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Protein

Tubby protein

Gene

Tub

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight. Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • phagocytosis, recognition Source: MGI
  • photoreceptor cell maintenance Source: MGI
  • positive regulation of phagocytosis Source: UniProtKB
  • protein localization to ciliary membrane Source: MGI
  • protein localization to photoreceptor outer segment Source: MGI
  • receptor localization to non-motile cilium Source: MGI
  • response to hormone Source: Ensembl
  • retina development in camera-type eye Source: MGI
  • sensory perception of sound Source: MGI
Complete GO annotation...

Keywords - Biological processi

Phagocytosis, Sensory transduction

Names & Taxonomyi

Protein namesi
Recommended name:
Tubby protein
Gene namesi
Name:Tub
Synonyms:Rd5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2651573. Tub.

Subcellular locationi

  • Cytoplasm
  • Nucleus
  • Secreted
  • Cell membrane; Peripheral membrane protein; Cytoplasmic side

  • Note: Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.

GO - Cellular componenti

  • cilium Source: GO_Central
  • cytosol Source: MGI
  • extracellular region Source: MGI
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus, Secreted

Pathology & Biotechi

Involvement in diseasei

Defects in Tub are the cause of maturity-onset obesity, insulin resistance and sensory deficits.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi330K → A: Abolishes interaction with phosphatidylinositol 4,5-bisphosphate and reduces secretion by 40%; when associated with A-332. 2 Publications1
Mutagenesisi332R → A: Abolishes interaction with phosphatidylinositol 4,5-bisphosphate and reduces secretion by 40%; when associated with A-330. 2 Publications1

Keywords - Diseasei

Obesity

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864641 – 505Tubby proteinAdd BLAST505

Proteomic databases

PaxDbiP50586.
PeptideAtlasiP50586.
PRIDEiP50586.

PTM databases

iPTMnetiP50586.
PhosphoSitePlusiP50586.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031028.
CleanExiMM_TUB.
ExpressionAtlasiP50586. baseline and differential.
GenevisibleiP50586. MM.

Interactioni

Subunit structurei

Interacts with GNAQ. Interacts with TULP1.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204371. 1 interactor.
STRINGi10090.ENSMUSP00000033341.

Structurei

Secondary structure

1505
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi250 – 255Combined sources6
Beta strandi264 – 270Combined sources7
Beta strandi275 – 278Combined sources4
Beta strandi282 – 288Combined sources7
Beta strandi294 – 315Combined sources22
Helixi317 – 320Combined sources4
Turni321 – 323Combined sources3
Beta strandi328 – 333Combined sources6
Beta strandi337 – 343Combined sources7
Beta strandi345 – 347Combined sources3
Helixi349 – 351Combined sources3
Helixi356 – 358Combined sources3
Beta strandi359 – 362Combined sources4
Beta strandi365 – 371Combined sources7
Beta strandi384 – 389Combined sources6
Helixi405 – 407Combined sources3
Helixi409 – 414Combined sources6
Beta strandi419 – 426Combined sources8
Beta strandi430 – 432Combined sources3
Turni433 – 436Combined sources4
Beta strandi437 – 442Combined sources6
Beta strandi445 – 448Combined sources4
Beta strandi453 – 457Combined sources5
Beta strandi464 – 473Combined sources10
Beta strandi476 – 481Combined sources6
Helixi487 – 499Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8ZX-ray1.90A243-505[»]
1I7EX-ray1.95A243-505[»]
ProteinModelPortaliP50586.
SMRiP50586.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP50586.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi197 – 204Asp/Glu-rich8
Compositional biasi206 – 210Poly-Ser5

Sequence similaritiesi

Belongs to the TUB family.Curated

Phylogenomic databases

eggNOGiKOG2502. Eukaryota.
ENOG410XQFT. LUCA.
GeneTreeiENSGT00610000085970.
HOGENOMiHOG000016044.
HOVERGENiHBG018010.
InParanoidiP50586.
OMAiATMQRKG.
OrthoDBiEOG091G04TF.
PhylomeDBiP50586.
TreeFamiTF314076.

