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P50637

- TSPO_MOUSE

UniProt

P50637 - TSPO_MOUSE

Protein

Translocator protein

Gene

Tspo

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme By similarity. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides. Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:9832438, PubMed:24814875), but its precise physiological role is controversial. According to some reports, it is not required for steroid hormone biosynthesis (PubMed:24174323, PubMed:24936060).By similarity5 Publications

    GO - Molecular functioni

    1. androgen binding Source: Ensembl
    2. benzodiazepine receptor activity Source: Ensembl

    GO - Biological processi

    1. adrenal gland development Source: Ensembl
    2. aging Source: Ensembl
    3. behavioral response to pain Source: Ensembl
    4. cellular hypotonic response Source: Ensembl
    5. cellular response to lipopolysaccharide Source: Ensembl
    6. cellular response to zinc ion Source: Ensembl
    7. chloride transport Source: Ensembl
    8. contact inhibition Source: Ensembl
    9. glial cell migration Source: Ensembl
    10. negative regulation of glial cell proliferation Source: Ensembl
    11. negative regulation of nitric oxide biosynthetic process Source: Ensembl
    12. negative regulation of tumor necrosis factor production Source: Ensembl
    13. peripheral nervous system axon regeneration Source: Ensembl
    14. positive regulation of apoptotic process Source: Ensembl
    15. positive regulation of calcium ion transport Source: Ensembl
    16. positive regulation of glial cell proliferation Source: Ensembl
    17. positive regulation of mitochondrial depolarization Source: Ensembl
    18. positive regulation of necrotic cell death Source: Ensembl
    19. positive regulation of reactive oxygen species metabolic process Source: Ensembl
    20. regulation of steroid biosynthetic process Source: Ensembl
    21. response to drug Source: Ensembl
    22. response to manganese ion Source: Ensembl
    23. response to progesterone Source: Ensembl
    24. response to testosterone Source: Ensembl
    25. response to vitamin B1 Source: Ensembl
    26. steroid biosynthetic process Source: Ensembl

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Lipid transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Translocator protein
    Alternative name(s):
    Mitochondrial benzodiazepine receptor
    PKBS
    Peripheral-type benzodiazepine receptor
    Short name:
    PBR
    Gene namesi
    Name:Tspo
    Synonyms:Bzrp, Mbr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:88222. Tspo.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial outer membrane Source: Ensembl
    3. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    No obvious phenotype. Mice are viable and fertile and present only very minor changes in gonadal and adrenal steroid hormone production. testis-specific gene disruption (PubMed:24174323) does not affect testosterone production, gametogenesis and male fertility.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi153 – 1531Y → S: Abolishes cholesterol transport. 1 Publication
    Mutagenesisi156 – 1561R → L: Abolishes cholesterol transport. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 169169Translocator proteinPRO_0000190998Add
    BLAST

    Proteomic databases

    PaxDbiP50637.
    PRIDEiP50637.

    Expressioni

    Tissue specificityi

    Detected in liver (at protein level). Ubiquitous.2 Publications

    Inductioni

    By dimethyl sulfoxide and diazepam.1 Publication

    Gene expression databases

    ArrayExpressiP50637.
    BgeeiP50637.
    CleanExiMM_TSPO.
    GenevestigatoriP50637.

    Interactioni

    Subunit structurei

    Interacts with BZRAP1. Interacts with MOST-1. May interact with STAR.By similarity

    Structurei

    Secondary structure

    1
    169
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 3631
    Helixi46 – 494
    Helixi52 – 7120
    Beta strandi72 – 754
    Helixi76 – 9318
    Helixi96 – 983
    Turni99 – 1035
    Helixi105 – 1084
    Helixi109 – 12517
    Turni126 – 1294
    Helixi131 – 1377
    Helixi138 – 1403
    Helixi141 – 15818

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2MGYNMR-A1-167[»]
    ProteinModelPortaliP50637.
    SMRiP50637. Positions 1-169.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 55Mitochondrial intermembrane1 Publication
    Topological domaini27 – 4620Cytoplasmic1 PublicationAdd
    BLAST
    Topological domaini68 – 7912Mitochondrial intermembrane1 PublicationAdd
    BLAST
    Topological domaini101 – 1055Cytoplasmic1 Publication
    Topological domaini127 – 1348Mitochondrial intermembrane1 Publication
    Topological domaini156 – 16914Cytoplasmic1 PublicationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621Helical; Name=1Add
    BLAST
    Transmembranei47 – 6721Helical; Name=2Add
    BLAST
    Transmembranei80 – 10021Helical; Name=3Add
    BLAST
    Transmembranei106 – 12621Helical; Name=4Add
    BLAST
    Transmembranei135 – 15521Helical; Name=5Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TspO/BZRP family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3476.
    HOGENOMiHOG000224659.
    HOVERGENiHBG001296.
    InParanoidiP50637.
    KOiK05770.
    OMAiALNYRIW.
    OrthoDBiEOG7V7687.
    PhylomeDBiP50637.
    TreeFamiTF342852.

