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Protein

Ribonucleoside-diphosphate reductase small chain A

Gene

RNR2A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.2 Publications

Miscellaneous

A substrate-binding catalytic site, located on R1, is formed only in the presence of the second subunit R2.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cation1 PublicationNote: Binds 2 iron ions per subunit.1 Publication

Enzyme regulationi

Inhibited by phenol, paracetamol, 2,4,6-trimethylphenol, resveratrol, furfuryl mercaptan, 2-thiophenthiol, phenylhydrazine, and hydroxyurea.1 Publication

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi87Iron 1PROSITE-ProRule annotation1
Metal bindingi118Iron 1PROSITE-ProRule annotation1
Metal bindingi118Iron 2By similarity1
Metal bindingi121Iron 1PROSITE-ProRule annotation1
Active sitei125PROSITE-ProRule annotation1
Metal bindingi180Iron 2By similarity1
Metal bindingi214Iron 2By similarity1
Metal bindingi217Iron 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • deoxyribonucleotide biosynthetic process Source: InterPro
  • DNA repair Source: TAIR
  • DNA replication Source: UniProtKB-UniPathway
  • multicellular organism development Source: TAIR
  • regulation of cell cycle Source: TAIR
  • ribonucleotide metabolic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processDNA replication
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G23580-MONOMER
MetaCyc:AT3G23580-MONOMER
BRENDAi1.17.4.1 399
1.17.4.2 399
ReactomeiR-ATH-499943 Interconversion of nucleotide di- and triphosphates
UniPathwayiUPA00326

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small chain A (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase R2A subunit
Short name:
AtRNR2
Short name:
Protein R2at
Ribonucleotide reductase small subunit A
Gene namesi
Name:RNR2A
Synonyms:RNR2
Ordered Locus Names:At3g23580
ORF Names:MDB19.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G23580
TAIRilocus:2088040 AT3G23580

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, due to the redundancy with RNR2B and TSO2. Rnr2a and rnr2b double mutants have no visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001904661 – 341Ribonucleoside-diphosphate reductase small chain AAdd BLAST341

Proteomic databases

PaxDbiP50651
PRIDEiP50651

Expressioni

Tissue specificityi

Expressed in rosette leaves, cauline leaves, stems and flowers.2 Publications

Developmental stagei

Accumulated during S phase of the cell cycle.1 Publication

Gene expression databases

ExpressionAtlasiP50651 baseline and differential
GenevisibleiP50651 AT

Interactioni

Subunit structurei

Homodimer and heterodimer with TSO2. Heterotetramer of two R1 and two R2 chains. A radical transfer pathway may occur between Tyr-125 of protein R2 and R1. Homodimer contains a dinuclear non-heme iron center and a stable tyrosyl radical essential for activity. A transfer pathway may occur between Tyr-125 of protein R2 and R1. Interacts with CSN7.2 Publications

Protein-protein interaction databases

BioGridi7269, 1 interactor
STRINGi3702.AT3G23580.1

Structurei

3D structure databases

ProteinModelPortaliP50651
SMRiP50651
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1567 Eukaryota
COG0208 LUCA
HOGENOMiHOG000255975
InParanoidiP50651
KOiK10808
OMAiFYLPLHF
OrthoDBiEOG09360F7K
PhylomeDBiP50651

Family and domain databases

CDDicd01049 RNRR2, 1 hit
Gene3Di1.10.620.20, 1 hit
InterProiView protein in InterPro
IPR009078 Ferritin-like_SF
IPR012348 RNR-like
IPR033909 RNR_small
IPR030475 RNR_small_AS
IPR000358 RNR_small_fam
PANTHERiPTHR23409 PTHR23409, 1 hit
PfamiView protein in Pfam
PF00268 Ribonuc_red_sm, 1 hit
PIRSFiPIRSF000355 NrdB, 1 hit
SUPFAMiSSF47240 SSF47240, 1 hit
PROSITEiView protein in PROSITE
PS00368 RIBORED_SMALL, 1 hit

Sequencei

Sequence statusi: Complete.

P50651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLKEGQGR DMEEGESEEP LLMAQNQRFT MFPIRYKSIW EMYKKAEASF
60 70 80 90 100
WTAEEVDLST DVQQWEALTD SEKHFISHIL AFFAASDGIV LENLAARFLN
110 120 130 140 150
DVQVPEARAF YGFQIAMENI HSEMYSLLLE TFIKDSKEKD RLFNAIETIP
160 170 180 190 200
CISKKAKWCL DWIQSPMSFA VRLVAFACVE GIFFSGSFCA IFWLKKRGLM
210 220 230 240 250
PGLTFSNELI SRDEGLHCDF ACLLYSLLQK QLPLEKVYQI VHEAVEIETE
260 270 280 290 300
FVCKALPCDL IGMNSNLMSQ YIQFVADRLL VTLGCERTYK AENPFDWMEF
310 320 330 340
ISLQGKTNFF EKRVGEYQKA SVMSNLQNGN QNYEFTTEED F
Length:341
Mass (Da):39,370
Last modified:January 10, 2003 - v2
Checksum:i3214A88AE7B7776A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95Missing in CAA54549 (PubMed:7821432).Curated1
Sequence conflicti179V → A in CAA54549 (PubMed:7821432).Curated1
Sequence conflicti225Y → I in CAA54549 (PubMed:7821432).Curated1
Sequence conflicti230 – 232KQL → LHV in CAA54549 (PubMed:7821432).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77336 mRNA Translation: CAA54549.1
AB023036 Genomic DNA Translation: BAB02776.1
CP002686 Genomic DNA Translation: AEE76779.1
AF372971 mRNA Translation: AAK50108.1
AY143893 mRNA Translation: AAN28832.1
PIRiS68538
RefSeqiNP_189000.1, NM_113261.4
UniGeneiAt.8049

Genome annotation databases

EnsemblPlantsiAT3G23580.1; AT3G23580.1; AT3G23580
GeneIDi821937
GrameneiAT3G23580.1; AT3G23580.1; AT3G23580
KEGGiath:AT3G23580

Similar proteinsi

Entry informationi

Entry nameiRIR2A_ARATH
AccessioniPrimary (citable) accession number: P50651
Secondary accession number(s): Q9LUH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 10, 2003
Last modified: April 25, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health