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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RIR1

Organism
Bovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei209SubstrateUniRule annotation1
Sitei225Important for hydrogen atom transferUniRule annotation1
Binding sitei255Substrate; via amide nitrogenUniRule annotation1
Active sitei436Proton acceptorUniRule annotation1
Active sitei438Cysteine radical intermediateUniRule annotation1
Active sitei440Proton acceptorUniRule annotation1
Sitei453Important for hydrogen atom transferUniRule annotation1
Sitei762Important for electron transferUniRule annotation1
Sitei763Important for electron transferUniRule annotation1
Sitei782Interacts with thioredoxin/glutaredoxinUniRule annotation1
Sitei785Interacts with thioredoxin/glutaredoxinUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:RIR1UniRule annotation
Ordered Locus Names:UL39
OrganismiBovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus)
Taxonomic identifieri10323 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872401 – 787Ribonucleoside-diphosphate reductase large subunitAdd BLAST787

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi225 ↔ 453Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP50646.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni224 – 225Substrate bindingUniRule annotation2
Regioni436 – 440Substrate bindingUniRule annotation5
Regioni618 – 622Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Phylogenomic databases

KOiK00525.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASDAFMQTA CPADAAEQLE AEHAEWAQLG CGAVPPPPAA ASRPSRAAVA
60 70 80 90 100
AYVGEVVDRM RAQSRADERV YVKCGQLVHL RVRARSVPLD DWLTSAELAL
110 120 130 140 150
VSEVAEPVRA NRAFVEVSLR YFELTEYATL RALGLQSALK YEEMYLAKLE
160 170 180 190 200
GGAIESMGQF FVRIAATAAT WTMREPAFGR ALVGEGATWC AVFNAYLTAL
210 220 230 240 250
YRQLVVPATP IMLFAGRARG SLASCYLLNP QVSSSTEAVE AITTEVARIL
260 270 280 290 300
LNRGGIGISF QSFDRAVSRD CKRGIMGALK LLDSMAMAIN SDSERPTGIC
310 320 330 340 350
VYLEPWHCDV RAVLNMRGLL ARDESTRCDN LFSCLWVPDL LFDRYLAHLE
360 370 380 390 400
GREGVVWTLF DDRASHLSRL HGPAFTAEYE RLEREGLGVE TVPVQDLAFL
410 420 430 440 450
IVRSIVMTGS PFVMFKDACN RHYHMDTAGD ALTGSNLCTE IVQRASPDAH
460 470 480 490 500
GVCNLASVNL PRCVREGEGG ALAFDFAALS TAAATAAIFV NAMMLGGQYP
510 520 530 540 550
TEKAARGVAR HRSLGIGFQG LHTLLLELGM DMLSPAARRL NVEIAERLLL
560 570 580 590 600
AVMATSATLC EYGCAPFEDF ARSKFARGLM PFDGYEGVVL SLPRAWARLR
610 620 630 640 650
EKVARHGLYN AQFVALMPTV SSSQVTEGSE GFSPVFTNMF SKVTMSGELL
660 670 680 690 700
RPNLPLMRAL RKHFTREASR LGAVRALDRE QWSVAAALGD LAPGHPLAKF
710 720 730 740 750
KTAFEYDQER LIDLCADRAP FVDQSQSMSL FVTEPMDGKV PASQIMNLLV
760 770 780
YAYKKGLKTG LYYCKIRKAT NNGVFTGGDL VCSGCHL
Length:787
Mass (Da):86,010
Last modified:October 1, 1996 - v1
Checksum:i8DB0F6327862333E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54206 Genomic DNA. Translation: CAA90929.1.
Z49078 Genomic DNA. Translation: CAA88900.1.
AJ004801 Genomic DNA. Translation: CAA06094.1.
RefSeqiNP_045319.1. NC_001847.1.

Genome annotation databases

GeneIDi1487390.
KEGGivg:1487390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54206 Genomic DNA. Translation: CAA90929.1.
Z49078 Genomic DNA. Translation: CAA88900.1.
AJ004801 Genomic DNA. Translation: CAA06094.1.
RefSeqiNP_045319.1. NC_001847.1.

3D structure databases

ProteinModelPortaliP50646.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1487390.
KEGGivg:1487390.

Phylogenomic databases

KOiK00525.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_BHV1C
AccessioniPrimary (citable) accession number: P50646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.