    Family and domain databases

    InterProiIPR004307. TspO_MBR.
    [Graphical view]
    PANTHERiPTHR10057. PTHR10057. 1 hit.
    PfamiPF03073. TspO_MBR. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005859. PBR. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P50637-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPESWVPAVG LTLVPSLGGF MGAYFVRGEG LRWYASLQKP SWHPPRWTLA    50
    PIWGTLYSAM GYGSYIVWKE LGGFTEDAMV PLGLYTGQLA LNWAWPPIFF 100
    GARQMGWALA DLLLVSGVAT ATTLAWHRVS PPAARLLYPY LAWLAFATVL 150
    NYYVWRDNSG RRGGSRLPE 169
    Length:169
    Mass (Da):18,841
    Last modified:October 1, 1996 - v1
    Checksum:i2EF93420C3842A85
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti54 – 541G → A in BAA04749. (PubMed:8125973)Curated
    Sequence conflicti168 – 1681P → A in BAA04749. (PubMed:8125973)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L17306 mRNA. Translation: AAA20127.1.
    D21207 mRNA. Translation: BAA04749.1.
    U12419 Genomic DNA. Translation: AAA83253.1.
    CCDSiCCDS27705.1.
    PIRiA53405.
    I57953.
    RefSeqiNP_033905.3. NM_009775.4.
    UniGeneiMm.1508.

    Genome annotation databases

    EnsembliENSMUST00000047419; ENSMUSP00000037039; ENSMUSG00000041736.
    GeneIDi12257.
    KEGGimmu:12257.
    UCSCiuc007xbg.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L17306 mRNA. Translation: AAA20127.1 .
    D21207 mRNA. Translation: BAA04749.1 .
    U12419 Genomic DNA. Translation: AAA83253.1 .
    CCDSi CCDS27705.1.
    PIRi A53405.
    I57953.
    RefSeqi NP_033905.3. NM_009775.4.
    UniGenei Mm.1508.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2MGY NMR - A 1-167 [» ]
    ProteinModelPortali P50637.
    SMRi P50637. Positions 1-169.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P50637.
    ChEMBLi CHEMBL2149.

    Proteomic databases

    PaxDbi P50637.
    PRIDEi P50637.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047419 ; ENSMUSP00000037039 ; ENSMUSG00000041736 .
    GeneIDi 12257.
    KEGGi mmu:12257.
    UCSCi uc007xbg.2. mouse.

    Organism-specific databases

    CTDi 706.
    MGIi MGI:88222. Tspo.

    Phylogenomic databases

    eggNOGi COG3476.
    HOGENOMi HOG000224659.
    HOVERGENi HBG001296.
    InParanoidi P50637.
    KOi K05770.
    OMAi ALNYRIW.
    OrthoDBi EOG7V7687.
    PhylomeDBi P50637.
    TreeFami TF342852.

    Miscellaneous databases

    NextBioi 280682.
    PROi P50637.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P50637.
    Bgeei P50637.
    CleanExi MM_TSPO.
    Genevestigatori P50637.

    Family and domain databases

    InterProi IPR004307. TspO_MBR.
    [Graphical view ]
    PANTHERi PTHR10057. PTHR10057. 1 hit.
    Pfami PF03073. TspO_MBR. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005859. PBR. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "In vitro reconstitution of a functional peripheral-type benzodiazepine receptor from mouse Leydig tumor cells."
      Garnier M., Dimchev A.B., Boujrad N., Price J.M., Musto N.A., Papadopoulos V.
      Mol. Pharmacol. 45:201-211(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Tissue: Testis.
    2. "Induction of peripheral-type benzodiazepine receptors during differentiation of mouse erythroleukemia cells. A possible involvement of these receptors in heme biosynthesis."
      Taketani S., Kohno H., Okuda M., Furukawa T., Tokunaga R.
      J. Biol. Chem. 269:7527-7531(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
      Strain: BALB/c.
      Tissue: Erythroleukemia.
    3. "Comparison of repetitive elements in the third intron of human and rodent mitochondrial benzodiazepine receptor-encoding genes."
      Yakovlev A.G., Ruffo M., Jurka J., Krueger K.E.
      Gene 155:201-205(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-125.
    4. "Peripheral-type benzodiazepine receptor function in cholesterol transport. Identification of a putative cholesterol recognition/interaction amino acid sequence and consensus pattern."
      Li H., Papadopoulos V.
      Endocrinology 139:4991-4997(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF TYR-153 AND ARG-156.
    5. "Targeting mitochondrial 18kDa translocator protein (TSPO) regulates macrophage cholesterol efflux and lipid phenotype."
      Taylor J.M., Allen A.M., Graham A.
      Clin. Sci. 0:0-0(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Translocator protein/peripheral benzodiazepine receptor is not required for steroid hormone biosynthesis."
      Morohaku K., Pelton S.H., Daugherty D.J., Butler W.R., Deng W., Selvaraj V.
      Endocrinology 155:89-97(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    7. "Peripheral benzodiazepine receptor/translocator protein global knockout mice are viable with no effects on steroid hormone biosynthesis."
      Tu L.N., Morohaku K., Manna P.R., Pelton S.H., Butler W.R., Stocco D.M., Selvaraj V.
      J. Biol. Chem. 0:0-0(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    8. "Structure of the mitochondrial translocator protein in complex with a diagnostic ligand."
      Jaremko L., Jaremko M., Giller K., Becker S., Zweckstetter M.
      Science 343:1363-1366(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR IN COMPLEX WITH SYNTHETIC LIGAND PK11195, TOPOLOGY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiTSPO_MOUSE
    AccessioniPrimary (citable) accession number: P50637
    Secondary accession number(s): Q62118, Q6LCZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3