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Protein

Ribonuclease PH

Gene

rph

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates.By similarity

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PH (EC:2.7.7.56)
Short name:
RNase PH
Alternative name(s):
tRNA nucleotidyltransferase
Gene namesi
Name:rph
Ordered Locus Names:PA5334
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5334.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Ribonuclease PHPRO_0000139924Add
BLAST

Proteomic databases

PaxDbiP50597.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5334.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 206Combined sources
Beta strandi22 – 3312Combined sources
Beta strandi36 – 4712Combined sources
Beta strandi59 – 668Combined sources
Beta strandi70 – 734Combined sources
Helixi78 – 814Combined sources
Helixi86 – 10116Combined sources
Helixi105 – 1073Combined sources
Beta strandi110 – 12112Combined sources
Helixi126 – 14722Combined sources
Beta strandi160 – 1689Combined sources
Beta strandi171 – 1755Combined sources
Helixi178 – 1836Combined sources
Beta strandi185 – 1939Combined sources
Beta strandi198 – 20811Combined sources
Helixi212 – 23726Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R6LX-ray1.90A1-239[»]
1R6MX-ray2.00A1-239[»]
ProteinModelPortaliP50597.
SMRiP50597. Positions 1-239.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50597.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase PH family.Curated

Phylogenomic databases

eggNOGiENOG4105ED0. Bacteria.
COG0689. LUCA.
HOGENOMiHOG000229516.
InParanoidiP50597.
KOiK00989.
OMAiKGKGQGW.
OrthoDBiEOG6CZQQP.
PhylomeDBiP50597.

Family and domain databases

Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50597-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRPSGRAAD QLRPIRITRH YTKHAEGSVL VEFGDTKVIC TVSAESGVPR
60 70 80 90 100
FLKGQGQGWL TAEYGMLPRS TGERNQREAS RGKQGGRTLE IQRLIGRSLR
110 120 130 140 150
AALDLSKLGE NTLYIDCDVI QADGGTRTAS ITGATVALID ALAVLKKRGA
160 170 180 190 200
LKGNPLKQMV AAVSVGIYQG VPVLDLDYLE DSAAETDLNV VMTDAGGFIE
210 220 230
VQGTAEGAPF RPAELNAMLE LAQQGMQELF ELQRAALAE
Length:239
Mass (Da):25,654
Last modified:December 8, 2000 - v2
Checksum:i76F4717C31261EF7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681P → R in AAC44429 (PubMed:8824606).Curated
Sequence conflicti96 – 961G → D in AAC44429 (PubMed:8824606).Curated
Sequence conflicti122 – 1221A → T in AAC44429 (PubMed:8824606).Curated
Sequence conflicti137 – 1371A → S in AAC44429 (PubMed:8824606).Curated
Sequence conflicti149 – 1491G → V in AAC44429 (PubMed:8824606).Curated
Sequence conflicti159 – 1591M → I in AAC44429 (PubMed:8824606).Curated
Sequence conflicti186 – 1861T → S in AAC44429 (PubMed:8824606).Curated
Sequence conflicti195 – 1973AGG → CRR in AAC44429 (PubMed:8824606).Curated
Sequence conflicti230 – 2323FEL → VRT in AAC44429 (PubMed:8824606).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38241 Genomic DNA. Translation: AAC44429.1.
AE004091 Genomic DNA. Translation: AAG08719.1.
PIRiD82978.
RefSeqiNP_254021.1. NC_002516.2.
WP_003096595.1. NZ_ASJY01000822.1.

Genome annotation databases

EnsemblBacteriaiAAG08719; AAG08719; PA5334.
GeneIDi878218.
KEGGipae:PA5334.
PATRICi19845525. VBIPseAer58763_5589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38241 Genomic DNA. Translation: AAC44429.1.
AE004091 Genomic DNA. Translation: AAG08719.1.
PIRiD82978.
RefSeqiNP_254021.1. NC_002516.2.
WP_003096595.1. NZ_ASJY01000822.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R6LX-ray1.90A1-239[»]
1R6MX-ray2.00A1-239[»]
ProteinModelPortaliP50597.
SMRiP50597. Positions 1-239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5334.

Proteomic databases

PaxDbiP50597.

Protocols and materials databases

DNASUi878218.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08719; AAG08719; PA5334.
GeneIDi878218.
KEGGipae:PA5334.
PATRICi19845525. VBIPseAer58763_5589.

Organism-specific databases

PseudoCAPiPA5334.

Phylogenomic databases

eggNOGiENOG4105ED0. Bacteria.
COG0689. LUCA.
HOGENOMiHOG000229516.
InParanoidiP50597.
KOiK00989.
OMAiKGKGQGW.
OrthoDBiEOG6CZQQP.
PhylomeDBiP50597.

Miscellaneous databases

EvolutionaryTraceiP50597.

Family and domain databases

Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product."
    Macgregor C.H., Arora S.K., Hager P.W., Dail M.B., Phibbs P.V. Jr.
    J. Bacteriol. 178:5627-5635(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiRNPH_PSEAE
AccessioniPrimary (citable) accession number: P50597
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 8, 2000
Last modified: November 11, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.