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P50743

- DER_BACSU

UniProt

P50743 - DER_BACSU

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Protein

GTPase Der

Gene
der, engA, yphC, BSU22840
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

GTPase that plays an essential role in the late steps of ribosome biogenesis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTP 1 Reviewed prediction
Nucleotide bindingi57 – 615GTP 1 Reviewed prediction
Nucleotide bindingi119 – 1224GTP 1 Reviewed prediction
Nucleotide bindingi182 – 1898GTP 2 Reviewed prediction
Nucleotide bindingi229 – 2335GTP 2 Reviewed prediction
Nucleotide bindingi294 – 2974GTP 2 Reviewed prediction

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. ribosome biogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU22840-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase Der
Alternative name(s):
GTP-binding protein EngA
Gene namesi
Name:der
Synonyms:engA, yphC
Ordered Locus Names:BSU22840
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU22840. [Micado]

Pathology & Biotechi

Disruption phenotypei

Essential for growth, it cannot be disrupted. Depletion experiments show that cells become longer and abnormally curved, with nucleoid condensation. Cells have many fewer 70S ribosomes; the large ribosomal subunit is 45S and is missing proteins L16, L27, L36 and interacts with 2 large non-ribosomal proteins.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436GTPase DerUniRule annotationPRO_0000178964Add
BLAST

Proteomic databases

PaxDbiP50743.

Interactioni

Subunit structurei

Cofractionates with the 70S ribosome; association is stabilized by the non-hydrolyzable GTP analog GMPPNP, and inhibited by GTP or GDP.1 Publication

Protein-protein interaction databases

STRINGi224308.BSU22840.

Structurei

Secondary structure

1
436
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Beta strandi11 – 155
Helixi16 – 238
Beta strandi24 – 285
Beta strandi42 – 454
Beta strandi54 – 563
Helixi69 – 8214
Beta strandi84 – 9411
Helixi98 – 10710
Beta strandi114 – 1196
Helixi134 – 1363
Beta strandi137 – 1437
Turni146 – 1483
Helixi152 – 1609
Helixi161 – 1655
Beta strandi176 – 1816
Helixi188 – 1969
Beta strandi201 – 2033
Beta strandi215 – 2206
Beta strandi223 – 2275
Helixi230 – 2323
Turni233 – 2353
Turni236 – 2383
Helixi245 – 25713
Beta strandi259 – 2668
Turni267 – 2693
Helixi273 – 28412
Beta strandi288 – 2947
Helixi296 – 2983
Helixi305 – 31612
Helixi318 – 3203
Beta strandi325 – 3273
Turni330 – 3323
Helixi336 – 3383
Helixi339 – 35012
Helixi356 – 36914
Beta strandi374 – 3774
Beta strandi382 – 3898
Turni390 – 3934
Beta strandi394 – 4007
Helixi402 – 4043
Helixi407 – 42115
Beta strandi429 – 4335

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HJGX-ray2.50A1-436[»]
4DCSX-ray2.25A1-436[»]
4DCTX-ray2.30A1-436[»]
4DCUX-ray2.00A1-436[»]
4DCVX-ray2.60A1-436[»]
4KYUX-ray1.70A/B1-163[»]
ProteinModelPortaliP50743.
SMRiP50743. Positions 3-436.

Miscellaneous databases

EvolutionaryTraceiP50743.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 167164EngA-type G 1Add
BLAST
Domaini176 – 351176EngA-type G 2Add
BLAST
Domaini352 – 43685KH-likeAdd
BLAST

Sequence similaritiesi

Contains 1 KH-like domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1160.
HOGENOMiHOG000242861.
KOiK03977.
OMAiSQGRGIN.
OrthoDBiEOG6DC6K1.
PhylomeDBiP50743.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00195. GTPase_Der.
InterProiIPR016484. GTP-bd_EngA.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
PANTHERiPTHR11649:SF5. PTHR11649:SF5. 1 hit.
PfamiPF01926. MMR_HSR1. 2 hits.
[Graphical view]
PIRSFiPIRSF006485. GTP-binding_EngA. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR03594. GTPase_EngA. 1 hit.
TIGR00231. small_GTP. 2 hits.
PROSITEiPS51712. G_ENGA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50743-1 [UniParc]FASTAAdd to Basket

