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P50743

- DER_BACSU

UniProt

P50743 - DER_BACSU

Protein

GTPase Der

Gene

der

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    GTPase that plays an essential role in the late steps of ribosome biogenesis.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTP 1Sequence Analysis
    Nucleotide bindingi57 – 615GTP 1Sequence Analysis
    Nucleotide bindingi119 – 1224GTP 1Sequence Analysis
    Nucleotide bindingi182 – 1898GTP 2Sequence Analysis
    Nucleotide bindingi229 – 2335GTP 2Sequence Analysis
    Nucleotide bindingi294 – 2974GTP 2Sequence Analysis

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. ribosome biogenesis Source: UniProtKB-KW

    Keywords - Biological processi

    Ribosome biogenesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU22840-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTPase Der
    Alternative name(s):
    GTP-binding protein EngA
    Gene namesi
    Name:der
    Synonyms:engA, yphC
    Ordered Locus Names:BSU22840
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU22840. [Micado]

    Pathology & Biotechi

    Disruption phenotypei

    Essential for growth, it cannot be disrupted. Depletion experiments show that cells become longer and abnormally curved, with nucleoid condensation. Cells have many fewer 70S ribosomes; the large ribosomal subunit is 45S and is missing proteins L16, L27, L36 and interacts with 2 large non-ribosomal proteins.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 436436GTPase DerPRO_0000178964Add
    BLAST

    Proteomic databases

    PaxDbiP50743.

    Interactioni

    Subunit structurei

    Cofractionates with the 70S ribosome; association is stabilized by the non-hydrolyzable GTP analog GMPPNP, and inhibited by GTP or GDP.

    Protein-protein interaction databases

    STRINGi224308.BSU22840.

    Structurei

    Secondary structure

    1
    436
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 95
    Beta strandi11 – 155
    Helixi16 – 238
    Beta strandi24 – 285
    Beta strandi42 – 454
    Beta strandi54 – 563
    Helixi69 – 8214
    Beta strandi84 – 9411
    Helixi98 – 10710
    Beta strandi114 – 1196
    Helixi134 – 1363
    Beta strandi137 – 1437
    Turni146 – 1483
    Helixi152 – 1609
    Helixi161 – 1655
    Beta strandi176 – 1816
    Helixi188 – 1969
    Beta strandi201 – 2033
    Beta strandi215 – 2206
    Beta strandi223 – 2275
    Helixi230 – 2323
    Turni233 – 2353
    Turni236 – 2383
    Helixi245 – 25713
    Beta strandi259 – 2668
    Turni267 – 2693
    Helixi273 – 28412
    Beta strandi288 – 2947
    Helixi296 – 2983
    Helixi305 – 31612
    Helixi318 – 3203
    Beta strandi325 – 3273
    Turni330 – 3323
    Helixi336 – 3383
    Helixi339 – 35012
    Helixi356 – 36914
    Beta strandi374 – 3774
    Beta strandi382 – 3898
    Turni390 – 3934
    Beta strandi394 – 4007
    Helixi402 – 4043
    Helixi407 – 42115
    Beta strandi429 – 4335

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HJGX-ray2.50A1-436[»]
    4DCSX-ray2.25A1-436[»]
    4DCTX-ray2.30A1-436[»]
    4DCUX-ray2.00A1-436[»]
    4DCVX-ray2.60A1-436[»]
    4KYUX-ray1.70A/B1-163[»]
    ProteinModelPortaliP50743.
    SMRiP50743. Positions 3-436.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP50743.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 167164EngA-type G 1Add
    BLAST
    Domaini176 – 351176EngA-type G 2Add
    BLAST
    Domaini352 – 43685KH-likeAdd
    BLAST

    Sequence similaritiesi

    Contains 1 KH-like domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1160.
    HOGENOMiHOG000242861.
    KOiK03977.
    OMAiSQGRGIN.
    OrthoDBiEOG6DC6K1.
    PhylomeDBiP50743.

    Family and domain databases

    Gene3Di3.30.300.20. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPiMF_00195. GTPase_Der.
    InterProiIPR016484. GTP-bd_EngA.
    IPR006073. GTP_binding_domain.
    IPR015946. KH_dom-like_a/b.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    [Graphical view]
    PANTHERiPTHR11649:SF5. PTHR11649:SF5. 1 hit.
    PfamiPF01926. MMR_HSR1. 2 hits.
    [Graphical view]
    PIRSFiPIRSF006485. GTP-binding_EngA. 1 hit.
    SUPFAMiSSF52540. SSF52540. 2 hits.
    TIGRFAMsiTIGR03594. GTPase_EngA. 1 hit.
    TIGR00231. small_GTP. 2 hits.
    PROSITEiPS51712. G_ENGA. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50743-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKPVVAIVG RPNVGKSTIF NRIAGERISI VEDTPGVTRD RIYSSAEWLN    50
    YDFNLIDTGG IDIGDEPFLA QIRQQAEIAM DEADVIIFMV NGREGVTAAD 100
    EEVAKILYRT KKPVVLAVNK LDNTEMRANI YDFYSLGFGE PYPISGTHGL 150
    GLGDLLDAVA EHFKNIPETK YNEEVIQFCL IGRPNVGKSS LVNAMLGEER 200
    VIVSNVAGTT RDAVDTSFTY NQQEFVIVDT AGMRKKGKVY ETTEKYSVLR 250
    ALKAIDRSEV VAVVLDGEEG IIEQDKRIAG YAHEAGKAVV IVVNKWDAVD 300
    KDESTMKEFE ENIRDHFQFL DYAPILFMSA LTKKRIHTLM PAIIKASENH 350
    SLRVQTNVLN DVIMDAVAMN PTPTHNGSRL KIYYATQVSV KPPSFVVFVN 400
    DPELMHFSYE RFLENRIRDA FGFEGTPIKI FARARK 436
    Length:436
    Mass (Da):48,770
    Last modified:October 1, 1996 - v1
    Checksum:iA5CC7028FFB5A442
    GO

