Reviewed, UniProtKB/Swiss-Prot P50584 (AP4A_PIG)

Names and origin

Protein names

Recommended name:
    Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
    EC=3.6.1.17
Alternative name(s):
    Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
      Short name=Diadenosine tetraphosphatase
      Short name=Ap4A hydrolase
      Short name=Ap4Aase
    Nucleoside diphosphate-linked moiety X motif 2
      Short name=Nudix motif 2

Gene names

Name:	NUDT2
Synonyms:	APAH1

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	147 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4A to yield AMP and ATP. Plays a major role in maintaining homeostasis.
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP.
Cofactor	Divalent ions By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. Contains 1 nudix hydrolase domain.

Ontologies

Keywords
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Acetylation
Gene Ontology (GO)
Biological process	induction of apoptosis Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 147	146	Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]		PRO_0000057104
Regions
	Domain	1 – 139	139	Nudix hydrolase
	Motif	43 – 64	22	Nudix box
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P50584-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 332B6F3C185A7379	FASTA	147	16,835
	10 20 30 40 50 60 MALRACGLII FRRRLIPKVD NTAIEFLLLQ ASNGIHHWTP PKGHVEPGES DLQTALRETQ 70 80 90 100 110 120 EEAGIDAGQL TIIEGFRKEL NYVAWEKPKT VIYWLAEVKD YDVEVRLSRE HQAYRWLGLD 130 140 EACQLAQFKD MKAVLQEGHQ FLCSMAA

References

[1]

"Molecular cloning of diadenosine tetraphosphatase from pig small intestinal mucosa and identification of sequence blocks common to diadenosine polyphosphate hydrolases and phosphorylases." Hankin S., Winteroe A.K., McLennan A.G. Int. J. Biochem. Cell Biol. 29:317-323(1997) [PubMed: 9147133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Small intestine.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U38619 mRNA. Translation: AAB61380.1.
RefSeq	NP_999474.1.
UniGene	Ssc.1388
3D structure databases
SMR	P50584. Positions 1-147.
ModBase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000012018; ENSSSCP00000011710; ENSSSCG00000010981; Sus scrofa. [Genome view] ENSSSCT00000012019; ENSSSCP00000011711; ENSSSCG00000010981; Sus scrofa. [Genome view]
GeneID	397577.
KEGG	ssc:397577.
Organism-specific databases
CTD	397577.
Phylogenomic databases
HOVERGEN	P50584.
Enzyme and pathway databases
BRENDA	3.6.1.17. 249.
Family and domain databases
InterPro	IPR020084. NUDIX_hydrolase_CS. IPR000086. NUDIX_hydrolase_dom. IPR015797. NUDIX_hydrolase_dom-like. IPR003565. Tetra_PHTase. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
PRINTS	PR01405. TETRPHPHTASE.
PROSITE	PS51462. NUDIX. 1 hit. PS00893. NUDIX_BOX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AP4A_PIG

Accession

Primary (citable) accession number: P50584

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)