« Hide

MGKPVVAIVG RPNVGKSTIF NRIAGERISI VEDTPGVTRD RIYSSAEWLN    50
YDFNLIDTGG IDIGDEPFLA QIRQQAEIAM DEADVIIFMV NGREGVTAAD 100
EEVAKILYRT KKPVVLAVNK LDNTEMRANI YDFYSLGFGE PYPISGTHGL 150
GLGDLLDAVA EHFKNIPETK YNEEVIQFCL IGRPNVGKSS LVNAMLGEER 200
VIVSNVAGTT RDAVDTSFTY NQQEFVIVDT AGMRKKGKVY ETTEKYSVLR 250
ALKAIDRSEV VAVVLDGEEG IIEQDKRIAG YAHEAGKAVV IVVNKWDAVD 300
KDESTMKEFE ENIRDHFQFL DYAPILFMSA LTKKRIHTLM PAIIKASENH 350
SLRVQTNVLN DVIMDAVAMN PTPTHNGSRL KIYYATQVSV KPPSFVVFVN 400
DPELMHFSYE RFLENRIRDA FGFEGTPIKI FARARK 436
Length:436
Mass (Da):48,770
Last modified:October 1, 1996 - v1
Checksum:iA5CC7028FFB5A442
GO

Sequence cautioni

The sequence U32164 differs from that shown. Reason: Frameshift at position 208.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1872VG → CR1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L47648 Genomic DNA. Translation: AAC83966.1.
AL009126 Genomic DNA. Translation: CAB14200.1.
U32164 Genomic DNA. No translation available.
PIRiA69936.
RefSeqiNP_390165.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14200; CAB14200; BSU22840.
GeneIDi938988.
KEGGibsu:BSU22840.
PATRICi18976379. VBIBacSub10457_2380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L47648 Genomic DNA. Translation: AAC83966.1 .
AL009126 Genomic DNA. Translation: CAB14200.1 .
U32164 Genomic DNA. No translation available.
PIRi A69936.
RefSeqi NP_390165.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HJG X-ray 2.50 A 1-436 [» ]
4DCS X-ray 2.25 A 1-436 [» ]
4DCT X-ray 2.30 A 1-436 [» ]
4DCU X-ray 2.00 A 1-436 [» ]
4DCV X-ray 2.60 A 1-436 [» ]
4KYU X-ray 1.70 A/B 1-163 [» ]
ProteinModelPortali P50743.
SMRi P50743. Positions 3-436.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU22840.

Proteomic databases

PaxDbi P50743.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14200 ; CAB14200 ; BSU22840 .
GeneIDi 938988.
KEGGi bsu:BSU22840.
PATRICi 18976379. VBIBacSub10457_2380.

Organism-specific databases

GenoListi BSU22840. [Micado ]

Phylogenomic databases

eggNOGi COG1160.
HOGENOMi HOG000242861.
KOi K03977.
OMAi SQGRGIN.
OrthoDBi EOG6DC6K1.
PhylomeDBi P50743.

Enzyme and pathway databases

BioCyci BSUB:BSU22840-MONOMER.

Miscellaneous databases

EvolutionaryTracei P50743.

Family and domain databases

Gene3Di 3.30.300.20. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00195. GTPase_Der.
InterProi IPR016484. GTP-bd_EngA.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view ]
PANTHERi PTHR11649:SF5. PTHR11649:SF5. 1 hit.
Pfami PF01926. MMR_HSR1. 2 hits.
[Graphical view ]
PIRSFi PIRSF006485. GTP-binding_EngA. 1 hit.
SUPFAMi SSF52540. SSF52540. 2 hits.
TIGRFAMsi TIGR03594. GTPase_EngA. 1 hit.
TIGR00231. small_GTP. 2 hits.
PROSITEi PS51712. G_ENGA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome."
    Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P.
    Microbiology 142:2005-2016(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Synthesis of sn-glycerol 3-phosphate, a key precursor of membrane lipids, in Bacillus subtilis."
    Morbidoni H.R., de Mendoza D., Cronan J.E. Jr.
    J. Bacteriol. 177:5899-5905(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 185-436.
    Strain: 168.
  4. "Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis."
    Morimoto T., Loh P.C., Hirai T., Asai K., Kobayashi K., Moriya S., Ogasawara N.
    Microbiology 148:3539-3552(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GTP- AND GDP-BINDING, PROTEIN LEVELS, DISRUPTION PHENOTYPE.
    Strain: CRK6000.
  5. "Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis."
    Schaefer L., Uicker W.C., Wicker-Planquart C., Foucher A.-E., Jault J.-M., Britton R.A.
    J. Bacteriol. 188:8252-8258(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THE 50S RIBOSOMAL SUBUNIT, FUNCTION IN 50S RIBOSOMAL SUBUNIT BIOGENESIS.
    Strain: RB247.
  6. "The essential GTPase YphC displays a major domain rearrangement associated with nucleotide binding."
    Muench S.P., Xu L., Sedelnikova S.E., Rice D.W.
    Proc. Natl. Acad. Sci. U.S.A. 103:12359-12364(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) BOUND TO GDP IN THE PROBABLE OFF STATE.

Entry informationi

Entry nameiDER_BACSU
AccessioniPrimary (citable) accession number: P50743
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Estimated to be present at 7000 copies per cell.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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