Skip Header

Contribute Send feedback
Read comments (?) or add your own

P50597 (RNPH_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56
Alternative name(s):
tRNA nucleotidyltransferase
Gene names
Name:rph
Ordered Locus Names:PA5334
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564

Catalytic activity

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564

Sequence similarities

Belongs to the RNase PH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 239239Ribonuclease PH HAMAP MF_00564
PRO_0000139924

Experimental info

Sequence conflict681P → R in AAC44429. Ref.1
Sequence conflict961G → D in AAC44429. Ref.1
Sequence conflict1221A → T in AAC44429. Ref.1
Sequence conflict1371A → S in AAC44429. Ref.1
Sequence conflict1491G → V in AAC44429. Ref.1
Sequence conflict1591M → I in AAC44429. Ref.1
Sequence conflict1861T → S in AAC44429. Ref.1
Sequence conflict195 – 1973AGG → CRR in AAC44429. Ref.1
Sequence conflict230 – 2323FEL → VRT in AAC44429. Ref.1

Secondary structure

1 ................................. 239
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P50597 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: 76F4717C31261EF7

FASTA23925,654
        10         20         30         40         50         60 
MNRPSGRAAD QLRPIRITRH YTKHAEGSVL VEFGDTKVIC TVSAESGVPR FLKGQGQGWL 

        70         80         90        100        110        120 
TAEYGMLPRS TGERNQREAS RGKQGGRTLE IQRLIGRSLR AALDLSKLGE NTLYIDCDVI 

       130        140        150        160        170        180 
QADGGTRTAS ITGATVALID ALAVLKKRGA LKGNPLKQMV AAVSVGIYQG VPVLDLDYLE 

       190        200        210        220        230 
DSAAETDLNV VMTDAGGFIE VQGTAEGAPF RPAELNAMLE LAQQGMQELF ELQRAALAE

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product."
Macgregor C.H., Arora S.K., Hager P.W., Dail M.B., Phibbs P.V. Jr.
J. Bacteriol. 178:5627-5635(1996) [PubMed: 8824606] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed: 10984043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U38241 Genomic DNA. Translation: AAC44429.1.
AE004091 Genomic DNA. Translation: AAG08719.1.
PIRD82978.
RefSeqNP_254021.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1R6LX-ray1.90A1-239[»]
1R6MX-ray2.00A1-239[»]
ProteinModelPortalP50597.
SMRP50597. Positions 1-239.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID878218.
GenomeReviewsGene locus PA5334 in contig AE004091_GR.
KEGGpae:PA5334.
PATRIC19845525. VBIPseAer58763_5589.

Organism-specific databases

PseudoCAPPA5334.

Phylogenomic databases

HOGENOMHBG737187.
OMAMLPRATG.
ProtClustDBPRK00173.

Enzyme and pathway databases

BioCycPAER208964:PA5334-MONOMER.

Family and domain databases

HAMAPMF_00564. RNase_PH.
[Tree]
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
KOK00989.
PfamPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMSSF55666. 3_ExoRNase. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMsTIGR01966. RNasePH. 1 hit.
PROSITEPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNPH_PSEAE
AccessionPrimary (citable) accession number: P50597
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 8, 2000
Last modified: January 25, 2012
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents