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P50637

- TSPO_MOUSE

UniProt

P50637 - TSPO_MOUSE

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Protein

Translocator protein

Gene

Tspo

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides. Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:9832438, PubMed:24814875), but its precise physiological role is controversial. According to some reports, it is not required for steroid hormone biosynthesis (PubMed:24174323, PubMed:24936060).By similarity5 Publications

GO - Molecular functioni

  1. androgen binding Source: Ensembl
  2. benzodiazepine receptor activity Source: Ensembl

GO - Biological processi

  1. adrenal gland development Source: Ensembl
  2. aging Source: Ensembl
  3. behavioral response to pain Source: Ensembl
  4. cellular hypotonic response Source: Ensembl
  5. cellular response to lipopolysaccharide Source: Ensembl
  6. cellular response to zinc ion Source: Ensembl
  7. chloride transport Source: Ensembl
  8. contact inhibition Source: Ensembl
  9. glial cell migration Source: Ensembl
  10. lipid transport Source: UniProtKB-KW
  11. negative regulation of glial cell proliferation Source: Ensembl
  12. negative regulation of nitric oxide biosynthetic process Source: Ensembl
  13. negative regulation of tumor necrosis factor production Source: Ensembl
  14. peripheral nervous system axon regeneration Source: Ensembl
  15. positive regulation of apoptotic process Source: Ensembl
  16. positive regulation of calcium ion transport Source: Ensembl
  17. positive regulation of glial cell proliferation Source: Ensembl
  18. positive regulation of mitochondrial depolarization Source: Ensembl
  19. positive regulation of necrotic cell death Source: Ensembl
  20. positive regulation of reactive oxygen species metabolic process Source: Ensembl
  21. regulation of steroid biosynthetic process Source: Ensembl
  22. response to drug Source: Ensembl
  23. response to manganese ion Source: Ensembl
  24. response to progesterone Source: Ensembl
  25. response to testosterone Source: Ensembl
  26. response to vitamin B1 Source: Ensembl
  27. steroid biosynthetic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Lipid transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Translocator protein
Alternative name(s):
Mitochondrial benzodiazepine receptor
PKBS
Peripheral-type benzodiazepine receptor
Short name:
PBR
Gene namesi
Name:Tspo
Synonyms:Bzrp, Mbr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:88222. Tspo.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
  3. mitochondrial outer membrane Source: Ensembl
  4. mitochondrion Source: MGI
  5. nuclear membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype. Mice are viable and fertile and present only very minor changes in gonadal and adrenal steroid hormone production. testis-specific gene disruption (PubMed:24174323) does not affect testosterone production, gametogenesis and male fertility.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi153 – 1531Y → S: Abolishes cholesterol transport. 1 Publication
Mutagenesisi156 – 1561R → L: Abolishes cholesterol transport. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 169169Translocator proteinPRO_0000190998Add
BLAST

Proteomic databases

MaxQBiP50637.
PaxDbiP50637.
PRIDEiP50637.

Expressioni

Tissue specificityi

Detected in liver (at protein level). Ubiquitous.2 Publications

Inductioni

By dimethyl sulfoxide and diazepam.1 Publication

Gene expression databases

BgeeiP50637.
CleanExiMM_TSPO.
ExpressionAtlasiP50637. baseline and differential.
GenevestigatoriP50637.

Interactioni

Subunit structurei

Interacts with BZRAP1. Interacts with MOST-1. May interact with STAR.By similarity

Structurei

Secondary structure

1
169
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 3631Combined sources
Helixi46 – 494Combined sources
Helixi52 – 7120Combined sources
Beta strandi72 – 754Combined sources
Helixi76 – 9318Combined sources
Helixi96 – 983Combined sources
Turni99 – 1035Combined sources
Helixi105 – 1084Combined sources
Helixi109 – 12517Combined sources
Turni126 – 1294Combined sources
Helixi131 – 1377Combined sources
Helixi138 – 1403Combined sources
Helixi141 – 15818Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MGYNMR-A1-167[»]
ProteinModelPortaliP50637.
SMRiP50637. Positions 1-169.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55Mitochondrial intermembrane1 Publication
Topological domaini27 – 4620Cytoplasmic1 PublicationAdd
BLAST
Topological domaini68 – 7912Mitochondrial intermembrane1 PublicationAdd
BLAST
Topological domaini101 – 1055Cytoplasmic1 Publication
Topological domaini127 – 1348Mitochondrial intermembrane1 Publication
Topological domaini156 – 16914Cytoplasmic1 PublicationAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621Helical; Name=1Add
BLAST
Transmembranei47 – 6721Helical; Name=2Add
BLAST
Transmembranei80 – 10021Helical; Name=3Add
BLAST
Transmembranei106 – 12621Helical; Name=4Add
BLAST
Transmembranei135 – 15521Helical; Name=5Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the TspO/BZRP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3476.
HOGENOMiHOG000224659.
HOVERGENiHBG001296.
InParanoidiP50637.
KOiK05770.
OMAiALNYRIW.
OrthoDBiEOG7V7687.
PhylomeDBiP50637.
TreeFamiTF342852.

Family and domain databases

InterProiIPR004307. TspO_MBR.
[Graphical view]
PANTHERiPTHR10057. PTHR10057. 1 hit.
PfamiPF03073. TspO_MBR. 1 hit.
[Graphical view]
PIRSFiPIRSF005859. PBR. 1 hit.

Sequencei

Sequence statusi: Complete.

