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Protein

Troponin T, cardiac muscle

Gene

Tnnt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • ATPase activity Source: Ensembl
  • protein binding, bridging Source: RGD
  • tropomyosin binding Source: UniProtKB
  • troponin C binding Source: UniProtKB
  • troponin I binding Source: RGD

GO - Biological processi

  • actin crosslink formation Source: RGD
  • cardiac muscle contraction Source: RGD
  • muscle contraction Source: RGD
  • muscle filament sliding Source: UniProtKB
  • negative regulation of ATPase activity Source: UniProtKB
  • positive regulation of ATPase activity Source: UniProtKB
  • protein heterooligomerization Source: RGD
  • regulation of heart contraction Source: UniProtKB
  • regulation of muscle contraction Source: RGD
  • regulation of muscle filament sliding speed Source: BHF-UCL
  • response to calcium ion Source: UniProtKB
  • ventricular cardiac muscle tissue morphogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Enzyme and pathway databases

ReactomeiR-RNO-390522. Striated Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin T, cardiac muscle
Short name:
TnTc
Alternative name(s):
Cardiac muscle troponin T
Short name:
cTnT
Gene namesi
Name:Tnnt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi3882. Tnnt2.

Subcellular locationi

GO - Cellular componenti

  • myofibril Source: RGD
  • striated muscle thin filament Source: UniProtKB
  • troponin complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 299298Troponin T, cardiac musclePRO_0000186176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineCombined sources
Modified residuei191 – 1911PhosphoserineBy similarity
Modified residuei205 – 2051Phosphothreonine; by PKC/PRKCABy similarity
Modified residuei209 – 2091Phosphoserine; by PKC/PRKCABy similarity
Modified residuei214 – 2141Phosphothreonine; by PKC/PRKCA and RAF11 Publication
Modified residuei295 – 2951Phosphothreonine; by PKC/PRKCABy similarity
Isoform 2 (identifier: P50753-2)
Modified residuei2 – 21PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation at Thr-214 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP50753.
PRIDEiP50753.

PTM databases

iPTMnetiP50753.
PhosphoSiteiP50753.

Expressioni

Gene expression databases

ExpressionAtlasiP50753. baseline and differential.
GenevisibleiP50753. RN.

Interactioni

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • protein binding, bridging Source: RGD
  • tropomyosin binding Source: UniProtKB
  • troponin C binding Source: UniProtKB
  • troponin I binding Source: RGD

Protein-protein interaction databases

BioGridi246955. 2 interactions.
STRINGi10116.ENSRNOP00000049297.

Structurei

3D structure databases

ProteinModelPortaliP50753.
SMRiP50753. Positions 102-135, 211-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the troponin T family.Curated

Phylogenomic databases

eggNOGiKOG3634. Eukaryota.
ENOG410XS6A. LUCA.
GeneTreeiENSGT00390000013611.
HOGENOMiHOG000231049.
HOVERGENiHBG052790.
InParanoidiP50753.
KOiK12045.
OMAiELWEWMY.
OrthoDBiEOG091G0U67.

Family and domain databases

InterProiIPR027707. TNNT.
IPR001978. Troponin.
[Graphical view]
PANTHERiPTHR11521. PTHR11521. 1 hit.
PfamiPF00992. Troponin. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P50753-1) [UniParc]FASTAAdd to basket
Also known as: Embryonic

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDAEEEVVE YEEEQEEEDW SEEEEDEQEE AVEEEDGEAE PDPEGEAEAE
60 70 80 90 100
EDKAEEVGPD EEARDAEDGP VEDSKPKPSR LFMPNLVPPK IPDGERVDFD
110 120 130 140 150
DIHRKRMEKD LNELQTLIEA HFENRKKEEE ELISLKDRIE KRRAERAEQQ
160 170 180 190 200
RIRNEREKER QNRLAEERAR REEEENRRKA EDEARKKKAL SNMMHFGGYI
210 220 230 240 250
QKAQTERKSG KRQTEREKKK KILAERRKVL AIDHLNEDQL REKAKELWQS
260 270 280 290
IHNLEAEKFD LQEKFKQQKY EINVLRNRIN DNQKVSKTRG KAKVTGRWK
Length:299
Mass (Da):35,730
Last modified:January 23, 2007 - v2
Checksum:iB6042E2AD53B30B5
GO
Isoform 2 (identifier: P50753-2) [UniParc]FASTAAdd to basket
Also known as: Adult

The sequence of this isoform differs from the canonical sequence as follows:
     18-27: Missing.

Show »
Length:289
Mass (Da):34,452
Checksum:i47B2A588E82AE6FD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti205 – 2051T → A in AAB07676 (PubMed:1433301).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei18 – 2710Missing in isoform 2. CuratedVSP_006657

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26051 mRNA. Translation: AAA42296.1.
M26052 mRNA. Translation: AAA42297.1.
M80829 Genomic DNA. Translation: AAB07676.1.
PIRiA44781.
B44781.
S30443.
RefSeqiNP_036808.1. NM_012676.1. [P50753-1]
XP_006249900.1. XM_006249838.1. [P50753-2]
XP_008767759.1. XM_008769537.1. [P50753-1]
UniGeneiRn.9965.

Genome annotation databases

EnsembliENSRNOT00000050284; ENSRNOP00000041541; ENSRNOG00000033734. [P50753-2]
ENSRNOT00000084986; ENSRNOP00000069434; ENSRNOG00000033734. [P50753-1]
GeneIDi24837.
KEGGirno:24837.
UCSCiRGD:3882. rat. [P50753-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26051 mRNA. Translation: AAA42296.1.
M26052 mRNA. Translation: AAA42297.1.
M80829 Genomic DNA. Translation: AAB07676.1.
PIRiA44781.
B44781.
S30443.
RefSeqiNP_036808.1. NM_012676.1. [P50753-1]
XP_006249900.1. XM_006249838.1. [P50753-2]
XP_008767759.1. XM_008769537.1. [P50753-1]
UniGeneiRn.9965.

3D structure databases

ProteinModelPortaliP50753.
SMRiP50753. Positions 102-135, 211-285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246955. 2 interactions.
STRINGi10116.ENSRNOP00000049297.

PTM databases

iPTMnetiP50753.
PhosphoSiteiP50753.

Proteomic databases

PaxDbiP50753.
PRIDEiP50753.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000050284; ENSRNOP00000041541; ENSRNOG00000033734. [P50753-2]
ENSRNOT00000084986; ENSRNOP00000069434; ENSRNOG00000033734. [P50753-1]
GeneIDi24837.
KEGGirno:24837.
UCSCiRGD:3882. rat. [P50753-1]

Organism-specific databases

CTDi7139.
RGDi3882. Tnnt2.

Phylogenomic databases

eggNOGiKOG3634. Eukaryota.
ENOG410XS6A. LUCA.
GeneTreeiENSGT00390000013611.
HOGENOMiHOG000231049.
HOVERGENiHBG052790.
InParanoidiP50753.
KOiK12045.
OMAiELWEWMY.
OrthoDBiEOG091G0U67.

Enzyme and pathway databases

ReactomeiR-RNO-390522. Striated Muscle Contraction.

Miscellaneous databases

PROiP50753.

Gene expression databases

ExpressionAtlasiP50753. baseline and differential.
GenevisibleiP50753. RN.

Family and domain databases

InterProiIPR027707. TNNT.
IPR001978. Troponin.
[Graphical view]
PANTHERiPTHR11521. PTHR11521. 1 hit.
PfamiPF00992. Troponin. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTNNT2_RAT
AccessioniPrimary (citable) accession number: P50753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.