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Protein

Ribonucleoside-diphosphate reductase small subunit

Gene

RIR2

Organism
Suid herpesvirus 1 (strain Kaplan) (SuHV-1) (Pseudorabies virus (strain Kaplan))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Iron 1UniRule annotation
Metal bindingi90 – 901Iron 1UniRule annotation
Metal bindingi90 – 901Iron 2UniRule annotation
Metal bindingi93 – 931Iron 1UniRule annotation
Active sitei97 – 971UniRule annotation
Metal bindingi153 – 1531Iron 2UniRule annotation
Metal bindingi187 – 1871Iron 2UniRule annotation
Metal bindingi190 – 1901Iron 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Alternative name(s):
Ribonucleotide reductase small subunitUniRule annotation
Gene namesi
Name:RIR2UniRule annotation
Synonyms:RR2, UL40
OrganismiSuid herpesvirus 1 (strain Kaplan) (SuHV-1) (Pseudorabies virus (strain Kaplan))
Taxonomic identifieri33703 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Host membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei147 – 16721HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Ribonucleoside-diphosphate reductase small subunitPRO_0000190511Add
BLAST

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP50645.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase small chain family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK10808.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
HAMAPiMF_04028. HSV_RIR2.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYFYTSQCP DMDHLRSLSV ANRWLETDLP LGDDAKDVAA LSEPELEFYR
60 70 80 90 100
FLFAFLSAAD DLVNVNLGSL SELFTQKDIL HYYIEQECIE VVHSRVYSAI
110 120 130 140 150
QLMLFRGDAA ARERYVRAAL RDEAIRRKVE WLDSRVAECA SVAEKYLLMI
160 170 180 190 200
LIEGIFFASS FASISYLRTH NLFVVTCQSN DFISRDEAIH TSASCCIYNN
210 220 230 240 250
YLGDAPRPDE ARIHQLFAEA VEIECEFLRA RAPRDSLLLD LPAIISYVRY
260 270 280 290 300
SADRLLQAIG ASPLFGAPAP AADFPMALMV AEKHTNFFER RSTNYTGTVV

NDL
Length:303
Mass (Da):34,375
Last modified:October 1, 1996 - v1
Checksum:i560F19FDE6C7A9C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72087 Genomic DNA. Translation: CAA50977.1.
X80797 Genomic DNA. Translation: CAA56776.1.
PIRiS47526.
RefSeqiYP_068343.1. NC_006151.1.

Genome annotation databases

GeneIDi2952536.
KEGGivg:2952536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72087 Genomic DNA. Translation: CAA50977.1.
X80797 Genomic DNA. Translation: CAA56776.1.
PIRiS47526.
RefSeqiYP_068343.1. NC_006151.1.

3D structure databases

ProteinModelPortaliP50645.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2952536.
KEGGivg:2952536.

Phylogenomic databases

KOiK10808.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
HAMAPiMF_04028. HSV_RIR2.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
IPR030475. RNR_small_AS.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Large and small subunits of the Aujeszky's disease virus ribonucleotide reductase: nucleotide sequence and putative structure."
    Kaliman A., Boldogkoi Z., Fodor I.
    Biochim. Biophys. Acta 1219:151-156(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR2_SUHVK
AccessioniPrimary (citable) accession number: P50645
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.