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P50580

- PA2G4_MOUSE

UniProt

P50580 - PA2G4_MOUSE

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Protein
Proliferation-associated protein 2G4
Gene
Pa2g4, Ebp1, Plfap
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly By similarity. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Together with PTBP1 is required for the translation initiation on the foot-and-mouth disease virus (FMDV) IRES.2 Publications

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA binding Source: UniProtKB-KW
  3. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: MGI
  2. rRNA processing Source: UniProtKB-KW
  3. regulation of translation Source: UniProtKB-KW
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor, Ribonucleoprotein

Keywords - Biological processi

rRNA processing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

RNA-binding

Protein family/group databases

MEROPSiM24.973.

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferation-associated protein 2G4
Alternative name(s):
IRES-specific cellular trans-acting factor 45 kDa
Short name:
ITAF45
Mpp1
Proliferation-associated protein 1
Protein p38-2G4
Gene namesi
Name:Pa2g4
Synonyms:Ebp1, Plfap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:894684. Pa2g4.

Subcellular locationi

Cytoplasm By similarity. Nucleusnucleolus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleolus Source: UniProtKB-SubCell
  3. nucleus Source: MGI
  4. ribonucleoprotein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi65 – 728KKEKEMKK → SSSSSSSS: No effect on RNA-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 394393Proliferation-associated protein 2G4
PRO_0000148990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei2 – 21Phosphoserine1 Publication
Modified residuei361 – 3611Phosphoserine By similarity
Modified residuei386 – 3861Phosphothreonine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP50580.
PaxDbiP50580.
PRIDEiP50580.

PTM databases

PhosphoSiteiP50580.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Expressed in proliferating cells. Observed between G1 and mid S phase, decrease toward the end of S phase, and disappear at the S/G2 transition.

Inductioni

By mitogens.

Gene expression databases

ArrayExpressiP50580.
BgeeiP50580.
CleanExiMM_PA2G4.
GenevestigatoriP50580.

Interactioni

Subunit structurei

Interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with nucleolin/NCL. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation By similarity.1 Publication

Protein-protein interaction databases

BioGridi202246. 3 interactions.
IntActiP50580. 7 interactions.
MINTiMINT-1857756.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 3622
Helixi45 – 6016
Beta strandi72 – 8211
Beta strandi85 – 873
Beta strandi93 – 953
Beta strandi105 – 11410
Beta strandi117 – 12610
Helixi138 – 15619
Helixi163 – 17614
Beta strandi186 – 1894
Beta strandi194 – 1963
Beta strandi198 – 2047
Helixi207 – 2126
Beta strandi223 – 23311
Beta strandi246 – 2494
Helixi260 – 27213
Turni273 – 2753
Helixi280 – 2823
Helixi286 – 29813
Beta strandi301 – 3055
Beta strandi316 – 32611
Beta strandi329 – 3324
Helixi340 – 3423
Helixi352 – 3576

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V6CX-ray2.50A9-360[»]
ProteinModelPortaliP50580.
SMRiP50580. Positions 8-360.

Miscellaneous databases

EvolutionaryTraceiP50580.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 4847Necessary for nucleolar localization By similarity
Add
BLAST
Regioni46 – 549RNA-binding By similarity
Regioni301 – 39494Necessary for nucleolar localization By similarity
Add
BLAST
Regioni361 – 37515Interaction with RNA
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase M24 family.

Phylogenomic databases

eggNOGiCOG0024.
HOGENOMiHOG000168207.
HOVERGENiHBG053117.
InParanoidiP50580.
OMAiICHFSPI.
OrthoDBiEOG7SFHXD.
PhylomeDBiP50580.
TreeFamiTF300010.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
InterProiIPR004545. Pap_1.
IPR000994. Pept_M24_structural-domain.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
SUPFAMiSSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00495. crvDNA_42K. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50580-1 [UniParc]FASTAAdd to Basket

