P50743 (DER_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase Der

Alternative name(s):
GTP-binding protein EngA

Gene names

Name:	der
Synonyms:	engA, yphC
Ordered Locus Names:	BSU22840

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase that plays an essential role in the late steps of ribosome biogenesis. Ref.5
Subunit structure	Cofractionates with the 70S ribosome; association is stabilized by the non-hydrolyzable GTP analog GMPPNP, and inhibited by GTP or GDP. Ref.5
Disruption phenotype	Essential for growth, it cannot be disrupted. Depletion experiments show that cells become longer and abnormally curved, with nucleoid condensation. Cells have many fewer 70S ribosomes; the large ribosomal subunit is 45S and is missing proteins L16, L27, L36 and interacts with 2 large non-ribosomal proteins. Ref.4
Miscellaneous	Estimated to be present at 7000 copies per cell. HAMAP-Rule MF_00195
Sequence similarities	Belongs to the Era/MnmE GTP-binding protein family. Der subfamily. Contains 2 G (guanine nucleotide-binding) domains. Contains 1 KH-like domain.
Sequence caution	The sequence U32164 differs from that shown. Reason: Frameshift at position 208.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Domain	Repeat
Ligand	GTP-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ribosome biogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 436

436

GTPase Der HAMAP-Rule MF_00195

PRO_0000178964

Regions

Domain

15 – 120

106

G 1

Domain

187 – 295

109

G 2

Domain

351 – 436

86

KH-like

Nucleotide binding

10 – 17

8

GTP 1 Potential

Nucleotide binding

57 – 61

5

GTP 1 Potential

Nucleotide binding

119 – 122

4

GTP 1 Potential

Nucleotide binding

182 – 189

8

GTP 2 Potential

Nucleotide binding

229 – 233

5

GTP 2 Potential

Nucleotide binding

294 – 297

4

GTP 2 Potential

Experimental info

Sequence conflict

186 – 187

2

VG → CR Ref.2

Secondary structure

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436

Helix Strand Turn

Details...

