P50752 (TNNT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Troponin T, cardiac muscle
Short name=TnTc

Alternative name(s):
Cardiac muscle troponin T
Short name=cTnT

Gene names

Name:

Tnnt2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Post-translational modification	Phosphorylation at Thr-216 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.
Sequence similarities	Belongs to the troponin T family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Muscle protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	atrial cardiac muscle tissue morphogenesis Inferred from mutant phenotype PubMed 10449439. Source: MGI muscle filament sliding Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of heart contraction Inferred from mutant phenotype PubMed 11158969PubMed 16326803. Source: MGI response to calcium ion Inferred from sequence or structural similarity. Source: UniProtKB sarcomere organization Inferred from mutant phenotype PubMed 10449439. Source: MGI ventricular cardiac muscle tissue morphogenesis Inferred from mutant phenotype PubMed 16326803. Source: MGI
Cellular_component	troponin complex Inferred from direct assay Ref.2. Source: MGI
Molecular_function	actin binding Inferred from sequence or structural similarity. Source: UniProtKB structural constituent of cytoskeleton Inferred from direct assay Ref.2. Source: MGI tropomyosin binding Inferred from sequence or structural similarity. Source: UniProtKB troponin C binding Inferred from sequence or structural similarity. Source: UniProtKB troponin I binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform Embryonic (identifier: P50752-1)

Also known as: EA;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A1 (identifier: P50752-4)

The sequence of this isoform differs from the canonical sequence as follows:
     18-26: EDWSEEEED → AVE

Isoform A2 (identifier: P50752-3)

The sequence of this isoform differs from the canonical sequence as follows:
     18-27: Missing.

Isoform A3B (identifier: P50752-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-27: Missing.
     204-206: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 301	300	Troponin T, cardiac muscle		PRO_0000186174
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	2	1	Phosphoserine; by CK2 By similarity
	Modified residue	207	1	Phosphothreonine; by PKC/PRKCA Ref.2
	Modified residue	211	1	Phosphoserine; by PKC/PRKCA Ref.2
	Modified residue	216	1	Phosphothreonine; by PKC/PRKCA and RAF1 Probable
	Modified residue	297	1	Phosphothreonine; by PKC/PRKCA Ref.2
Natural variations
	Alternative sequence	18 – 27	10	Missing in isoform A2 and isoform A3B.		VSP_006649
	Alternative sequence	18 – 26	9	EDWSEEEED → AVE in isoform A1.		VSP_007913
	Alternative sequence	204 – 206	3	Missing in isoform A3B.		VSP_006650

Sequences

	Sequence		Length	Mass (Da)
	Isoform Embryonic (EA) [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 60B2185A140AF2D5	FASTA	301	35,825
	10 20 30 40 50 60 MSDAEEVVEE YEEEQEEEDW SEEEEDEQEE AVEEEEAGGA EPEPEGEAET EEANVEEVGP 70 80 90 100 110 120 DEEAKDAEEG PVEDTKPKPS RLFMPNLVPP KIPDGERVDF DDIHRKRVEK DLNELQTLIE 130 140 150 160 170 180 AHFENRKKEE EELISLKDRI EKRRAERAEQ QRIRNEREKE RQNRLAEERA RREEEENRRK 190 200 210 220 230 240 AEDEARKKKA LSNMMHFGGY IQKQAQTERK SGKRQTEREK KKKILAERRK ALAIDHLNED 250 260 270 280 290 300 QLREKAKELW QSIHNLEAEK FDLQEKFKQQ KYEINVLRNR INDNQKVSKT RGKAKVTGRW K
	Isoform A1 [UniParc]. Checksum: 4B4C7ED7459289D5	FASTA	295	34,976
	10 20 30 40 50 60 MSDAEEVVEE YEEEQEEAVE EQEEAVEEEE AGGAEPEPEG EAETEEANVE EVGPDEEAKD 70 80 90 100 110 120 AEEGPVEDTK PKPSRLFMPN LVPPKIPDGE RVDFDDIHRK RVEKDLNELQ TLIEAHFENR 130 140 150 160 170 180 KKEEEELISL KDRIEKRRAE RAEQQRIRNE REKERQNRLA EERARREEEE NRRKAEDEAR 190 200 210 220 230 240 KKKALSNMMH FGGYIQKQAQ TERKSGKRQT EREKKKKILA ERRKALAIDH LNEDQLREKA 250 260 270 280 290 KELWQSIHNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK
	Isoform A2 [UniParc]. Checksum: 9BDA2958F567B11F	FASTA	291	34,547
	10 20 30 40 50 60 MSDAEEVVEE YEEEQEEQEE AVEEEEAGGA EPEPEGEAET EEANVEEVGP DEEAKDAEEG 70 80 90 100 110 120 PVEDTKPKPS RLFMPNLVPP KIPDGERVDF DDIHRKRVEK DLNELQTLIE AHFENRKKEE 130 140 150 160 170 180 EELISLKDRI EKRRAERAEQ QRIRNEREKE RQNRLAEERA RREEEENRRK AEDEARKKKA 190 200 210 220 230 240 LSNMMHFGGY IQKQAQTERK SGKRQTEREK KKKILAERRK ALAIDHLNED QLREKAKELW 250 260 270 280 290 QSIHNLEAEK FDLQEKFKQQ KYEINVLRNR INDNQKVSKT RGKAKVTGRW K
	Isoform A3B [UniParc]. Checksum: 002769E8DC4715B1	FASTA	288	34,220
	10 20 30 40 50 60 MSDAEEVVEE YEEEQEEQEE AVEEEEAGGA EPEPEGEAET EEANVEEVGP DEEAKDAEEG 70 80 90 100 110 120 PVEDTKPKPS RLFMPNLVPP KIPDGERVDF DDIHRKRVEK DLNELQTLIE AHFENRKKEE 130 140 150 160 170 180 EELISLKDRI EKRRAERAEQ QRIRNEREKE RQNRLAEERA RREEEENRRK AEDEARKKKA 190 200 210 220 230 240 LSNMMHFGGY IQKTERKSGK RQTEREKKKK ILAERRKALA IDHLNEDQLR EKAKELWQSI 250 260 270 280 HNLEAEKFDL QEKFKQQKYE INVLRNRIND NQKVSKTRGK AKVTGRWK

