 170 180 190
QVTFIPTGGI HPSEVPDWLR AGAGAVGVGS QLGSCSKEDL QAVFQV
Length:196
Mass (Da):20,865
Last modified:October 1, 1996 - v1
Checksum:iEB0B597FD1102CC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47838 Genomic DNA. Translation: AAB38480.1.
AL009126 Genomic DNA. Translation: CAB14127.1.
PIRiH69647.
RefSeqiNP_390092.1. NC_000964.3.
WP_003230732.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14127; CAB14127; BSU22100.
GeneIDi939066.
KEGGibsu:BSU22100.
PATRICi18976227. VBIBacSub10457_2304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47838 Genomic DNA. Translation: AAB38480.1.
AL009126 Genomic DNA. Translation: CAB14127.1.
PIRiH69647.
RefSeqiNP_390092.1. NC_000964.3.
WP_003230732.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP50846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012166.

Proteomic databases

PaxDbiP50846.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14127; CAB14127; BSU22100.
GeneIDi939066.
KEGGibsu:BSU22100.
PATRICi18976227. VBIBacSub10457_2304.

Phylogenomic databases

eggNOGiENOG4108UHU. Bacteria.
COG0800. LUCA.
HOGENOMiHOG000233113.
InParanoidiP50846.
KOiK01625.
OMAiSFRNRED.
PhylomeDBiP50846.

Enzyme and pathway databases

UniPathwayiUPA00227.
UPA00856; UER00829.
BioCyciBSUB:BSU22100-MONOMER.

Family and domain databases

CDDicd00452. KDPG_aldolase. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
IPR031338. KDPG/KHG_AS_2.
[Graphical view]
PfamiPF01081. Aldolase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01182. eda. 1 hit.
PROSITEiPS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALKH_BACSU
AccessioniPrimary (citable) accession number: P50846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 5, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.