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Protein

SUMO-conjugating enzyme UBC9

Gene

UBC9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2).2 Publications

Miscellaneous

Present with 2600 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

SUMOyl-[E2 SUMO-conjugating enzyme]-L-cysteine + [acceptor-protein]-L-lysine = [E2 SUMO-conjugating enzyme]-L-cysteine + N6-SUMOyl-[acceptor protein]-L-lysine.

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei93Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • SUMO transferase activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic spindle elongation Source: SGD
  • protein sumoylation Source: SGD

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29479-MONOMER
BRENDAi2.3.2.B5 984
ReactomeiR-SCE-3065678 SUMO is transferred from E1 to E2 (UBE2I, UBC9)
R-SCE-3108214 SUMOylation of DNA damage response and repair proteins
R-SCE-3899300 SUMOylation of transcription cofactors
R-SCE-4551638 SUMOylation of chromatin organization proteins
R-SCE-4570464 SUMOylation of RNA binding proteins
R-SCE-4615885 SUMOylation of DNA replication proteins
R-SCE-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-SCE-5696395 Formation of Incision Complex in GG-NER
UniPathwayiUPA00886

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-conjugating enzyme UBC9 (EC:2.3.2.-)
Alternative name(s):
RING-type E3 SUMO transferase UBC9
Ubiquitin carrier protein 9
Ubiquitin-conjugating enzyme E2-18 kDa
Ubiquitin-protein ligase
Gene namesi
Name:UBC9
Ordered Locus Names:YDL064W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL064W
SGDiS000002222 UBC9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000825562 – 157SUMO-conjugating enzyme UBC9Add BLAST156

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP50623
PaxDbiP50623
PRIDEiP50623

PTM databases

iPTMnetiP50623

Interactioni

Subunit structurei

Interacts with SIZ1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SMT3Q123063EBI-19760,EBI-17490

Protein-protein interaction databases

BioGridi31995, 289 interactors
DIPiDIP-1531N
IntActiP50623, 8 interactors
MINTiP50623
STRINGi4932.YDL064W

Structurei

Secondary structure

1157
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Beta strandi25 – 30Combined sources6
Beta strandi36 – 46Combined sources11
Turni52 – 55Combined sources4
Beta strandi56 – 63Combined sources8
Turni66 – 70Combined sources5
Beta strandi74 – 76Combined sources3
Beta strandi90 – 92Combined sources3
Helixi95 – 97Combined sources3
Turni99 – 102Combined sources4
Helixi109 – 120Combined sources12
Helixi131 – 139Combined sources9
Helixi141 – 154Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EKEX-ray1.90A/B1-157[»]
2GJDX-ray1.75A/B/C/D1-157[»]
3ONGX-ray2.30B/D1-157[»]
5JNEX-ray2.85B/F1-157[»]
ProteinModelPortaliP50623
SMRiP50623
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50623

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000075088
HOGENOMiHOG000233454
InParanoidiP50623
KOiK10577
OMAiKCKFTPP
OrthoDBiEOG092C50OL

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR027230 Ubc9
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PANTHERiPTHR43927 PTHR43927, 1 hit
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLCLQRLQ EERKKWRKDH PFGFYAKPVK KADGSMDLQK WEAGIPGKEG
60 70 80 90 100
TNWAGGVYPI TVEYPNEYPS KPPKVKFPAG FYHPNVYPSG TICLSILNED
110 120 130 140 150
QDWRPAITLK QIVLGVQDLL DSPNPNSPAQ EPAWRSFSRN KAEYDKKVLL

QAKQYSK
Length:157
Mass (Da):17,911
Last modified:October 1, 1996 - v1
Checksum:iD74EE9845BB677B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82538 Genomic DNA Translation: CAA57888.1
Z74112 Genomic DNA Translation: CAA98629.1
BK006938 Genomic DNA Translation: DAA11793.1
PIRiS52414
RefSeqiNP_010219.1, NM_001180123.1

Genome annotation databases

EnsemblFungiiYDL064W; YDL064W; YDL064W
GeneIDi851495
KEGGisce:YDL064W

Similar proteinsi

Entry informationi

Entry nameiUBC9_YEAST
AccessioniPrimary (citable) accession number: P50623
Secondary accession number(s): D6VRT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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