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Protein

Hemopexin

Gene

HPX

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi151 – 1511Iron (heme 1 axial ligand)By similarity
Metal bindingi237 – 2371Iron (heme 2 axial ligand)By similarity
Metal bindingi286 – 2861Iron (heme 2 axial ligand)By similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hemopexin
Alternative name(s):
Hyaluronidase (EC:3.2.1.35)
Gene namesi
Name:HPX
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28281 PublicationAdd
BLAST
Chaini29 – 459431HemopexinPRO_0000021408Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi40 – 401N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi52 ↔ 232By similarity
Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi150 ↔ 155By similarity
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi189 ↔ 201By similarity
Glycosylationi241 – 2411N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi250 ↔ 453By similarity
Disulfide bondi359 ↔ 401By similarity
Disulfide bondi411 ↔ 428By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP50828.
PRIDEiP50828.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015556.

Structurei

3D structure databases

ProteinModelPortaliP50828.
SMRiP50828. Positions 50-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati55 – 9541Hemopexin 1Add
BLAST
Repeati96 – 14045Hemopexin 2Add
BLAST
Repeati141 – 18545Hemopexin 3Add
BLAST
Repeati186 – 23247Hemopexin 4Add
BLAST
Repeati252 – 29746Hemopexin 5Add
BLAST
Repeati298 – 34548Hemopexin 6Add
BLAST
Repeati350 – 38940Hemopexin 7Add
BLAST
Repeati393 – 44351Hemopexin 8Add
BLAST

Sequence similaritiesi

Belongs to the hemopexin family.Curated
Contains 8 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG251805.
HOGENOMiHOG000112887.
HOVERGENiHBG005956.
InParanoidiP50828.
KOiK18977.

Family and domain databases

Gene3Di2.110.10.10. 2 hits.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR016358. Hemopexin_chordata.
IPR018486. Hemopexin_CS.
[Graphical view]
PfamiPF00045. Hemopexin. 3 hits.
[Graphical view]
PIRSFiPIRSF002551. Hemopexin_chordata. 1 hit.
SMARTiSM00120. HX. 5 hits.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 2 hits.
PROSITEiPS00024. HEMOPEXIN. 2 hits.
PS51642. HEMOPEXIN_2. 8 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50828-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARALGTVEA PWLLGLCCSL AIAHPLSLTA GPKHGAEGRN ESKPDPDVTE
60 70 80 90 100
RCSDGWGFDA STLDEHGAML FFKGPSVWAG QNWTRGLISE RWKNAPSSVD
110 120 130 140 150
AAFRRGHDRV FLIQGDKVWV YPPEKEKENP RSLQEEFPGV PSPLDAAVEC
160 170 180 190 200
HRGECQDEGV LFFQGTHTWF WDSTTKTTKE RLWPAVGNCS SAMRWISRYY
210 220 230 240 250
CFRGNQFLRF DPVTGHVDPK YPRDVRDYFM SCPGRGHAHR NATHRGDDRC
260 270 280 290 300
SPDLVLTALL SDNHGATYAF RGTHYWRLDT SRDGWHSWPI DHQWSHGPSA
310 320 330 340 350
VDAAFSWDDK LYLIQGTQVY IFLTKAGYTL VDNYPKQLEK ELGSPHGISL
360 370 380 390 400
DAVDATFVCP GTSRLHVMAG RKLWWLDLSL GAQGPWTELP WPHEKVDAAL
410 420 430 440 450
CTEKSLGPNS CSASGLGLYI VHGPHVYCYK DVEKLVSAKA LPQPQSVNSL

LGCHRSRGS
Length:459
Mass (Da):51,306
Last modified:October 1, 1996 - v1
Checksum:iDB06BB44C29789CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14751 mRNA. Translation: AAC48457.1.
PIRiA55486.
RefSeqiNP_999118.1. NM_213953.2.
UniGeneiSsc.4250.

Genome annotation databases

GeneIDi396998.
KEGGissc:396998.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14751 mRNA. Translation: AAC48457.1.
PIRiA55486.
RefSeqiNP_999118.1. NM_213953.2.
UniGeneiSsc.4250.

3D structure databases

ProteinModelPortaliP50828.
SMRiP50828. Positions 50-453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015556.

Proteomic databases

PaxDbiP50828.
PRIDEiP50828.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396998.
KEGGissc:396998.

Organism-specific databases

CTDi3263.

Phylogenomic databases

eggNOGiNOG251805.
HOGENOMiHOG000112887.
HOVERGENiHBG005956.
InParanoidiP50828.
KOiK18977.

Family and domain databases

Gene3Di2.110.10.10. 2 hits.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR016358. Hemopexin_chordata.
IPR018486. Hemopexin_CS.
[Graphical view]
PfamiPF00045. Hemopexin. 3 hits.
[Graphical view]
PIRSFiPIRSF002551. Hemopexin_chordata. 1 hit.
SMARTiSM00120. HX. 5 hits.
[Graphical view]
SUPFAMiSSF50923. SSF50923. 2 hits.
PROSITEiPS00024. HEMOPEXIN. 2 hits.
PS51642. HEMOPEXIN_2. 8 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of a mammalian hyaluronidase reveals identity with hemopexin, a serum heme-binding protein."
    Zhu L., Hope T.J., Hall J., Davies A., Stern M., Mueller-Eberhard U., Stern R., Parslow T.G.
    J. Biol. Chem. 269:32092-32097(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-48 AND 368-388.
    Tissue: Liver.

Entry informationi

Entry nameiHEMO_PIG
AccessioniPrimary (citable) accession number: P50828
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 29, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The isolated N-terminal domain binds one heme. The full-length protein also binds one heme, but at a different site. The physiological significance of this is not clear (By similarity).By similarity

Caution

Lacks the conserved His heme iron ligand in position 81. There is a Gln in this position.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.