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P50752 (TNNT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Troponin T, cardiac muscle

Short name=TnTc
Alternative name(s):
Cardiac muscle troponin T
Short name=cTnT
Gene names
Name:Tnnt2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Post-translational modification

Phosphorylation at Thr-216 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

Sequence similarities

Belongs to the troponin T family.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   Molecular functionMuscle protein
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processatrial cardiac muscle tissue morphogenesis

Inferred from mutant phenotype PubMed 10449439. Source: MGI

heart development

Inferred from mutant phenotype PubMed 9637714. Source: MGI

muscle contraction

Inferred from direct assay Ref.2. Source: MGI

muscle filament sliding

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of ATPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of ATPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of heart contraction

Inferred from mutant phenotype PubMed 11158969PubMed 16326803. Source: MGI

regulation of muscle contraction

Inferred from electronic annotation. Source: InterPro

response to calcium ion

Inferred from sequence or structural similarity. Source: UniProtKB

sarcomere organization

Inferred from mutant phenotype PubMed 10449439. Source: MGI

ventricular cardiac muscle tissue morphogenesis

Inferred from mutant phenotype PubMed 16326803. Source: MGI

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 15830381. Source: MGI

myofibril

Inferred from direct assay PubMed 10449439PubMed 18781631. Source: MGI

sarcomere

Inferred from direct assay PubMed 12738802PubMed 16326803. Source: MGI

striated muscle thin filament

Inferred from sequence or structural similarity. Source: UniProtKB

troponin complex

Inferred from direct assay Ref.2. Source: MGI

   Molecular_functionactin binding

Inferred from sequence or structural similarity. Source: UniProtKB

structural constituent of cytoskeleton

Inferred from direct assay Ref.2. Source: MGI

tropomyosin binding

Inferred from sequence or structural similarity. Source: UniProtKB

troponin C binding

Inferred from sequence or structural similarity. Source: UniProtKB

troponin I binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform Embryonic (identifier: P50752-1)

Also known as: EA;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A1 (identifier: P50752-4)

The sequence of this isoform differs from the canonical sequence as follows:
     18-26: EDWSEEEED → AVE
Isoform A2 (identifier: P50752-3)

The sequence of this isoform differs from the canonical sequence as follows:
     18-27: Missing.
Isoform A3B (identifier: P50752-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-27: Missing.
     204-206: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 301300Troponin T, cardiac muscle
PRO_0000186174

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue21Phosphoserine; by CK2 By similarity
Modified residue2071Phosphothreonine; by PKC/PRKCA Ref.2
Modified residue2111Phosphoserine; by PKC/PRKCA Ref.2
Modified residue2161Phosphothreonine; by PKC/PRKCA and RAF1 Probable
Modified residue2971Phosphothreonine; by PKC/PRKCA Ref.2

Natural variations

Alternative sequence18 – 2710Missing in isoform A2 and isoform A3B.
VSP_006649
Alternative sequence18 – 269EDWSEEEED → AVE in isoform A1.
VSP_007913
Alternative sequence204 – 2063Missing in isoform A3B.
VSP_006650

Sequences

Sequence LengthMass (Da)Tools
Isoform Embryonic (EA) [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 60B2185A140AF2D5

FASTA30135,825
        10         20         30         40         50         60 
MSDAEEVVEE YEEEQEEEDW SEEEEDEQEE AVEEEEAGGA EPEPEGEAET EEANVEEVGP 

        70         80         90        100        110        120 
DEEAKDAEEG PVEDTKPKPS RLFMPNLVPP KIPDGERVDF DDIHRKRVEK DLNELQTLIE 

       130        140        150        160        170        180 
AHFENRKKEE EELISLKDRI EKRRAERAEQ QRIRNEREKE RQNRLAEERA RREEEENRRK 

       190        200        210        220        230        240 
AEDEARKKKA LSNMMHFGGY IQKQAQTERK SGKRQTEREK KKKILAERRK ALAIDHLNED 

       250        260        270        280        290        300 
QLREKAKELW QSIHNLEAEK FDLQEKFKQQ KYEINVLRNR INDNQKVSKT RGKAKVTGRW 


K 

« Hide

Isoform A1 [UniParc].

Checksum: 4B4C7ED7459289D5
Show »

FASTA29534,976
Isoform A2 [UniParc].

Checksum: 9BDA2958F567B11F
Show »

FASTA29134,547
Isoform A3B [UniParc].

Checksum: 002769E8DC4715B1
Show »

FASTA28834,220

References

[1]"Expression of mouse cardiac troponin T isoforms: characterization of a large sample of independent cDNA clones."
Jin J.-P., Wang J., Zhang J.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS A1; A2; A3B AND EMBRYONIC).
Tissue: Heart.
[2]"Identification of a functionally critical protein kinase C phosphorylation residue of cardiac troponin T."
Sumandea M.P., Pyle W.G., Kobayashi T., de Tombe P.P., Solaro R.J.
J. Biol. Chem. 278:35135-35144(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-207; SER-211; THR-216 AND THR-297.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L47549 mRNA. Translation: AAA85345.1.
L47550 mRNA. Translation: AAA85346.1.
L47551 mRNA. Translation: AAA85347.1.
L47552 mRNA. Translation: AAA85348.1.
L47553 mRNA. Translation: AAA85349.1.
L47570 Genomic DNA. Translation: AAA85350.1.
L47599 mRNA. Translation: AAA85351.1.
L47600 mRNA. Translation: AAA85352.1.
RefSeqNP_001123648.1. NM_001130176.1.
NP_001123650.1. NM_001130178.2.
NP_001123651.4. NM_001130179.2.
NP_001123652.1. NM_001130180.2.
NP_001123653.4. NM_001130181.2.
NP_035749.1. NM_011619.3.
XP_006529456.1. XM_006529393.1.
XP_006529457.1. XM_006529394.1.
XP_006529458.1. XM_006529395.1.
UniGeneMm.247470.

3D structure databases

ProteinModelPortalP50752.
SMRP50752. Positions 213-287.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP50752. 3 interactions.
MINTMINT-4138660.

PTM databases

PhosphoSiteP50752.

Proteomic databases

PaxDbP50752.
PRIDEP50752.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027671; ENSMUSP00000027671; ENSMUSG00000026414. [P50752-3]
ENSMUST00000112087; ENSMUSP00000107717; ENSMUSG00000026414. [P50752-1]
ENSMUST00000178854; ENSMUSP00000136265; ENSMUSG00000026414. [P50752-4]
GeneID21956.
KEGGmmu:21956.
UCSCuc007ctu.2. mouse. [P50752-4]
uc007ctw.2. mouse. [P50752-3]
uc007ctx.2. mouse. [P50752-2]
uc011wso.1. mouse. [P50752-1]

Organism-specific databases

CTD7139.
MGIMGI:104597. Tnnt2.

Phylogenomic databases

eggNOGNOG299534.
GeneTreeENSGT00390000013611.
HOGENOMHOG000231049.
HOVERGENHBG052790.
KOK12045.

Gene expression databases

ArrayExpressP50752.
BgeeP50752.
CleanExMM_TNNT2.
GenevestigatorP50752.

Family and domain databases

InterProIPR027707. TNNT.
IPR001978. Troponin.
[Graphical view]
PANTHERPTHR11521. PTHR11521. 1 hit.
PfamPF00992. Troponin. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTNNT2. mouse.
NextBio301636.
PMAP-CutDBP50752.
PROP50752.
SOURCESearch...

Entry information

Entry nameTNNT2_MOUSE
AccessionPrimary (citable) accession number: P50752
Secondary accession number(s): Q64360, Q64377
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot