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Protein

Troponin T, cardiac muscle

Gene

Tnnt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

GO - Molecular functioni

  1. actin binding Source: UniProtKB
  2. structural constituent of cytoskeleton Source: MGI
  3. tropomyosin binding Source: UniProtKB
  4. troponin C binding Source: UniProtKB
  5. troponin I binding Source: UniProtKB

GO - Biological processi

  1. atrial cardiac muscle tissue morphogenesis Source: MGI
  2. heart development Source: MGI
  3. metabolic process Source: MGI
  4. muscle contraction Source: MGI
  5. muscle filament sliding Source: UniProtKB
  6. negative regulation of ATPase activity Source: UniProtKB
  7. positive regulation of ATPase activity Source: UniProtKB
  8. regulation of heart contraction Source: MGI
  9. regulation of muscle contraction Source: InterPro
  10. response to calcium ion Source: UniProtKB
  11. sarcomere organization Source: MGI
  12. ventricular cardiac muscle tissue morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Enzyme and pathway databases

ReactomeiREACT_324616. Striated Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin T, cardiac muscle
Short name:
TnTc
Alternative name(s):
Cardiac muscle troponin T
Short name:
cTnT
Gene namesi
Name:Tnnt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:104597. Tnnt2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. myofibril Source: MGI
  3. sarcomere Source: MGI
  4. striated muscle thin filament Source: UniProtKB
  5. troponin complex Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 301300Troponin T, cardiac musclePRO_0000186174Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21Phosphoserine; by CK2By similarity
Modified residuei207 – 2071Phosphothreonine; by PKC/PRKCA1 Publication
Modified residuei211 – 2111Phosphoserine; by PKC/PRKCA1 Publication
Modified residuei216 – 2161Phosphothreonine; by PKC/PRKCA and RAF11 Publication
Modified residuei297 – 2971Phosphothreonine; by PKC/PRKCA1 Publication

Post-translational modificationi

Phosphorylation at Thr-216 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP50752.
PaxDbiP50752.
PRIDEiP50752.

PTM databases

PhosphoSiteiP50752.

Miscellaneous databases

PMAP-CutDBP50752.

Expressioni

Gene expression databases

BgeeiP50752.
CleanExiMM_TNNT2.
ExpressionAtlasiP50752. baseline and differential.
GenevestigatoriP50752.

Interactioni

Protein-protein interaction databases

IntActiP50752. 3 interactions.
MINTiMINT-4138660.

Structurei

3D structure databases

ProteinModelPortaliP50752.
SMRiP50752. Positions 213-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the troponin T family.Curated

Phylogenomic databases

eggNOGiNOG299534.
GeneTreeiENSGT00390000013611.
HOGENOMiHOG000231049.
HOVERGENiHBG052790.
InParanoidiP50752.
KOiK12045.

Family and domain databases

InterProiIPR027707. TNNT.
IPR001978. Troponin.
[Graphical view]
PANTHERiPTHR11521. PTHR11521. 1 hit.
PfamiPF00992. Troponin. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Embryonic (identifier: P50752-1) [UniParc]FASTAAdd to basket

Also known as: EA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDAEEVVEE YEEEQEEEDW SEEEEDEQEE AVEEEEAGGA EPEPEGEAET
60 70 80 90 100
EEANVEEVGP DEEAKDAEEG PVEDTKPKPS RLFMPNLVPP KIPDGERVDF
110 120 130 140 150
DDIHRKRVEK DLNELQTLIE AHFENRKKEE EELISLKDRI EKRRAERAEQ
160 170 180 190 200
QRIRNEREKE RQNRLAEERA RREEEENRRK AEDEARKKKA LSNMMHFGGY
210 220 230 240 250
IQKQAQTERK SGKRQTEREK KKKILAERRK ALAIDHLNED QLREKAKELW
260 270 280 290 300
QSIHNLEAEK FDLQEKFKQQ KYEINVLRNR INDNQKVSKT RGKAKVTGRW

K
Length:301
Mass (Da):35,825
Last modified:January 22, 2007 - v2
Checksum:i60B2185A140AF2D5
GO
Isoform A1 (identifier: P50752-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-26: EDWSEEEED → AVE

Show »
Length:295
Mass (Da):34,976
Checksum:i4B4C7ED7459289D5
GO
Isoform A2 (identifier: P50752-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-27: Missing.

Show »
Length:291
Mass (Da):34,547
Checksum:i9BDA2958F567B11F
GO
Isoform A3B (identifier: P50752-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-27: Missing.
     204-206: Missing.

Show »
Length:288
Mass (Da):34,220
Checksum:i002769E8DC4715B1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei18 – 2710Missing in isoform A2 and isoform A3B. CuratedVSP_006649
Alternative sequencei18 – 269EDWSEEEED → AVE in isoform A1. CuratedVSP_007913
Alternative sequencei204 – 2063Missing in isoform A3B. CuratedVSP_006650

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47549 mRNA. Translation: AAA85345.1.
L47550 mRNA. Translation: AAA85346.1.
L47551 mRNA. Translation: AAA85347.1.
L47552 mRNA. Translation: AAA85348.1.
L47553 mRNA. Translation: AAA85349.1.
L47570 Genomic DNA. Translation: AAA85350.1.
L47599 mRNA. Translation: AAA85351.1.
L47600 mRNA. Translation: AAA85352.1.
CCDSiCCDS48376.1. [P50752-4]
CCDS48377.1. [P50752-1]
CCDS48378.1. [P50752-3]
RefSeqiNP_001123648.1. NM_001130176.1. [P50752-1]
NP_001123650.1. NM_001130178.2. [P50752-4]
NP_001123651.4. NM_001130179.2.
NP_001123652.1. NM_001130180.2. [P50752-3]
NP_001123653.4. NM_001130181.2.
NP_035749.1. NM_011619.3. [P50752-2]
XP_006529456.1. XM_006529393.2. [P50752-1]
XP_006529457.1. XM_006529394.2. [P50752-1]
XP_006529458.1. XM_006529395.2. [P50752-3]
UniGeneiMm.247470.

