Reviewed,
UniProtKB/Swiss-Prot P50623 (UBC9_YEAST)
Last modified
November 24, 2009.
Version 80.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: SUMO-conjugating enzyme UBC9 EC=6.3.2.- Alternative name(s): Ubiquitin-conjugating enzyme E2-18 kDa Ubiquitin-protein ligase Ubiquitin carrier protein 9 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 157 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.3 Ref.4 |
| Catalytic activity | ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine. |
| Pathway | |
| Subunit structure | |
| Subcellular location | |
| Miscellaneous | Present with 2600 molecules/cell in log phase SD medium. Ref.7 |
| Sequence similarities | Belongs to the ubiquitin-conjugating enzyme family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 2 | EBI-19760,EBI-19760 | ||
| SMT3 | Q12306 | 2 | EBI-19760,EBI-17490 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 157 | 157 | SUMO-conjugating enzyme UBC9 | PRO_0000082556 | |||||||||||||||||||||||||||||
Sites | |||||||||||||||||||||||||||||||||
| Active site | 93 | 1 | Glycyl thioester intermediate By similarity | ||||||||||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||||||||||
| Helix | 4 – 18 | 15 | |||||||||||||||||||||||||||||||
| Beta strand | 25 – 30 | 6 | |||||||||||||||||||||||||||||||
| Beta strand | 36 – 46 | 11 | |||||||||||||||||||||||||||||||
| Turn | 52 – 55 | 4 | |||||||||||||||||||||||||||||||
| Beta strand | 57 – 63 | 7 | |||||||||||||||||||||||||||||||
| Turn | 66 – 70 | 5 | |||||||||||||||||||||||||||||||
| Beta strand | 74 – 76 | 3 | |||||||||||||||||||||||||||||||
| Helix | 95 – 97 | 3 | |||||||||||||||||||||||||||||||
| Turn | 99 – 102 | 4 | |||||||||||||||||||||||||||||||
| Helix | 109 – 121 | 13 | |||||||||||||||||||||||||||||||
| Helix | 131 – 139 | 9 | |||||||||||||||||||||||||||||||
| Helix | 141 – 154 | 14 | |||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Role of a ubiquitin-conjugating enzyme in degradation of S- and M-phase cyclins." Seufert W., Futcher B., Jentsch S. Nature 373:78-81(1995) [PubMed: 7800043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P.Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p." Johnson E.S., Blobel G. J. Biol. Chem. 272:26799-26802(1997) [PubMed: 9341106] [Abstract] Cited for: FUNCTION. |
| [4] | "An E3-like factor that promotes SUMO conjugation to the yeast septins." Johnson E.S., Gupta A.A. Cell 106:735-744(2001) [PubMed: 11572779] [Abstract] Cited for: INTERACTION WITH SIZ1, FUNCTION. |
| [5] | "A novel factor required for the SUMO1/Smt3 conjugation of yeast septins." Takahashi Y., Toh-e A., Kikuchi Y. Gene 275:223-231(2001) [PubMed: 11587849] [Abstract] Cited for: INTERACTION WITH SIZ1. |
| [6] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [7] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X82538 Genomic DNA. Translation: CAA57888.1. Z74112 Genomic DNA. Translation: CAA98629.1. | |||||||||||||||||||
| PIR | S52414. | ||||||||||||||||||
| RefSeq | NP_010219.1. | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| DIP | DIP:1531N. | ||||||||||||||||||
| IntAct | P50623. 7 interactions. | ||||||||||||||||||
| STRING | P50623. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PeptideAtlas | P50623. | ||||||||||||||||||
| PRIDE | P50623. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | YDL064W; YDL064W; YDL064W; Saccharomyces cerevisiae. [Genome view] | ||||||||||||||||||
| GeneID | 851495. | ||||||||||||||||||
| KEGG | sce:YDL064W. | ||||||||||||||||||
| NMPDR | fig|4932.3.peg.959. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CYGD | YDL064w. | ||||||||||||||||||
| SGD | S000002222. UBC9. | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOGENOM | P50623. | ||||||||||||||||||
| OMA | KQWRKDH | ||||||||||||||||||
| OrthoDB | EOG9QVDVD | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P50623. | ||||||||||||||||||
| Genevestigator | P50623. | ||||||||||||||||||
| GermOnline | YDL064W. Saccharomyces cerevisiae. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view] | ||||||||||||||||||
| Gene3D | G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit. | ||||||||||||||||||
| Pfam | PF00179. UQ_con. 1 hit. [Graphical view] | ||||||||||||||||||
| SMART | SM00212. UBCc. 1 hit. [Graphical view] | ||||||||||||||||||
| PROSITE | PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| NextBio | 968831. | ||||||||||||||||||
Entry information
| Entry name | UBC9_YEAST | ||||||||
| Accession | Primary (citable) accession number: P50623 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


