Reviewed, UniProtKB/Swiss-Prot P50596 (LEP_RAT)

Names and origin

Protein names

Recommended name:
    Leptin
Alternative name(s):
    Obesity factor

Gene names

Name:	Lep
Synonyms:	Ob

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of Lep may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Subcellular location	SecretedProbable.
Sequence similarities	Belongs to the leptin family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Obesity
Domain	Signal
Gene Ontology (GO)
Biological process	adult feeding behavior Inferred from sequence or structural similarity. Source: HGNC negative regulation of appetite Inferred from sequence or structural similarity. Source: HGNC signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from direct assay. Source: HGNC
Molecular function	hormone activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 167	146	Leptin		PRO_0000017690
Amino acid modifications
	Disulfide bond	117 ↔ 167		By similarity
Experimental info
	Sequence conflict	32	1	K → T Ref.2
	Sequence conflict	163	1	L → V in AAC52514. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P50596-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3B5B563DA42EC84E	FASTA	167	18,866
	10 20 30 40 50 60 MCWRPLCRFL WLWSYLSYVQ AVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL 70 80 90 100 110 120 DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP 130 140 150 160 QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC

References

[1]	"Molecular cloning of rat obese cDNA and augmented gene expression in genetically obese Zucker fatty (fa/fa) rats." Ogawa Y., Masuzaki H., Isse N., Okazaki T., Mori K., Shigemoto M., Satoh N., Tamura N., Hosoda K., Yoshimasa Y., Jingami H., Kawada T., Nakao K. J. Clin. Invest. 96:1647-1652(1995) [PubMed: 7657834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"Enhanced expression of rat obese (ob) gene in adipose tissues of ventromedial hypothalamus (VMH)-lesioned rats." Funahashi T., Shimomura I., Hiraoka H., Arai T., Takahashi M., Nakamura T., Nozaki S., Yamashita S., Takemura K., Tokunaga K. Biochem. Biophys. Res. Commun. 211:469-475(1995) [PubMed: 7794258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Adipose tissue.
[3]	"Cloning of rat obese cDNA and its expression in obese rats." Murakami T., Shima K. Biochem. Biophys. Res. Commun. 209:944-952(1995) [PubMed: 7733988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Leto, OLETF and Zucker. Tissue: Adipose tissue.
[4]	Donohoue P.A., Sivitz W.I., Bailey H.L. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-167. Strain: Sprague-Dawley. Tissue: Adipose tissue.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D45862 mRNA. Translation: BAA08296.1. S78586 mRNA. Translation: AAB34657.2. D49653 mRNA. Translation: BAA08529.1. U48849 mRNA. Translation: AAC52514.1.
PIR	LTRT. PC4034.
RefSeq	NP_037208.1.
UniGene	Rn.44444
3D structure databases
HSSP	HSSP built from PDB template 1AX8 based on UniProtKB P41159.
SMR	P50596. Positions 24-167.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000006059. Rattus norvegicus. [Contig view]
GeneID	25608.
KEGG	rno:25608.
Organism-specific databases
RGD	3000. Lep.
Phylogenomic databases
HOVERGEN	P50596.
Gene expression databases
ArrayExpress	P50596.
GermOnline	ENSRNOG00000006059. Rattus norvegicus.
Family and domain databases
InterPro	IPR012351. 4_helix_cytokine_core. IPR000065. Leptin. [Graphical view]
Gene3D	G3DSA:1.20.1250.10. 4_helix_cytokine_core. 1 hit.
PANTHER	PTHR11724. Leptin. 1 hit.
Pfam	PF02024. Leptin. 1 hit. [Graphical view]
PIRSF	PIRSF001837. Leptin. 1 hit.
PRINTS	PR00495. LEPTIN.
ProDom	PD005698. Leptin. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	607339.

Entry information

Entry name

LEP_RAT

Accession

Primary (citable) accession number: P50596

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)