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P50646

- RIR1_BHV1C

UniProt

P50646 - RIR1_BHV1C

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Protein
Ribonucleoside-diphosphate reductase large subunit
Gene
UL39
Organism
Bovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei209 – 2091Substrate By similarity
Sitei225 – 2251Important for hydrogen atom transfer By similarity
Binding sitei255 – 2551Substrate; via amide nitrogen By similarity
Active sitei436 – 4361Proton acceptor By similarity
Active sitei438 – 4381Cysteine radical intermediate By similarity
Active sitei440 – 4401Proton acceptor By similarity
Sitei453 – 4531Important for hydrogen atom transfer By similarity
Sitei762 – 7621Important for electron transfer By similarity
Sitei763 – 7631Important for electron transfer By similarity
Sitei782 – 7821Interacts with thioredoxin/glutaredoxin By similarity
Sitei785 – 7851Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Name:UL39
OrganismiBovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)
Taxonomic identifieri10323 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 787787Ribonucleoside-diphosphate reductase large subunit
PRO_0000187240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi225 ↔ 453Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit UL39 (R1) and a homodimer of the small subunit UL40 (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Structurei

3D structure databases

ProteinModelPortaliP50646.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni224 – 2252Substrate binding By similarity
Regioni436 – 4405Substrate binding By similarity
Regioni618 – 6225Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50646-1 [UniParc]FASTAAdd to Basket

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MASDAFMQTA CPADAAEQLE AEHAEWAQLG CGAVPPPPAA ASRPSRAAVA    50
AYVGEVVDRM RAQSRADERV YVKCGQLVHL RVRARSVPLD DWLTSAELAL 100
VSEVAEPVRA NRAFVEVSLR YFELTEYATL RALGLQSALK YEEMYLAKLE 150
GGAIESMGQF FVRIAATAAT WTMREPAFGR ALVGEGATWC AVFNAYLTAL 200
YRQLVVPATP IMLFAGRARG SLASCYLLNP QVSSSTEAVE AITTEVARIL 250
LNRGGIGISF QSFDRAVSRD CKRGIMGALK LLDSMAMAIN SDSERPTGIC 300
VYLEPWHCDV RAVLNMRGLL ARDESTRCDN LFSCLWVPDL LFDRYLAHLE 350
GREGVVWTLF DDRASHLSRL HGPAFTAEYE RLEREGLGVE TVPVQDLAFL 400
IVRSIVMTGS PFVMFKDACN RHYHMDTAGD ALTGSNLCTE IVQRASPDAH 450
GVCNLASVNL PRCVREGEGG ALAFDFAALS TAAATAAIFV NAMMLGGQYP 500
TEKAARGVAR HRSLGIGFQG LHTLLLELGM DMLSPAARRL NVEIAERLLL 550
AVMATSATLC EYGCAPFEDF ARSKFARGLM PFDGYEGVVL SLPRAWARLR 600
EKVARHGLYN AQFVALMPTV SSSQVTEGSE GFSPVFTNMF SKVTMSGELL 650
RPNLPLMRAL RKHFTREASR LGAVRALDRE QWSVAAALGD LAPGHPLAKF 700
KTAFEYDQER LIDLCADRAP FVDQSQSMSL FVTEPMDGKV PASQIMNLLV 750
YAYKKGLKTG LYYCKIRKAT NNGVFTGGDL VCSGCHL 787
Length:787
Mass (Da):86,010
Last modified:October 1, 1996 - v1
Checksum:i8DB0F6327862333E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z54206 Genomic DNA. Translation: CAA90929.1.
Z49078 Genomic DNA. Translation: CAA88900.1.
AJ004801 Genomic DNA. Translation: CAA06094.1.
RefSeqiNP_045319.1. NC_001847.1.

Genome annotation databases

GeneIDi1487390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z54206 Genomic DNA. Translation: CAA90929.1 .
Z49078 Genomic DNA. Translation: CAA88900.1 .
AJ004801 Genomic DNA. Translation: CAA06094.1 .
RefSeqi NP_045319.1. NC_001847.1.

3D structure databases

ProteinModelPortali P50646.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1487390.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the UL39, UL38, and UL37 homologues of bovine herpesvirus 1 and expression studies of UL40 and UL39, the subunits of ribonucleotide reductase."
    Simard C., Langlois I., Styger D., Vogt B., Vlcek C., Chalifour A., Trudel M., Schwyzer M.
    Virology 212:734-740(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Gene contents in a 31-kb segment at the left genome end of bovine herpesvirus-1."
    Schwyzer M., Styger D., Vogt B., Lowery D.E., Simard C., LaBoissiere S., Misra V., Vlcek C., Paces V.
    Vet. Microbiol. 53:67-77(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_BHV1C
AccessioniPrimary (citable) accession number: P50646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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