Family and domain databases

Gene3Di3.20.90.10. 1 hit.
InterProiIPR000007. Tubby_C.
IPR025659. Tubby_C-like.
IPR018066. Tubby_C_CS.
IPR005398. Tubby_N.
[Graphical view]
PfamiPF01167. Tub. 1 hit.
PF16322. Tub_N. 1 hit.
[Graphical view]
PRINTSiPR01573. SUPERTUBBY.
PR01574. TUBBYPROTEIN.
SUPFAMiSSF54518. SSF54518. 1 hit.
PROSITEiPS01200. TUB_1. 1 hit.
PS01201. TUB_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P50586-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSKPHSDWI PYSVLDDEGS NLRQQKLDRQ RALLEQKQKK KRQEPLMVQA
60 70 80 90 100
NADGRPRSRR ARQSEEQAPL VESYLSSSGS TSYQVQEADS IASVQLGATR
110 120 130 140 150
PPAPASAKKS KGAAASGGQG GAPRKEKKGK HKGTSGPATL AEDKSEAQGP
160 170 180 190 200
VQILTVGQSD HDKDAGETAA GGGAQPSGQD LRATMQRKGI SSSMSFDEDE
210 220 230 240 250
DEDENSSSSS QLNSNTRPSS ATSRKSIREA ASAPSPAAPE PPVDIEVQDL
260 270 280 290 300
EEFALRPAPQ GITIKCRITR DKKGMDRGMY PTYFLHLDRE DGKKVFLLAG
310 320 330 340 350
RKRKKSKTSN YLISVDPTDL SRGGDSYIGK LRSNLMGTKF TVYDNGVNPQ
360 370 380 390 400
KASSSTLESG TLRQELAAVC YETNVLGFKG PRKMSVIVPG MNMVHERVCI
410 420 430 440 450
RPRNEHETLL ARWQNKNTES IIELQNKTPV WNDDTQSYVL NFHGRVTQAS
460 470 480 490 500
VKNFQIIHGN DPDYIVMQFG RVAEDVFTMD YNYPLCALQA FAIALSSFDS

KLACE
Length:505
Mass (Da):55,362
Last modified:October 1, 1996 - v1
Checksum:i32E160BBF5265211
GO
Isoform Short (identifier: P50586-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     153-208: Missing.

Show »
Length:449
Mass (Da):49,563
Checksum:i498167BD335C0776
GO

Sequence cautioni

The sequence AAC52512 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006675153 – 208Missing in isoform Short. CuratedAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54643 mRNA. Translation: AAB53495.1.
U52433 mRNA. Translation: AAC52510.1.
U52824 mRNA. Translation: AAC52512.1. Different initiation.
AJ296303 Genomic DNA. Translation: CAC39309.1.
CCDSiCCDS21732.1. [P50586-1]
PIRiS68518.
RefSeqiNP_068685.1. NM_021885.4. [P50586-1]
UniGeneiMm.439722.

Genome annotation databases

EnsembliENSMUST00000033341; ENSMUSP00000033341; ENSMUSG00000031028. [P50586-1]
GeneIDi22141.
KEGGimmu:22141.
UCSCiuc009jdf.1. mouse. [P50586-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54643 mRNA. Translation: AAB53495.1.
U52433 mRNA. Translation: AAC52510.1.
U52824 mRNA. Translation: AAC52512.1. Different initiation.
AJ296303 Genomic DNA. Translation: CAC39309.1.
CCDSiCCDS21732.1. [P50586-1]
PIRiS68518.
RefSeqiNP_068685.1. NM_021885.4. [P50586-1]
UniGeneiMm.439722.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C8ZX-ray1.90A243-505[»]
1I7EX-ray1.95A243-505[»]
ProteinModelPortaliP50586.
SMRiP50586.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204371. 1 interactor.
STRINGi10090.ENSMUSP00000033341.

PTM databases

iPTMnetiP50586.
PhosphoSitePlusiP50586.

Proteomic databases

PaxDbiP50586.
PeptideAtlasiP50586.
PRIDEiP50586.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033341; ENSMUSP00000033341; ENSMUSG00000031028. [P50586-1]
GeneIDi22141.
KEGGimmu:22141.
UCSCiuc009jdf.1. mouse. [P50586-1]

Organism-specific databases

CTDi7275.
MGIiMGI:2651573. Tub.

Phylogenomic databases

eggNOGiKOG2502. Eukaryota.
ENOG410XQFT. LUCA.
GeneTreeiENSGT00610000085970.
HOGENOMiHOG000016044.
HOVERGENiHBG018010.
InParanoidiP50586.
OMAiATMQRKG.
OrthoDBiEOG091G04TF.
PhylomeDBiP50586.
TreeFamiTF314076.

Miscellaneous databases

EvolutionaryTraceiP50586.
PROiP50586.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031028.
CleanExiMM_TUB.
ExpressionAtlasiP50586. baseline and differential.
GenevisibleiP50586. MM.

Family and domain databases

Gene3Di3.20.90.10. 1 hit.
InterProiIPR000007. Tubby_C.
IPR025659. Tubby_C-like.
IPR018066. Tubby_C_CS.
IPR005398. Tubby_N.
[Graphical view]
PfamiPF01167. Tub. 1 hit.
PF16322. Tub_N. 1 hit.
[Graphical view]
PRINTSiPR01573. SUPERTUBBY.
PR01574. TUBBYPROTEIN.
SUPFAMiSSF54518. SSF54518. 1 hit.
PROSITEiPS01200. TUB_1. 1 hit.
PS01201. TUB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTUB_MOUSE
AccessioniPrimary (citable) accession number: P50586
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.