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Protein

Apyrase

Gene

APY

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Facilitates hematophagy by preventing ADP-dependent platelet aggregation in the host. May reduce probing time by facilitating the speed of locating blood.

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Divalent metal cation 1By similarity1
Metal bindingi49Divalent metal cation 1By similarity1
Metal bindingi98Divalent metal cation 1By similarity1
Metal bindingi98Divalent metal cation 2By similarity1
Metal bindingi130Divalent metal cation 2By similarity1
Sitei131Transition state stabilizerBy similarity1
Sitei134Transition state stabilizerBy similarity1
Metal bindingi233Divalent metal cation 2By similarity1
Metal bindingi257Divalent metal cation 2By similarity1
Binding sitei424SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apyrase (EC:3.6.1.5)
Alternative name(s):
ATP-diphosphatase
Short name:
ADPase
ATP-diphosphohydrolase
Adenosine diphosphatase
Allergen: Aed a 1
Gene namesi
Name:APY
ORF Names:AAEL006347
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
Proteomesi
  • UP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3. Aed a 1.
3539. Aed a 1.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000003925 – 562ApyraseAdd BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi112N-linked (GlcNAc...)Sequence analysis1
Glycosylationi390N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Salivary gland specific.

Developmental stagei

Not detectable in females on the first day after eclosion, but is detectable on the second day. It is maximally expressed by day 4.

Interactioni

Protein-protein interaction databases

STRINGi7159.AAEL006347-PA.

Structurei

3D structure databases

ProteinModelPortaliP50635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni508 – 514Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4419. Eukaryota.
COG0737. LUCA.
HOGENOMiHOG000247215.
InParanoidiP50635.
OMAiGRTNCLQ.
OrthoDBiEOG091G08IK.
PhylomeDBiP50635.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRPGYSEV IFLYVVSVAV IARATDNMPV NKDVSKLFPL TLIHINDLHA
60 70 80 90 100
RFEETNMKSN VCTQKDQCIA GIARVYQKIK DLLKEYESKN PIYLNAGDNF
110 120 130 140 150
QGTLWYNLLR WNVTADFIKK LKPAAMTLGN HEFDHTPKGL APYLAELNKE
160 170 180 190 200
GIPTIVANLV MNNDPDLKSS KIPKSIKLTV GKRKIGIIGV LYDKTHEIAQ
210 220 230 240 250
TGKVTLSNAV EAVRREAAAL KKDNIDIIVV LSHCSYEEDK KIAAEAGDDI
260 270 280 290 300
DVIVGAHSHS FLYSPDSKQP HDPKDKVEGP YPTLVESKNK RKIPIVQAKS
310 320 330 340 350
FGKYVGRLTL YFDEEGEVKN WEGYPVFIDH KVQQDPQILK DLVPWRAKVE
360 370 380 390 400
AIGSTVVGET MIELDRDSCR DQECTLGVLY ADGFADQYTN DTFRPFAIIQ
410 420 430 440 450
AGNFRNPIKV GKITNGDIIE AAPFGSTADL IRLKGADIWD VAEHSFALDD
460 470 480 490 500
EGRTNCLQVS GLRIVIDISK PVRSRVKKIE VMDYTNPKSD KLKPLDKEAE
510 520 530 540 550
YYIVVPSYLA DGKDGFSAMK RATARRTGPL DSDVFKNYVE KIKKVDNLKL
560
GRVIVCKGSK CT
Length:562
Mass (Da):62,754
Last modified:July 1, 2008 - v2
Checksum:iEEC3C6BE34235EFD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30V → A in AAA99189 (PubMed:7498420).Curated1
Sequence conflicti358G → GG in AAA99189 (PubMed:7498420).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti9E → A in strain: Rockefeller. 1
Natural varianti30V → P in strain: Rockefeller. 1
Natural varianti61V → A in strain: Rockefeller. 1
Natural varianti224N → K in strain: Rockefeller. 1
Natural varianti284L → I in strain: Rockefeller. 1
Natural varianti314 – 315EE → DT in strain: Rockefeller. 2
Natural varianti319 – 320KN → QH in strain: Rockefeller. 2
Natural varianti347A → E in strain: Rockefeller. 1
Natural varianti361M → K in strain: Rockefeller. 1
Natural varianti472V → I in strain: Rockefeller. 1
Natural varianti491K → E in strain: Rockefeller. 1
Natural varianti498E → Q in strain: Rockefeller. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12389 mRNA. Translation: AAC37218.1.
L41391 Genomic DNA. Translation: AAA99189.1.
CH477386 Genomic DNA. Translation: EAT42070.1.
RefSeqiXP_001651910.1. XM_001651860.1.
UniGeneiAae.5859.

Genome annotation databases

EnsemblMetazoaiAAEL006347-RA; AAEL006347-PA; AAEL006347.
GeneIDi5567877.
KEGGiaag:AaeL_AAEL006347.
VectorBaseiAAEL006347-RA; AAEL006347-PA; AAEL006347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12389 mRNA. Translation: AAC37218.1.
L41391 Genomic DNA. Translation: AAA99189.1.
CH477386 Genomic DNA. Translation: EAT42070.1.
RefSeqiXP_001651910.1. XM_001651860.1.
UniGeneiAae.5859.

3D structure databases

ProteinModelPortaliP50635.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7159.AAEL006347-PA.

Protein family/group databases

Allergomei3. Aed a 1.
3539. Aed a 1.0101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAAEL006347-RA; AAEL006347-PA; AAEL006347.
GeneIDi5567877.
KEGGiaag:AaeL_AAEL006347.
VectorBaseiAAEL006347-RA; AAEL006347-PA; AAEL006347.

Organism-specific databases

CTDi5567877.

Phylogenomic databases

eggNOGiKOG4419. Eukaryota.
COG0737. LUCA.
HOGENOMiHOG000247215.
InParanoidiP50635.
OMAiGRTNCLQ.
OrthoDBiEOG091G08IK.
PhylomeDBiP50635.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPY_AEDAE
AccessioniPrimary (citable) accession number: P50635
Secondary accession number(s): Q176L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 1, 2008
Last modified: November 30, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.