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P50828 (HEMO_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hemopexin
Alternative name(s):
Hyaluronidase
EC=3.2.1.35
Gene names
Name:HPX
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length459 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

Catalytic activity

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Subcellular location

Secreted.

Tissue specificity

Expressed by the liver and secreted in plasma.

Miscellaneous

The isolated N-terminal domain binds one heme. The full-length protein also binds one heme, but at a different site. The physiological significance of this is not clear By similarity.

Sequence similarities

Belongs to the hemopexin family.

Contains 8 hemopexin repeats.

Caution

Lacks the conserved His heme iron ligand in position 81. There is a Gln in this position.

Ontologies

Keywords
   Biological processTransport
   Cellular componentSecreted
   DomainRepeat
Signal
   LigandHeme
Iron
Metal-binding
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhyalurononglucosaminidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.1
Chain29 – 459431Hemopexin
PRO_0000021408

Regions

Repeat55 – 9541Hemopexin 1
Repeat96 – 14045Hemopexin 2
Repeat141 – 18545Hemopexin 3
Repeat186 – 23247Hemopexin 4
Repeat252 – 29746Hemopexin 5
Repeat298 – 34548Hemopexin 6
Repeat350 – 38940Hemopexin 7
Repeat393 – 44351Hemopexin 8

Sites

Metal binding1511Iron (heme 1 axial ligand) By similarity
Metal binding2371Iron (heme 2 axial ligand) By similarity
Metal binding2861Iron (heme 2 axial ligand) By similarity

Amino acid modifications

Glycosylation401N-linked (GlcNAc...) Potential
Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation1881N-linked (GlcNAc...) Potential
Glycosylation2411N-linked (GlcNAc...) Potential
Disulfide bond52 ↔ 232 By similarity
Disulfide bond150 ↔ 155 By similarity
Disulfide bond189 ↔ 201 By similarity
Disulfide bond250 ↔ 453 By similarity
Disulfide bond359 ↔ 401 By similarity
Disulfide bond411 ↔ 428 By similarity

Sequences

Sequence LengthMass (Da)Tools
P50828 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: DB06BB44C29789CF

FASTA45951,306
        10         20         30         40         50         60 
MARALGTVEA PWLLGLCCSL AIAHPLSLTA GPKHGAEGRN ESKPDPDVTE RCSDGWGFDA 

        70         80         90        100        110        120 
STLDEHGAML FFKGPSVWAG QNWTRGLISE RWKNAPSSVD AAFRRGHDRV FLIQGDKVWV 

       130        140        150        160        170        180 
YPPEKEKENP RSLQEEFPGV PSPLDAAVEC HRGECQDEGV LFFQGTHTWF WDSTTKTTKE 

       190        200        210        220        230        240 
RLWPAVGNCS SAMRWISRYY CFRGNQFLRF DPVTGHVDPK YPRDVRDYFM SCPGRGHAHR 

       250        260        270        280        290        300 
NATHRGDDRC SPDLVLTALL SDNHGATYAF RGTHYWRLDT SRDGWHSWPI DHQWSHGPSA 

       310        320        330        340        350        360 
VDAAFSWDDK LYLIQGTQVY IFLTKAGYTL VDNYPKQLEK ELGSPHGISL DAVDATFVCP 

       370        380        390        400        410        420 
GTSRLHVMAG RKLWWLDLSL GAQGPWTELP WPHEKVDAAL CTEKSLGPNS CSASGLGLYI 

       430        440        450 
VHGPHVYCYK DVEKLVSAKA LPQPQSVNSL LGCHRSRGS 

« Hide

References

[1]"Molecular cloning of a mammalian hyaluronidase reveals identity with hemopexin, a serum heme-binding protein."
Zhu L., Hope T.J., Hall J., Davies A., Stern M., Mueller-Eberhard U., Stern R., Parslow T.G.
J. Biol. Chem. 269:32092-32097(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-48 AND 368-388.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U14751 mRNA. Translation: AAC48457.1.
PIRA55486.
RefSeqNP_999118.1. NM_213953.2.
UniGeneSsc.4250.

3D structure databases

ProteinModelPortalP50828.
SMRP50828. Positions 50-453.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000015556.

Proteomic databases

PaxDbP50828.
PRIDEP50828.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396998.
KEGGssc:396998.

Organism-specific databases

CTD3263.

Phylogenomic databases

eggNOGNOG251805.
HOGENOMHOG000112887.
HOVERGENHBG005956.

Family and domain databases

Gene3D2.110.10.10. 2 hits.
InterProIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR016358. Hemopexin_chordata.
IPR018486. Hemopexin_CS.
[Graphical view]
PfamPF00045. Hemopexin. 3 hits.
[Graphical view]
PIRSFPIRSF002551. Hemopexin_chordata. 1 hit.
SMARTSM00120. HX. 5 hits.
[Graphical view]
SUPFAMSSF50923. SSF50923. 2 hits.
PROSITEPS00024. HEMOPEXIN. 2 hits.
PS51642. HEMOPEXIN_2. 8 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEMO_PIG
AccessionPrimary (citable) accession number: P50828
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 13, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries