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Protein

Ribonucleoside-diphosphate reductase small subunit

Gene

RIR2

Organism
Equine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Iron 1UniRule annotation1
Metal bindingi107Iron 1UniRule annotation1
Metal bindingi107Iron 2UniRule annotation1
Metal bindingi110Iron 1UniRule annotation1
Active sitei114UniRule annotation1
Metal bindingi170Iron 2UniRule annotation1
Metal bindingi204Iron 2UniRule annotation1
Metal bindingi207Iron 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase small subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Alternative name(s):
Ribonucleotide reductase small subunitUniRule annotation
Gene namesi
Name:RIR2UniRule annotation
OrganismiEquine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
Taxonomic identifieri10333 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]

Subcellular locationi

  • Host membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei164 – 184HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001905091 – 320Ribonucleoside-diphosphate reductase small subunitAdd BLAST320

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Protein-protein interaction databases

IntActiP50644. 2 interactors.
MINTiMINT-6732622.

Structurei

3D structure databases

ProteinModelPortaliP50644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase small chain family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

CDDicd01049. RNRR2. 1 hit.
Gene3Di1.10.620.20. 1 hit.
HAMAPiMF_04028. HSV_RIR2. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR033909. RNR_small.
IPR030475. RNR_small_AS.
IPR000358. RNR_small_fam.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALENSKKTD FADELLINAY FYTPECPDIE HLRLLSVANR WLDTDLPISD
60 70 80 90 100
DLKDVAKLAP AEREFYRFLF AFLSAADDLV NLNLGDLSAL FTQKDILHYY
110 120 130 140 150
IEQESIEVTH SRVYSAIQLM LFGNDATARA RYVASVVKDV AIDLKVSWLQ
160 170 180 190 200
AKVRECKSVA EKYILMILIE GVFFASSFAS IAYLRTHNLF VVTCQSNDLI
210 220 230 240 250
SRDEAIHTNA SCCIYNNYLG GFEKPAPTRI YALFSEAVNI ECEFLLSHAP
260 270 280 290 300
KSSHLLDIEA IICYVRYSAD RLLGEIGLSP LFNAPKPPPS FPLAFMTVEK
310 320
HTNFFERRST AYSGTLINDL
Length:320
Mass (Da):36,172
Last modified:October 1, 1996 - v1
Checksum:i7AF3511937730202
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75354 Genomic DNA. Translation: CAA53101.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75354 Genomic DNA. Translation: CAA53101.1.

3D structure databases

ProteinModelPortaliP50644.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP50644. 2 interactors.
MINTiMINT-6732622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

CDDicd01049. RNRR2. 1 hit.
Gene3Di1.10.620.20. 1 hit.
HAMAPiMF_04028. HSV_RIR2. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR033909. RNR_small.
IPR030475. RNR_small_AS.
IPR000358. RNR_small_fam.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR2_EHV4
AccessioniPrimary (citable) accession number: P50644
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.