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Protein

Replication protein E1

Gene

E1

Organism
Human papillomavirus type 22
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi436 – 443ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein E1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase E1UniRule annotation
Gene namesi
Name:E1UniRule annotation
OrganismiHuman papillomavirus type 22
Taxonomic identifieri37954 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeBetapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009111 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001331201 – 608Replication protein E1Add BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88Phosphoserine; by hostUniRule annotation1
Modified residuei96Phosphoserine; by hostUniRule annotation1
Cross-linki517Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation

Post-translational modificationi

Phosphorylated.UniRule annotation
Sumoylated.UniRule annotation

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP50760.
SMRiP50760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini410 – 560SF3 helicaseUniRule annotationAdd BLAST151

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni148 – 311DNA-binding regionUniRule annotationAdd BLAST164

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi83 – 85Nuclear localization signalUniRule annotation3

Sequence similaritiesi

Belongs to the papillomaviridae E1 protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000HF.

Family and domain databases

HAMAPiMF_04000. PPV_E1. 1 hit.
InterProiView protein in InterPro
IPR001177. DNA_helicase_E1_C_Papillomavir.
IPR014000. DNA_helicase_E1_N_Papillomavir.
IPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR016393. Rep_E1_papillomaV.
PfamiView protein in Pfam
PF00519. PPV_E1_C. 1 hit.
PF00524. PPV_E1_N. 1 hit.
PIRSFiPIRSF003383. Rep_E1_papillomaV. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51206. SF3_HELICASE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P50760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDDKGTDTT DAKEGCSGWF MLEAACSDDS DLDNSLEKLF EDGTESDVSD
60 70 80 90 100
LINDDDTAAQ GNSRELLCQQ QSEECEQQIQ YLKRKYFSPK AVQQLSPRLQ
110 120 130 140 150
SMNISPGHKS KRRLFVEHDS GLECSLNEAE DLTEEVEVPA SAPAPAAQGG
160 170 180 190 200
VGSGHYTSLL RCNNVKAVLL GKFKDAFGVS YNELTRQFRS NKTCCKHWVL
210 220 230 240 250
AIYAAKDELI DASKQLLQQH CTYLWLQTFS PMSLYLCCFN VGKSRETVMR
260 270 280 290 300
LLSSMLQVNE NHILSEPPKI RSMIAALFWY KGSMNPNVYA FGEYPEWIMT
310 320 330 340 350
QTMIHHQTAD SVQFDLSEMI QWAYDQDYVD ECTIAYQYAR LADSNSNARA
360 370 380 390 400
FLAHNSQAKY VRECAQMVRY YKRGEMRDMS ISAWIHHCIS KIEGDGHWQD
410 420 430 440 450
IVKFLRYQGL NFIVFLDKFR TFLKNFPKKN CLLICGPPDT GKSMFSMSLM
460 470 480 490 500
KALRGQVVSF ANSKSHFWLQ PLADAKLALL DDATEVCWQY IDAFLRNGLD
510 520 530 540 550
GNMVSLDMKH RAPCQMKFPP LIITSNISLK KEKKFPYLHS RIYEFEFPNK
560 570 580 590 600
FPFDANDTPL FKLTDQSWAS FFKRLWTQLE LSDQEEEGEN GETQRTFQCT

TREVNGLI
Length:608
Mass (Da):69,698
Last modified:October 1, 1996 - v1
Checksum:i0206805111ECA17D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31780 Genomic DNA. Translation: AAA79403.1.

Similar proteinsi

Entry informationi

Entry nameiVE1_HPV22
AccessioniPrimary (citable) accession number: P50760
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 12, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families