P50595 (LEP_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leptin

Alternative name(s):
Obesity factor

Gene names

Name:	LEP
Synonyms:	OB, OBS

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the leptin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Disease	Obesity
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adult feeding behavior Inferred from sequence or structural similarity. Source: HGNC negative regulation of appetite Inferred from sequence or structural similarity. Source: HGNC signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular space Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 167	146	Leptin		PRO_0000017679
Amino acid modifications
	Disulfide bond	117 ↔ 167		By similarity
Natural variations
	Natural variant	25	1	R → C. Ref.2 Ref.3 Ref.4
	Natural variant	80	1	A → V. Ref.2 Ref.3
Experimental info
	Sequence conflict	25	1	R → Q in AAB06579. Ref.6
	Sequence conflict	166	1	G → E in AAB06579. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P50595 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 94C666B3069E50B7	FASTA	167	18,716
	10 20 30 40 50 60 MRCGPLYRFL WLWPYLSYVE AVPIRKVQDD TKTLIKTIVT RINDISHTQS VSSKQRVTGL 70 80 90 100 110 120 DFIPGLHPLL SLSKMDQTLA IYQQILTSLP SRNVVQISND LENLRDLLHL LAASKSCPLP 130 140 150 160 QVRALESLES LGVVLEASLY STEVVALSRL QGSLQDMLRQ LDLSPGC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Tellam R.L., Briscoe S., Vuocolo A. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Liver.
[2]	"Resequencing of DNA from a diverse panel of cattle reveals a high level of polymorphism in both intron and exon." Konfortov B.A., Licence V.E., Miller J.R. Mamm. Genome 10:1142-1145(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-25 AND VAL-80. Strain: Jersey.
[3]	"Association of leptin gene polymorphisms with serum leptin concentration in dairy cows." Liefers S.C., te Pas M.F.W., Veerkamp R.F., Chilliard Y., Delavaud C., Gerritsen R., van der Lende T. Mamm. Genome 14:657-663(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-25 AND VAL-80.
[4]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT CYS-25.
[5]	"Exon two of the bovine obese, leptin, gene." Fitzsimmons C.J., Schmutz S.M. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
[6]	"Partial cloning of bovine obesity gene." Ji S., Spurlock M.E. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-167.
[7]	Kawakita Y., Abe H., Miyashige T. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 22-167. Tissue: White adipose tissue.
[8]	"Two novel polymorphisms in the bovine obesity gene (OBS)." Lien S., Sundvold H., Klungland H., Vaege D.I. Anim. Genet. 28:245-245(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-145.
[9]	"Chromosomal localization of the bovine obesity (OBS) gene." Pfister-Genskow M.A., Hayes H., Eggen A., Bishop M.D. Mamm. Genome 7:398-399(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-146.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U43943 mRNA. Translation: AAA85906.1.
U50365 Genomic DNA. Translation: AAB61244.1.
AJ236854 Genomic DNA. Translation: CAB38018.1.
AJ132764 Genomic DNA. Translation: CAB64255.1.
AJ512638, AJ512639 Genomic DNA. Translation: CAD54745.1.
AF120500 Genomic DNA. Translation: AAD23567.1.
BT020625 mRNA. Translation: AAX08642.1.
U65793 mRNA. Translation: AAB06579.1.
AB003143 mRNA. Translation: BAA19750.1.
Y11369 Genomic DNA. Translation: CAA72197.1.
U43833 Genomic DNA. Translation: AAB18762.1.

IPI

IPI00693836.

RefSeq

NP_776353.2. NM_173928.2.

UniGene

Bt.1939.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A16	model	-	A	1-167	[»]

ProteinModelPortal

P50595.

SMR

P50595. Positions 24-167.

ModBase

Search...

Proteomic databases

PRIDE

P50595.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

280836.

KEGG

bta:280836.

Organism-specific databases

CTD

3952.

Phylogenomic databases

eggNOG

NOG45133.

HOGENOM

HOG000252923.

HOVERGEN

HBG007860.

InParanoid

P50595.

KO

K05424.

OrthoDB

EOG4QNMXC.

Family and domain databases

Gene3D

1.20.1250.10. 1 hit.

InterPro

IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR000065. Leptin.
[Graphical view]

PANTHER

PTHR11724. PTHR11724. 1 hit.

Pfam

PF02024. Leptin. 1 hit.
[Graphical view]

PIRSF

PIRSF001837. Leptin. 1 hit.

PRINTS

PR00495. LEPTIN.

ProDom

PD005698. Leptin. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF47266. 4_helix_cytokine. 1 hit.

ProtoNet

Search...

Other

NextBio

20804985.

Entry information

Entry name

LEP_BOVIN

Accession

Primary (citable) accession number: P50595
Secondary accession number(s): O97918 , Q5EAE4, Q95133, Q9TSZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program