Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P50752

- TNNT2_MOUSE

UniProt

P50752 - TNNT2_MOUSE

Protein

Troponin T, cardiac muscle

Gene

Tnnt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

    GO - Molecular functioni

    1. actin binding Source: UniProtKB
    2. structural constituent of cytoskeleton Source: MGI
    3. tropomyosin binding Source: UniProtKB
    4. troponin C binding Source: UniProtKB
    5. troponin I binding Source: UniProtKB

    GO - Biological processi

    1. atrial cardiac muscle tissue morphogenesis Source: MGI
    2. heart development Source: MGI
    3. muscle contraction Source: MGI
    4. muscle filament sliding Source: UniProtKB
    5. negative regulation of ATPase activity Source: UniProtKB
    6. positive regulation of ATPase activity Source: UniProtKB
    7. regulation of heart contraction Source: MGI
    8. regulation of muscle contraction Source: InterPro
    9. response to calcium ion Source: UniProtKB
    10. sarcomere organization Source: MGI
    11. ventricular cardiac muscle tissue morphogenesis Source: MGI

    Keywords - Molecular functioni

    Muscle protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Troponin T, cardiac muscle
    Short name:
    TnTc
    Alternative name(s):
    Cardiac muscle troponin T
    Short name:
    cTnT
    Gene namesi
    Name:Tnnt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:104597. Tnnt2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. myofibril Source: MGI
    3. sarcomere Source: MGI
    4. striated muscle thin filament Source: UniProtKB
    5. troponin complex Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 301300Troponin T, cardiac musclePRO_0000186174Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21Phosphoserine; by CK2By similarity
    Modified residuei207 – 2071Phosphothreonine; by PKC/PRKCA1 Publication
    Modified residuei211 – 2111Phosphoserine; by PKC/PRKCA1 Publication
    Modified residuei216 – 2161Phosphothreonine; by PKC/PRKCA and RAF11 Publication
    Modified residuei297 – 2971Phosphothreonine; by PKC/PRKCA1 Publication

    Post-translational modificationi

    Phosphorylation at Thr-216 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP50752.
    PaxDbiP50752.
    PRIDEiP50752.

    PTM databases

    PhosphoSiteiP50752.

    Miscellaneous databases

    PMAP-CutDBP50752.

    Expressioni

    Gene expression databases

    ArrayExpressiP50752.
    BgeeiP50752.
    CleanExiMM_TNNT2.
    GenevestigatoriP50752.

    Interactioni

    Protein-protein interaction databases

    IntActiP50752. 3 interactions.
    MINTiMINT-4138660.

    Structurei

    3D structure databases

    ProteinModelPortaliP50752.
    SMRiP50752. Positions 213-287.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the troponin T family.Curated

    Phylogenomic databases

    eggNOGiNOG299534.
    GeneTreeiENSGT00390000013611.
    HOGENOMiHOG000231049.
    HOVERGENiHBG052790.
    KOiK12045.

    Family and domain databases

    InterProiIPR027707. TNNT.
    IPR001978. Troponin.
    [Graphical view]
    PANTHERiPTHR11521. PTHR11521. 1 hit.
    PfamiPF00992. Troponin. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform Embryonic (identifier: P50752-1) [UniParc]FASTAAdd to Basket

    Also known as: EA

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSDAEEVVEE YEEEQEEEDW SEEEEDEQEE AVEEEEAGGA EPEPEGEAET    50
    EEANVEEVGP DEEAKDAEEG PVEDTKPKPS RLFMPNLVPP KIPDGERVDF 100
    DDIHRKRVEK DLNELQTLIE AHFENRKKEE EELISLKDRI EKRRAERAEQ 150
    QRIRNEREKE RQNRLAEERA RREEEENRRK AEDEARKKKA LSNMMHFGGY 200
    IQKQAQTERK SGKRQTEREK KKKILAERRK ALAIDHLNED QLREKAKELW 250
    QSIHNLEAEK FDLQEKFKQQ KYEINVLRNR INDNQKVSKT RGKAKVTGRW 300
    K 301
    Length:301
    Mass (Da):35,825
    Last modified:January 23, 2007 - v2
    Checksum:i60B2185A140AF2D5
    GO
    Isoform A1 (identifier: P50752-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         18-26: EDWSEEEED → AVE

    Show »
    Length:295
    Mass (Da):34,976
    Checksum:i4B4C7ED7459289D5
    GO
    Isoform A2 (identifier: P50752-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         18-27: Missing.

    Show »
    Length:291
    Mass (Da):34,547
    Checksum:i9BDA2958F567B11F
    GO
    Isoform A3B (identifier: P50752-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         18-27: Missing.
         204-206: Missing.

