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Protein

Translocator protein

Gene

Tspo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides. Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:9832438, PubMed:24814875), but its precise physiological role is controversial. According to some reports, it is not required for steroid hormone biosynthesis (PubMed:24174323, PubMed:24936060).By similarity5 Publications

GO - Molecular functioni

  • androgen binding Source: Ensembl
  • benzodiazepine receptor activity Source: Ensembl
  • ion channel binding Source: ParkinsonsUK-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Lipid transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Translocator protein
Alternative name(s):
Mitochondrial benzodiazepine receptor
PKBS
Peripheral-type benzodiazepine receptor
Short name:
PBR
Gene namesi
Name:Tspo
Synonyms:Bzrp, Mbr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:88222. Tspo.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 5Mitochondrial intermembrane1 Publication4
Transmembranei6 – 26Helical; Name=1Add BLAST21
Topological domaini27 – 46Cytoplasmic1 PublicationAdd BLAST20
Transmembranei47 – 67Helical; Name=2Add BLAST21
Topological domaini68 – 79Mitochondrial intermembrane1 PublicationAdd BLAST12
Transmembranei80 – 100Helical; Name=3Add BLAST21
Topological domaini101 – 105Cytoplasmic1 Publication5
Transmembranei106 – 126Helical; Name=4Add BLAST21
Topological domaini127 – 134Mitochondrial intermembrane1 Publication8
Transmembranei135 – 155Helical; Name=5Add BLAST21
Topological domaini156 – 169Cytoplasmic1 PublicationAdd BLAST14

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

No obvious phenotype. Mice are viable and fertile and present only very minor changes in gonadal and adrenal steroid hormone production. testis-specific gene disruption (PubMed:24174323) does not affect testosterone production, gametogenesis and male fertility.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi153Y → S: Abolishes cholesterol transport. 1 Publication1
Mutagenesisi156R → L: Abolishes cholesterol transport. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2149.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001909982 – 169Translocator proteinAdd BLAST168

Proteomic databases

PaxDbiP50637.
PeptideAtlasiP50637.
PRIDEiP50637.

PTM databases

iPTMnetiP50637.
PhosphoSitePlusiP50637.

Expressioni

Tissue specificityi

Detected in liver (at protein level). Ubiquitous.2 Publications

Inductioni

By dimethyl sulfoxide and diazepam.1 Publication

Gene expression databases

BgeeiENSMUSG00000041736.
CleanExiMM_TSPO.
ExpressionAtlasiP50637. baseline and differential.
GenevisibleiP50637. MM.

Interactioni

Subunit structurei

Interacts with TSPOAP1. Interacts with MOST-1. May interact with STAR.By similarity

GO - Molecular functioni

  • ion channel binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi198410. 1 interactor.
STRINGi10090.ENSMUSP00000037039.

Chemistry databases

BindingDBiP50637.

Structurei

Secondary structure

1169
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 36Combined sources31
Helixi46 – 49Combined sources4
Helixi52 – 71Combined sources20
Beta strandi72 – 75Combined sources4
Helixi76 – 93Combined sources18
Helixi96 – 98Combined sources3
Turni99 – 103Combined sources5
Helixi105 – 108Combined sources4
Helixi109 – 125Combined sources17
Turni126 – 129Combined sources4
Helixi131 – 137Combined sources7
Helixi138 – 140Combined sources3
Helixi141 – 158Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MGYNMR-A1-167[»]
2N02NMR-A1-169[»]
ProteinModelPortaliP50637.
SMRiP50637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TspO/BZRP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3797. Eukaryota.
COG3476. LUCA.
GeneTreeiENSGT00390000012980.
HOGENOMiHOG000224659.
HOVERGENiHBG001296.
InParanoidiP50637.
KOiK05770.
OMAiNPPDWLF.
OrthoDBiEOG091G0W79.
PhylomeDBiP50637.
TreeFamiTF342852.

