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P50635 (APY_AEDAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apyrase

EC=3.6.1.5
Alternative name(s):
ATP-diphosphatase
Short name=ADPase
ATP-diphosphohydrolase
Adenosine diphosphatase
Allergen=Aed a 1
Gene names
Name:APY
ORF Names:AAEL006347
OrganismAedes aegypti (Yellowfever mosquito) (Culex aegypti) [Reference proteome]
Taxonomic identifier7159 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Facilitates hematophagy by preventing ADP-dependent platelet aggregation in the host. May reduce probing time by facilitating the speed of locating blood.

Catalytic activity

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactor

Divalent cations By similarity.

Subcellular location

Secreted.

Tissue specificity

Salivary gland specific.

Developmental stage

Not detectable in females on the first day after eclosion, but is detectable on the second day. It is maximally expressed by day 4.

Post-translational modification

The N-terminus is blocked.

Allergenic properties

Causes an allergic reaction in human.

Sequence similarities

Belongs to the 5'-nucleotidase family.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   DomainSignal
   LigandATP-binding
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processnucleotide catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrolase activity, acting on ester bonds

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 562538Apyrase
PRO_0000000039

Regions

Region508 – 5147Substrate binding By similarity

Sites

Metal binding471Divalent metal cation 1 By similarity
Metal binding491Divalent metal cation 1 By similarity
Metal binding981Divalent metal cation 1 By similarity
Metal binding981Divalent metal cation 2 By similarity
Metal binding1301Divalent metal cation 2 By similarity
Metal binding2331Divalent metal cation 2 By similarity
Metal binding2571Divalent metal cation 2 By similarity
Binding site4241Substrate By similarity
Site1311Transition state stabilizer By similarity
Site1341Transition state stabilizer By similarity

Amino acid modifications

Glycosylation1121N-linked (GlcNAc...) Potential
Glycosylation3901N-linked (GlcNAc...) Potential

Natural variations

Natural variant91E → A in strain: Rockefeller.
Natural variant301V → P in strain: Rockefeller.
Natural variant611V → A in strain: Rockefeller.
Natural variant2241N → K in strain: Rockefeller.
Natural variant2841L → I in strain: Rockefeller.
Natural variant314 – 3152EE → DT in strain: Rockefeller.
Natural variant319 – 3202KN → QH in strain: Rockefeller.
Natural variant3471A → E in strain: Rockefeller.
Natural variant3611M → K in strain: Rockefeller.
Natural variant4721V → I in strain: Rockefeller.
Natural variant4911K → E in strain: Rockefeller.
Natural variant4981E → Q in strain: Rockefeller.

Experimental info

Sequence conflict301V → A in AAA99189. Ref.2
Sequence conflict3581G → GG in AAA99189. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P50635 [UniParc].

Last modified July 1, 2008. Version 2.
Checksum: EEC3C6BE34235EFD

FASTA56262,754
        10         20         30         40         50         60 
MAGRPGYSEV IFLYVVSVAV IARATDNMPV NKDVSKLFPL TLIHINDLHA RFEETNMKSN 

        70         80         90        100        110        120 
VCTQKDQCIA GIARVYQKIK DLLKEYESKN PIYLNAGDNF QGTLWYNLLR WNVTADFIKK 

       130        140        150        160        170        180 
LKPAAMTLGN HEFDHTPKGL APYLAELNKE GIPTIVANLV MNNDPDLKSS KIPKSIKLTV 

       190        200        210        220        230        240 
GKRKIGIIGV LYDKTHEIAQ TGKVTLSNAV EAVRREAAAL KKDNIDIIVV LSHCSYEEDK 

       250        260        270        280        290        300 
KIAAEAGDDI DVIVGAHSHS FLYSPDSKQP HDPKDKVEGP YPTLVESKNK RKIPIVQAKS 

       310        320        330        340        350        360 
FGKYVGRLTL YFDEEGEVKN WEGYPVFIDH KVQQDPQILK DLVPWRAKVE AIGSTVVGET 

       370        380        390        400        410        420 
MIELDRDSCR DQECTLGVLY ADGFADQYTN DTFRPFAIIQ AGNFRNPIKV GKITNGDIIE 

       430        440        450        460        470        480 
AAPFGSTADL IRLKGADIWD VAEHSFALDD EGRTNCLQVS GLRIVIDISK PVRSRVKKIE 

       490        500        510        520        530        540 
VMDYTNPKSD KLKPLDKEAE YYIVVPSYLA DGKDGFSAMK RATARRTGPL DSDVFKNYVE 

       550        560 
KIKKVDNLKL GRVIVCKGSK CT 

« Hide

References

« Hide 'large scale' references
[1]"The salivary gland-specific apyrase of the mosquito Aedes aegypti is a member of the 5'-nucleotidase family."
Champagne D.E., Smartt C.T., Ribeiro J.M.C., James A.A.
Proc. Natl. Acad. Sci. U.S.A. 92:694-698(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 308-331 AND 498-521.
Strain: Rockefeller.
Tissue: Salivary gland.
[2]"The apyrase gene of the vector mosquito, Aedes aegypti, is expressed specifically in the adult female salivary glands."
Smartt C.T., Kim A.P., Grossman G.L., James A.A.
Exp. Parasitol. 81:239-248(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Rockefeller.
Tissue: Salivary gland.
[3]"Genome sequence of Aedes aegypti, a major arbovirus vector."
Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. expand/collapse author list , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LVPib12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L12389 mRNA. Translation: AAC37218.1.
L41391 Genomic DNA. Translation: AAA99189.1.
CH477386 Genomic DNA. Translation: EAT42070.1.
RefSeqXP_001651910.1. XM_001651860.1.
UniGeneAae.5859.

3D structure databases

ProteinModelPortalP50635.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7159.AAEL006347-PA.

Protein family/group databases

Allergome3. Aed a 1.
3539. Aed a 1.0101.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaAAEL006347-RA; AAEL006347-PA; AAEL006347.
GeneID5567877.
KEGGaag:AaeL_AAEL006347.
VectorBaseAAEL006347. Aedes aegypti.

Phylogenomic databases

eggNOGCOG0737.
HOGENOMHOG000247215.
InParanoidP50635.
OMAADGKDGF.
OrthoDBEOG7TF78T.
PhylomeDBP50635.

Family and domain databases

Gene3D3.90.780.10. 1 hit.
InterProIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
[Graphical view]
PANTHERPTHR11575. PTHR11575. 1 hit.
PfamPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR01607. APYRASEFAMLY.
SUPFAMSSF55816. SSF55816. 1 hit.
PROSITEPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPY_AEDAE
AccessionPrimary (citable) accession number: P50635
Secondary accession number(s): Q176L2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 1, 2008
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Allergens

Nomenclature of allergens and list of entries