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Protein

Ribonucleoside-diphosphate reductase small subunit

Gene

RNR2

Organism
Plasmodium falciparum (isolate FCR-3 / Gambia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 1PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron 2By similarity
Metal bindingi104 – 1041Iron 1PROSITE-ProRule annotation
Active sitei108 – 1081PROSITE-ProRule annotation
Metal bindingi163 – 1631Iron 2By similarity
Metal bindingi197 – 1971Iron 2By similarity
Metal bindingi200 – 2001Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase R2 subunit
Ribonucleotide reductase small subunit
Gene namesi
Name:RNR2
OrganismiPlasmodium falciparum (isolate FCR-3 / Gambia)
Taxonomic identifieri5838 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Ribonucleoside-diphosphate reductase small subunitPRO_0000190458Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a large and a small subunit.

Structurei

3D structure databases

ProteinModelPortaliP50649.
SMRiP50649. Positions 3-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRILNKESD RFTLYPILYP DVFPFYKKAE ACFWTAEEID YSSDLKDFEK
60 70 80 90 100
LNENEKHFIK HVLAFFAASD GIVLENLAVS FLREVQITEA KKFYSFQIAV
110 120 130 140 150
ENIHSETYSL LIDNYIKDEK ERLNLFHAIE NIPAVKNKAL WAAKWINDTN
160 170 180 190 200
SFAERIVANA CVEGILFSGS FCAIFWFKKQ NKLHGLTFSN ELISRDEGLH
210 220 230 240 250
TDFNCLIYSL LDNKLPEQMV QNIVKEAGGV EVEKSFICES LPCDLIGMNS
260 270 280 290 300
RLMSQYIEFV ADRLLECLGC SKIFHSKNPF NWMDLISLQG KTNFFEKRVA
310 320
DYQKSGVMAQ RKDHVFCLNT EF
Length:322
Mass (Da):37,372
Last modified:October 1, 1996 - v1
Checksum:i73AD1257E294A013
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22058 Genomic DNA. Translation: AAA29754.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22058 Genomic DNA. Translation: AAA29754.1.

3D structure databases

ProteinModelPortaliP50649.
SMRiP50649. Positions 3-285.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, sequence determination, and regulation of the ribonucleotide reductase subunits from Plasmodium falciparum: a target for antimalarial therapy."
    Rubin H., Salem J.S., Li L.S., Yang F.D., Mama S., Wang Z.M., Fisher A., Hamann C.S., Cooperman B.S.
    Proc. Natl. Acad. Sci. U.S.A. 90:9280-9284(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiRIR2_PLAFG
AccessioniPrimary (citable) accession number: P50649
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 1, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.