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Protein

Regulatory protein E2

Gene

E2

Organism
Human papillomavirus type 21
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processDNA replication, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein E2UniRule annotation
Gene namesi
Name:E2UniRule annotation
OrganismiHuman papillomavirus type 21
Taxonomic identifieri31548 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeBetapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009165 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001332001 – 503Regulatory protein E2Add BLAST503

Post-translational modificationi

Phosphorylated.UniRule annotation

Keywords - PTMi

Phosphoprotein

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Binds DNA as homodimer. Interacts with protein E1; this interaction greatly increases E1 DNA-binding activity. Interacts with protein L1; this interaction enhances E2-dependent replication and transcription activation. Interacts with protein L2; this interaction inhibits E2 transcriptional activity but not DNA replication function E2. Interacts with protein E7; this interaction inhibits E7 oncogenic activity. Interacts with host TAF1; this interaction modulates E2-dependent transcriptional regulation. Interacts with host BRD4; this interaction mediates E2 transcriptional activation function. Additionally, the interaction with host BRD4 on mitotic chromosomes mediates tethering of the viral genome. Interacts with host TOPBP1; this interaction is required for optimal viral DNA replication.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP50767.
SMRiP50767.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 201Transactivation domainUniRule annotationAdd BLAST201
Regioni419 – 503DNA-binding domainUniRule annotationAdd BLAST85

Sequence similaritiesi

Belongs to the papillomaviridae E2 protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900010D.

Family and domain databases

HAMAPiMF_04001. PPV_E2. 1 hit.
InterProiView protein in InterPro
IPR035975. E2/EBNA1_C_sf.
IPR000427. Papillomavirus_E2_C.
IPR001866. Papillomavirus_E2_N.
IPR033668. Reg_prot_E2.
IPR036050. Regulatory_protein_E2_N.
PfamiView protein in Pfam
PF00511. PPV_E2_C. 1 hit.
PF00508. PPV_E2_N. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000672. E2_early_recog_prot_C. 1 hit.
SUPFAMiSSF51332. SSF51332. 1 hit.
SSF54957. SSF54957. 1 hit.

Sequencei

Sequence statusi: Complete.

P50767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENLSDRFNV LQDQLMNIYE SAANTIESQI EHWQTLRKEA VLLYFARQKG
60 70 80 90 100
VTRLGYQYVP PLAVSESRAK QAIGMMLQLQ SLQKSEYAKE PWSLVDTSAE
110 120 130 140 150
TFRSPPENHF KKGPVSVEVI YDNDKDNANA YTMWRYVYYV DDDDQWHKSP
160 170 180 190 200
SGVNHTGIYF MQGTFRHYYV LFADDASRYS RTGHWEVNVN KETVFAPVTS
210 220 230 240 250
STPPDSPGGQ ADSNTSSTTP ATTTDSTSRL SSTRKQSQQT NTKGRRYGRR
260 270 280 290 300
PSSRTRRTTQ THQRRRSRSK SRSRSRSRSR LRSRSRSRSR SYSRSRSQSS
310 320 330 340 350
DQPQYRFRSG GQVSLITTAT TTTTTATNYS TRGSGRGSSS TSSSTSKRPR
360 370 380 390 400
RPRGGAIGGS SGRGRRSSST SPSPSKRSRG KSESVRQRGI SPDDVGKSLQ
410 420 430 440 450
SVSTRNTGRL GRLLDEALDP PVILVRGEPN TLKCFRNRAK LKYAGLYKAF
460 470 480 490 500
STAWSWVAGD GTERLGRSRM LISFFSFEQR KDFDKTVKYP KGVDRSYGSF

DSL
Length:503
Mass (Da):56,751
Last modified:October 1, 1996 - v1
Checksum:iAE72A9DF63367266
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31779 Genomic DNA. Translation: AAA79397.1.

Similar proteinsi

Entry informationi

Entry nameiVE2_HPV21
AccessioniPrimary (citable) accession number: P50767
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families