Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Replication protein E1

Gene

E1

Organism
Human papillomavirus type 24
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi435 – 442ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein E1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase E1UniRule annotation
Gene namesi
Name:E1UniRule annotation
OrganismiHuman papillomavirus type 24
Taxonomic identifieri37956 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeBetapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000158064 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001331221 – 607Replication protein E1Add BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81Phosphoserine; by hostUniRule annotation1
Modified residuei89Phosphoserine; by hostUniRule annotation1

Post-translational modificationi

Phosphorylated.UniRule annotation

Keywords - PTMi

Phosphoprotein

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP50762.
SMRiP50762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini409 – 559SF3 helicaseUniRule annotationAdd BLAST151

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 310DNA-binding regionUniRule annotationAdd BLAST164

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 78Nuclear localization signalUniRule annotation3
Motifi88 – 97Nuclear export signalUniRule annotation10

Sequence similaritiesi

Belongs to the papillomaviridae E1 protein family.UniRule annotation

Family and domain databases

HAMAPiMF_04000. PPV_E1. 1 hit.
InterProiView protein in InterPro
IPR001177. DNA_helicase_E1_C_Papillomavir.
IPR014000. DNA_helicase_E1_N_Papillomavir.
IPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR016393. Rep_E1_papillomaV.
PfamiView protein in Pfam
PF00519. PPV_E1_C. 1 hit.
PF00524. PPV_E1_N. 1 hit.
PIRSFiPIRSF003383. Rep_E1_papillomaV. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51206. SF3_HELICASE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P50762-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNKGSETD GLHEWCLLEA DCSDIENDLD TLLEQNSDSD VSDLISNDGD
60 70 80 90 100
LEQGNSRELF QQQELEESNA LLQSLKRKYI SPKAVLQLSP QLESISLSSD
110 120 130 140 150
HKTKRKLFAE QDSGVELTLT NETEDVTTLV QQEEEVPALE TSSTSNLRKE
160 170 180 190 200
DNAHYKELMR CSNLKATLLS KFKNAFGVSF VELTRQFRSN KTCCNDWVVA
210 220 230 240 250
IYGVNYDLFE SSKQLLQQHC DYIWVTEMSA MFLYLLCFKA GKNRQTVIRL
260 270 280 290 300
LVSMLYVAEE QILSEPPKLR STVSALFWYK GSSNAATFTH GSYPKWIIEQ
310 320 330 340 350
TLIGHQTGEA ATFDMSTMVQ WAFDNDLTEE ADIAFQYAKL APDDVNATAW
360 370 380 390 400
LAHNNQARFV RECANMVRYY KKGQMREMSM SAWIHFKLEQ VEGEGQWSTI
410 420 430 440 450
VKFIRYQGIN FISFLSALKD FLHGKPKKNC LLIYGPPNTG KSAFTMSLIK
460 470 480 490 500
VLHGRVISFV NSKSHFWLQP MSEAKIALLD DATDPCWIYM DTYLRNGLDG
510 520 530 540 550
HLVSLDCKHK APIQIRFPPL LITSNINAMA EPNYRYLHSR LVAFEFPNPF
560 570 580 590 600
PMKDDDTPEF ELTDQSWKSF FKRLWRQLDL SDQEDEGEDG EPEKAFRCVA

RSTNEHL
Length:607
Mass (Da):69,547
Last modified:October 1, 1996 - v1
Checksum:i1B6C4F416CA310CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31782 Genomic DNA. Translation: AAA79417.1.

Similar proteinsi

Entry informationi

Entry nameiVE1_HPV24
AccessioniPrimary (citable) accession number: P50762
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 12, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families