ID   LEP_RAT                 Reviewed;         167 AA.
AC   P50596;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-NOV-2011, entry version 86.
DE   RecName: Full=Leptin;
DE   AltName: Full=Obesity factor;
DE   Flags: Precursor;
GN   Name=Lep; Synonyms=Ob;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   MEDLINE=95386724; PubMed=7657834; DOI=10.1172/JCI118204;
RA   Ogawa Y., Masuzaki H., Isse N., Okazaki T., Mori K., Shigemoto M.,
RA   Satoh N., Tamura N., Hosoda K., Yoshimasa Y., Jingami H., Kawada T.,
RA   Nakao K.;
RT   "Molecular cloning of rat obese cDNA and augmented gene expression in
RT   genetically obese Zucker fatty (fa/fa) rats.";
RL   J. Clin. Invest. 96:1647-1652(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Adipose tissue;
RX   MEDLINE=95314614; PubMed=7794258; DOI=10.1006/bbrc.1995.1837;
RA   Funahashi T., Shimomura I., Hiraoka H., Arai T., Takahashi M.,
RA   Nakamura T., Nozaki S., Yamashita S., Takemura K., Tokunaga K.;
RT   "Enhanced expression of rat obese (ob) gene in adipose tissues of
RT   ventromedial hypothalamus (VMH)-lesioned rats.";
RL   Biochem. Biophys. Res. Commun. 211:469-475(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Leto, OLETF, and Zucker; TISSUE=Adipose tissue;
RX   MEDLINE=95251725; PubMed=7733988; DOI=10.1006/bbrc.1995.1589;
RA   Murakami T., Shima K.;
RT   "Cloning of rat obese cDNA and its expression in obese rats.";
RL   Biochem. Biophys. Res. Commun. 209:944-952(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 14-167.
RC   STRAIN=Sprague-Dawley; TISSUE=Adipose tissue;
RA   Donohoue P.A., Sivitz W.I., Bailey H.L.;
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May function as part of a signaling pathway that acts to
CC       regulate the size of the body fat depot. An increase in the level
CC       of Lep may act directly or indirectly on the CNS to inhibit food
CC       intake and/or regulate energy expenditure as part of a homeostatic
CC       mechanism to maintain constancy of the adipose mass.
CC   -!- SUBCELLULAR LOCATION: Secreted (Probable).
CC   -!- SIMILARITY: Belongs to the leptin family.
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DR   EMBL; D45862; BAA08296.1; -; mRNA.
DR   EMBL; S78586; AAB34657.2; -; mRNA.
DR   EMBL; D49653; BAA08529.1; -; mRNA.
DR   EMBL; U48849; AAC52514.1; -; mRNA.
DR   IPI; IPI00199521; -.
DR   PIR; PC4034; LTRT.
DR   RefSeq; NP_037208.1; NM_013076.3.
DR   UniGene; Rn.44444; -.
DR   ProteinModelPortal; P50596; -.
DR   SMR; P50596; 24-167.
DR   STRING; P50596; -.
DR   Ensembl; ENSRNOT00000008020; ENSRNOP00000008020; ENSRNOG00000006059.
DR   GeneID; 25608; -.
DR   KEGG; rno:25608; -.
DR   UCSC; D49653; rat.
DR   CTD; 3952; -.
DR   RGD; 3000; Lep.
DR   eggNOG; roNOG17007; -.
DR   GeneTree; ENSGT00390000011772; -.
DR   HOVERGEN; HBG007860; -.
DR   InParanoid; P50596; -.
DR   OMA; QISNDLE; -.
DR   OrthoDB; EOG4QNMXC; -.
DR   PhylomeDB; P50596; -.
DR   NextBio; 607339; -.
DR   ArrayExpress; P50596; -.
DR   Genevestigator; P50596; -.
DR   GermOnline; ENSRNOG00000006059; Rattus norvegicus.
DR   GO; GO:0005615; C:extracellular space; IDA:HGNC.
DR   GO; GO:0005622; C:intracellular; IDA:GOC.
DR   GO; GO:0005125; F:cytokine activity; TAS:RGD.
DR   GO; GO:0005179; F:hormone activity; IDA:RGD.
DR   GO; GO:0051428; F:peptide hormone receptor binding; IDA:RGD.
