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Protein

Methionine aminopeptidase

Gene

map

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Metal bindingi94Divalent metal cation 1UniRule annotation1
Metal bindingi105Divalent metal cation 1UniRule annotation1
Metal bindingi105Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi168Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei175SubstrateUniRule annotation1
Metal bindingi201Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi232Divalent metal cation 1UniRule annotation1
Metal bindingi232Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:CPE2383
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489361 – 249Methionine aminopeptidaseAdd BLAST249

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP50614
SMRiP50614
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000030426
KOiK01265
OMAiSYFHGPP
OrthoDBiPOG091H01DX

Family and domain databases

CDDicd01086 MetAP1, 1 hit
HAMAPiMF_01974 MetAP_1, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002467 Pept_M24A_MAP1
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00500 met_pdase_I, 1 hit
PROSITEiView protein in PROSITE
PS00680 MAP_1, 1 hit

Sequencei

Sequence statusi: Complete.

P50614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIILKNENEI DLMRKAGKIL GETLNLLKEK VRPGITTTEL DRIAEEFITK
60 70 80 90 100
HGATPSFKGL YGFPASLCIS VNNQVIHGFP GSYELKDGDI VSIDCGVCLN
110 120 130 140 150
GFHSDAARTF GVGNISEEAE KLIRITEESF FKGIEKAYVD NRLTDISNEI
160 170 180 190 200
QQYVEANGFS VVRDFVGHGI GRKVHEDPEV PNFGRPGRGP KLMAGMVLAI
210 220 230 240
EPMVNMGSYR VKTLDDGWTV VTADGKLSAH YENTVAILPN GPEILTLIK
Length:249
Mass (Da):27,323
Last modified:January 31, 2002 - v2
Checksum:i7FDC5CD82CEAFAC7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30K → Q in CAA60211 (PubMed:7559358).Curated1
Sequence conflicti104S → R in CAA60211 (PubMed:7559358).Curated1
Sequence conflicti148N → K in CAA60211 (PubMed:7559358).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA Translation: BAB82089.1
X86486 Genomic DNA Translation: CAA60211.1
RefSeqiWP_003454388.1, NC_003366.1

Genome annotation databases

EnsemblBacteriaiBAB82089; BAB82089; BAB82089
GeneIDi29570199
KEGGicpe:CPE2383

Similar proteinsi

Entry informationi

Entry nameiMAP1_CLOPE
AccessioniPrimary (citable) accession number: P50614
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 31, 2002
Last modified: March 28, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health