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P50694 (TLP_PRUAV) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Allergen Pru a 2
Beta-1,3-endoglucanase
Thaumatin-like protein
Short name=TLP
Allergen=Pru av 2
OrganismPrunus avium (Cherry) (Cerasus avium)
Taxonomic identifier42229 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeAmygdaleaePrunus

Protein attributes

Sequence length245 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans. Ref.3

Subcellular location

Secreted Potential.

Tissue specificity

Abundantly expressed in ripening fruit.

Allergenic properties

Causes an allergic reaction in human. Binds IgE in 50% of cherry-allergic patients. Ref.2

Miscellaneous

Most abundant soluble protein in ripe cherries.

Sequence similarities

Belongs to the thaumatin family.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   DomainSignal
   LigandIgE-binding protein
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucan endo-1,3-beta-D-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.2
Chain24 – 245222Glucan endo-1,3-beta-glucosidase
PRO_0000034021

Amino acid modifications

Disulfide bond32 ↔ 244 Ref.4
Disulfide bond80 ↔ 90 Ref.4
Disulfide bond95 ↔ 102 Ref.4
Disulfide bond150 ↔ 233 Ref.4
Disulfide bond155 ↔ 216 Ref.4
Disulfide bond163 ↔ 179 Ref.4
Disulfide bond183 ↔ 192 Ref.4
Disulfide bond193 ↔ 203 Ref.4

Secondary structure

............................................... 245
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P50694 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: BE045DF46FCEA7CE

FASTA24525,707
        10         20         30         40         50         60 
MMKTLVVVLS LSLTILSFGG AHAATISFKN NCPYMVWPGT LTSDQKPQLS TTGFELASQA 

        70         80         90        100        110        120 
SFQLDTPVPW NGRFWARTGC STDASGKFVC ATADCASGQV MCNGNGAIPP ATLAEFNIPA 

       130        140        150        160        170        180 
GGGQDFYDVS LVDGFNLPMS VTPQGGTGDC KTASCPANVN AVCPSELQKK GSDGSVVACL 

       190        200        210        220        230        240 
SACVKFGTPQ YCCTPPQNTP ETCPPTNYSE IFHNACPDAY SYAYDDKRGT FTCNGGPNYA 


ITFCP 

« Hide

References

[1]"A cherry protein and its gene, abundantly expressed in ripening fruit, have been identified as thaumatin-like."
Fils-Lycaon B.R., Wiersma P.A., Eastwell K.C., Sautiere P.
Plant Physiol. 111:269-273(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Summit.
Tissue: Fruit.
[2]"Biochemical characterization of Pru a 2, a 23-kD thaumatin-like protein representing a potential major allergen in cherry (Prunus avium)."
Inschlag C., Hoffmann-Sommergruber K., O'Riordain G., Ahorn H., Ebner C., Scheiner O., Breiteneder H.
Int. Arch. Allergy Immunol. 116:22-28(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-45, ALLERGEN.
[3]"Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity."
Fuchs H.C., Bohle B., Dall'Antonia Y., Radauer C., Hoffmann-Sommergruber K., Mari A., Scheiner O., Keller W., Breiteneder H.
Clin. Exp. Allergy 36:359-368(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYMATIC ACTIVITY.
[4]"Crystallization and preliminary structure determination of the plant food allergen Pru av 2."
Dall'Antonia Y., Pavkov T., Fuchs H., Breiteneder H., Keller W.
Acta Crystallogr. F 61:186-188(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 24-245, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U32440 mRNA. Translation: AAB38064.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AHNX-ray1.30A24-245[»]
ProteinModelPortalP50694.
SMRP50694. Positions 24-245.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome3448. Pru av 2.0101.
598. Pru av 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.110.10. 1 hit.
InterProIPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view]
PfamPF00314. Thaumatin. 1 hit.
[Graphical view]
PIRSFPIRSF002703. Thaumatin. 1 hit.
PRINTSPR00347. THAUMATIN.
SMARTSM00205. THN. 1 hit.
[Graphical view]
SUPFAMSSF49870. SSF49870. 1 hit.
PROSITEPS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP50694.

Entry information

Entry nameTLP_PRUAV
AccessionPrimary (citable) accession number: P50694
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Allergens

Nomenclature of allergens and list of entries