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P50635

- APY_AEDAE

UniProt

P50635 - APY_AEDAE

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Protein

Apyrase

Gene

APY

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Facilitates hematophagy by preventing ADP-dependent platelet aggregation in the host. May reduce probing time by facilitating the speed of locating blood.

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Divalent cations.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Divalent metal cation 1By similarity
Metal bindingi49 – 491Divalent metal cation 1By similarity
Metal bindingi98 – 981Divalent metal cation 1By similarity
Metal bindingi98 – 981Divalent metal cation 2By similarity
Metal bindingi130 – 1301Divalent metal cation 2By similarity
Sitei131 – 1311Transition state stabilizerBy similarity
Sitei134 – 1341Transition state stabilizerBy similarity
Metal bindingi233 – 2331Divalent metal cation 2By similarity
Metal bindingi257 – 2571Divalent metal cation 2By similarity
Binding sitei424 – 4241SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity, acting on ester bonds Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. nucleotide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apyrase (EC:3.6.1.5)
Alternative name(s):
ATP-diphosphatase
Short name:
ADPase
ATP-diphosphohydrolase
Adenosine diphosphatase
Allergen: Aed a 1
Gene namesi
Name:APY
ORF Names:AAEL006347
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
ProteomesiUP000008820: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3. Aed a 1.
3539. Aed a 1.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 562538ApyrasePRO_0000000039Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Salivary gland specific.

Developmental stagei

Not detectable in females on the first day after eclosion, but is detectable on the second day. It is maximally expressed by day 4.

Interactioni

Protein-protein interaction databases

STRINGi7159.AAEL006347-PA.

Structurei

3D structure databases

ProteinModelPortaliP50635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni508 – 5147Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0737.
HOGENOMiHOG000247215.
InParanoidiP50635.
OMAiADGKDGF.
OrthoDBiEOG7TF78T.
PhylomeDBiP50635.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50635-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGRPGYSEV IFLYVVSVAV IARATDNMPV NKDVSKLFPL TLIHINDLHA
60 70 80 90 100
RFEETNMKSN VCTQKDQCIA GIARVYQKIK DLLKEYESKN PIYLNAGDNF
110 120 130 140 150
QGTLWYNLLR WNVTADFIKK LKPAAMTLGN HEFDHTPKGL APYLAELNKE
160 170 180 190 200
GIPTIVANLV MNNDPDLKSS KIPKSIKLTV GKRKIGIIGV LYDKTHEIAQ
210 220 230 240 250
TGKVTLSNAV EAVRREAAAL KKDNIDIIVV LSHCSYEEDK KIAAEAGDDI
260 270 280 290 300
DVIVGAHSHS FLYSPDSKQP HDPKDKVEGP YPTLVESKNK RKIPIVQAKS
310 320 330 340 350
FGKYVGRLTL YFDEEGEVKN WEGYPVFIDH KVQQDPQILK DLVPWRAKVE
360 370 380 390 400
AIGSTVVGET MIELDRDSCR DQECTLGVLY ADGFADQYTN DTFRPFAIIQ
410 420 430 440 450
AGNFRNPIKV GKITNGDIIE AAPFGSTADL IRLKGADIWD VAEHSFALDD
460 470 480 490 500
EGRTNCLQVS GLRIVIDISK PVRSRVKKIE VMDYTNPKSD KLKPLDKEAE
510 520 530 540 550
YYIVVPSYLA DGKDGFSAMK RATARRTGPL DSDVFKNYVE KIKKVDNLKL
560
GRVIVCKGSK CT
Length:562
Mass (Da):62,754
Last modified:July 1, 2008 - v2
Checksum:iEEC3C6BE34235EFD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301V → A in AAA99189. (PubMed:7498420)Curated
Sequence conflicti358 – 3581G → GG in AAA99189. (PubMed:7498420)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti9 – 91E → A in strain: Rockefeller.
Natural varianti30 – 301V → P in strain: Rockefeller.
Natural varianti61 – 611V → A in strain: Rockefeller.
Natural varianti224 – 2241N → K in strain: Rockefeller.
Natural varianti284 – 2841L → I in strain: Rockefeller.
Natural varianti314 – 3152EE → DT in strain: Rockefeller.
Natural varianti319 – 3202KN → QH in strain: Rockefeller.
Natural varianti347 – 3471A → E in strain: Rockefeller.
Natural varianti361 – 3611M → K in strain: Rockefeller.
Natural varianti472 – 4721V → I in strain: Rockefeller.
Natural varianti491 – 4911K → E in strain: Rockefeller.
Natural varianti498 – 4981E → Q in strain: Rockefeller.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12389 mRNA. Translation: AAC37218.1.
L41391 Genomic DNA. Translation: AAA99189.1.
CH477386 Genomic DNA. Translation: EAT42070.1.
RefSeqiXP_001651910.1. XM_001651860.1.
UniGeneiAae.5859.

Genome annotation databases

EnsemblMetazoaiAAEL006347-RA; AAEL006347-PA; AAEL006347.
GeneIDi5567877.
KEGGiaag:AaeL_AAEL006347.
VectorBaseiAAEL006347. Aedes aegypti.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L12389 mRNA. Translation: AAC37218.1 .
L41391 Genomic DNA. Translation: AAA99189.1 .
CH477386 Genomic DNA. Translation: EAT42070.1 .
RefSeqi XP_001651910.1. XM_001651860.1.
UniGenei Aae.5859.

3D structure databases

ProteinModelPortali P50635.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7159.AAEL006347-PA.

Protein family/group databases

Allergomei 3. Aed a 1.
3539. Aed a 1.0101.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai AAEL006347-RA ; AAEL006347-PA ; AAEL006347 .
GeneIDi 5567877.
KEGGi aag:AaeL_AAEL006347.
VectorBasei AAEL006347. Aedes aegypti.

Phylogenomic databases

eggNOGi COG0737.
HOGENOMi HOG000247215.
InParanoidi P50635.
OMAi ADGKDGF.
OrthoDBi EOG7TF78T.
PhylomeDBi P50635.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProi IPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
PANTHERi PTHR11575. PTHR11575. 1 hit.
Pfami PF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR01607. APYRASEFAMLY.
SUPFAMi SSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The salivary gland-specific apyrase of the mosquito Aedes aegypti is a member of the 5'-nucleotidase family."
    Champagne D.E., Smartt C.T., Ribeiro J.M.C., James A.A.
    Proc. Natl. Acad. Sci. U.S.A. 92:694-698(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 308-331 AND 498-521.
    Strain: Rockefeller.
    Tissue: Salivary gland.
  2. "The apyrase gene of the vector mosquito, Aedes aegypti, is expressed specifically in the adult female salivary glands."
    Smartt C.T., Kim A.P., Grossman G.L., James A.A.
    Exp. Parasitol. 81:239-248(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Rockefeller.
    Tissue: Salivary gland.
  3. "Genome sequence of Aedes aegypti, a major arbovirus vector."
    Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.
    , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
    Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LVPib12.

Entry informationi

Entry nameiAPY_AEDAE
AccessioniPrimary (citable) accession number: P50635
Secondary accession number(s): Q176L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3