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P50846

- ALKH_BACSU

UniProt

P50846 - ALKH_BACSU

Protein

KHG/KDPG aldolase

Gene

kdgA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.
    2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei43 – 431By similarity
    Active sitei47 – 471By similarity
    Active sitei130 – 1301Schiff-base intermediate with KHG or pyruvateBy similarity

    GO - Molecular functioni

    1. 2-dehydro-3-deoxy-phosphogluconate aldolase activity Source: UniProtKB-EC
    2. 4-hydroxy-2-oxoglutarate aldolase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciBSUB:BSU22100-MONOMER.
    UniPathwayiUPA00227.
    UPA00856; UER00829.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    KHG/KDPG aldolase
    Including the following 2 domains:
    4-hydroxy-2-oxoglutarate aldolase (EC:4.1.3.16)
    Alternative name(s):
    2-keto-4-hydroxyglutarate aldolase
    Short name:
    KHG-aldolase
    2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14)
    Alternative name(s):
    2-keto-3-deoxy-6-phosphogluconate aldolase
    Short name:
    KDPG-aldolase
    Phospho-2-dehydro-3-deoxygluconate aldolase
    Phospho-2-keto-3-deoxygluconate aldolase
    Gene namesi
    Name:kdgA
    Ordered Locus Names:BSU22100
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU22100. [Micado]

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 196196KHG/KDPG aldolasePRO_0000201036Add
    BLAST

    Proteomic databases

    PaxDbiP50846.

    Expressioni

    Inductioni

    Induced by galacturonate and negatively regulated by the KdgR repressor. Is subject to catabolite repression by glucose involving the ccpA gene.1 Publication

    Interactioni

    Subunit structurei

    Homotrimer.By similarity

    Protein-protein interaction databases

    STRINGi224308.BSU22100.

    Structurei

    3D structure databases

    ProteinModelPortaliP50846.
    SMRiP50846. Positions 2-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the KHG/KDPG aldolase family.Curated

    Phylogenomic databases

    eggNOGiCOG0800.
    HOGENOMiHOG000233113.
    KOiK01625.
    OMAiQALACIE.
    OrthoDBiEOG6K9QNZ.
    PhylomeDBiP50846.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR000887. Aldlse_KDPG_KHG.
    IPR013785. Aldolase_TIM.
    [Graphical view]
    PfamiPF01081. Aldolase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01182. eda. 1 hit.
    PROSITEiPS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50846-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESKVVENRL KEAKLIAVIR SKDKQEACQQ IESLLDKGIR AVEVTYTTPG    50
    ASDIIESFRN REDILIGAGT VISAQQAGEA AKAGAQFIVS PGFSADLAEH 100
    LSFVKTHYIP GVLTPSEIME ALTFGFTTLK LFPSGVFGIP FMKNLAGPFP 150
    QVTFIPTGGI HPSEVPDWLR AGAGAVGVGS QLGSCSKEDL QAVFQV 196
    Length:196
    Mass (Da):20,865
    Last modified:October 1, 1996 - v1
    Checksum:iEB0B597FD1102CC4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47838 Genomic DNA. Translation: AAB38480.1.
    AL009126 Genomic DNA. Translation: CAB14127.1.
    PIRiH69647.
    RefSeqiNP_390092.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB14127; CAB14127; BSU22100.
    GeneIDi939066.
    KEGGibsu:BSU22100.
    PATRICi18976227. VBIBacSub10457_2304.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47838 Genomic DNA. Translation: AAB38480.1 .
    AL009126 Genomic DNA. Translation: CAB14127.1 .
    PIRi H69647.
    RefSeqi NP_390092.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P50846.
    SMRi P50846. Positions 2-193.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU22100.

    Proteomic databases

    PaxDbi P50846.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14127 ; CAB14127 ; BSU22100 .
    GeneIDi 939066.
    KEGGi bsu:BSU22100.
    PATRICi 18976227. VBIBacSub10457_2304.

    Organism-specific databases

    GenoListi BSU22100. [Micado ]

    Phylogenomic databases

    eggNOGi COG0800.
    HOGENOMi HOG000233113.
    KOi K01625.
    OMAi QALACIE.
    OrthoDBi EOG6K9QNZ.
    PhylomeDBi P50846.

    Enzyme and pathway databases

    UniPathwayi UPA00227 .
    UPA00856 ; UER00829 .
    BioCyci BSUB:BSU22100-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR000887. Aldlse_KDPG_KHG.
    IPR013785. Aldolase_TIM.
    [Graphical view ]
    Pfami PF01081. Aldolase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01182. eda. 1 hit.
    PROSITEi PS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome."
      Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P.
      Microbiology 142:2005-2016(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "The kdgRKAT operon of Bacillus subtilis: detection of the transcript and regulation by the kdgR and ccpA genes."
      Pujic P., Dervyn R., Sorokin A., Ehrlich S.D.
      Microbiology 144:3111-3118(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: 168.

    Entry informationi

    Entry nameiALKH_BACSU
    AccessioniPrimary (citable) accession number: P50846
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3