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Protein

Glucan endo-1,3-beta-glucosidase

Gene
N/A
Organism
Prunus avium (Cherry) (Cerasus avium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

IgE-binding protein

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Allergen Pru a 2
Beta-1,3-endoglucanase
Thaumatin-like protein
Short name:
TLP
Allergen: Pru av 2
OrganismiPrunus avium (Cherry) (Cerasus avium)
Taxonomic identifieri42229 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeAmygdaleaePrunus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds IgE in 50% of cherry-allergic patients.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3448. Pru av 2.0101.
598. Pru av 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000003402124 – 245Glucan endo-1,3-beta-glucosidaseAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 244PROSITE-ProRule annotation1 Publication
Disulfide bondi80 ↔ 90PROSITE-ProRule annotation1 Publication
Disulfide bondi95 ↔ 102PROSITE-ProRule annotation1 Publication
Disulfide bondi150 ↔ 233PROSITE-ProRule annotation1 Publication
Disulfide bondi155 ↔ 216PROSITE-ProRule annotation1 Publication
Disulfide bondi163 ↔ 179PROSITE-ProRule annotation1 Publication
Disulfide bondi183 ↔ 192PROSITE-ProRule annotation1 Publication
Disulfide bondi193 ↔ 203PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Abundantly expressed in ripening fruit.

Structurei

Secondary structure

1245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 30Combined sources6
Beta strandi32 – 34Combined sources3
Beta strandi36 – 42Combined sources7
Helixi43 – 45Combined sources3
Beta strandi61 – 65Combined sources5
Beta strandi68 – 82Combined sources15
Beta strandi88 – 93Combined sources6
Beta strandi97 – 101Combined sources5
Beta strandi113 – 118Combined sources6
Beta strandi124 – 130Combined sources7
Beta strandi135 – 137Combined sources3
Beta strandi139 – 145Combined sources7
Beta strandi153 – 155Combined sources3
Helixi159 – 162Combined sources4
Helixi165 – 167Combined sources3
Beta strandi168 – 170Combined sources3
Beta strandi176 – 179Combined sources4
Helixi182 – 186Combined sources5
Helixi189 – 192Combined sources4
Turni200 – 202Combined sources3
Helixi207 – 215Combined sources9
Turni226 – 229Combined sources4
Beta strandi231 – 235Combined sources5
Beta strandi238 – 243Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AHNX-ray1.30A24-245[»]
ProteinModelPortaliP50694.
SMRiP50694.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50694.

Family & Domainsi

Sequence similaritiesi

Belongs to the thaumatin family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.110.10. 1 hit.
InterProiIPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view]
PANTHERiPTHR31048. PTHR31048. 1 hit.
PfamiPF00314. Thaumatin. 1 hit.
[Graphical view]
PIRSFiPIRSF002703. Thaumatin. 1 hit.
PRINTSiPR00347. THAUMATIN.
SMARTiSM00205. THN. 1 hit.
[Graphical view]
SUPFAMiSSF49870. SSF49870. 1 hit.
PROSITEiPS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50694-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKTLVVVLS LSLTILSFGG AHAATISFKN NCPYMVWPGT LTSDQKPQLS
60 70 80 90 100
TTGFELASQA SFQLDTPVPW NGRFWARTGC STDASGKFVC ATADCASGQV
110 120 130 140 150
MCNGNGAIPP ATLAEFNIPA GGGQDFYDVS LVDGFNLPMS VTPQGGTGDC
160 170 180 190 200
KTASCPANVN AVCPSELQKK GSDGSVVACL SACVKFGTPQ YCCTPPQNTP
210 220 230 240
ETCPPTNYSE IFHNACPDAY SYAYDDKRGT FTCNGGPNYA ITFCP
Length:245
Mass (Da):25,707
Last modified:October 1, 1996 - v1
Checksum:iBE045DF46FCEA7CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32440 mRNA. Translation: AAB38064.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32440 mRNA. Translation: AAB38064.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AHNX-ray1.30A24-245[»]
ProteinModelPortaliP50694.
SMRiP50694.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3448. Pru av 2.0101.
598. Pru av 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP50694.

Family and domain databases

Gene3Di2.60.110.10. 1 hit.
InterProiIPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view]
PANTHERiPTHR31048. PTHR31048. 1 hit.
PfamiPF00314. Thaumatin. 1 hit.
[Graphical view]
PIRSFiPIRSF002703. Thaumatin. 1 hit.
PRINTSiPR00347. THAUMATIN.
SMARTiSM00205. THN. 1 hit.
[Graphical view]
SUPFAMiSSF49870. SSF49870. 1 hit.
PROSITEiPS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLP_PRUAV
AccessioniPrimary (citable) accession number: P50694
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Most abundant soluble protein in ripe cherries.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.