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Protein

Apyrase

Gene

APY

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Facilitates hematophagy by preventing ADP-dependent platelet aggregation in the host. May reduce probing time by facilitating the speed of locating blood.

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Divalent metal cation 1By similarity1
Metal bindingi49Divalent metal cation 1By similarity1
Metal bindingi98Divalent metal cation 1By similarity1
Metal bindingi98Divalent metal cation 2By similarity1
Metal bindingi130Divalent metal cation 2By similarity1
Sitei131Transition state stabilizerBy similarity1
Sitei134Transition state stabilizerBy similarity1
Metal bindingi233Divalent metal cation 2By similarity1
Metal bindingi257Divalent metal cation 2By similarity1
Binding sitei424SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apyrase (EC:3.6.1.5)
Alternative name(s):
ATP-diphosphatase
Short name:
ADPase
ATP-diphosphohydrolase
Adenosine diphosphatase
Allergen: Aed a 1
Gene namesi
Name:APY
ORF Names:AAEL006347
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
Proteomesi
  • UP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3 Aed a 1
3539 Aed a 1.0101

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000003925 – 562ApyraseAdd BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi112N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi390N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Salivary gland specific.

Developmental stagei

Not detectable in females on the first day after eclosion, but is detectable on the second day. It is maximally expressed by day 4.

Interactioni

Protein-protein interaction databases

STRINGi7159.AAEL006347-PA

Structurei

3D structure databases

ProteinModelPortaliP50635
SMRiP50635
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni508 – 514Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4419 Eukaryota
COG0737 LUCA
HOGENOMiHOG000247215
InParanoidiP50635
OMAiLKWNITQ
OrthoDBiEOG091G08IK
PhylomeDBiP50635

Family and domain databases

Gene3Di3.60.21.101 hit
3.90.780.101 hit
InterProiView protein in InterPro
IPR008334 5'-Nucleotdase_C
IPR036907 5'-Nucleotdase_C_sf
IPR006146 5'-Nucleotdase_CS
IPR006179 5_nucleotidase/apyrase
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
PANTHERiPTHR11575 PTHR11575, 1 hit
PfamiView protein in Pfam
PF02872 5_nucleotid_C, 1 hit
PF00149 Metallophos, 1 hit
PRINTSiPR01607 APYRASEFAMLY
SUPFAMiSSF55816 SSF55816, 1 hit
PROSITEiView protein in PROSITE
PS00785 5_NUCLEOTIDASE_1, 1 hit
PS00786 5_NUCLEOTIDASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRPGYSEV IFLYVVSVAV IARATDNMPV NKDVSKLFPL TLIHINDLHA
60 70 80 90 100
RFEETNMKSN VCTQKDQCIA GIARVYQKIK DLLKEYESKN PIYLNAGDNF
110 120 130 140 150
QGTLWYNLLR WNVTADFIKK LKPAAMTLGN HEFDHTPKGL APYLAELNKE
160 170 180 190 200
GIPTIVANLV MNNDPDLKSS KIPKSIKLTV GKRKIGIIGV LYDKTHEIAQ
210 220 230 240 250
TGKVTLSNAV EAVRREAAAL KKDNIDIIVV LSHCSYEEDK KIAAEAGDDI
260 270 280 290 300
DVIVGAHSHS FLYSPDSKQP HDPKDKVEGP YPTLVESKNK RKIPIVQAKS
310 320 330 340 350
FGKYVGRLTL YFDEEGEVKN WEGYPVFIDH KVQQDPQILK DLVPWRAKVE
360 370 380 390 400
AIGSTVVGET MIELDRDSCR DQECTLGVLY ADGFADQYTN DTFRPFAIIQ
410 420 430 440 450
AGNFRNPIKV GKITNGDIIE AAPFGSTADL IRLKGADIWD VAEHSFALDD
460 470 480 490 500
EGRTNCLQVS GLRIVIDISK PVRSRVKKIE VMDYTNPKSD KLKPLDKEAE
510 520 530 540 550
YYIVVPSYLA DGKDGFSAMK RATARRTGPL DSDVFKNYVE KIKKVDNLKL
560
GRVIVCKGSK CT
Length:562
Mass (Da):62,754
Last modified:July 1, 2008 - v2
Checksum:iEEC3C6BE34235EFD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30V → A in AAA99189 (PubMed:7498420).Curated1
Sequence conflicti358G → GG in AAA99189 (PubMed:7498420).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti9E → A in strain: Rockefeller. 1
Natural varianti30V → P in strain: Rockefeller. 1
Natural varianti61V → A in strain: Rockefeller. 1
Natural varianti224N → K in strain: Rockefeller. 1
Natural varianti284L → I in strain: Rockefeller. 1
Natural varianti314 – 315EE → DT in strain: Rockefeller. 2
Natural varianti319 – 320KN → QH in strain: Rockefeller. 2
Natural varianti347A → E in strain: Rockefeller. 1
Natural varianti361M → K in strain: Rockefeller. 1
Natural varianti472V → I in strain: Rockefeller. 1
Natural varianti491K → E in strain: Rockefeller. 1
Natural varianti498E → Q in strain: Rockefeller. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12389 mRNA Translation: AAC37218.1
L41391 Genomic DNA Translation: AAA99189.1
CH477386 Genomic DNA Translation: EAT42070.1
RefSeqiXP_001651910.1, XM_001651860.1
UniGeneiAae.5859

Genome annotation databases

EnsemblMetazoaiAAEL006347-RA; AAEL006347-PA; AAEL006347
GeneIDi5567877
KEGGiaag:5567877

Similar proteinsi

Entry informationi

Entry nameiAPY_AEDAE
AccessioniPrimary (citable) accession number: P50635
Secondary accession number(s): Q176L2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 1, 2008
Last modified: March 28, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome