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P50828

- HEMO_PIG

UniProt

P50828 - HEMO_PIG

Protein

Hemopexin

Gene

HPX

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

    Catalytic activityi

    Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi151 – 1511Iron (heme 1 axial ligand)By similarity
    Metal bindingi237 – 2371Iron (heme 2 axial ligand)By similarity
    Metal bindingi286 – 2861Iron (heme 2 axial ligand)By similarity

    GO - Molecular functioni

    1. hyalurononglucosaminidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemopexin
    Alternative name(s):
    Hyaluronidase (EC:3.2.1.35)
    Gene namesi
    Name:HPX
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 28281 PublicationAdd
    BLAST
    Chaini29 – 459431HemopexinPRO_0000021408Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi52 ↔ 232By similarity
    Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi150 ↔ 155By similarity
    Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi189 ↔ 201By similarity
    Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi250 ↔ 453By similarity
    Disulfide bondi359 ↔ 401By similarity
    Disulfide bondi411 ↔ 428By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP50828.
    PRIDEiP50828.

    Expressioni

    Tissue specificityi

    Expressed by the liver and secreted in plasma.

    Interactioni

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000015556.

    Structurei

    3D structure databases

    ProteinModelPortaliP50828.
    SMRiP50828. Positions 50-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati55 – 9541Hemopexin 1Add
    BLAST
    Repeati96 – 14045Hemopexin 2Add
    BLAST
    Repeati141 – 18545Hemopexin 3Add
    BLAST
    Repeati186 – 23247Hemopexin 4Add
    BLAST
    Repeati252 – 29746Hemopexin 5Add
    BLAST
    Repeati298 – 34548Hemopexin 6Add
    BLAST
    Repeati350 – 38940Hemopexin 7Add
    BLAST
    Repeati393 – 44351Hemopexin 8Add
    BLAST

    Sequence similaritiesi

    Belongs to the hemopexin family.Curated
    Contains 8 hemopexin repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG251805.
    HOGENOMiHOG000112887.
    HOVERGENiHBG005956.

    Family and domain databases

    Gene3Di2.110.10.10. 2 hits.
    InterProiIPR000585. Hemopexin-like_dom.
    IPR018487. Hemopexin-like_repeat.
    IPR016358. Hemopexin_chordata.
    IPR018486. Hemopexin_CS.
    [Graphical view]
    PfamiPF00045. Hemopexin. 3 hits.
    [Graphical view]
    PIRSFiPIRSF002551. Hemopexin_chordata. 1 hit.
    SMARTiSM00120. HX. 5 hits.
    [Graphical view]
    SUPFAMiSSF50923. SSF50923. 2 hits.
    PROSITEiPS00024. HEMOPEXIN. 2 hits.
    PS51642. HEMOPEXIN_2. 8 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50828-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARALGTVEA PWLLGLCCSL AIAHPLSLTA GPKHGAEGRN ESKPDPDVTE    50
    RCSDGWGFDA STLDEHGAML FFKGPSVWAG QNWTRGLISE RWKNAPSSVD 100
    AAFRRGHDRV FLIQGDKVWV YPPEKEKENP RSLQEEFPGV PSPLDAAVEC 150
    HRGECQDEGV LFFQGTHTWF WDSTTKTTKE RLWPAVGNCS SAMRWISRYY 200
    CFRGNQFLRF DPVTGHVDPK YPRDVRDYFM SCPGRGHAHR NATHRGDDRC 250
    SPDLVLTALL SDNHGATYAF RGTHYWRLDT SRDGWHSWPI DHQWSHGPSA 300
    VDAAFSWDDK LYLIQGTQVY IFLTKAGYTL VDNYPKQLEK ELGSPHGISL 350
    DAVDATFVCP GTSRLHVMAG RKLWWLDLSL GAQGPWTELP WPHEKVDAAL 400
    CTEKSLGPNS CSASGLGLYI VHGPHVYCYK DVEKLVSAKA LPQPQSVNSL 450
    LGCHRSRGS 459
    Length:459
    Mass (Da):51,306
    Last modified:October 1, 1996 - v1
    Checksum:iDB06BB44C29789CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14751 mRNA. Translation: AAC48457.1.
    PIRiA55486.
    RefSeqiNP_999118.1. NM_213953.2.
    UniGeneiSsc.4250.

    Genome annotation databases

    GeneIDi396998.
    KEGGissc:396998.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U14751 mRNA. Translation: AAC48457.1 .
    PIRi A55486.
    RefSeqi NP_999118.1. NM_213953.2.
    UniGenei Ssc.4250.

    3D structure databases

    ProteinModelPortali P50828.
    SMRi P50828. Positions 50-453.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000015556.

    Proteomic databases

    PaxDbi P50828.
    PRIDEi P50828.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396998.
    KEGGi ssc:396998.

    Organism-specific databases

    CTDi 3263.

    Phylogenomic databases

    eggNOGi NOG251805.
    HOGENOMi HOG000112887.
    HOVERGENi HBG005956.

    Family and domain databases

    Gene3Di 2.110.10.10. 2 hits.
    InterProi IPR000585. Hemopexin-like_dom.
    IPR018487. Hemopexin-like_repeat.
    IPR016358. Hemopexin_chordata.
    IPR018486. Hemopexin_CS.
    [Graphical view ]
    Pfami PF00045. Hemopexin. 3 hits.
    [Graphical view ]
    PIRSFi PIRSF002551. Hemopexin_chordata. 1 hit.
    SMARTi SM00120. HX. 5 hits.
    [Graphical view ]
    SUPFAMi SSF50923. SSF50923. 2 hits.
    PROSITEi PS00024. HEMOPEXIN. 2 hits.
    PS51642. HEMOPEXIN_2. 8 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a mammalian hyaluronidase reveals identity with hemopexin, a serum heme-binding protein."
      Zhu L., Hope T.J., Hall J., Davies A., Stern M., Mueller-Eberhard U., Stern R., Parslow T.G.
      J. Biol. Chem. 269:32092-32097(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-48 AND 368-388.
      Tissue: Liver.

    Entry informationi

    Entry nameiHEMO_PIG
    AccessioniPrimary (citable) accession number: P50828
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The isolated N-terminal domain binds one heme. The full-length protein also binds one heme, but at a different site. The physiological significance of this is not clear By similarity.By similarity

    Caution

    Lacks the conserved His heme iron ligand in position 81. There is a Gln in this position.Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3