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P50846

- ALKH_BACSU

UniProt

P50846 - ALKH_BACSU

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Protein

KHG/KDPG aldolase

Gene

kdgA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.
2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei43 – 431By similarity
Active sitei47 – 471By similarity
Active sitei130 – 1301Schiff-base intermediate with KHG or pyruvateBy similarity

GO - Molecular functioni

  1. 2-dehydro-3-deoxy-phosphogluconate aldolase activity Source: UniProtKB-EC
  2. 4-hydroxy-2-oxoglutarate aldolase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciBSUB:BSU22100-MONOMER.
UniPathwayiUPA00227.
UPA00856; UER00829.

Names & Taxonomyi

Protein namesi
Recommended name:
KHG/KDPG aldolase
Including the following 2 domains:
4-hydroxy-2-oxoglutarate aldolase (EC:4.1.3.16)
Alternative name(s):
2-keto-4-hydroxyglutarate aldolase
Short name:
KHG-aldolase
2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14)
Alternative name(s):
2-keto-3-deoxy-6-phosphogluconate aldolase
Short name:
KDPG-aldolase
Phospho-2-dehydro-3-deoxygluconate aldolase
Phospho-2-keto-3-deoxygluconate aldolase
Gene namesi
Name:kdgA
Ordered Locus Names:BSU22100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU22100. [Micado]

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196KHG/KDPG aldolasePRO_0000201036Add
BLAST

Proteomic databases

PaxDbiP50846.

Expressioni

Inductioni

Induced by galacturonate and negatively regulated by the KdgR repressor. Is subject to catabolite repression by glucose involving the ccpA gene.1 Publication

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi224308.BSU22100.

Structurei

3D structure databases

ProteinModelPortaliP50846.
SMRiP50846. Positions 2-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KHG/KDPG aldolase family.Curated

Phylogenomic databases

eggNOGiCOG0800.
HOGENOMiHOG000233113.
InParanoidiP50846.
KOiK01625.
OMAiQALACIE.
OrthoDBiEOG6K9QNZ.
PhylomeDBiP50846.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
[Graphical view]
PfamiPF01081. Aldolase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01182. eda. 1 hit.
PROSITEiPS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50846-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESKVVENRL KEAKLIAVIR SKDKQEACQQ IESLLDKGIR AVEVTYTTPG
60 70 80 90 100
ASDIIESFRN REDILIGAGT VISAQQAGEA AKAGAQFIVS PGFSADLAEH
110 120 130 140 150
LSFVKTHYIP GVLTPSEIME ALTFGFTTLK LFPSGVFGIP FMKNLAGPFP
160 170 180 190
QVTFIPTGGI HPSEVPDWLR AGAGAVGVGS QLGSCSKEDL QAVFQV
Length:196
Mass (Da):20,865
Last modified:October 1, 1996 - v1
Checksum:iEB0B597FD1102CC4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L47838 Genomic DNA. Translation: AAB38480.1.
AL009126 Genomic DNA. Translation: CAB14127.1.
PIRiH69647.
RefSeqiNP_390092.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14127; CAB14127; BSU22100.
GeneIDi939066.
KEGGibsu:BSU22100.
PATRICi18976227. VBIBacSub10457_2304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L47838 Genomic DNA. Translation: AAB38480.1 .
AL009126 Genomic DNA. Translation: CAB14127.1 .
PIRi H69647.
RefSeqi NP_390092.1. NC_000964.3.

3D structure databases

ProteinModelPortali P50846.
SMRi P50846. Positions 2-193.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU22100.

Proteomic databases

PaxDbi P50846.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14127 ; CAB14127 ; BSU22100 .
GeneIDi 939066.
KEGGi bsu:BSU22100.
PATRICi 18976227. VBIBacSub10457_2304.

Organism-specific databases

GenoListi BSU22100. [Micado ]

Phylogenomic databases

eggNOGi COG0800.
HOGENOMi HOG000233113.
InParanoidi P50846.
KOi K01625.
OMAi QALACIE.
OrthoDBi EOG6K9QNZ.
PhylomeDBi P50846.

Enzyme and pathway databases

UniPathwayi UPA00227 .
UPA00856 ; UER00829 .
BioCyci BSUB:BSU22100-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
[Graphical view ]
Pfami PF01081. Aldolase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01182. eda. 1 hit.
PROSITEi PS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome."
    Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P.
    Microbiology 142:2005-2016(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "The kdgRKAT operon of Bacillus subtilis: detection of the transcript and regulation by the kdgR and ccpA genes."
    Pujic P., Dervyn R., Sorokin A., Ehrlich S.D.
    Microbiology 144:3111-3118(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: 168.

Entry informationi

Entry nameiALKH_BACSU
AccessioniPrimary (citable) accession number: P50846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3