P50643 (RIR1_SUHVK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase large subunit
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase large subunit

Gene names

Name:	RR1
Synonyms:	UL39

Organism

Suid herpesvirus 1 (strain Kaplan) (SuHV-1) (Pseudorabies virus (strain Kaplan))

Taxonomic identifier

33703 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus

Virus host

Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	835 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 835	835	Ribonucleoside-diphosphate reductase large subunit		PRO_0000187244
Regions
	Region	277 – 278	2	Substrate binding By similarity
	Region	489 – 493	5	Substrate binding By similarity
	Region	666 – 670	5	Substrate binding By similarity
Sites
	Active site	489	1	Proton acceptor By similarity
	Active site	491	1	Cysteine radical intermediate By similarity
	Active site	493	1	Proton acceptor By similarity
	Binding site	262	1	Substrate By similarity
	Binding site	308	1	Substrate; via amide nitrogen By similarity
	Site	278	1	Important for hydrogen atom transfer By similarity
	Site	506	1	Important for hydrogen atom transfer By similarity
	Site	810	1	Important for electron transfer By similarity
	Site	811	1	Important for electron transfer By similarity
	Site	830	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	833	1	Interacts with thioredoxin/glutaredoxin By similarity
Amino acid modifications
	Disulfide bond	278 ↔ 506		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50643 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 20D4AF7366667EBA	FASTA	835	91,060
	10 20 30 40 50 60 MPPRAPRPAG AVSPPFPPLA GPPLKARAPR ARDSPLTSPC RAHAAMASVV APAASSSAAA 70 80 90 100 110 120 PGADAFLDAA CPEDVARALA AELEALRALG HDVGAPAPGA SRREAALFIT RAVDGLKAFS 130 140 150 160 170 180 RVDERVYVAC GKLVHLRVRS READLDAWLA SPELALIPAV AAAVRRHRAR VEAALRWFWR 190 200 210 220 230 240 EAYPALYARG LQSALKYEEM YLARLEHGRC EAMDQFFVRL AAAAATATRR PMALVLCGSD 250 260 270 280 290 300 AWPEVFDAYF RALATQAIVP ATPLMLFAGR ARGSLASCYL LNPLPRTTEE AVRAITDEVA 310 320 330 340 350 360 PILLRRGGVG LSLQSFNRTP SGDCTRGIMA VLKALDSMTA AINSDSERPT GVCVYVEPWH 370 380 390 400 410 420 ADVRAVLNMR GMLAADESLR CDNIFSCLWT PDLFFQRYQR HLDGERAVKW TLFDDRASHL 430 440 450 460 470 480 ASLHGPDFAR EYERLERLGL GVESLPIQDM AFLIVRSAVM TGSPFLMMKD ACNRHFHTDT 490 500 510 520 530 540 RGAALATSNL CTEIVQRATP GENGVCNLAS VNLPACLAGG AFDFAALRRA ARVAAVFVNA 550 560 570 580 590 600 MMRIGNYPTG ASVEGVRRSR SLGIGLQGLH TTVLALDMDM ADPAARRLNA AIAEELLYGV 610 620 630 640 650 660 MDASVELCER GLRPFDGFEH SRYARGVMPF DAYERVSLRE PMRWDALRVR IAEHGVYNAQ 670 680 690 700 710 720 FVALMPTVSS SQVTESSEGF SPTFTNMFSK VTISGELLRP NLPLMETLRR LFPRECARRD 730 740 750 760 770 780 AVARLERAQW SVAAAFGELP AGHPLAKFKT AFEYDQELLI DMCADRAPFV DHSQSMSLFL 790 800 810 820 830 TEPADGKLHA SRVMGLLMRA YNLGLKTGMY YCKIRKATNN GVFTGGDLVC TSCHL

References

[1]	"Large and small subunits of the Aujeszky's disease virus ribonucleotide reductase: nucleotide sequence and putative structure." Kaliman A., Boldogkoi Z., Fodor I. Biochim. Biophys. Acta 1219:151-156(1994) [PubMed: 8086454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Tinkering with a viral ribonucleotide reductase." Lembo D., Brune W. Trends Biochem. Sci. 34:25-32(2009) [PubMed: 18990579] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72087 Genomic DNA. Translation: CAA50976.1. X80797 Genomic DNA. Translation: CAA56775.1.
PIR	S40140. S47345.
3D structure databases
ProteinModelPortal	P50643.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_SUHVK

Accession

Primary (citable) accession number: P50643

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 21, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program