    Sequence cautioni

    The sequence U32164 differs from that shown. Reason: Frameshift at position 208.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti186 – 1872VG → CR(PubMed:9384377)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47648 Genomic DNA. Translation: AAC83966.1.
    AL009126 Genomic DNA. Translation: CAB14200.1.
    U32164 Genomic DNA. No translation available.
    PIRiA69936.
    RefSeqiNP_390165.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB14200; CAB14200; BSU22840.
    GeneIDi938988.
    KEGGibsu:BSU22840.
    PATRICi18976379. VBIBacSub10457_2380.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L47648 Genomic DNA. Translation: AAC83966.1 .
    AL009126 Genomic DNA. Translation: CAB14200.1 .
    U32164 Genomic DNA. No translation available.
    PIRi A69936.
    RefSeqi NP_390165.1. NC_000964.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HJG X-ray 2.50 A 1-436 [» ]
    4DCS X-ray 2.25 A 1-436 [» ]
    4DCT X-ray 2.30 A 1-436 [» ]
    4DCU X-ray 2.00 A 1-436 [» ]
    4DCV X-ray 2.60 A 1-436 [» ]
    4KYU X-ray 1.70 A/B 1-163 [» ]
    ProteinModelPortali P50743.
    SMRi P50743. Positions 3-436.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU22840.

    Proteomic databases

    PaxDbi P50743.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14200 ; CAB14200 ; BSU22840 .
    GeneIDi 938988.
    KEGGi bsu:BSU22840.
    PATRICi 18976379. VBIBacSub10457_2380.

    Organism-specific databases

    GenoListi BSU22840. [Micado ]

    Phylogenomic databases

    eggNOGi COG1160.
    HOGENOMi HOG000242861.
    KOi K03977.
    OMAi SQGRGIN.
    OrthoDBi EOG6DC6K1.
    PhylomeDBi P50743.

    Enzyme and pathway databases

    BioCyci BSUB:BSU22840-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P50743.

    Family and domain databases

    Gene3Di 3.30.300.20. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPi MF_00195. GTPase_Der.
    InterProi IPR016484. GTP-bd_EngA.
    IPR006073. GTP_binding_domain.
    IPR015946. KH_dom-like_a/b.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11649:SF5. PTHR11649:SF5. 1 hit.
    Pfami PF01926. MMR_HSR1. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF006485. GTP-binding_EngA. 1 hit.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    TIGRFAMsi TIGR03594. GTPase_EngA. 1 hit.
    TIGR00231. small_GTP. 2 hits.
    PROSITEi PS51712. G_ENGA. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome."
      Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P.
      Microbiology 142:2005-2016(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Synthesis of sn-glycerol 3-phosphate, a key precursor of membrane lipids, in Bacillus subtilis."
      Morbidoni H.R., de Mendoza D., Cronan J.E. Jr.
      J. Bacteriol. 177:5899-5905(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 185-436.
      Strain: 168.
    4. "Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis."
      Morimoto T., Loh P.C., Hirai T., Asai K., Kobayashi K., Moriya S., Ogasawara N.
      Microbiology 148:3539-3552(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GTP- AND GDP-BINDING, PROTEIN LEVELS, DISRUPTION PHENOTYPE.
      Strain: CRK6000.
    5. "Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis."
      Schaefer L., Uicker W.C., Wicker-Planquart C., Foucher A.-E., Jault J.-M., Britton R.A.
      J. Bacteriol. 188:8252-8258(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THE 50S RIBOSOMAL SUBUNIT, FUNCTION IN 50S RIBOSOMAL SUBUNIT BIOGENESIS.
      Strain: RB247.
    6. "The essential GTPase YphC displays a major domain rearrangement associated with nucleotide binding."
      Muench S.P., Xu L., Sedelnikova S.E., Rice D.W.
      Proc. Natl. Acad. Sci. U.S.A. 103:12359-12364(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) BOUND TO GDP IN THE PROBABLE OFF STATE.

    Entry informationi

    Entry nameiDER_BACSU
    AccessioniPrimary (citable) accession number: P50743
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Estimated to be present at 7000 copies per cell.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3