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P50643

- RIR1_SUHVK

UniProt

P50643 - RIR1_SUHVK

Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RR1

Organism
Suid herpesvirus 1 (strain Kaplan) (SuHV-1) (Pseudorabies virus (strain Kaplan))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei262 – 2621SubstrateBy similarity
    Sitei278 – 2781Important for hydrogen atom transferBy similarity
    Binding sitei308 – 3081Substrate; via amide nitrogenBy similarity
    Active sitei489 – 4891Proton acceptorBy similarity
    Active sitei491 – 4911Cysteine radical intermediateBy similarity
    Active sitei493 – 4931Proton acceptorBy similarity
    Sitei506 – 5061Important for hydrogen atom transferBy similarity
    Sitei810 – 8101Important for electron transferBy similarity
    Sitei811 – 8111Important for electron transferBy similarity
    Sitei830 – 8301Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei833 – 8331Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase large subunit
    Gene namesi
    Name:RR1
    Synonyms:UL39
    OrganismiSuid herpesvirus 1 (strain Kaplan) (SuHV-1) (Pseudorabies virus (strain Kaplan))
    Taxonomic identifieri33703 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 835835Ribonucleoside-diphosphate reductase large subunitPRO_0000187244Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi278 ↔ 506Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP50643.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni277 – 2782Substrate bindingBy similarity
    Regioni489 – 4935Substrate bindingBy similarity
    Regioni666 – 6705Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view]
    PfamiPF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P50643-1 [UniParc]FASTAAdd to Basket

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    MPPRAPRPAG AVSPPFPPLA GPPLKARAPR ARDSPLTSPC RAHAAMASVV    50
    APAASSSAAA PGADAFLDAA CPEDVARALA AELEALRALG HDVGAPAPGA 100
    SRREAALFIT RAVDGLKAFS RVDERVYVAC GKLVHLRVRS READLDAWLA 150
    SPELALIPAV AAAVRRHRAR VEAALRWFWR EAYPALYARG LQSALKYEEM 200
    YLARLEHGRC EAMDQFFVRL AAAAATATRR PMALVLCGSD AWPEVFDAYF 250
    RALATQAIVP ATPLMLFAGR ARGSLASCYL LNPLPRTTEE AVRAITDEVA 300
    PILLRRGGVG LSLQSFNRTP SGDCTRGIMA VLKALDSMTA AINSDSERPT 350
    GVCVYVEPWH ADVRAVLNMR GMLAADESLR CDNIFSCLWT PDLFFQRYQR 400
    HLDGERAVKW TLFDDRASHL ASLHGPDFAR EYERLERLGL GVESLPIQDM 450
    AFLIVRSAVM TGSPFLMMKD ACNRHFHTDT RGAALATSNL CTEIVQRATP 500
    GENGVCNLAS VNLPACLAGG AFDFAALRRA ARVAAVFVNA MMRIGNYPTG 550
    ASVEGVRRSR SLGIGLQGLH TTVLALDMDM ADPAARRLNA AIAEELLYGV 600
    MDASVELCER GLRPFDGFEH SRYARGVMPF DAYERVSLRE PMRWDALRVR 650
    IAEHGVYNAQ FVALMPTVSS SQVTESSEGF SPTFTNMFSK VTISGELLRP 700
    NLPLMETLRR LFPRECARRD AVARLERAQW SVAAAFGELP AGHPLAKFKT 750
    AFEYDQELLI DMCADRAPFV DHSQSMSLFL TEPADGKLHA SRVMGLLMRA 800
    YNLGLKTGMY YCKIRKATNN GVFTGGDLVC TSCHL 835
    Length:835
    Mass (Da):91,060
    Last modified:October 1, 1996 - v1
    Checksum:i20D4AF7366667EBA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72087 Genomic DNA. Translation: CAA50976.1.
    X80797 Genomic DNA. Translation: CAA56775.1.
    PIRiS47345. S40140.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72087 Genomic DNA. Translation: CAA50976.1 .
    X80797 Genomic DNA. Translation: CAA56775.1 .
    PIRi S47345. S40140.

    3D structure databases

    ProteinModelPortali P50643.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    InterProi IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view ]
    Pfami PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Large and small subunits of the Aujeszky's disease virus ribonucleotide reductase: nucleotide sequence and putative structure."
      Kaliman A., Boldogkoi Z., Fodor I.
      Biochim. Biophys. Acta 1219:151-156(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Tinkering with a viral ribonucleotide reductase."
      Lembo D., Brune W.
      Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiRIR1_SUHVK
    AccessioniPrimary (citable) accession number: P50643
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3