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Protein

Ligninase LG6

Gene

GLG6

Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.By similarity

Catalytic activityi

1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = 3,4-dimethoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H2O.By similarity
2 (3,4-dimethoxyphenyl)methanol + H2O2 = 2 (3,4-dimethoxyphenyl)methanol radical + 2 H2O.By similarity

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Pathwayi: lignin degradation

This protein is involved in the pathway lignin degradation, which is part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the pathway lignin degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei71Transition state stabilizerPROSITE-ProRule annotation1
Active sitei75Proton acceptorPROSITE-ProRule annotation1
Metal bindingi76Calcium 1PROSITE-ProRule annotation1
Metal bindingi94Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi96Calcium 1PROSITE-ProRule annotation1
Metal bindingi98Calcium 1PROSITE-ProRule annotation1
Metal bindingi204Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi205Calcium 2PROSITE-ProRule annotation1
Metal bindingi222Calcium 2PROSITE-ProRule annotation1
Metal bindingi224Calcium 2PROSITE-ProRule annotation1
Metal bindingi227Calcium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi229Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Lignin degradation

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14342.
UniPathwayiUPA00892.

Protein family/group databases

CAZyiAA2. Auxiliary Activities 2.
PeroxiBasei2415. PcLiP06.

Names & Taxonomyi

Protein namesi
Recommended name:
Ligninase LG6 (EC:1.11.1.14By similarity)
Alternative name(s):
Diarylpropane peroxidase
Lignin peroxidase
Gene namesi
Name:GLG6
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002377022 – 28Sequence analysis7
ChainiPRO_000002377129 – 372Ligninase LG6Add BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 43PROSITE-ProRule annotation
Disulfide bondi62 ↔ 148PROSITE-ProRule annotation
Disulfide bondi277 ↔ 345PROSITE-ProRule annotation
Glycosylationi285N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP50622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Ligninase subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410II1B. Eukaryota.
ENOG410ZS1R. LUCA.

Family and domain databases

CDDicd00692. ligninase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR024589. Ligninase_C.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
PF11895. Peroxidase_ext. 1 hit.
[Graphical view]
PRINTSiPR00462. LIGNINASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKQLAAAV ALALSIQAAQ GAAVKEKRAT CSNGATVGDA SSCAWFDVLD
60 70 80 90 100
DIQQNLFNGA QCGAEAHESI RLVFHDAIAI SPALESQGKF GGGGADGSII
110 120 130 140 150
LFDDIETNFH PNIGLDEIVN LQKPFIQKHG VTPGDFIAFA GAVAMSNCPG
160 170 180 190 200
APQMNFFTGR APATQAAPDG LVPEPFHTVD QIISRVNDAG EFDELELVWM
210 220 230 240 250
LSAHSVAAAN DVDPTIQGLA FDSTPGVFDS QFFVETQLRG TAFPGSGGNQ
260 270 280 290 300
GEVESPLPGE MRLQSDSSIA RDSRTACEWQ SFVNNQSKLV SDFQFIFLAL
310 320 330 340 350
TQLGENPDAM TDCSDVIPIS KPVPNNVPFS FFPAGKTMAD VEQACAETPF
360 370
PTLTTLPGPE TSVQRIQPPP GA
Length:372
Mass (Da):39,259
Last modified:October 1, 1996 - v1
Checksum:iB192A93351F8CC16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80213 mRNA. Translation: AAA33737.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80213 mRNA. Translation: AAA33737.1.

3D structure databases

ProteinModelPortaliP50622.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiAA2. Auxiliary Activities 2.
PeroxiBasei2415. PcLiP06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410II1B. Eukaryota.
ENOG410ZS1R. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00892.
BioCyciMetaCyc:MONOMER-14342.

Family and domain databases

CDDicd00692. ligninase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR024589. Ligninase_C.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
PF11895. Peroxidase_ext. 1 hit.
[Graphical view]
PRINTSiPR00462. LIGNINASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIG6_PHACH
AccessioniPrimary (citable) accession number: P50622
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.