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P50635

- APY_AEDAE

UniProt

P50635 - APY_AEDAE

Protein

Apyrase

Gene

APY

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Facilitates hematophagy by preventing ADP-dependent platelet aggregation in the host. May reduce probing time by facilitating the speed of locating blood.

    Catalytic activityi

    A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

    Cofactori

    Divalent cations.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Divalent metal cation 1By similarity
    Metal bindingi49 – 491Divalent metal cation 1By similarity
    Metal bindingi98 – 981Divalent metal cation 1By similarity
    Metal bindingi98 – 981Divalent metal cation 2By similarity
    Metal bindingi130 – 1301Divalent metal cation 2By similarity
    Sitei131 – 1311Transition state stabilizerBy similarity
    Sitei134 – 1341Transition state stabilizerBy similarity
    Metal bindingi233 – 2331Divalent metal cation 2By similarity
    Metal bindingi257 – 2571Divalent metal cation 2By similarity
    Binding sitei424 – 4241SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrolase activity, acting on ester bonds Source: InterPro
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. nucleotide catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apyrase (EC:3.6.1.5)
    Alternative name(s):
    ATP-diphosphatase
    Short name:
    ADPase
    ATP-diphosphohydrolase
    Adenosine diphosphatase
    Allergen: Aed a 1
    Gene namesi
    Name:APY
    ORF Names:AAEL006347
    OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
    Taxonomic identifieri7159 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
    ProteomesiUP000008820: Unassembled WGS sequence

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei3539. Aed a 1.0101.
    3. Aed a 1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 562538ApyrasePRO_0000000039Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Salivary gland specific.

    Developmental stagei

    Not detectable in females on the first day after eclosion, but is detectable on the second day. It is maximally expressed by day 4.

    Interactioni

    Protein-protein interaction databases

    STRINGi7159.AAEL006347-PA.

    Structurei

    3D structure databases

    ProteinModelPortaliP50635.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni508 – 5147Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the 5'-nucleotidase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0737.
    HOGENOMiHOG000247215.
    InParanoidiP50635.
    OMAiADGKDGF.
    OrthoDBiEOG7TF78T.
    PhylomeDBiP50635.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProiIPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PANTHERiPTHR11575. PTHR11575. 1 hit.
    PfamiPF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR01607. APYRASEFAMLY.
    SUPFAMiSSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
    PS00786. 5_NUCLEOTIDASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50635-1 [UniParc]FASTAAdd to Basket

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    MAGRPGYSEV IFLYVVSVAV IARATDNMPV NKDVSKLFPL TLIHINDLHA    50
    RFEETNMKSN VCTQKDQCIA GIARVYQKIK DLLKEYESKN PIYLNAGDNF 100
    QGTLWYNLLR WNVTADFIKK LKPAAMTLGN HEFDHTPKGL APYLAELNKE 150
    GIPTIVANLV MNNDPDLKSS KIPKSIKLTV GKRKIGIIGV LYDKTHEIAQ 200
    TGKVTLSNAV EAVRREAAAL KKDNIDIIVV LSHCSYEEDK KIAAEAGDDI 250
    DVIVGAHSHS FLYSPDSKQP HDPKDKVEGP YPTLVESKNK RKIPIVQAKS 300
    FGKYVGRLTL YFDEEGEVKN WEGYPVFIDH KVQQDPQILK DLVPWRAKVE 350
    AIGSTVVGET MIELDRDSCR DQECTLGVLY ADGFADQYTN DTFRPFAIIQ 400
    AGNFRNPIKV GKITNGDIIE AAPFGSTADL IRLKGADIWD VAEHSFALDD 450
    EGRTNCLQVS GLRIVIDISK PVRSRVKKIE VMDYTNPKSD KLKPLDKEAE 500
    YYIVVPSYLA DGKDGFSAMK RATARRTGPL DSDVFKNYVE KIKKVDNLKL 550
    GRVIVCKGSK CT 562
    Length:562
    Mass (Da):62,754
    Last modified:July 1, 2008 - v2
    Checksum:iEEC3C6BE34235EFD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 301V → A in AAA99189. (PubMed:7498420)Curated
    Sequence conflicti358 – 3581G → GG in AAA99189. (PubMed:7498420)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti9 – 91E → A in strain: Rockefeller.
    Natural varianti30 – 301V → P in strain: Rockefeller.
    Natural varianti61 – 611V → A in strain: Rockefeller.
    Natural varianti224 – 2241N → K in strain: Rockefeller.
    Natural varianti284 – 2841L → I in strain: Rockefeller.
    Natural varianti314 – 3152EE → DT in strain: Rockefeller.
    Natural varianti319 – 3202KN → QH in strain: Rockefeller.
    Natural varianti347 – 3471A → E in strain: Rockefeller.
    Natural varianti361 – 3611M → K in strain: Rockefeller.
    Natural varianti472 – 4721V → I in strain: Rockefeller.
    Natural varianti491 – 4911K → E in strain: Rockefeller.
    Natural varianti498 – 4981E → Q in strain: Rockefeller.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12389 mRNA. Translation: AAC37218.1.
    L41391 Genomic DNA. Translation: AAA99189.1.
    CH477386 Genomic DNA. Translation: EAT42070.1.
    RefSeqiXP_001651910.1. XM_001651860.1.
    UniGeneiAae.5859.

