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Protein

Protein Tob1

Gene

TOB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex (PubMed:8632892, PubMed:23236473). Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay (PubMed:21336257).3 Publications

GO - Molecular functioni

  • receptor tyrosine kinase binding Source: UniProtKB
  • SH3/SH2 adaptor activity Source: ProtInc
  • transcription corepressor activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000141232-MONOMER.
SignaLinkiP50616.
SIGNORiP50616.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Tob1
Alternative name(s):
Transducer of erbB-2 1
Gene namesi
Name:TOB1
Synonyms:TOB, TROB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:11979. TOB1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Only a small fraction localizes to the cytoplasm except in late S-phase where more than half of proteins become cytoplasmic.By similarity

GO - Cellular componenti

  • CCR4-NOT complex Source: UniProtKB
  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45Y → A: Impairs interaction with CNOT7 and CNOT8. 1 Publication1
Mutagenesisi55F → A: Impairs interaction with CNOT7 and CNOT8. 1 Publication1
Mutagenesisi65D → A: Impairs interaction with CNOT7 and CNOT8. 1 Publication1
Mutagenesisi93W → A: Impairs interaction with CNOT7 and CNOT8. 1 Publication1
Mutagenesisi95D → A: Impairs interaction with CNOT7 and CNOT8. 1 Publication1

Organism-specific databases

DisGeNETi10140.
OpenTargetsiENSG00000141232.
PharmGKBiPA36663.

Polymorphism and mutation databases

BioMutaiTOB1.
DMDMi1729989.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001438121 – 345Protein Tob1Add BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei204PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated on Ser and Thr residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP50616.
PaxDbiP50616.
PeptideAtlasiP50616.
PRIDEiP50616.

PTM databases

iPTMnetiP50616.
PhosphoSitePlusiP50616.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000141232.
CleanExiHS_TOB1.
GenevisibleiP50616. HS.

Organism-specific databases

HPAiCAB010908.
HPA047839.

Interactioni

Subunit structurei

Interacts with ERBB2 (PubMed:8632892). Interacts with CNOT7 (PubMed:19276069, PubMed:21336257, PubMed:23236473). Interacts with CPEB3 (via C-terminal RNA-binding region); recruits CNOT7 to CPEB3 to form a ternary complex required for mRNA deadenylation and decay (PubMed:21336257). Interacts with CNOT8 (PubMed:23236473). Interacts with CPEB4 (PubMed:21336257).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CNOT1A5YKK64EBI-723281,EBI-1222758
CNOT3O751752EBI-723281,EBI-743073
CNOT6LQ96LI53EBI-723281,EBI-1046635
CNOT7Q9UIV16EBI-723281,EBI-2105113
CPEB3Q8NE357EBI-723281,EBI-8596191
CPEB4Q17RY02EBI-723281,EBI-2848203
PABPC1P119404EBI-723281,EBI-81531

GO - Molecular functioni

  • receptor tyrosine kinase binding Source: UniProtKB
  • SH3/SH2 adaptor activity Source: ProtInc

Protein-protein interaction databases

BioGridi115443. 38 interactors.
IntActiP50616. 25 interactors.
MINTiMINT-1384463.
STRINGi9606.ENSP00000268957.

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 13Combined sources12
Turni14 – 19Combined sources6
Helixi22 – 40Combined sources19
Turni49 – 52Combined sources4
Helixi53 – 56Combined sources4
Beta strandi58 – 64Combined sources7
Helixi66 – 73Combined sources8
Turni74 – 76Combined sources3
Helixi79 – 85Combined sources7
Beta strandi90 – 95Combined sources6
Beta strandi98 – 104Combined sources7
Beta strandi109 – 114Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D5RX-ray2.50B1-115[»]
2Z15X-ray2.30A/B/C/D1-117[»]
5CI8X-ray2.33A1-345[»]
5CI9X-ray2.30A1-345[»]
ProteinModelPortaliP50616.
SMRiP50616.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50616.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 92Important for nuclear localizationBy similarityAdd BLAST11
Regioni161 – 218Required for interaction with CPEB31 PublicationAdd BLAST58