    Show »
    Length:288
    Mass (Da):34,220
    Checksum:i002769E8DC4715B1
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei18 – 2710Missing in isoform A2 and isoform A3B. CuratedVSP_006649
    Alternative sequencei18 – 269EDWSEEEED → AVE in isoform A1. CuratedVSP_007913
    Alternative sequencei204 – 2063Missing in isoform A3B. CuratedVSP_006650

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47549 mRNA. Translation: AAA85345.1.
    L47550 mRNA. Translation: AAA85346.1.
    L47551 mRNA. Translation: AAA85347.1.
    L47552 mRNA. Translation: AAA85348.1.
    L47553 mRNA. Translation: AAA85349.1.
    L47570 Genomic DNA. Translation: AAA85350.1.
    L47599 mRNA. Translation: AAA85351.1.
    L47600 mRNA. Translation: AAA85352.1.
    CCDSiCCDS48376.1. [P50752-4]
    CCDS48377.1. [P50752-1]
    CCDS48378.1. [P50752-3]
    RefSeqiNP_001123648.1. NM_001130176.1. [P50752-1]
    NP_001123650.1. NM_001130178.2. [P50752-4]
    NP_001123651.4. NM_001130179.2.
    NP_001123652.1. NM_001130180.2. [P50752-3]
    NP_001123653.4. NM_001130181.2.
    NP_035749.1. NM_011619.3. [P50752-2]
    XP_006529456.1. XM_006529393.1. [P50752-1]
    XP_006529457.1. XM_006529394.1. [P50752-1]
    XP_006529458.1. XM_006529395.1. [P50752-3]
    UniGeneiMm.247470.

    Genome annotation databases

    EnsembliENSMUST00000027671; ENSMUSP00000027671; ENSMUSG00000026414. [P50752-3]
    ENSMUST00000112087; ENSMUSP00000107717; ENSMUSG00000026414. [P50752-1]
    ENSMUST00000178854; ENSMUSP00000136265; ENSMUSG00000026414. [P50752-4]
    GeneIDi21956.
    KEGGimmu:21956.
    UCSCiuc007ctu.2. mouse. [P50752-4]
    uc007ctw.2. mouse. [P50752-3]
    uc007ctx.2. mouse. [P50752-2]
    uc011wso.1. mouse. [P50752-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47549 mRNA. Translation: AAA85345.1 .
    L47550 mRNA. Translation: AAA85346.1 .
    L47551 mRNA. Translation: AAA85347.1 .
    L47552 mRNA. Translation: AAA85348.1 .
    L47553 mRNA. Translation: AAA85349.1 .
    L47570 Genomic DNA. Translation: AAA85350.1 .
    L47599 mRNA. Translation: AAA85351.1 .
    L47600 mRNA. Translation: AAA85352.1 .
    CCDSi CCDS48376.1. [P50752-4 ]
    CCDS48377.1. [P50752-1 ]
    CCDS48378.1. [P50752-3 ]
    RefSeqi NP_001123648.1. NM_001130176.1. [P50752-1 ]
    NP_001123650.1. NM_001130178.2. [P50752-4 ]
    NP_001123651.4. NM_001130179.2.
    NP_001123652.1. NM_001130180.2. [P50752-3 ]
    NP_001123653.4. NM_001130181.2.
    NP_035749.1. NM_011619.3. [P50752-2 ]
    XP_006529456.1. XM_006529393.1. [P50752-1 ]
    XP_006529457.1. XM_006529394.1. [P50752-1 ]
    XP_006529458.1. XM_006529395.1. [P50752-3 ]
    UniGenei Mm.247470.

    3D structure databases

    ProteinModelPortali P50752.
    SMRi P50752. Positions 213-287.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P50752. 3 interactions.
    MINTi MINT-4138660.

    PTM databases

    PhosphoSitei P50752.

    Proteomic databases

    MaxQBi P50752.
    PaxDbi P50752.
    PRIDEi P50752.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027671 ; ENSMUSP00000027671 ; ENSMUSG00000026414 . [P50752-3 ]
    ENSMUST00000112087 ; ENSMUSP00000107717 ; ENSMUSG00000026414 . [P50752-1 ]
    ENSMUST00000178854 ; ENSMUSP00000136265 ; ENSMUSG00000026414 . [P50752-4 ]
    GeneIDi 21956.
    KEGGi mmu:21956.
    UCSCi uc007ctu.2. mouse. [P50752-4 ]
    uc007ctw.2. mouse. [P50752-3 ]
    uc007ctx.2. mouse. [P50752-2 ]
    uc011wso.1. mouse. [P50752-1 ]

    Organism-specific databases

    CTDi 7139.
    MGIi MGI:104597. Tnnt2.

    Phylogenomic databases

    eggNOGi NOG299534.
    GeneTreei ENSGT00390000013611.
    HOGENOMi HOG000231049.
    HOVERGENi HBG052790.
    KOi K12045.

    Miscellaneous databases

    ChiTaRSi TNNT2. mouse.
    NextBioi 301636.
    PMAP-CutDB P50752.
    PROi P50752.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P50752.
    Bgeei P50752.
    CleanExi MM_TNNT2.
    Genevestigatori P50752.

    Family and domain databases

    InterProi IPR027707. TNNT.
    IPR001978. Troponin.
    [Graphical view ]
    PANTHERi PTHR11521. PTHR11521. 1 hit.
    Pfami PF00992. Troponin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of mouse cardiac troponin T isoforms: characterization of a large sample of independent cDNA clones."
      Jin J.-P., Wang J., Zhang J.
      Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS A1; A2; A3B AND EMBRYONIC).
      Tissue: Heart.
    2. "Identification of a functionally critical protein kinase C phosphorylation residue of cardiac troponin T."
      Sumandea M.P., Pyle W.G., Kobayashi T., de Tombe P.P., Solaro R.J.
      J. Biol. Chem. 278:35135-35144(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-207; SER-211; THR-216 AND THR-297.

    Entry informationi

    Entry nameiTNNT2_MOUSE
    AccessioniPrimary (citable) accession number: P50752
    Secondary accession number(s): Q64360, Q64377
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3