    Genome annotation databases

    EnsemblMetazoaiAAEL006347-RA; AAEL006347-PA; AAEL006347.
    GeneIDi5567877.
    KEGGiaag:AaeL_AAEL006347.
    VectorBaseiAAEL006347. Aedes aegypti.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L12389 mRNA. Translation: AAC37218.1 .
    L41391 Genomic DNA. Translation: AAA99189.1 .
    CH477386 Genomic DNA. Translation: EAT42070.1 .
    RefSeqi XP_001651910.1. XM_001651860.1.
    UniGenei Aae.5859.

    3D structure databases

    ProteinModelPortali P50635.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7159.AAEL006347-PA.

    Protein family/group databases

    Allergomei 3539. Aed a 1.0101.
    3. Aed a 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai AAEL006347-RA ; AAEL006347-PA ; AAEL006347 .
    GeneIDi 5567877.
    KEGGi aag:AaeL_AAEL006347.
    VectorBasei AAEL006347. Aedes aegypti.

    Phylogenomic databases

    eggNOGi COG0737.
    HOGENOMi HOG000247215.
    InParanoidi P50635.
    OMAi ADGKDGF.
    OrthoDBi EOG7TF78T.
    PhylomeDBi P50635.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProi IPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    PANTHERi PTHR11575. PTHR11575. 1 hit.
    Pfami PF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR01607. APYRASEFAMLY.
    SUPFAMi SSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
    PS00786. 5_NUCLEOTIDASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The salivary gland-specific apyrase of the mosquito Aedes aegypti is a member of the 5'-nucleotidase family."
      Champagne D.E., Smartt C.T., Ribeiro J.M.C., James A.A.
      Proc. Natl. Acad. Sci. U.S.A. 92:694-698(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 308-331 AND 498-521.
      Strain: Rockefeller.
      Tissue: Salivary gland.
    2. "The apyrase gene of the vector mosquito, Aedes aegypti, is expressed specifically in the adult female salivary glands."
      Smartt C.T., Kim A.P., Grossman G.L., James A.A.
      Exp. Parasitol. 81:239-248(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Rockefeller.
      Tissue: Salivary gland.
    3. "Genome sequence of Aedes aegypti, a major arbovirus vector."
      Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.
      , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.
      Science 316:1718-1723(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LVPib12.

    Entry informationi

    Entry nameiAPY_AEDAE
    AccessioniPrimary (citable) accession number: P50635
    Secondary accession number(s): Q176L2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3