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Reviewed, UniProtKB/Swiss-Prot P50735 (GUDB_BACSU)

Last modified November 25, 2008. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NAD-specific glutamate dehydrogenase
      Short name=NAD-GDH
    EC=1.4.1.2
Gene names
Name: gudB
Synonyms: ypcA
Ordered Locus Names: BSU22960
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The wild-type version has little or no activity.

Catalytic activity

L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

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Ontologies

Keywords

   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processamino acid metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionbinding

Inferred from electronic annotation. Source: InterPro

glutamate dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426NAD-specific glutamate dehydrogenase
PRO_0000182736

Sites

Active site1181 By similarity

Natural variations

Natural variant96 – 983Missing in spontaneous mutant gudB1; restores activity.

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Sequences

Sequence LengthMass (Da)Tools
P50735-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 5624F2DE93333E86

FASTA42647,212
        10         20         30         40         50         60 
MAADRNTGHT EEDKLDVLKS TQTVIHKALE KLGYPEEVYE LLKEPMRLLT VKIPVRMDDG 

        70         80         90        100        110        120 
SVKIFTGYRA HNDSVGPTKG GIRFHPNVTE KEVKAVKALS IWMSLKCGII DLPYGGGKGG 

       130        140        150        160        170        180 
IVCDPRDMSF RELERLSRGY VRAISQIVGP TKDVPAPDVF TNSQIMAWMM DEYSRIDEFN 

       190        200        210        220        230        240 
SPGFITGKPL VLGGSHGRES ATAKGVTICI KEAAKKRGID IKGARVVVQG FGNAGSYLAK 

       250        260        270        280        290        300 
FMHDAGAKVV GISDAYGGLY DPEGLDIDYL LDRRDSFGTV TKLFNDTITN QELLELDCDI 

       310        320        330        340        350        360 
LVPAAIENQI TEENAHNIRA KIVVEAANGP TTLEGTKILS DRDILLVPDV LASAGGVTVS 

       370        380        390        400        410        420 
YFEWVQNNQG FYWSEEEVEE KLEKMMVKSF NNIYEMANNR RIDMRLAAYM VGVRKMAEAS 


RFRGWI

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References

« Hide 'large scale' references
[1]"Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome."
Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P.
Microbiology 142:2005-2016(1996) [PubMed: 8760912] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Role and regulation of Bacillus subtilis glutamate dehydrogenase genes."
Belitsky B.R., Sonenshein A.L.
J. Bacteriol. 180:6298-6305(1998) [PubMed: 9829940] [Abstract]
Cited for: CHARACTERIZATION.

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Cross-references

Sequence databases

L47648 Genomic DNA. Translation: AAC83953.1.
Z99115 Genomic DNA. Translation: CAB14212.1.
PIRG69933.
RefSeqNP_390177.1.

3D structure databases

HSSPHSSP built from PDB template 1GTM based on UniProtKB P80319.
ModBaseSearch...

Genome annotation databases

GeneID938975.
GenomeReviewsGene locus BSU22960 in contig AL009126_GR.
KEGGbsu:BSU22960.
NMPDRfig|224308.1.peg.2300.

Organism-specific databases

SubtiListBG11435. gudB. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP50735.

Enzyme and pathway databases

BioCycBSUB224308:BSU2295-MON.

Family and domain databases

InterProIPR006095. Glu/Leu/Phe/Val_DHase.
IPR006096. Glu/Leu/Phe/Val_DHase_C.
IPR006097. Glu/Leu/Phe/Val_DHase_dimer.
IPR014362. Glu_DHase.
IPR016040. NAD(P)-bd.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR11606:SF2. GLFV_DH. 1 hit.
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameGUDB_BACSU
AccessionPrimary (citable) accession number: P50735
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 25, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents