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P50637

- TSPO_MOUSE

UniProt

P50637 - TSPO_MOUSE

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Protein
Translocator protein
Gene
Tspo, Bzrp, Mbr
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme By similarity. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides. Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (1 Publication, 1 Publication), but its precise physiological role is controversial. According to some reports, it is not required for steroid hormone biosynthesis (1 Publication, 1 Publication).

GO - Molecular functioni

  1. androgen binding Source: Ensembl
  2. benzodiazepine receptor activity Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. adrenal gland development Source: Ensembl
  2. aging Source: Ensembl
  3. behavioral response to pain Source: Ensembl
  4. cellular hypotonic response Source: Ensembl
  5. cellular response to lipopolysaccharide Source: Ensembl
  6. cellular response to zinc ion Source: Ensembl
  7. chloride transport Source: Ensembl
  8. contact inhibition Source: Ensembl
  9. glial cell migration Source: Ensembl
  10. negative regulation of glial cell proliferation Source: Ensembl
  11. negative regulation of nitric oxide biosynthetic process Source: Ensembl
  12. negative regulation of tumor necrosis factor production Source: Ensembl
  13. peripheral nervous system axon regeneration Source: Ensembl
  14. positive regulation of apoptotic process Source: Ensembl
  15. positive regulation of calcium ion transport Source: Ensembl
  16. positive regulation of glial cell proliferation Source: Ensembl
  17. positive regulation of mitochondrial depolarization Source: Ensembl
  18. positive regulation of necrotic cell death Source: Ensembl
  19. positive regulation of reactive oxygen species metabolic process Source: Ensembl
  20. regulation of steroid biosynthetic process Source: Ensembl
  21. response to drug Source: Ensembl
  22. response to manganese ion Source: Ensembl
  23. response to progesterone Source: Ensembl
  24. response to testosterone Source: Ensembl
  25. response to vitamin B1 Source: Ensembl
  26. steroid biosynthetic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Lipid transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Translocator protein
Alternative name(s):
Mitochondrial benzodiazepine receptor
PKBS
Peripheral-type benzodiazepine receptor
Short name:
PBR
Gene namesi
Name:Tspo
Synonyms:Bzrp, Mbr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:88222. Tspo.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55Mitochondrial intermembrane
Transmembranei6 – 2621Helical; Name=1
Add
BLAST
Topological domaini27 – 4620Cytoplasmic
Add
BLAST
Transmembranei47 – 6721Helical; Name=2
Add
BLAST
Topological domaini68 – 7912Mitochondrial intermembrane
Add
BLAST
Transmembranei80 – 10021Helical; Name=3
Add
BLAST
Topological domaini101 – 1055Cytoplasmic
Transmembranei106 – 12621Helical; Name=4
Add
BLAST
Topological domaini127 – 1348Mitochondrial intermembrane
Transmembranei135 – 15521Helical; Name=5
Add
BLAST
Topological domaini156 – 16914Cytoplasmic
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial outer membrane Source: Ensembl
  3. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype. Mice are viable and fertile and present only very minor changes in gonadal and adrenal steroid hormone production. testis-specific gene disruption (1 Publication) does not affect testosterone production, gametogenesis and male fertility.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi153 – 1531Y → S: Abolishes cholesterol transport.
Mutagenesisi156 – 1561R → L: Abolishes cholesterol transport.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 169169Translocator protein
PRO_0000190998Add
BLAST

Proteomic databases

PaxDbiP50637.
PRIDEiP50637.

Expressioni

Tissue specificityi

Detected in liver (at protein level). Ubiquitous.

Inductioni

By dimethyl sulfoxide and diazepam.1 Publication

Gene expression databases

ArrayExpressiP50637.
BgeeiP50637.
CleanExiMM_TSPO.
GenevestigatoriP50637.

Interactioni

Subunit structurei

Interacts with BZRAP1 By similarity. Interacts with MOST-1 By similarity. May interact with STAR By similarity.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 3631
Helixi46 – 494
Helixi52 – 7120
Beta strandi72 – 754
Helixi76 – 9318
Helixi96 – 983
Turni99 – 1035
Helixi105 – 1084
Helixi109 – 12517
Turni126 – 1294
Helixi131 – 1377
Helixi138 – 1403
Helixi141 – 15818

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MGYNMR-A1-167[»]
ProteinModelPortaliP50637.
SMRiP50637. Positions 1-169.

Family & Domainsi

Sequence similaritiesi

Belongs to the TspO/BZRP family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3476.
HOGENOMiHOG000224659.
HOVERGENiHBG001296.
InParanoidiP50637.
KOiK05770.
OMAiALNYRIW.
OrthoDBiEOG7V7687.
PhylomeDBiP50637.
TreeFamiTF342852.

Family and domain databases

InterProiIPR004307. TspO_MBR.
[Graphical view]
PANTHERiPTHR10057. PTHR10057. 1 hit.
PfamiPF03073. TspO_MBR. 1 hit.
[Graphical view]
PIRSFiPIRSF005859. PBR. 1 hit.

Sequencei

Sequence statusi: Complete.