Beta strand

5 – 9

5

Beta strand

11 – 15

5

Helix

16 – 23

8

Beta strand

24 – 28

5

Beta strand

42 – 45

4

Beta strand

54 – 56

3

Helix

69 – 82

14

Beta strand

84 – 94

11

Helix

98 – 107

10

Beta strand

114 – 119

6

Helix

134 – 136

3

Beta strand

137 – 143

7

Turn

146 – 148

3

Helix

152 – 160

9

Helix

161 – 165

5

Beta strand

176 – 181

6

Helix

188 – 196

9

Beta strand

201 – 203

3

Beta strand

215 – 220

6

Beta strand

223 – 227

5

Helix

230 – 232

3

Turn

233 – 235

3

Turn

236 – 238

3

Helix

245 – 257

13

Beta strand

259 – 266

8

Turn

267 – 269

3

Helix

273 – 284

12

Beta strand

288 – 294

7

Helix

296 – 298

3

Helix

305 – 316

12

Helix

318 – 320

3

Beta strand

325 – 327

3

Turn

330 – 332

3

Helix

336 – 338

3

Helix

339 – 350

12

Helix

356 – 369

14

Beta strand

374 – 377

4

Beta strand

382 – 389

8

Turn

390 – 393

4

Beta strand

394 – 400

7

Helix

402 – 404

3

Helix

407 – 421

15

Beta strand

429 – 433

5

Sequences

	Sequence		Length	Mass (Da)
	P50743 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A5CC7028FFB5A442	FASTA	436	48,770
	10 20 30 40 50 60 MGKPVVAIVG RPNVGKSTIF NRIAGERISI VEDTPGVTRD RIYSSAEWLN YDFNLIDTGG 70 80 90 100 110 120 IDIGDEPFLA QIRQQAEIAM DEADVIIFMV NGREGVTAAD EEVAKILYRT KKPVVLAVNK 130 140 150 160 170 180 LDNTEMRANI YDFYSLGFGE PYPISGTHGL GLGDLLDAVA EHFKNIPETK YNEEVIQFCL 190 200 210 220 230 240 IGRPNVGKSS LVNAMLGEER VIVSNVAGTT RDAVDTSFTY NQQEFVIVDT AGMRKKGKVY 250 260 270 280 290 300 ETTEKYSVLR ALKAIDRSEV VAVVLDGEEG IIEQDKRIAG YAHEAGKAVV IVVNKWDAVD 310 320 330 340 350 360 KDESTMKEFE ENIRDHFQFL DYAPILFMSA LTKKRIHTLM PAIIKASENH SLRVQTNVLN 370 380 390 400 410 420 DVIMDAVAMN PTPTHNGSRL KIYYATQVSV KPPSFVVFVN DPELMHFSYE RFLENRIRDA 430 FGFEGTPIKI FARARK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome." Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P. Microbiology 142:2005-2016(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Synthesis of sn-glycerol 3-phosphate, a key precursor of membrane lipids, in Bacillus subtilis." Morbidoni H.R., de Mendoza D., Cronan J.E. Jr. J. Bacteriol. 177:5899-5905(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 185-436. Strain: 168.
[4]	"Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis." Morimoto T., Loh P.C., Hirai T., Asai K., Kobayashi K., Moriya S., Ogasawara N. Microbiology 148:3539-3552(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GTP- AND GDP-BINDING, PROTEIN LEVELS, DISRUPTION PHENOTYPE. Strain: CRK6000.
[5]	"Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis." Schaefer L., Uicker W.C., Wicker-Planquart C., Foucher A.-E., Jault J.-M., Britton R.A. J. Bacteriol. 188:8252-8258(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH THE 50S RIBOSOMAL SUBUNIT, FUNCTION IN 50S RIBOSOMAL SUBUNIT BIOGENESIS. Strain: RB247.
[6]	"The essential GTPase YphC displays a major domain rearrangement associated with nucleotide binding." Muench S.P., Xu L., Sedelnikova S.E., Rice D.W. Proc. Natl. Acad. Sci. U.S.A. 103:12359-12364(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) BOUND TO GDP IN THE PROBABLE OFF STATE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L47648 Genomic DNA. Translation: AAC83966.1.
AL009126 Genomic DNA. Translation: CAB14200.1.
U32164 Genomic DNA. No translation available.

PIR

A69936.

RefSeq

NP_390165.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HJG	X-ray	2.50	A	1-436	[»]
4DCS	X-ray	2.25	A	1-436	[»]
4DCT	X-ray	2.30	A	1-436	[»]
4DCU	X-ray	2.00	A	1-436	[»]
4DCV	X-ray	2.60	A	1-436	[»]

ProteinModelPortal

P50743.

SMR

P50743. Positions 3-436.

ModBase

Search...

Protein-protein interaction databases

STRING

224308.BSU22840.

Proteomic databases

PaxDb

P50743.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB14200; CAB14200; BSU22840.

GeneID

938988.

KEGG

bsu:BSU22840.

PATRIC

18976379. VBIBacSub10457_2380.

Organism-specific databases

GenoList

BSU22840. [Micado]

Phylogenomic databases

eggNOG

COG1160.

HOGENOM

HOG000242861.

KO

K03977.

OMA

YLENTYR.

ProtClustDB

PRK00093.

Enzyme and pathway databases

BioCyc

BSUB:BSU22840-MONOMER.

Family and domain databases

Gene3D

3.30.300.20. 1 hit.

HAMAP

MF_00195. GTPase_Der.

InterPro

IPR016484. GTP-bd_EngA.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR005225. Small_GTP-bd_dom.
[Graphical view]

PANTHER

PTHR11649:SF5. PTHR11649:SF5. 1 hit.

Pfam

PF01926. MMR_HSR1. 2 hits.
[Graphical view]

PIRSF

PIRSF006485. GTP-binding_EngA. 1 hit.

TIGRFAMs

TIGR03594. GTPase_EngA. 1 hit.
TIGR00231. small_GTP. 2 hits.

ProtoNet

Search...

Other

EvolutionaryTrace

P50743.

Entry information

Entry name

DER_BACSU

Accession

Primary (citable) accession number: P50743

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program