P50637-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPESWVPAVG LTLVPSLGGF MGAYFVRGEG LRWYASLQKP SWHPPRWTLA
60 70 80 90 100
PIWGTLYSAM GYGSYIVWKE LGGFTEDAMV PLGLYTGQLA LNWAWPPIFF
110 120 130 140 150
GARQMGWALA DLLLVSGVAT ATTLAWHRVS PPAARLLYPY LAWLAFATVL
160
NYYVWRDNSG RRGGSRLPE
Length:169
Mass (Da):18,841
Last modified:October 1, 1996 - v1
Checksum:i2EF93420C3842A85
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541G → A in BAA04749. (PubMed:8125973)Curated
Sequence conflicti168 – 1681P → A in BAA04749. (PubMed:8125973)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L17306 mRNA. Translation: AAA20127.1.
D21207 mRNA. Translation: BAA04749.1.
U12419 Genomic DNA. Translation: AAA83253.1.
CCDSiCCDS27705.1.
PIRiA53405.
I57953.
RefSeqiNP_033905.3. NM_009775.4.
UniGeneiMm.1508.

Genome annotation databases

EnsembliENSMUST00000047419; ENSMUSP00000037039; ENSMUSG00000041736.
GeneIDi12257.
KEGGimmu:12257.
UCSCiuc007xbg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L17306 mRNA. Translation: AAA20127.1 .
D21207 mRNA. Translation: BAA04749.1 .
U12419 Genomic DNA. Translation: AAA83253.1 .
CCDSi CCDS27705.1.
PIRi A53405.
I57953.
RefSeqi NP_033905.3. NM_009775.4.
UniGenei Mm.1508.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2MGY NMR - A 1-167 [» ]
ProteinModelPortali P50637.
SMRi P50637. Positions 1-169.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P50637.
ChEMBLi CHEMBL2149.

Proteomic databases

MaxQBi P50637.
PaxDbi P50637.
PRIDEi P50637.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047419 ; ENSMUSP00000037039 ; ENSMUSG00000041736 .
GeneIDi 12257.
KEGGi mmu:12257.
UCSCi uc007xbg.2. mouse.

Organism-specific databases

CTDi 706.
MGIi MGI:88222. Tspo.

Phylogenomic databases

eggNOGi COG3476.
HOGENOMi HOG000224659.
HOVERGENi HBG001296.
InParanoidi P50637.
KOi K05770.
OMAi ALNYRIW.
OrthoDBi EOG7V7687.
PhylomeDBi P50637.
TreeFami TF342852.

Miscellaneous databases

NextBioi 280682.
PROi P50637.
SOURCEi Search...

Gene expression databases

Bgeei P50637.
CleanExi MM_TSPO.
ExpressionAtlasi P50637. baseline and differential.
Genevestigatori P50637.

Family and domain databases

InterProi IPR004307. TspO_MBR.
[Graphical view ]
PANTHERi PTHR10057. PTHR10057. 1 hit.
Pfami PF03073. TspO_MBR. 1 hit.
[Graphical view ]
PIRSFi PIRSF005859. PBR. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "In vitro reconstitution of a functional peripheral-type benzodiazepine receptor from mouse Leydig tumor cells."
    Garnier M., Dimchev A.B., Boujrad N., Price J.M., Musto N.A., Papadopoulos V.
    Mol. Pharmacol. 45:201-211(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Testis.
  2. "Induction of peripheral-type benzodiazepine receptors during differentiation of mouse erythroleukemia cells. A possible involvement of these receptors in heme biosynthesis."
    Taketani S., Kohno H., Okuda M., Furukawa T., Tokunaga R.
    J. Biol. Chem. 269:7527-7531(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Strain: BALB/c.
    Tissue: Erythroleukemia.
  3. "Comparison of repetitive elements in the third intron of human and rodent mitochondrial benzodiazepine receptor-encoding genes."
    Yakovlev A.G., Ruffo M., Jurka J., Krueger K.E.
    Gene 155:201-205(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-125.
  4. "Peripheral-type benzodiazepine receptor function in cholesterol transport. Identification of a putative cholesterol recognition/interaction amino acid sequence and consensus pattern."
    Li H., Papadopoulos V.
    Endocrinology 139:4991-4997(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TYR-153 AND ARG-156.
  5. "Targeting mitochondrial 18kDa translocator protein (TSPO) regulates macrophage cholesterol efflux and lipid phenotype."
    Taylor J.M., Allen A.M., Graham A.
    Clin. Sci. 127:603-613(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Translocator protein/peripheral benzodiazepine receptor is not required for steroid hormone biosynthesis."
    Morohaku K., Pelton S.H., Daugherty D.J., Butler W.R., Deng W., Selvaraj V.
    Endocrinology 155:89-97(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  7. "Peripheral benzodiazepine receptor/translocator protein global knockout mice are viable with no effects on steroid hormone biosynthesis."
    Tu L.N., Morohaku K., Manna P.R., Pelton S.H., Butler W.R., Stocco D.M., Selvaraj V.
    J. Biol. Chem. 0:0-0(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  8. "Structure of the mitochondrial translocator protein in complex with a diagnostic ligand."
    Jaremko L., Jaremko M., Giller K., Becker S., Zweckstetter M.
    Science 343:1363-1366(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR IN COMPLEX WITH SYNTHETIC LIGAND PK11195, TOPOLOGY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTSPO_MOUSE
AccessioniPrimary (citable) accession number: P50637
Secondary accession number(s): Q62118, Q6LCZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3