P50637-1 [UniParc]FASTAAdd to Basket

« Hide

MPESWVPAVG LTLVPSLGGF MGAYFVRGEG LRWYASLQKP SWHPPRWTLA    50
PIWGTLYSAM GYGSYIVWKE LGGFTEDAMV PLGLYTGQLA LNWAWPPIFF 100
GARQMGWALA DLLLVSGVAT ATTLAWHRVS PPAARLLYPY LAWLAFATVL 150
NYYVWRDNSG RRGGSRLPE 169
Length:169
Mass (Da):18,841
Last modified:October 1, 1996 - v1
Checksum:i2EF93420C3842A85
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541G → A in BAA04749. 1 Publication
Sequence conflicti168 – 1681P → A in BAA04749. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L17306 mRNA. Translation: AAA20127.1.
D21207 mRNA. Translation: BAA04749.1.
U12419 Genomic DNA. Translation: AAA83253.1.
CCDSiCCDS27705.1.
PIRiA53405.
I57953.
RefSeqiNP_033905.3. NM_009775.4.
UniGeneiMm.1508.

Genome annotation databases

EnsembliENSMUST00000047419; ENSMUSP00000037039; ENSMUSG00000041736.
GeneIDi12257.
KEGGimmu:12257.
UCSCiuc007xbg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L17306 mRNA. Translation: AAA20127.1 .
D21207 mRNA. Translation: BAA04749.1 .
U12419 Genomic DNA. Translation: AAA83253.1 .
CCDSi CCDS27705.1.
PIRi A53405.
I57953.
RefSeqi NP_033905.3. NM_009775.4.
UniGenei Mm.1508.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2MGY NMR - A 1-167 [» ]
ProteinModelPortali P50637.
SMRi P50637. Positions 1-169.
ModBasei Search...

Chemistry

BindingDBi P50637.
ChEMBLi CHEMBL2149.

Proteomic databases

PaxDbi P50637.
PRIDEi P50637.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047419 ; ENSMUSP00000037039 ; ENSMUSG00000041736 .
GeneIDi 12257.
KEGGi mmu:12257.
UCSCi uc007xbg.2. mouse.

Organism-specific databases

CTDi 706.
MGIi MGI:88222. Tspo.

Phylogenomic databases

eggNOGi COG3476.
HOGENOMi HOG000224659.
HOVERGENi HBG001296.
InParanoidi P50637.
KOi K05770.
OMAi ALNYRIW.
OrthoDBi EOG7V7687.
PhylomeDBi P50637.
TreeFami TF342852.

Miscellaneous databases

NextBioi 280682.
PROi P50637.
SOURCEi Search...

Gene expression databases

ArrayExpressi P50637.
Bgeei P50637.
CleanExi MM_TSPO.
Genevestigatori P50637.

Family and domain databases

InterProi IPR004307. TspO_MBR.
[Graphical view ]
PANTHERi PTHR10057. PTHR10057. 1 hit.
Pfami PF03073. TspO_MBR. 1 hit.
[Graphical view ]
PIRSFi PIRSF005859. PBR. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "In vitro reconstitution of a functional peripheral-type benzodiazepine receptor from mouse Leydig tumor cells."
    Garnier M., Dimchev A.B., Boujrad N., Price J.M., Musto N.A., Papadopoulos V.
    Mol. Pharmacol. 45:201-211(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Testis.
  2. "Induction of peripheral-type benzodiazepine receptors during differentiation of mouse erythroleukemia cells. A possible involvement of these receptors in heme biosynthesis."
    Taketani S., Kohno H., Okuda M., Furukawa T., Tokunaga R.
    J. Biol. Chem. 269:7527-7531(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Strain: BALB/c.
    Tissue: Erythroleukemia.
  3. "Comparison of repetitive elements in the third intron of human and rodent mitochondrial benzodiazepine receptor-encoding genes."
    Yakovlev A.G., Ruffo M., Jurka J., Krueger K.E.
    Gene 155:201-205(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-125.
  4. "Peripheral-type benzodiazepine receptor function in cholesterol transport. Identification of a putative cholesterol recognition/interaction amino acid sequence and consensus pattern."
    Li H., Papadopoulos V.
    Endocrinology 139:4991-4997(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TYR-153 AND ARG-156.
  5. "Targeting mitochondrial 18kDa translocator protein (TSPO) regulates macrophage cholesterol efflux and lipid phenotype."
    Taylor J.M., Allen A.M., Graham A.
    Clin. Sci. 0:0-0(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Translocator protein/peripheral benzodiazepine receptor is not required for steroid hormone biosynthesis."
    Morohaku K., Pelton S.H., Daugherty D.J., Butler W.R., Deng W., Selvaraj V.
    Endocrinology 155:89-97(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  7. "Peripheral benzodiazepine receptor/translocator protein global knockout mice are viable with no effects on steroid hormone biosynthesis."
    Tu L.N., Morohaku K., Manna P.R., Pelton S.H., Butler W.R., Stocco D.M., Selvaraj V.
    J. Biol. Chem. 0:0-0(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  8. "Structure of the mitochondrial translocator protein in complex with a diagnostic ligand."
    Jaremko L., Jaremko M., Giller K., Becker S., Zweckstetter M.
    Science 343:1363-1366(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR IN COMPLEX WITH SYNTHETIC LIGAND PK11195, TOPOLOGY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTSPO_MOUSE
AccessioniPrimary (citable) accession number: P50637
Secondary accession number(s): Q62118, Q6LCZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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