Family and domain databases

InterProiIPR030164. TSPO.
IPR004307. TspO_MBR.
[Graphical view]
PANTHERiPTHR10057. PTHR10057. 1 hit.
PTHR10057:SF5. PTHR10057:SF5. 1 hit.
PfamiPF03073. TspO_MBR. 1 hit.
[Graphical view]
PIRSFiPIRSF005859. PBR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPESWVPAVG LTLVPSLGGF MGAYFVRGEG LRWYASLQKP SWHPPRWTLA
60 70 80 90 100
PIWGTLYSAM GYGSYIVWKE LGGFTEDAMV PLGLYTGQLA LNWAWPPIFF
110 120 130 140 150
GARQMGWALA DLLLVSGVAT ATTLAWHRVS PPAARLLYPY LAWLAFATVL
160
NYYVWRDNSG RRGGSRLPE
Length:169
Mass (Da):18,841
Last modified:October 1, 1996 - v1
Checksum:i2EF93420C3842A85
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54G → A in BAA04749 (PubMed:8125973).Curated1
Sequence conflicti168P → A in BAA04749 (PubMed:8125973).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17306 mRNA. Translation: AAA20127.1.
D21207 mRNA. Translation: BAA04749.1.
U12419 Genomic DNA. Translation: AAA83253.1.
CCDSiCCDS27705.1.
PIRiA53405.
I57953.
RefSeqiNP_033905.3. NM_009775.4.
UniGeneiMm.1508.

Genome annotation databases

EnsembliENSMUST00000047419; ENSMUSP00000037039; ENSMUSG00000041736.
GeneIDi12257.
KEGGimmu:12257.
UCSCiuc007xbg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17306 mRNA. Translation: AAA20127.1.
D21207 mRNA. Translation: BAA04749.1.
U12419 Genomic DNA. Translation: AAA83253.1.
CCDSiCCDS27705.1.
PIRiA53405.
I57953.
RefSeqiNP_033905.3. NM_009775.4.
UniGeneiMm.1508.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MGYNMR-A1-167[»]
2N02NMR-A1-169[»]
ProteinModelPortaliP50637.
SMRiP50637.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198410. 1 interactor.
STRINGi10090.ENSMUSP00000037039.

Chemistry databases

BindingDBiP50637.
ChEMBLiCHEMBL2149.

PTM databases

iPTMnetiP50637.
PhosphoSitePlusiP50637.

Proteomic databases

PaxDbiP50637.
PeptideAtlasiP50637.
PRIDEiP50637.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047419; ENSMUSP00000037039; ENSMUSG00000041736.
GeneIDi12257.
KEGGimmu:12257.
UCSCiuc007xbg.2. mouse.

Organism-specific databases

CTDi706.
MGIiMGI:88222. Tspo.

Phylogenomic databases

eggNOGiKOG3797. Eukaryota.
COG3476. LUCA.
GeneTreeiENSGT00390000012980.
HOGENOMiHOG000224659.
HOVERGENiHBG001296.
InParanoidiP50637.
KOiK05770.
OMAiNPPDWLF.
OrthoDBiEOG091G0W79.
PhylomeDBiP50637.
TreeFamiTF342852.

Miscellaneous databases

PROiP50637.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041736.
CleanExiMM_TSPO.
ExpressionAtlasiP50637. baseline and differential.
GenevisibleiP50637. MM.

Family and domain databases

InterProiIPR030164. TSPO.
IPR004307. TspO_MBR.
[Graphical view]
PANTHERiPTHR10057. PTHR10057. 1 hit.
PTHR10057:SF5. PTHR10057:SF5. 1 hit.
PfamiPF03073. TspO_MBR. 1 hit.
[Graphical view]
PIRSFiPIRSF005859. PBR. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTSPO_MOUSE
AccessioniPrimary (citable) accession number: P50637
Secondary accession number(s): Q62118, Q6LCZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.