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P50646

- RIR1_BHV1C

UniProt

P50646 - RIR1_BHV1C

Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

UL39

Organism
Bovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei209 – 2091SubstrateBy similarity
    Sitei225 – 2251Important for hydrogen atom transferBy similarity
    Binding sitei255 – 2551Substrate; via amide nitrogenBy similarity
    Active sitei436 – 4361Proton acceptorBy similarity
    Active sitei438 – 4381Cysteine radical intermediateBy similarity
    Active sitei440 – 4401Proton acceptorBy similarity
    Sitei453 – 4531Important for hydrogen atom transferBy similarity
    Sitei762 – 7621Important for electron transferBy similarity
    Sitei763 – 7631Important for electron transferBy similarity
    Sitei782 – 7821Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei785 – 7851Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase large subunit
    Gene namesi
    Name:UL39
    OrganismiBovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)
    Taxonomic identifieri10323 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 787787Ribonucleoside-diphosphate reductase large subunitPRO_0000187240Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi225 ↔ 453Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit UL39 (R1) and a homodimer of the small subunit UL40 (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP50646.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni224 – 2252Substrate bindingBy similarity
    Regioni436 – 4405Substrate bindingBy similarity
    Regioni618 – 6225Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view]
    PfamiPF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50646-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASDAFMQTA CPADAAEQLE AEHAEWAQLG CGAVPPPPAA ASRPSRAAVA    50
    AYVGEVVDRM RAQSRADERV YVKCGQLVHL RVRARSVPLD DWLTSAELAL 100
    VSEVAEPVRA NRAFVEVSLR YFELTEYATL RALGLQSALK YEEMYLAKLE 150
    GGAIESMGQF FVRIAATAAT WTMREPAFGR ALVGEGATWC AVFNAYLTAL 200
    YRQLVVPATP IMLFAGRARG SLASCYLLNP QVSSSTEAVE AITTEVARIL 250
    LNRGGIGISF QSFDRAVSRD CKRGIMGALK LLDSMAMAIN SDSERPTGIC 300
    VYLEPWHCDV RAVLNMRGLL ARDESTRCDN LFSCLWVPDL LFDRYLAHLE 350
    GREGVVWTLF DDRASHLSRL HGPAFTAEYE RLEREGLGVE TVPVQDLAFL 400
    IVRSIVMTGS PFVMFKDACN RHYHMDTAGD ALTGSNLCTE IVQRASPDAH 450
    GVCNLASVNL PRCVREGEGG ALAFDFAALS TAAATAAIFV NAMMLGGQYP 500
    TEKAARGVAR HRSLGIGFQG LHTLLLELGM DMLSPAARRL NVEIAERLLL 550
    AVMATSATLC EYGCAPFEDF ARSKFARGLM PFDGYEGVVL SLPRAWARLR 600
    EKVARHGLYN AQFVALMPTV SSSQVTEGSE GFSPVFTNMF SKVTMSGELL 650
    RPNLPLMRAL RKHFTREASR LGAVRALDRE QWSVAAALGD LAPGHPLAKF 700
    KTAFEYDQER LIDLCADRAP FVDQSQSMSL FVTEPMDGKV PASQIMNLLV 750
    YAYKKGLKTG LYYCKIRKAT NNGVFTGGDL VCSGCHL 787
    Length:787
    Mass (Da):86,010
    Last modified:October 1, 1996 - v1
    Checksum:i8DB0F6327862333E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54206 Genomic DNA. Translation: CAA90929.1.
    Z49078 Genomic DNA. Translation: CAA88900.1.
    AJ004801 Genomic DNA. Translation: CAA06094.1.
    RefSeqiNP_045319.1. NC_001847.1.

    Genome annotation databases

    GeneIDi1487390.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54206 Genomic DNA. Translation: CAA90929.1 .
    Z49078 Genomic DNA. Translation: CAA88900.1 .
    AJ004801 Genomic DNA. Translation: CAA06094.1 .
    RefSeqi NP_045319.1. NC_001847.1.

    3D structure databases

    ProteinModelPortali P50646.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1487390.

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    InterProi IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view ]
    Pfami PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the UL39, UL38, and UL37 homologues of bovine herpesvirus 1 and expression studies of UL40 and UL39, the subunits of ribonucleotide reductase."
      Simard C., Langlois I., Styger D., Vogt B., Vlcek C., Chalifour A., Trudel M., Schwyzer M.
      Virology 212:734-740(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Gene contents in a 31-kb segment at the left genome end of bovine herpesvirus-1."
      Schwyzer M., Styger D., Vogt B., Lowery D.E., Simard C., LaBoissiere S., Misra V., Vlcek C., Paces V.
      Vet. Microbiol. 53:67-77(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Tinkering with a viral ribonucleotide reductase."
      Lembo D., Brune W.
      Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiRIR1_BHV1C
    AccessioniPrimary (citable) accession number: P50646
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3