References

[1]	"Expression of mouse cardiac troponin T isoforms: characterization of a large sample of independent cDNA clones." Jin J.-P., Wang J., Zhang J. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS A1; A2; A3B AND EMBRYONIC). Tissue: Heart.
[2]	"Identification of a functionally critical protein kinase C phosphorylation residue of cardiac troponin T." Sumandea M.P., Pyle W.G., Kobayashi T., de Tombe P.P., Solaro R.J. J. Biol. Chem. 278:35135-35144(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT THR-207; SER-211; THR-216 AND THR-297.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L47549 mRNA. Translation: AAA85345.1. L47550 mRNA. Translation: AAA85346.1. L47551 mRNA. Translation: AAA85347.1. L47552 mRNA. Translation: AAA85348.1. L47553 mRNA. Translation: AAA85349.1. L47570 Genomic DNA. Translation: AAA85350.1. L47599 mRNA. Translation: AAA85351.1. L47600 mRNA. Translation: AAA85352.1.
IPI	IPI00336918. IPI00757310. IPI00828918. IPI00875142.
RefSeq	NP_001123648.1. NM_001130176.1. NP_001123650.1. NM_001130178.2. NP_001123651.4. NM_001130179.2. NP_001123652.1. NM_001130180.2. NP_001123653.4. NM_001130181.2. NP_035749.1. NM_011619.3.
UniGene	Mm.247470.
3D structure databases
ProteinModelPortal	P50752.
SMR	P50752. Positions 213-287.
ModBase	Search...
PTM databases
PhosphoSite	P50752.
Proteomic databases
PaxDb	P50752.
PRIDE	P50752.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027671; ENSMUSP00000027671; ENSMUSG00000026414. ENSMUST00000112087; ENSMUSP00000107717; ENSMUSG00000026414. ENSMUST00000178854; ENSMUSP00000136265; ENSMUSG00000026414.
GeneID	21956.
KEGG	mmu:21956.
UCSC	uc007ctu.2. mouse. uc007ctw.2. mouse. uc007ctx.2. mouse.
Organism-specific databases
CTD	7139.
MGI	MGI:104597. Tnnt2.
Phylogenomic databases
eggNOG	NOG299534.
GeneTree	ENSGT00390000013611.
HOGENOM	HOG000231049.
HOVERGEN	HBG052790.
KO	K12045.
OrthoDB	EOG4MCX1P.
Gene expression databases
ArrayExpress	P50752.
Bgee	P50752.
CleanEx	MM_TNNT2.
Genevestigator	P50752.
GermOnline	ENSMUSG00000026414. Mus musculus.
Family and domain databases
InterPro	IPR001978. Troponin. [Graphical view]
Pfam	PF00992. Troponin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TNNT2. mouse.
NextBio	301636.
PMAP-CutDB	P50752.
SOURCE	Search...

Entry information

Entry name

TNNT2_MOUSE

Accession

Primary (citable) accession number: P50752
Secondary accession number(s): Q64360, Q64377

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program