Genome annotation databases

EnsembliENSMUST00000027671; ENSMUSP00000027671; ENSMUSG00000026414. [P50752-3]
ENSMUST00000112087; ENSMUSP00000107717; ENSMUSG00000026414. [P50752-1]
ENSMUST00000178854; ENSMUSP00000136265; ENSMUSG00000026414. [P50752-4]
ENSMUST00000189355; ENSMUSP00000139919; ENSMUSG00000026414. [P50752-3]
ENSMUST00000189732; ENSMUSP00000139669; ENSMUSG00000026414. [P50752-4]
GeneIDi21956.
KEGGimmu:21956.
UCSCiuc007ctu.2. mouse. [P50752-4]
uc007ctw.2. mouse. [P50752-3]
uc007ctx.2. mouse. [P50752-2]
uc011wso.1. mouse. [P50752-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L47549 mRNA. Translation: AAA85345.1.
L47550 mRNA. Translation: AAA85346.1.
L47551 mRNA. Translation: AAA85347.1.
L47552 mRNA. Translation: AAA85348.1.
L47553 mRNA. Translation: AAA85349.1.
L47570 Genomic DNA. Translation: AAA85350.1.
L47599 mRNA. Translation: AAA85351.1.
L47600 mRNA. Translation: AAA85352.1.
CCDSiCCDS48376.1. [P50752-4]
CCDS48377.1. [P50752-1]
CCDS48378.1. [P50752-3]
RefSeqiNP_001123648.1. NM_001130176.1. [P50752-1]
NP_001123650.1. NM_001130178.2. [P50752-4]
NP_001123651.4. NM_001130179.2.
NP_001123652.1. NM_001130180.2. [P50752-3]
NP_001123653.4. NM_001130181.2.
NP_035749.1. NM_011619.3. [P50752-2]
XP_006529456.1. XM_006529393.2. [P50752-1]
XP_006529457.1. XM_006529394.2. [P50752-1]
XP_006529458.1. XM_006529395.2. [P50752-3]
UniGeneiMm.247470.

3D structure databases

ProteinModelPortaliP50752.
SMRiP50752. Positions 213-287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP50752. 3 interactions.
MINTiMINT-4138660.

PTM databases

PhosphoSiteiP50752.

Proteomic databases

MaxQBiP50752.
PaxDbiP50752.
PRIDEiP50752.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027671; ENSMUSP00000027671; ENSMUSG00000026414. [P50752-3]
ENSMUST00000112087; ENSMUSP00000107717; ENSMUSG00000026414. [P50752-1]
ENSMUST00000178854; ENSMUSP00000136265; ENSMUSG00000026414. [P50752-4]
ENSMUST00000189355; ENSMUSP00000139919; ENSMUSG00000026414. [P50752-3]
ENSMUST00000189732; ENSMUSP00000139669; ENSMUSG00000026414. [P50752-4]
GeneIDi21956.
KEGGimmu:21956.
UCSCiuc007ctu.2. mouse. [P50752-4]
uc007ctw.2. mouse. [P50752-3]
uc007ctx.2. mouse. [P50752-2]
uc011wso.1. mouse. [P50752-1]

Organism-specific databases

CTDi7139.
MGIiMGI:104597. Tnnt2.

Phylogenomic databases

eggNOGiNOG299534.
GeneTreeiENSGT00390000013611.
HOGENOMiHOG000231049.
HOVERGENiHBG052790.
InParanoidiP50752.
KOiK12045.

Enzyme and pathway databases

ReactomeiREACT_324616. Striated Muscle Contraction.

Miscellaneous databases

ChiTaRSiTnnt2. mouse.
NextBioi301636.
PMAP-CutDBP50752.
PROiP50752.
SOURCEiSearch...

Gene expression databases

BgeeiP50752.
CleanExiMM_TNNT2.
ExpressionAtlasiP50752. baseline and differential.
GenevestigatoriP50752.

Family and domain databases

InterProiIPR027707. TNNT.
IPR001978. Troponin.
[Graphical view]
PANTHERiPTHR11521. PTHR11521. 1 hit.
PfamiPF00992. Troponin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of mouse cardiac troponin T isoforms: characterization of a large sample of independent cDNA clones."
    Jin J.-P., Wang J., Zhang J.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS A1; A2; A3B AND EMBRYONIC).
    Tissue: Heart.
  2. "Identification of a functionally critical protein kinase C phosphorylation residue of cardiac troponin T."
    Sumandea M.P., Pyle W.G., Kobayashi T., de Tombe P.P., Solaro R.J.
    J. Biol. Chem. 278:35135-35144(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-207; SER-211; THR-216 AND THR-297.

Entry informationi

Entry nameiTNNT2_MOUSE
AccessioniPrimary (citable) accession number: P50752
Secondary accession number(s): Q64360, Q64377
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.