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P50643

- RIR1_SUHVK

UniProt

P50643 - RIR1_SUHVK

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RR1

Organism
Suid herpesvirus 1 (strain Kaplan) (SuHV-1) (Pseudorabies virus (strain Kaplan))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei262 – 2621SubstrateBy similarity
Sitei278 – 2781Important for hydrogen atom transferBy similarity
Binding sitei308 – 3081Substrate; via amide nitrogenBy similarity
Active sitei489 – 4891Proton acceptorBy similarity
Active sitei491 – 4911Cysteine radical intermediateBy similarity
Active sitei493 – 4931Proton acceptorBy similarity
Sitei506 – 5061Important for hydrogen atom transferBy similarity
Sitei810 – 8101Important for electron transferBy similarity
Sitei811 – 8111Important for electron transferBy similarity
Sitei830 – 8301Interacts with thioredoxin/glutaredoxinBy similarity
Sitei833 – 8331Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Name:RR1
Synonyms:UL39
OrganismiSuid herpesvirus 1 (strain Kaplan) (SuHV-1) (Pseudorabies virus (strain Kaplan))
Taxonomic identifieri33703 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 835835Ribonucleoside-diphosphate reductase large subunitPRO_0000187244Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi278 ↔ 506Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP50643.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni277 – 2782Substrate bindingBy similarity
Regioni489 – 4935Substrate bindingBy similarity
Regioni666 – 6705Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50643 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPRAPRPAG AVSPPFPPLA GPPLKARAPR ARDSPLTSPC RAHAAMASVV
60 70 80 90 100
APAASSSAAA PGADAFLDAA CPEDVARALA AELEALRALG HDVGAPAPGA
110 120 130 140 150
SRREAALFIT RAVDGLKAFS RVDERVYVAC GKLVHLRVRS READLDAWLA
160 170 180 190 200
SPELALIPAV AAAVRRHRAR VEAALRWFWR EAYPALYARG LQSALKYEEM
210 220 230 240 250
YLARLEHGRC EAMDQFFVRL AAAAATATRR PMALVLCGSD AWPEVFDAYF
260 270 280 290 300
RALATQAIVP ATPLMLFAGR ARGSLASCYL LNPLPRTTEE AVRAITDEVA
310 320 330 340 350
PILLRRGGVG LSLQSFNRTP SGDCTRGIMA VLKALDSMTA AINSDSERPT
360 370 380 390 400
GVCVYVEPWH ADVRAVLNMR GMLAADESLR CDNIFSCLWT PDLFFQRYQR
410 420 430 440 450
HLDGERAVKW TLFDDRASHL ASLHGPDFAR EYERLERLGL GVESLPIQDM
460 470 480 490 500
AFLIVRSAVM TGSPFLMMKD ACNRHFHTDT RGAALATSNL CTEIVQRATP
510 520 530 540 550
GENGVCNLAS VNLPACLAGG AFDFAALRRA ARVAAVFVNA MMRIGNYPTG
560 570 580 590 600
ASVEGVRRSR SLGIGLQGLH TTVLALDMDM ADPAARRLNA AIAEELLYGV
610 620 630 640 650
MDASVELCER GLRPFDGFEH SRYARGVMPF DAYERVSLRE PMRWDALRVR
660 670 680 690 700
IAEHGVYNAQ FVALMPTVSS SQVTESSEGF SPTFTNMFSK VTISGELLRP
710 720 730 740 750
NLPLMETLRR LFPRECARRD AVARLERAQW SVAAAFGELP AGHPLAKFKT
760 770 780 790 800
AFEYDQELLI DMCADRAPFV DHSQSMSLFL TEPADGKLHA SRVMGLLMRA
810 820 830
YNLGLKTGMY YCKIRKATNN GVFTGGDLVC TSCHL
Length:835
Mass (Da):91,060
Last modified:October 1, 1996 - v1
Checksum:i20D4AF7366667EBA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72087 Genomic DNA. Translation: CAA50976.1.
X80797 Genomic DNA. Translation: CAA56775.1.
PIRiS47345. S40140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72087 Genomic DNA. Translation: CAA50976.1 .
X80797 Genomic DNA. Translation: CAA56775.1 .
PIRi S47345. S40140.

3D structure databases

ProteinModelPortali P50643.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Large and small subunits of the Aujeszky's disease virus ribonucleotide reductase: nucleotide sequence and putative structure."
    Kaliman A., Boldogkoi Z., Fodor I.
    Biochim. Biophys. Acta 1219:151-156(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_SUHVK
AccessioniPrimary (citable) accession number: P50643
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3