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Protein

SH2B adapter protein 3

Gene

Sh2b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.

GO - Molecular functioni

  1. phosphate ion binding Source: RGD
  2. protein complex binding Source: RGD

GO - Biological processi

  1. cell differentiation Source: Ensembl
  2. embryonic hemopoiesis Source: Ensembl
  3. intracellular signal transduction Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_196232. Factors involved in megakaryocyte development and platelet production.
REACT_197451. Regulation of KIT signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
SH2B adapter protein 3
Alternative name(s):
Lymphocyte adapter protein
Lymphocyte-specific adapter protein Lnk
Signal transduction protein Lnk
Gene namesi
Name:Sh2b3
Synonyms:Lnk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 12

Organism-specific databases

RGDi68411. Sh2b3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368SH2B adapter protein 3PRO_0000084456Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei356 – 3561PhosphotyrosineSequence Analysis

Post-translational modificationi

Tyrosine-phosphorylated upon T-cell activation. The tyrosine phosphorylation site is multifunctional and may associate with the SH2 domains of phospholipase C-gamma-1, GRB-2, and PI3-K upon T-cell receptor activation.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP50745.

Expressioni

Tissue specificityi

Preferentially expressed by lymphocytes in lymph node and spleen.

Gene expression databases

ExpressionAtlasiP50745. baseline and differential.
GenevestigatoriP50745.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038125.

Structurei

3D structure databases

ProteinModelPortaliP50745.
SMRiP50745. Positions 114-217.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini119 – 21799SH2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SH2B adapter family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiNOG77816.
GeneTreeiENSGT00530000063355.
InParanoidiP50745.
KOiK12459.
OrthoDBiEOG70CR6F.
PhylomeDBiP50745.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Lnk4 (identifier: P50745-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGRTQGKHR PRVRTCPSLV PFDNPCWPPS HQPLPLQVGA PGHRVTSFLS
60 70 80 90 100
GRAWPSYIPK GKHGLPGPRY VPDPWSLGVL GHVAPLRVTT TIFLPGASPG
110 120 130 140 150
VMLDPACQKT DHFLSCYPWF HGPISRVRAA QLVQLQGPDA HGVFLVRQSE
160 170 180 190 200
SRRGEYVLTF NLQGRAKHLR LVLTERGQCR VQHLHFPSVV DMLRHFQRSP
210 220 230 240 250
IPLECGAACD VRLSGYVVVV SQAPGRKPKV VSRGGVTAST ARTKLPSWLR
260 270 280 290 300
LLHGVIHVIS PGSSNTVLFP FSLPHWDSEL GHPHLSSAGC PPGHGAEALR
310 320 330 340 350
GQVTPPEQIF HLVPSPEELA NSLRQLELES VSSARDSDYE MDSSSRSHLR
360
AIDNQYTPLS QLCREANL
Length:368
Mass (Da):40,432
Last modified:October 1, 1996 - v1
Checksum:i624CC2ABA0332C87
GO
Isoform Lnk1 (identifier: P50745-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-278: Missing.

Show »
Length:314
Mass (Da):34,602
Checksum:i613AF6E05E5DF652
GO
Isoform Lnk2 (identifier: P50745-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-95: MAGRTQGKHR...LRVTTTIFLP → MPDNLYTFVL...PRGSTDSLDQ
     226-262: Missing.

Show »
Length:309
Mass (Da):34,010
Checksum:i4E64228C4EC2C784
GO
Isoform Lnk3 (identifier: P50745-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     14-38: Missing.
     226-262: Missing.

Show »
Length:306
Mass (Da):33,663
Checksum:iCEEBD99D1208689A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9595MAGRT…TIFLP → MPDNLYTFVLKVQGQTDIIF EVGDEQQLNSWLAELRASTG LGLEHLDTELPLSLVAEPGP AISPRGSTDSLDQ in isoform Lnk2. 1 PublicationVSP_004308Add
BLAST
Alternative sequencei14 – 3825Missing in isoform Lnk3. 1 PublicationVSP_004309Add
BLAST
Alternative sequencei225 – 27854Missing in isoform Lnk1. 1 PublicationVSP_004310Add
BLAST
Alternative sequencei226 – 26237Missing in isoform Lnk2 and isoform Lnk3. 1 PublicationVSP_004311Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24655 mRNA. Translation: AAC52351.1.
U24654 mRNA. Translation: AAC52350.1.
U24653 mRNA. Translation: AAC52349.1.
U24652 mRNA. Translation: AAC52348.1.
RefSeqiNP_113809.1. NM_031621.1. [P50745-1]
UniGeneiRn.11228.

Genome annotation databases

EnsembliENSRNOT00000030583; ENSRNOP00000038125; ENSRNOG00000028198. [P50745-3]
ENSRNOT00000073611; ENSRNOP00000066850; ENSRNOG00000028198. [P50745-1]
ENSRNOT00000075776; ENSRNOP00000065142; ENSRNOG00000028198. [P50745-4]
GeneIDi58838.
KEGGirno:58838.
UCSCiRGD:68411. rat. [P50745-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24655 mRNA. Translation: AAC52351.1.
U24654 mRNA. Translation: AAC52350.1.
U24653 mRNA. Translation: AAC52349.1.
U24652 mRNA. Translation: AAC52348.1.
RefSeqiNP_113809.1. NM_031621.1. [P50745-1]
UniGeneiRn.11228.

3D structure databases

ProteinModelPortaliP50745.
SMRiP50745. Positions 114-217.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038125.

Proteomic databases

PRIDEiP50745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000030583; ENSRNOP00000038125; ENSRNOG00000028198. [P50745-3]
ENSRNOT00000073611; ENSRNOP00000066850; ENSRNOG00000028198. [P50745-1]
ENSRNOT00000075776; ENSRNOP00000065142; ENSRNOG00000028198. [P50745-4]
GeneIDi58838.
KEGGirno:58838.
UCSCiRGD:68411. rat. [P50745-1]

Organism-specific databases

CTDi10019.
RGDi68411. Sh2b3.

Phylogenomic databases

eggNOGiNOG77816.
GeneTreeiENSGT00530000063355.
InParanoidiP50745.
KOiK12459.
OrthoDBiEOG70CR6F.
PhylomeDBiP50745.

Enzyme and pathway databases

ReactomeiREACT_196232. Factors involved in megakaryocyte development and platelet production.
REACT_197451. Regulation of KIT signaling.

Miscellaneous databases

NextBioi611402.
PROiP50745.

Gene expression databases

ExpressionAtlasiP50745. baseline and differential.
GenevestigatoriP50745.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of Lnk, a signal transduction protein that links T-cell receptor activation signal to phospholipase C gamma 1, Grb2, and phosphatidylinositol 3-kinase."
    Huang X., Li Y., Tanaka K., Moore K.G., Hayashi J.
    Proc. Natl. Acad. Sci. U.S.A. 92:11618-11622(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LNK1; LNK2; LNK3 AND LNK4).
    Strain: Fischer.
    Tissue: Lymph node.

Entry informationi

Entry nameiSH2B3_RAT
AccessioniPrimary (citable) accession number: P50745
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.