Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P50694

- TLP_PRUAV

UniProt

P50694 - TLP_PRUAV

Protein

Glucan endo-1,3-beta-glucosidase

Gene
N/A
Organism
Prunus avium (Cherry) (Cerasus avium)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.1 Publication

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    IgE-binding protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase
    Short name:
    (1->3)-beta-glucanase
    Allergen Pru a 2
    Beta-1,3-endoglucanase
    Thaumatin-like protein
    Short name:
    TLP
    Allergen: Pru av 2
    OrganismiPrunus avium (Cherry) (Cerasus avium)
    Taxonomic identifieri42229 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeAmygdaleaePrunus

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. Binds IgE in 50% of cherry-allergic patients.1 Publication

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei3448. Pru av 2.0101.
    598. Pru av 2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 245222Glucan endo-1,3-beta-glucosidasePRO_0000034021Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 2441 PublicationPROSITE-ProRule annotation
    Disulfide bondi80 ↔ 901 PublicationPROSITE-ProRule annotation
    Disulfide bondi95 ↔ 1021 PublicationPROSITE-ProRule annotation
    Disulfide bondi150 ↔ 2331 PublicationPROSITE-ProRule annotation
    Disulfide bondi155 ↔ 2161 PublicationPROSITE-ProRule annotation
    Disulfide bondi163 ↔ 1791 PublicationPROSITE-ProRule annotation
    Disulfide bondi183 ↔ 1921 PublicationPROSITE-ProRule annotation
    Disulfide bondi193 ↔ 2031 PublicationPROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Abundantly expressed in ripening fruit.

    Structurei

    Secondary structure

    1
    245
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi25 – 306
    Beta strandi32 – 343
    Beta strandi36 – 427
    Helixi43 – 453
    Beta strandi61 – 655
    Beta strandi68 – 8215
    Beta strandi88 – 936
    Beta strandi97 – 1015
    Beta strandi113 – 1186
    Beta strandi124 – 1307
    Beta strandi135 – 1373
    Beta strandi139 – 1457
    Beta strandi153 – 1553
    Helixi159 – 1624
    Helixi165 – 1673
    Beta strandi168 – 1703
    Beta strandi176 – 1794
    Helixi182 – 1865
    Helixi189 – 1924
    Turni200 – 2023
    Helixi207 – 2159
    Turni226 – 2294
    Beta strandi231 – 2355
    Beta strandi238 – 2436

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AHNX-ray1.30A24-245[»]
    ProteinModelPortaliP50694.
    SMRiP50694. Positions 24-245.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP50694.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thaumatin family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.110.10. 1 hit.
    InterProiIPR001938. Thaumatin.
    IPR017949. Thaumatin_CS.
    [Graphical view]
    PfamiPF00314. Thaumatin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002703. Thaumatin. 1 hit.
    PRINTSiPR00347. THAUMATIN.
    SMARTiSM00205. THN. 1 hit.
    [Graphical view]
    SUPFAMiSSF49870. SSF49870. 1 hit.
    PROSITEiPS00316. THAUMATIN_1. 1 hit.
    PS51367. THAUMATIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50694-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMKTLVVVLS LSLTILSFGG AHAATISFKN NCPYMVWPGT LTSDQKPQLS    50
    TTGFELASQA SFQLDTPVPW NGRFWARTGC STDASGKFVC ATADCASGQV 100
    MCNGNGAIPP ATLAEFNIPA GGGQDFYDVS LVDGFNLPMS VTPQGGTGDC 150
    KTASCPANVN AVCPSELQKK GSDGSVVACL SACVKFGTPQ YCCTPPQNTP 200
    ETCPPTNYSE IFHNACPDAY SYAYDDKRGT FTCNGGPNYA ITFCP 245
    Length:245
    Mass (Da):25,707
    Last modified:October 1, 1996 - v1
    Checksum:iBE045DF46FCEA7CE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32440 mRNA. Translation: AAB38064.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U32440 mRNA. Translation: AAB38064.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2AHN X-ray 1.30 A 24-245 [» ]
    ProteinModelPortali P50694.
    SMRi P50694. Positions 24-245.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 3448. Pru av 2.0101.
    598. Pru av 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P50694.

    Family and domain databases

    Gene3Di 2.60.110.10. 1 hit.
    InterProi IPR001938. Thaumatin.
    IPR017949. Thaumatin_CS.
    [Graphical view ]
    Pfami PF00314. Thaumatin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002703. Thaumatin. 1 hit.
    PRINTSi PR00347. THAUMATIN.
    SMARTi SM00205. THN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49870. SSF49870. 1 hit.
    PROSITEi PS00316. THAUMATIN_1. 1 hit.
    PS51367. THAUMATIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A cherry protein and its gene, abundantly expressed in ripening fruit, have been identified as thaumatin-like."
      Fils-Lycaon B.R., Wiersma P.A., Eastwell K.C., Sautiere P.
      Plant Physiol. 111:269-273(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Summit.
      Tissue: Fruit.
    2. "Biochemical characterization of Pru a 2, a 23-kD thaumatin-like protein representing a potential major allergen in cherry (Prunus avium)."
      Inschlag C., Hoffmann-Sommergruber K., O'Riordain G., Ahorn H., Ebner C., Scheiner O., Breiteneder H.
      Int. Arch. Allergy Immunol. 116:22-28(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-45, ALLERGEN.
    3. "Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity."
      Fuchs H.C., Bohle B., Dall'Antonia Y., Radauer C., Hoffmann-Sommergruber K., Mari A., Scheiner O., Keller W., Breiteneder H.
      Clin. Exp. Allergy 36:359-368(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYMATIC ACTIVITY.
    4. "Crystallization and preliminary structure determination of the plant food allergen Pru av 2."
      Dall'Antonia Y., Pavkov T., Fuchs H., Breiteneder H., Keller W.
      Acta Crystallogr. F 61:186-188(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 24-245, DISULFIDE BONDS.

    Entry informationi

    Entry nameiTLP_PRUAV
    AccessioniPrimary (citable) accession number: P50694
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Most abundant soluble protein in ripe cherries.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3