P50637 (TSPO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translocator protein

Alternative name(s):
Mitochondrial benzodiazepine receptor
PKBS
Peripheral-type benzodiazepine receptor
Short name=PBR

Gene names

Name:	Tspo
Synonyms:	Bzrp, Mbr

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme By similarity. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells By similarity.
Subunit structure	Interacts with BZRAP1 By similarity. Interacts with MOST-1 By similarity. May interact with STAR By similarity.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein.
Induction	By dimethyl sulfoxide and diazepam. Ref.2
Sequence similarities	Belongs to the TspO/BZRP family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Molecular function	Receptor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adrenal gland development Inferred from electronic annotation. Source: Compara aging Inferred from electronic annotation. Source: Compara behavioral response to pain Inferred from electronic annotation. Source: Compara cellular hypotonic response Inferred from electronic annotation. Source: Compara cellular response to lipopolysaccharide Inferred from electronic annotation. Source: Compara cellular response to zinc ion Inferred from electronic annotation. Source: Compara contact inhibition Inferred from electronic annotation. Source: Compara glial cell migration Inferred from electronic annotation. Source: Compara negative regulation of glial cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara negative regulation of tumor necrosis factor production Inferred from electronic annotation. Source: Compara peripheral nervous system axon regeneration Inferred from electronic annotation. Source: Compara positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of calcium ion transport Inferred from electronic annotation. Source: Compara positive regulation of glial cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of mitochondrial depolarization Inferred from electronic annotation. Source: Compara positive regulation of necrotic cell death Inferred from electronic annotation. Source: Compara positive regulation of reactive oxygen species metabolic process Inferred from electronic annotation. Source: Compara regulation of steroid biosynthetic process Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to manganese ion Inferred from electronic annotation. Source: Compara response to progesterone stimulus Inferred from electronic annotation. Source: Compara response to testosterone stimulus Inferred from electronic annotation. Source: Compara response to vitamin B1 Inferred from electronic annotation. Source: Compara steroid biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: Compara mitochondrion Inferred from direct assay PubMed 14651853PubMed 18614015. Source: MGI
Molecular_function	androgen binding Inferred from electronic annotation. Source: Compara benzodiazepine receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Translocator protein		PRO_0000190998
Regions
	Transmembrane	6 – 26	21	Helical; Name=1; Potential
	Transmembrane	47 – 67	21	Helical; Name=2; Potential
	Transmembrane	80 – 100	21	Helical; Name=3; Potential
	Transmembrane	106 – 126	21	Helical; Name=4; Potential
	Transmembrane	135 – 155	21	Helical; Name=5; Potential
Experimental info
	Sequence conflict	54	1	G → A in BAA04749. Ref.2
	Sequence conflict	168	1	P → A in BAA04749. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P50637 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2EF93420C3842A85	FASTA	169	18,841
	10 20 30 40 50 60 MPESWVPAVG LTLVPSLGGF MGAYFVRGEG LRWYASLQKP SWHPPRWTLA PIWGTLYSAM 70 80 90 100 110 120 GYGSYIVWKE LGGFTEDAMV PLGLYTGQLA LNWAWPPIFF GARQMGWALA DLLLVSGVAT 130 140 150 160 ATTLAWHRVS PPAARLLYPY LAWLAFATVL NYYVWRDNSG RRGGSRLPE

References

[1]	"In vitro reconstitution of a functional peripheral-type benzodiazepine receptor from mouse Leydig tumor cells." Garnier M., Dimchev A.B., Boujrad N., Price J.M., Musto N.A., Papadopoulos V. Mol. Pharmacol. 45:201-211(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Induction of peripheral-type benzodiazepine receptors during differentiation of mouse erythroleukemia cells. A possible involvement of these receptors in heme biosynthesis." Taketani S., Kohno H., Okuda M., Furukawa T., Tokunaga R. J. Biol. Chem. 269:7527-7531(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: BALB/c. Tissue: Erythroleukemia.
[3]	"Comparison of repetitive elements in the third intron of human and rodent mitochondrial benzodiazepine receptor-encoding genes." Yakovlev A.G., Ruffo M., Jurka J., Krueger K.E. Gene 155:201-205(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-125.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L17306 mRNA. Translation: AAA20127.1. D21207 mRNA. Translation: BAA04749.1. U12419 Genomic DNA. Translation: AAA83253.1.
IPI	IPI00120283.
PIR	A53405. I57953.
RefSeq	NP_033905.3. NM_009775.4.
UniGene	Mm.1508.
3D structure databases
ModBase	Search...
Proteomic databases
PaxDb	P50637.
PRIDE	P50637.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000047419; ENSMUSP00000037039; ENSMUSG00000041736.
GeneID	12257.
KEGG	mmu:12257.
Organism-specific databases
CTD	706.
MGI	MGI:88222. Tspo.
Phylogenomic databases
eggNOG	COG3476.
HOGENOM	HOG000224659.
HOVERGEN	HBG001296.
InParanoid	P50637.
KO	K05770.
OMA	ARQMGWA.
OrthoDB	EOG4HMJBC.
Gene expression databases
ArrayExpress	P50637.
Bgee	P50637.
CleanEx	MM_TSPO.
Genevestigator	P50637.
GermOnline	ENSMUSG00000041736. Mus musculus.
Family and domain databases
InterPro	IPR004307. TspO_MBR. [Graphical view]
PANTHER	PTHR10057. PTHR10057. 1 hit.
Pfam	PF03073. TspO_MBR. 1 hit. [Graphical view]
PIRSF	PIRSF005859. PBR. 1 hit.
ProtoNet	Search...
Other
BindingDB	P50637.
ChEMBL	CHEMBL2149.
NextBio	280682.
SOURCE	Search...

Entry information

Entry name

TSPO_MOUSE

Accession

Primary (citable) accession number: P50637
Secondary accession number(s): Q62118, Q6LCZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program