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P50694

- TLP_PRUAV

UniProt

P50694 - TLP_PRUAV

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Protein

Glucan endo-1,3-beta-glucosidase

Gene
N/A
Organism
Prunus avium (Cherry) (Cerasus avium)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.1 Publication

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

IgE-binding protein

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Allergen Pru a 2
Beta-1,3-endoglucanase
Thaumatin-like protein
Short name:
TLP
Allergen: Pru av 2
OrganismiPrunus avium (Cherry) (Cerasus avium)
Taxonomic identifieri42229 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeAmygdaleaePrunus

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds IgE in 50% of cherry-allergic patients.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3448. Pru av 2.0101.
598. Pru av 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 245222Glucan endo-1,3-beta-glucosidasePRO_0000034021Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 2441 PublicationPROSITE-ProRule annotation
Disulfide bondi80 ↔ 901 PublicationPROSITE-ProRule annotation
Disulfide bondi95 ↔ 1021 PublicationPROSITE-ProRule annotation
Disulfide bondi150 ↔ 2331 PublicationPROSITE-ProRule annotation
Disulfide bondi155 ↔ 2161 PublicationPROSITE-ProRule annotation
Disulfide bondi163 ↔ 1791 PublicationPROSITE-ProRule annotation
Disulfide bondi183 ↔ 1921 PublicationPROSITE-ProRule annotation
Disulfide bondi193 ↔ 2031 PublicationPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Abundantly expressed in ripening fruit.

Structurei

Secondary structure

1
245
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi25 – 306Combined sources
Beta strandi32 – 343Combined sources
Beta strandi36 – 427Combined sources
Helixi43 – 453Combined sources
Beta strandi61 – 655Combined sources
Beta strandi68 – 8215Combined sources
Beta strandi88 – 936Combined sources
Beta strandi97 – 1015Combined sources
Beta strandi113 – 1186Combined sources
Beta strandi124 – 1307Combined sources
Beta strandi135 – 1373Combined sources
Beta strandi139 – 1457Combined sources
Beta strandi153 – 1553Combined sources
Helixi159 – 1624Combined sources
Helixi165 – 1673Combined sources
Beta strandi168 – 1703Combined sources
Beta strandi176 – 1794Combined sources
Helixi182 – 1865Combined sources
Helixi189 – 1924Combined sources
Turni200 – 2023Combined sources
Helixi207 – 2159Combined sources
Turni226 – 2294Combined sources
Beta strandi231 – 2355Combined sources
Beta strandi238 – 2436Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AHNX-ray1.30A24-245[»]
ProteinModelPortaliP50694.
SMRiP50694. Positions 24-245.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50694.

Family & Domainsi

Sequence similaritiesi

Belongs to the thaumatin family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.110.10. 1 hit.
InterProiIPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view]
PANTHERiPTHR31048. PTHR31048. 1 hit.
PfamiPF00314. Thaumatin. 1 hit.
[Graphical view]
PIRSFiPIRSF002703. Thaumatin. 1 hit.
PRINTSiPR00347. THAUMATIN.
SMARTiSM00205. THN. 1 hit.
[Graphical view]
SUPFAMiSSF49870. SSF49870. 1 hit.
PROSITEiPS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50694-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMKTLVVVLS LSLTILSFGG AHAATISFKN NCPYMVWPGT LTSDQKPQLS
60 70 80 90 100
TTGFELASQA SFQLDTPVPW NGRFWARTGC STDASGKFVC ATADCASGQV
110 120 130 140 150
MCNGNGAIPP ATLAEFNIPA GGGQDFYDVS LVDGFNLPMS VTPQGGTGDC
160 170 180 190 200
KTASCPANVN AVCPSELQKK GSDGSVVACL SACVKFGTPQ YCCTPPQNTP
210 220 230 240
ETCPPTNYSE IFHNACPDAY SYAYDDKRGT FTCNGGPNYA ITFCP
Length:245
Mass (Da):25,707
Last modified:October 1, 1996 - v1
Checksum:iBE045DF46FCEA7CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32440 mRNA. Translation: AAB38064.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U32440 mRNA. Translation: AAB38064.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2AHN X-ray 1.30 A 24-245 [» ]
ProteinModelPortali P50694.
SMRi P50694. Positions 24-245.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3448. Pru av 2.0101.
598. Pru av 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P50694.

Family and domain databases

Gene3Di 2.60.110.10. 1 hit.
InterProi IPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view ]
PANTHERi PTHR31048. PTHR31048. 1 hit.
Pfami PF00314. Thaumatin. 1 hit.
[Graphical view ]
PIRSFi PIRSF002703. Thaumatin. 1 hit.
PRINTSi PR00347. THAUMATIN.
SMARTi SM00205. THN. 1 hit.
[Graphical view ]
SUPFAMi SSF49870. SSF49870. 1 hit.
PROSITEi PS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A cherry protein and its gene, abundantly expressed in ripening fruit, have been identified as thaumatin-like."
    Fils-Lycaon B.R., Wiersma P.A., Eastwell K.C., Sautiere P.
    Plant Physiol. 111:269-273(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Summit.
    Tissue: Fruit.
  2. "Biochemical characterization of Pru a 2, a 23-kD thaumatin-like protein representing a potential major allergen in cherry (Prunus avium)."
    Inschlag C., Hoffmann-Sommergruber K., O'Riordain G., Ahorn H., Ebner C., Scheiner O., Breiteneder H.
    Int. Arch. Allergy Immunol. 116:22-28(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-45, ALLERGEN.
  3. "Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity."
    Fuchs H.C., Bohle B., Dall'Antonia Y., Radauer C., Hoffmann-Sommergruber K., Mari A., Scheiner O., Keller W., Breiteneder H.
    Clin. Exp. Allergy 36:359-368(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYMATIC ACTIVITY.
  4. "Crystallization and preliminary structure determination of the plant food allergen Pru av 2."
    Dall'Antonia Y., Pavkov T., Fuchs H., Breiteneder H., Keller W.
    Acta Crystallogr. F 61:186-188(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 24-245, DISULFIDE BONDS.

Entry informationi

Entry nameiTLP_PRUAV
AccessioniPrimary (citable) accession number: P50694
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Most abundant soluble protein in ripe cherries.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3