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi22 – 39Bipartite nuclear localization signalBy similarityAdd BLAST18
Motifi226 – 234Nuclear export signalBy similarity9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi237 – 245Poly-Gln9
Compositional biasi249 – 256Poly-Pro8
Compositional biasi257 – 262Poly-Gln6

Sequence similaritiesi

Belongs to the BTG family.Curated

Phylogenomic databases

eggNOGiKOG4006. Eukaryota.
ENOG410ZZC0. LUCA.
GeneTreeiENSGT00550000074461.
HOGENOMiHOG000253939.
HOVERGENiHBG006617.
InParanoidiP50616.
KOiK14443.
OMAiSIFPGDS.
OrthoDBiEOG091G0LEQ.
PhylomeDBiP50616.
TreeFamiTF105274.

Family and domain databases

InterProiIPR002087. Anti_prolifrtn.
IPR015677. Tob1.
IPR015676. Tob1/2.
[Graphical view]
PANTHERiPTHR17537. PTHR17537. 1 hit.
PTHR17537:SF6. PTHR17537:SF6. 1 hit.
PfamiPF07742. BTG. 1 hit.
[Graphical view]
PRINTSiPR00310. ANTIPRLFBTG1.
SMARTiSM00099. btg1. 1 hit.
[Graphical view]
SUPFAMiSSF160696. SSF160696. 1 hit.
PROSITEiPS00960. BTG_1. 1 hit.
PS01203. BTG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50616-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLEIQVALN FIISYLYNKL PRRRVNIFGE ELERLLKKKY EGHWYPEKPY
60 70 80 90 100
KGSGFRCIHI GEKVDPVIEQ ASKESGLDID DVRGNLPQDL SVWIDPFEVS
110 120 130 140 150
YQIGEKGPVK VLYVDDNNEN GCELDKEIKN SFNPEAQVFM PISDPASSVS
160 170 180 190 200
SSPSPPFGHS AAVSPTFMPR STQPLTFTTA TFAATKFGST KMKNSGRSNK
210 220 230 240 250
VARTSPINLG LNVNDLLKQK AISSSMHSLY GLGLGSQQQP QQQQQPAQPP
260 270 280 290 300
PPPPPPQQQQ QQKTSALSPN AKEFIFPNMQ GQGSSTNGMF PGDSPLNLSP
310 320 330 340
LQYSNAFDVF AAYGGLNEKS FVDGLNFSLN NMQYSNQQFQ PVMAN
Length:345
Mass (Da):38,155
Last modified:October 1, 1996 - v1
Checksum:i3C8AC51B49E1E69F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037840319K → R.Corresponds to variant rs3316dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38305 mRNA. Translation: BAA07423.1.
CR536487 mRNA. Translation: CAG38726.1.
CR541686 mRNA. Translation: CAG46487.1.
BT019380 mRNA. Translation: AAV38187.1.
BT019381 mRNA. Translation: AAV38188.1.
AK313904 mRNA. Translation: BAG36627.1.
CH471109 Genomic DNA. Translation: EAW94577.1.
CH471109 Genomic DNA. Translation: EAW94578.1.
BC031406 mRNA. Translation: AAH31406.1.
BC070493 mRNA. Translation: AAH70493.1.
BC098415 mRNA. Translation: AAH98415.1.
CCDSiCCDS11576.1.
RefSeqiNP_001230806.1. NM_001243877.1.
NP_001230814.1. NM_001243885.1.
NP_005740.1. NM_005749.3.
UniGeneiHs.703321.
Hs.744946.

