Flagellar hook protein FlgE - Helicobacter pylori (strain ATCC 700392 / 26695)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Flagellar hook protein FlgE

Gene names

Name:	flgE
Ordered Locus Names:	HP_0870

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter ›

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	A flexible structure which links the flagellar filament to the drive apparatus in the basal body. Absence of the gene leads to absence of the hook protein, lack of the flagellar filament and thus loss of mobility. Approximately wild-type levels of the flagellar subunits are still produced and accumulate mostly in the cytosol.
Subcellular location	Bacterial flagellum basal body.
Miscellaneous	The N-terminal amino acid sequence was from strain CCUG 951; the DNA sequence was from strain CCUG 17874. Disruption and subcellular localization experiments were performed for both strains. The apparent pI for the hook protein was 5.1.
Sequence similarities	Belongs to the flagella basal body rod proteins family.

Ontologies

Keywords
Cellular component	Bacterial flagellum
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ciliary or flagellar motility Inferred from electronic annotation. Source: InterPro flagellum organization Inferred from electronic annotation. Source: InterPro
Cellular_component	bacterial-type flagellum basal body, rod Inferred from electronic annotation. Source: InterPro
Molecular_function	motor activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 718

718

Flagellar hook protein FlgE

PRO_0000180828

Experimental info

Sequence conflict

106

1

S → N in AAA92803. Ref.1

Sequence conflict

237

1

C → Y in AAA92803. Ref.1

Sequence conflict

259

1

A → P in AAA92803. Ref.1

Sequence conflict

373 – 374

2

DI → NS in AAA92803. Ref.1

Sequence conflict

413 – 414

2

SV → TI in AAA92803. Ref.1

Sequence conflict

509

1

E → D in AAA92803. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P50610 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: F36EB60684AD1333
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FASTA

718

76,207

        10         20         30         40         50         60 
MLRSLWSGVN GMQAHQIALD IESNNIANVN TTGFKYSRAS FVDMLSQVKL IATAPYKNGL 

        70         80         90        100        110        120 
AGQNDFSVGL GVGVDATTKI FSQGNIQNTD VKTDLAIQGD GFFIISPDRG ITRNFTRDGE 

       130        140        150        160        170        180 
FLFDSQGSLV TTGGLVVQGW VRNGSDTGNK GSDTDALKVD NTGPLENIRI DPGMVMPARA 

       190        200        210        220        230        240 
SNRISMRANL NAGRHADQTA AIFALDSSAK TPSDGINPVY DSGTNLAQVA EDMGSLCNED 

       250        260        270        280        290        300 
GDALLLNENQ GIWVSYKSAK MVKDILPSAE NSTLELNGVK ISFTNDSAVS RTSSLVAAKN 

       310        320        330        340        350        360 
AINAVKSQTG IEAYLDGKQL RLENTNELDG DEKLKNIVVT QAGTGAFANF LDGDKDVTAF 

       370        380        390        400        410        420 
KYSYTHSISP NADIGQFRTT EDLRALIQHD ANIVKDPSLA DNYQDSAASI GVSVNQYGMF 

       430        440        450        460        470        480 
EINNKDNKNV IKENLNIFVS GYSSDSVTNN VLFKNAMKGL NTASLIEGGA SASSSKFTHA 

       490        500        510        520        530        540 
THATSIDVID SLGTKHAMRI EFYRSGGAEW NFRVIVPEPG ELVGGSAARP NVFEGGRLHF 

       550        560        570        580        590        600 
NNDGSLAGMN PPLLQFDPKN GADAPQRINL AFGSSGSFDG LTSVDKISET YAIEQNGYQA 

       610        620        630        640        650        660 
GDLMDVRFDS DGVLLGAFSN GRTLALAQVA LANFANDAGL QALGGNVFSQ TGNSGQALIG 

       670        680        690        700        710 
AANTGRRGSI SGSKLESSNV DLSRSLTNLI VVQRGFQANS KAVTTSDQIL NTLLNLKQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Non-motile mutants of Helicobacter pylori and Helicobacter mustelae defective in flagellar hook production." O'Toole P.W., Kostrzynska M., Trust T.J. Mol. Microbiol. 14:691-703(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-25, CHARACTERIZATION. Strain: CCUG 951 and DSM 4867 / CCUG 17874 / NCTC 11638.
[2]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09549 Genomic DNA. Translation: AAA92803.1. AE000511 Genomic DNA. Translation: AAD07911.1.
PIR	F64628.
RefSeq	NP_207664.1. NC_000915.1. YP_006934784.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P50610.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3456N.
MINT	MINT-162602.
STRING	85962.HP0870.
PTM databases
PhosSite	P11041018.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07911; AAD07911; HP_0870.
GeneID	13870054. 899399.
KEGG	heo:C694_04455. hpy:HP0870.
PATRIC	20593029. VBIHelPyl33062_0902.
Phylogenomic databases
eggNOG	COG1749.
KO	K02390.
OMA	GEFTNGK.
ProtClustDB	PRK08425.
Family and domain databases
InterPro	IPR011491. Falgellar_basal_body_FlaE. IPR001444. Flag_bb_rod_N. IPR020013. Flagellar_FlgE/F/G. IPR010810. Flagellin_hook_IN_motif. IPR010930. Flg_bb/hook_C_dom. IPR012835. FlgE_epsilon. [Graphical view]
Pfam	PF07559. FlaE. 1 hit. PF07196. Flagellin_IN. 1 hit. PF00460. Flg_bb_rod. 1 hit. PF06429. Flg_bbr_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR02489. flgE_epsilon. 1 hit. TIGR03506. FlgEFG_subfam. 2 hits.
PROSITE	PS00588. FLAGELLA_BB_ROD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLGE_HELPY

Accession

Primary (citable) accession number: P50610

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Helicobacter pylori

Helicobacter pylori (strain 26695): entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents