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Protein

Ribonuclease PH

Gene

rph

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates.By similarity

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PH (EC:2.7.7.56)
Short name:
RNase PH
Alternative name(s):
tRNA nucleotidyltransferase
Gene namesi
Name:rph
Ordered Locus Names:PA5334
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5334.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001399241 – 239Ribonuclease PHAdd BLAST239

Proteomic databases

PaxDbiP50597.
PRIDEiP50597.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5334.

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 20Combined sources6
Beta strandi22 – 33Combined sources12
Beta strandi36 – 47Combined sources12
Beta strandi59 – 66Combined sources8
Beta strandi70 – 73Combined sources4
Helixi78 – 81Combined sources4
Helixi86 – 101Combined sources16
Helixi105 – 107Combined sources3
Beta strandi110 – 121Combined sources12
Helixi126 – 147Combined sources22
Beta strandi160 – 168Combined sources9
Beta strandi171 – 175Combined sources5
Helixi178 – 183Combined sources6
Beta strandi185 – 193Combined sources9
Beta strandi198 – 208Combined sources11
Helixi212 – 237Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R6LX-ray1.90A1-239[»]
1R6MX-ray2.00A1-239[»]
ProteinModelPortaliP50597.
SMRiP50597.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50597.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase PH family.Curated

Phylogenomic databases

eggNOGiENOG4105ED0. Bacteria.
COG0689. LUCA.
HOGENOMiHOG000229516.
InParanoidiP50597.
KOiK00989.
OMAiKGKGQGW.
PhylomeDBiP50597.

Family and domain databases

CDDicd11362. RNase_PH_bact. 1 hit.
Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P50597-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRPSGRAAD QLRPIRITRH YTKHAEGSVL VEFGDTKVIC TVSAESGVPR
60 70 80 90 100
FLKGQGQGWL TAEYGMLPRS TGERNQREAS RGKQGGRTLE IQRLIGRSLR
110 120 130 140 150
AALDLSKLGE NTLYIDCDVI QADGGTRTAS ITGATVALID ALAVLKKRGA
160 170 180 190 200
LKGNPLKQMV AAVSVGIYQG VPVLDLDYLE DSAAETDLNV VMTDAGGFIE
210 220 230
VQGTAEGAPF RPAELNAMLE LAQQGMQELF ELQRAALAE
Length:239
Mass (Da):25,654
Last modified:December 8, 2000 - v2
Checksum:i76F4717C31261EF7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68P → R in AAC44429 (PubMed:8824606).Curated1
Sequence conflicti96G → D in AAC44429 (PubMed:8824606).Curated1
Sequence conflicti122A → T in AAC44429 (PubMed:8824606).Curated1
Sequence conflicti137A → S in AAC44429 (PubMed:8824606).Curated1
Sequence conflicti149G → V in AAC44429 (PubMed:8824606).Curated1
Sequence conflicti159M → I in AAC44429 (PubMed:8824606).Curated1
Sequence conflicti186T → S in AAC44429 (PubMed:8824606).Curated1
Sequence conflicti195 – 197AGG → CRR in AAC44429 (PubMed:8824606).Curated3
Sequence conflicti230 – 232FEL → VRT in AAC44429 (PubMed:8824606).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38241 Genomic DNA. Translation: AAC44429.1.
AE004091 Genomic DNA. Translation: AAG08719.1.
PIRiD82978.
RefSeqiNP_254021.1. NC_002516.2.
WP_003096595.1. NZ_ASJY01000822.1.

Genome annotation databases

EnsemblBacteriaiAAG08719; AAG08719; PA5334.
GeneIDi878218.
KEGGipae:PA5334.
PATRICi19845525. VBIPseAer58763_5589.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38241 Genomic DNA. Translation: AAC44429.1.
AE004091 Genomic DNA. Translation: AAG08719.1.
PIRiD82978.
RefSeqiNP_254021.1. NC_002516.2.
WP_003096595.1. NZ_ASJY01000822.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R6LX-ray1.90A1-239[»]
1R6MX-ray2.00A1-239[»]
ProteinModelPortaliP50597.
SMRiP50597.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5334.

Proteomic databases

PaxDbiP50597.
PRIDEiP50597.

Protocols and materials databases

DNASUi878218.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08719; AAG08719; PA5334.
GeneIDi878218.
KEGGipae:PA5334.
PATRICi19845525. VBIPseAer58763_5589.

Organism-specific databases

PseudoCAPiPA5334.

Phylogenomic databases

eggNOGiENOG4105ED0. Bacteria.
COG0689. LUCA.
HOGENOMiHOG000229516.
InParanoidiP50597.
KOiK00989.
OMAiKGKGQGW.
PhylomeDBiP50597.

Miscellaneous databases

EvolutionaryTraceiP50597.

Family and domain databases

CDDicd11362. RNase_PH_bact. 1 hit.
Gene3Di3.30.230.70. 1 hit.
HAMAPiMF_00564. RNase_PH. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR002381. RNase_PH_bac-type.
IPR018336. RNase_PH_CS.
[Graphical view]
PfamiPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsiTIGR01966. RNasePH. 1 hit.
PROSITEiPS01277. RIBONUCLEASE_PH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNPH_PSEAE
AccessioniPrimary (citable) accession number: P50597
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 8, 2000
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.