Reviewed, UniProtKB/Swiss-Prot P50622 (LIG6_PHACH)

Names and origin

Protein names

Recommended name:
    Ligninase LG6
    EC=1.11.1.14
Alternative name(s):
    Diarylpropane peroxidase
    Lignin peroxidase

Gene names

Name:

GLG6

Organism

Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)

Taxonomic identifier

5306 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Corticiales › Corticiaceae › Phanerochaete

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.
Catalytic activity	1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol + H2O2 = 3,4-dimethoxybenzaldehyde + 1-(3,4-dimethoxyphenyl)ethane-1,2-diol + H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 2 calcium ions per subunit By similarity.
Pathway	Secondary metabolite metabolism; lignin degradation.
Sequence similarities	Belongs to the peroxidase family. Ligninase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide Lignin degradation
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diarylpropane peroxidase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 28	7	Potential		PRO_0000023770
	Chain	29 – 372	344	Ligninase LG6		PRO_0000023771
Sites
	Active site	75	1	Proton acceptor By similarity
	Metal binding	76	1	Calcium 1 By similarity
	Metal binding	94	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	96	1	Calcium 1 By similarity
	Metal binding	98	1	Calcium 1 By similarity
	Metal binding	204	1	Iron (heme axial ligand) By similarity
	Metal binding	205	1	Calcium 2 By similarity
	Metal binding	222	1	Calcium 2 By similarity
	Metal binding	224	1	Calcium 2 By similarity
	Metal binding	227	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	229	1	Calcium 2 By similarity
	Site	71	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	285	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 43		By similarity
	Disulfide bond	62 ↔ 148		By similarity
	Disulfide bond	277 ↔ 345		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50622-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B192A93351F8CC16	FASTA	372	39,259
	10 20 30 40 50 60 MALKQLAAAV ALALSIQAAQ GAAVKEKRAT CSNGATVGDA SSCAWFDVLD DIQQNLFNGA 70 80 90 100 110 120 QCGAEAHESI RLVFHDAIAI SPALESQGKF GGGGADGSII LFDDIETNFH PNIGLDEIVN 130 140 150 160 170 180 LQKPFIQKHG VTPGDFIAFA GAVAMSNCPG APQMNFFTGR APATQAAPDG LVPEPFHTVD 190 200 210 220 230 240 QIISRVNDAG EFDELELVWM LSAHSVAAAN DVDPTIQGLA FDSTPGVFDS QFFVETQLRG 250 260 270 280 290 300 TAFPGSGGNQ GEVESPLPGE MRLQSDSSIA RDSRTACEWQ SFVNNQSKLV SDFQFIFLAL 310 320 330 340 350 360 TQLGENPDAM TDCSDVIPIS KPVPNNVPFS FFPAGKTMAD VEQACAETPF PTLTTLPGPE 370 TSVQRIQPPP GA

References

[1]

"Characterization of a new lignin peroxidase gene (GLG6) from Phanerochaete chrysosporium." Naidu P.S., Zhang Y.Z., Reddy C.A. Biochem. Biophys. Res. Commun. 173:994-1000(1990) [PubMed: 2268360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 24725 / CBS 481.73 / CCRC 36200 / NRRL 6361 / VKM-F-1767.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M80213 mRNA. Translation: AAA33737.1.
3D structure databases
HSSP	HSSP built from PDB template 1B80 based on UniProtKB P06181.
SMR	P50622. Positions 24-372.
ModBase	Search...
Protein family/group databases
PeroxiBase	2415. PcLiPF.
Enzyme and pathway databases
BRENDA	1.11.1.14. 16698.
Family and domain databases
InterPro	IPR002016. Haem_peroxidase_pln/fun/bac. IPR001621. Ligninase. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00462. LIGNINASE. PR00458. PEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIG6_PHACH

Accession

Primary (citable) accession number: P50622

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 5, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)