Genome annotation databases

EnsembliENST00000268957; ENSP00000268957; ENSG00000141232.
ENST00000499247; ENSP00000427695; ENSG00000141232.
GeneIDi10140.
KEGGihsa:10140.
UCSCiuc002isw.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38305 mRNA. Translation: BAA07423.1.
CR536487 mRNA. Translation: CAG38726.1.
CR541686 mRNA. Translation: CAG46487.1.
BT019380 mRNA. Translation: AAV38187.1.
BT019381 mRNA. Translation: AAV38188.1.
AK313904 mRNA. Translation: BAG36627.1.
CH471109 Genomic DNA. Translation: EAW94577.1.
CH471109 Genomic DNA. Translation: EAW94578.1.
BC031406 mRNA. Translation: AAH31406.1.
BC070493 mRNA. Translation: AAH70493.1.
BC098415 mRNA. Translation: AAH98415.1.
CCDSiCCDS11576.1.
RefSeqiNP_001230806.1. NM_001243877.1.
NP_001230814.1. NM_001243885.1.
NP_005740.1. NM_005749.3.
UniGeneiHs.703321.
Hs.744946.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D5RX-ray2.50B1-115[»]
2Z15X-ray2.30A/B/C/D1-117[»]
5CI8X-ray2.33A1-345[»]
5CI9X-ray2.30A1-345[»]
ProteinModelPortaliP50616.
SMRiP50616.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115443. 38 interactors.
IntActiP50616. 25 interactors.
MINTiMINT-1384463.
STRINGi9606.ENSP00000268957.

PTM databases

iPTMnetiP50616.
PhosphoSitePlusiP50616.

Polymorphism and mutation databases

BioMutaiTOB1.
DMDMi1729989.

Proteomic databases

MaxQBiP50616.
PaxDbiP50616.
PeptideAtlasiP50616.
PRIDEiP50616.

Protocols and materials databases

DNASUi10140.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268957; ENSP00000268957; ENSG00000141232.
ENST00000499247; ENSP00000427695; ENSG00000141232.
GeneIDi10140.
KEGGihsa:10140.
UCSCiuc002isw.4. human.

Organism-specific databases

CTDi10140.
DisGeNETi10140.
GeneCardsiTOB1.
H-InvDBHIX0039399.
HGNCiHGNC:11979. TOB1.
HPAiCAB010908.
HPA047839.
MIMi605523. gene.
neXtProtiNX_P50616.
OpenTargetsiENSG00000141232.
PharmGKBiPA36663.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4006. Eukaryota.
ENOG410ZZC0. LUCA.
GeneTreeiENSGT00550000074461.
HOGENOMiHOG000253939.
HOVERGENiHBG006617.
InParanoidiP50616.
KOiK14443.
OMAiSIFPGDS.
OrthoDBiEOG091G0LEQ.
PhylomeDBiP50616.
TreeFamiTF105274.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000141232-MONOMER.
SignaLinkiP50616.
SIGNORiP50616.

Miscellaneous databases

ChiTaRSiTOB1. human.
EvolutionaryTraceiP50616.
GeneWikiiTOB1.
GenomeRNAii10140.
PROiP50616.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141232.
CleanExiHS_TOB1.
GenevisibleiP50616. HS.

Family and domain databases

InterProiIPR002087. Anti_prolifrtn.
IPR015677. Tob1.
IPR015676. Tob1/2.
[Graphical view]
PANTHERiPTHR17537. PTHR17537. 1 hit.
PTHR17537:SF6. PTHR17537:SF6. 1 hit.
PfamiPF07742. BTG. 1 hit.
[Graphical view]
PRINTSiPR00310. ANTIPRLFBTG1.
SMARTiSM00099. btg1. 1 hit.
[Graphical view]
SUPFAMiSSF160696. SSF160696. 1 hit.
PROSITEiPS00960. BTG_1. 1 hit.
PS01203. BTG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOB1_HUMAN
AccessioniPrimary (citable) accession number: P50616
Secondary accession number(s): B2R9T0, D3DTY3, Q4KMQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.