DR   GO; GO:0008343; P:adult feeding behavior; ISS:HGNC.
DR   GO; GO:0035630; P:bone mineralization involved in bone maturation; IDA:RGD.
DR   GO; GO:0019933; P:cAMP-mediated signaling; TAS:RGD.
DR   GO; GO:0071298; P:cellular response to L-ascorbic acid; IEP:RGD.
DR   GO; GO:0071300; P:cellular response to retinoic acid; IEP:RGD.
DR   GO; GO:0007623; P:circadian rhythm; IEP:RGD.
DR   GO; GO:0006112; P:energy reserve metabolic process; IDA:RGD.
DR   GO; GO:0009062; P:fatty acid catabolic process; IDA:RGD.
DR   GO; GO:0007565; P:female pregnancy; IEP:RGD.
DR   GO; GO:0006114; P:glycerol biosynthetic process; IDA:RGD.
DR   GO; GO:0007259; P:JAK-STAT cascade; TAS:RGD.
DR   GO; GO:0033210; P:leptin-mediated signaling pathway; IDA:RGD.
DR   GO; GO:0050901; P:leukocyte tethering or rolling; IDA:RGD.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; IDA:RGD.
DR   GO; GO:0032099; P:negative regulation of appetite; ISS:HGNC.
DR   GO; GO:0061037; P:negative regulation of cartilage development; IDA:RGD.
DR   GO; GO:2000486; P:negative regulation of glutamine transport; IMP:RGD.
DR   GO; GO:0045906; P:negative regulation of vasoconstriction; IDA:RGD.
DR   GO; GO:0001542; P:ovulation from ovarian follicle; IDA:RGD.
DR   GO; GO:0008284; P:positive regulation of cell proliferation; IDA:RGD.
DR   GO; GO:0001819; P:positive regulation of cytokine production; IEP:RGD.
DR   GO; GO:0046881; P:positive regulation of follicle-stimulating hormone secretion; IEP:RGD.
DR   GO; GO:2000491; P:positive regulation of hepatic stellate cell activation; IDA:RGD.
DR   GO; GO:0046628; P:positive regulation of insulin receptor signaling pathway; IDA:RGD.
DR   GO; GO:0043270; P:positive regulation of ion transport; IDA:RGD.
DR   GO; GO:0033686; P:positive regulation of luteinizing hormone secretion; IEP:RGD.
DR   GO; GO:0043410; P:positive regulation of MAPKKK cascade; IDA:RGD.
DR   GO; GO:0042517; P:positive regulation of tyrosine phosphorylation of Stat3 protein; IMP:RGD.
DR   GO; GO:0008217; P:regulation of blood pressure; IDA:RGD.
DR   GO; GO:0060587; P:regulation of lipoprotein lipid oxidation; IDA:RGD.
DR   GO; GO:0002021; P:response to dietary excess; IMP:RGD.
DR   GO; GO:0001666; P:response to hypoxia; IEP:RGD.
DR   GO; GO:0033197; P:response to vitamin E; IEP:RGD.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR012351; 4_helix_cytokine_core.
DR   InterPro; IPR000065; Leptin.
DR   Gene3D; G3DSA:1.20.1250.10; 4_helix_cytokine_core; 1.
DR   KO; K05424; -.
DR   PANTHER; PTHR11724; Leptin; 1.
DR   Pfam; PF02024; Leptin; 1.
DR   PIRSF; PIRSF001837; Leptin; 1.
DR   PRINTS; PR00495; LEPTIN.
DR   ProDom; PD005698; Leptin; 1.
DR   SUPFAM; SSF47266; 4_helix_cytokine; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Disulfide bond; Obesity; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL        1     21       Potential.
FT   CHAIN        22    167       Leptin.
FT                                /FTId=PRO_0000017690.
FT   DISULFID    117    167       By similarity.
FT   CONFLICT     32     32       K -> T (in Ref. 2).
FT   CONFLICT    163    163       L -> V (in Ref. 4; AAC52514).
SQ   SEQUENCE   167 AA;  18866 MW;  3B5B563DA42EC84E CRC64;
     MCWRPLCRFL WLWSYLSYVQ AVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL
     DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP
     QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC
//