P50828 (HEMO_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hemopexin

Alternative name(s):
Hyaluronidase
EC=3.2.1.35

Gene names

Name:

HPX

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Secreted.
Tissue specificity	Expressed by the liver and secreted in plasma.
Miscellaneous	The isolated N-terminal domain binds one heme. The full-length protein also binds one heme, but at a different site. The physiological significance of this is not clear By similarity.
Sequence similarities	Belongs to the hemopexin family. Contains 5 hemopexin-like domains.
Caution	Lacks the conserved His heme iron ligand in position 81. There is a Gln in this position.

Ontologies

Keywords
Biological process	Transport
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Heme Iron Metal-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Ref.1
	Chain	29 – 459	431	Hemopexin		PRO_0000021408
Regions
	Domain	58 – 97	40	Hemopexin-like 1
	Domain	99 – 142	44	Hemopexin-like 2
	Domain	189 – 232	44	Hemopexin-like 3
	Domain	256 – 299	44	Hemopexin-like 4
	Domain	301 – 346	46	Hemopexin-like 5
Sites
	Metal binding	151	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	237	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	286	1	Iron (heme 2 axial ligand) By similarity
Amino acid modifications
	Glycosylation	40	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Disulfide bond	52 ↔ 232		By similarity
	Disulfide bond	150 ↔ 155		By similarity
	Disulfide bond	189 ↔ 201		By similarity
	Disulfide bond	250 ↔ 453		By similarity
	Disulfide bond	359 ↔ 401		By similarity
	Disulfide bond	411 ↔ 428		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50828 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: DB06BB44C29789CF	FASTA	459	51,306
	10 20 30 40 50 60 MARALGTVEA PWLLGLCCSL AIAHPLSLTA GPKHGAEGRN ESKPDPDVTE RCSDGWGFDA 70 80 90 100 110 120 STLDEHGAML FFKGPSVWAG QNWTRGLISE RWKNAPSSVD AAFRRGHDRV FLIQGDKVWV 130 140 150 160 170 180 YPPEKEKENP RSLQEEFPGV PSPLDAAVEC HRGECQDEGV LFFQGTHTWF WDSTTKTTKE 190 200 210 220 230 240 RLWPAVGNCS SAMRWISRYY CFRGNQFLRF DPVTGHVDPK YPRDVRDYFM SCPGRGHAHR 250 260 270 280 290 300 NATHRGDDRC SPDLVLTALL SDNHGATYAF RGTHYWRLDT SRDGWHSWPI DHQWSHGPSA 310 320 330 340 350 360 VDAAFSWDDK LYLIQGTQVY IFLTKAGYTL VDNYPKQLEK ELGSPHGISL DAVDATFVCP 370 380 390 400 410 420 GTSRLHVMAG RKLWWLDLSL GAQGPWTELP WPHEKVDAAL CTEKSLGPNS CSASGLGLYI 430 440 450 VHGPHVYCYK DVEKLVSAKA LPQPQSVNSL LGCHRSRGS

References

[1]	"Molecular cloning of a mammalian hyaluronidase reveals identity with hemopexin, a serum heme-binding protein." Zhu L., Hope T.J., Hall J., Davies A., Stern M., Mueller-Eberhard U., Stern R., Parslow T.G. J. Biol. Chem. 269:32092-32097(1994) [PubMed: 7798203] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-48 AND 368-388. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U14751 mRNA. Translation: AAC48457.1.
PIR	A55486.
RefSeq	NP_999118.1. NM_213953.1.
UniGene	Ssc.4250.
3D structure databases
ProteinModelPortal	P50828.
SMR	P50828. Positions 50-453.
ModBase	Search...
Protein-protein interaction databases
STRING	P50828.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396998.
KEGG	ssc:396998.
Organism-specific databases
CTD	3263.
Phylogenomic databases
GeneTree	ENSGT00390000009178.
HOVERGEN	HBG005956.
OrthoDB	EOG4R502N.
Family and domain databases
InterPro	IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR016358. Hemopexin_chordata. [Graphical view]
Gene3D	G3DSA:2.110.10.10. Hemopexin. 2 hits.
Pfam	PF00045. Hemopexin. 4 hits. [Graphical view]
PIRSF	PIRSF002551. Hemopexin_chordata. 1 hit.
SMART	SM00120. HX. 5 hits. [Graphical view]
SUPFAM	SSF50923. Hemopexin. 2 hits.
PROSITE	PS00024. HEMOPEXIN. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMO_PIG

Accession

Primary (citable) accession number: P50828

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	July 27, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program