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P50642

- RIR1_EHV4

UniProt

P50642 - RIR1_EHV4

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene
N/A
Organism
Equine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei207 – 2071SubstrateBy similarity
Sitei223 – 2231Important for hydrogen atom transferBy similarity
Binding sitei253 – 2531Substrate; via amide nitrogenBy similarity
Active sitei435 – 4351Proton acceptorBy similarity
Active sitei437 – 4371Cysteine radical intermediateBy similarity
Active sitei439 – 4391Proton acceptorBy similarity
Sitei452 – 4521Important for hydrogen atom transferBy similarity
Sitei764 – 7641Important for electron transferBy similarity
Sitei765 – 7651Important for electron transferBy similarity
Sitei784 – 7841Interacts with thioredoxin/glutaredoxinBy similarity
Sitei787 – 7871Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
OrganismiEquine herpesvirus 4 (strain 1942) (EHV-4) (Equine rhinopneumonitis virus)
Taxonomic identifieri10333 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 789789Ribonucleoside-diphosphate reductase large subunitPRO_0000187242Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi223 ↔ 452Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Protein-protein interaction databases

IntActiP50642. 2 interactions.
MINTiMINT-6732615.

Structurei

3D structure databases

ProteinModelPortaliP50642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni222 – 2232Substrate bindingBy similarity
Regioni435 – 4395Substrate bindingBy similarity
Regioni620 – 6245Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50642-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALDFLSTDC PLGIVSDIIS NVNTIKEYGY SSELSTTLAP RPSREQVLEY
60 70 80 90 100
ITRVVDKLKP LCRVDERLYI ACGELVHLRI KARNTDLKYW LKSSEIDLSD
110 120 130 140 150
VVEQAILEHI DFVQKTLNSF ETSEYRDLCS LGLQSALKYE EMYLAKMRGG
160 170 180 190 200
RLESMGQFFL RLATTATHYT MEQPAMARVL VSGEVGWTYI FRAFFTALAG
210 220 230 240 250
QVVIPATPIM LFGGRDCGSM ASCYLLNPRV TDMNSAIPAL MEEVGPILCN
260 270 280 290 300
RGGIGLSLQR FNTPPTEGCS RGVMALLKLL DSMTMAINSD GERPTGVCVY
310 320 330 340 350
FEPWHADIRA ILNMRGMLAR DETVRCDNIF ACMWTPDLFF DRYQRYVDGE
360 370 380 390 400
SGIMWTLFDD TASHLCHMYG NDFTREYERL ERCGFGIDAI PIQDMAFIIV
410 420 430 440 450
RSAVMTGSPF LMFKDACNRH YHFDMRQRGA IMGSNLCTEI IQHADETQNG
460 470 480 490 500
VCNLASINLP KCLALPPPNI AGVPYFDFAA LGRAAATATI FVNAMMCAST
510 520 530 540 550
YPTVKSQKGV EENRSLGLGI QGLHTTFLML DLDMASPEAH QLNKQIAERL
560 570 580 590 600
LLNSMKASAT LCKLGMQPFK GFEDSKYSRG ELPFDAYPNV TLTNRNAWRR
610 620 630 640 650
LRTDIKQYGL YNSQFVAYMP TVSSSQVTES SEGFSPVYTN LFSKVTATGE
660 670 680 690 700
VLRPNVLLMR TIRSIFPQEC ARLQALSTLE AAKWSVVGAF GDLPVGHPLS
710 720 730 740 750
KFKTAFEYDQ TMLINMCADR AAFVDQSQSM SLFITEPADG KLPASRIMNL
760 770 780
LVHAYKRGLK TGMYYCKIKK ATNNGVFVGG DLVCTSCSL
Length:789
Mass (Da):88,047
Last modified:October 1, 1996 - v1
Checksum:i7CD9A24F3147988B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75354 Genomic DNA. Translation: CAA53100.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75354 Genomic DNA. Translation: CAA53100.1 .

3D structure databases

ProteinModelPortali P50642.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P50642. 2 interactions.
MINTi MINT-6732615.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequences of the ribonucleotide reductase-encoding genes of equine herpesvirus 4."
    Riggio M.P., Onions D.E.
    Gene 143:217-222(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_EHV4
AccessioniPrimary (citable) accession number: P50642
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3