« Hide

MSGEDEQQEQ TIAEDLVVTK YKMGGDIANR VLRSLVEASS SGVSVLSLCE    50
KGDAMIMEET GKIFKKEKEM KKGIAFPTSI SVNNCVCHFS PLKSDQDYIL 100
KEGDLVKIDL GVHVDGFIAN VAHTFVIGVA QGTQVTGRKA DVIKAAHLCA 150
EAALRLVKPG NQNTQVTEAW NKVAHSFNCT PIEGMLSHQL KQHVIDGEKT 200
IIQNPTDQQK KDHEKAEFEV HEVYAVDVLV SSGEGKAKDA GQRTTIYKRD 250
PSKQYGLKMK TSRAFFSEVE RRFDAMPFTL RAFEDEKKAR MGVVECAKHE 300
LLQPFNVLYE KEGEFVAQFK FTVLLMPNGP MRITSGPFEP DLYKSEMEVQ 350
DAELKALLQS SASRKTQKKK KKKASKTVEN ATSGETLEEN GAGD 394
Length:394
Mass (Da):43,699
Last modified:January 23, 2007 - v3
Checksum:iABCD169F064261EB
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti279 – 2791T → A1 Publication
Sequence conflicti311 – 3111K → R1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84789 mRNA. Translation: CAA59260.1. Sequence problems.
U43918 mRNA. Translation: AAB60513.1.
BC046532 mRNA. Translation: AAH46532.1.
CCDSiCCDS24282.1.
PIRiI48702. S54181.
RefSeqiNP_035249.1. NM_011119.3.
UniGeneiMm.4742.

Genome annotation databases

EnsembliENSMUST00000026425; ENSMUSP00000026425; ENSMUSG00000025364.
GeneIDi18813.
KEGGimmu:18813.
UCSCiuc007hnl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84789 mRNA. Translation: CAA59260.1 . Sequence problems.
U43918 mRNA. Translation: AAB60513.1 .
BC046532 mRNA. Translation: AAH46532.1 .
CCDSi CCDS24282.1.
PIRi I48702. S54181.
RefSeqi NP_035249.1. NM_011119.3.
UniGenei Mm.4742.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2V6C X-ray 2.50 A 9-360 [» ]
ProteinModelPortali P50580.
SMRi P50580. Positions 8-360.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202246. 3 interactions.
IntActi P50580. 7 interactions.
MINTi MINT-1857756.

Protein family/group databases

MEROPSi M24.973.

PTM databases

PhosphoSitei P50580.

Proteomic databases

MaxQBi P50580.
PaxDbi P50580.
PRIDEi P50580.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026425 ; ENSMUSP00000026425 ; ENSMUSG00000025364 .
GeneIDi 18813.
KEGGi mmu:18813.
UCSCi uc007hnl.1. mouse.

Organism-specific databases

CTDi 5036.
MGIi MGI:894684. Pa2g4.

Phylogenomic databases

eggNOGi COG0024.
HOGENOMi HOG000168207.
HOVERGENi HBG053117.
InParanoidi P50580.
OMAi ICHFSPI.
OrthoDBi EOG7SFHXD.
PhylomeDBi P50580.
TreeFami TF300010.

Miscellaneous databases

ChiTaRSi PA2G4. mouse.
EvolutionaryTracei P50580.
NextBioi 295158.
PROi P50580.
SOURCEi Search...

Gene expression databases

ArrayExpressi P50580.
Bgeei P50580.
CleanExi MM_PA2G4.
Genevestigatori P50580.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
InterProi IPR004545. Pap_1.
IPR000994. Pept_M24_structural-domain.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00557. Peptidase_M24. 1 hit.
[Graphical view ]
SUPFAMi SSF55920. SSF55920. 2 hits.
TIGRFAMsi TIGR00495. crvDNA_42K. 1 hit.
PROSITEi PS01202. MAP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a murine cDNA encoding a novel protein, p38-2G4, which varies with the cell cycle."
    Radomski N., Jost E.
    Exp. Cell Res. 220:434-445(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NFS.
  2. "cDNA cloning, sequence analysis and expression of a mouse 44-kDa nuclear protein copurified with DNA repair factors for acid-depurinated DNA."
    Nakagawa Y., Watanabe S., Akiyama K., Sarker A.H., Tsutsui K., Inoue H., Seki S.
    Acta Med. Okayama 51:195-206(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NFS.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  4. "A cell cycle-dependent protein serves as a template-specific translation initiation factor."
    Pilipenko E.V., Pestova T.V., Kolupaeva V.G., Khitrina E.V., Poperechnaya A.N., Agol V.I., Hellen C.U.
    Genes Dev. 14:2028-2045(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA-BINDING, FUNCTION IN VIRAL TRANSLATION.
  5. "EBP1 is a nucleolar growth-regulating protein that is part of pre-ribosomal ribonucleoprotein complexes."
    Squatrito M., Mancino M., Donzelli M., Areces L.B., Draetta G.F.
    Oncogene 23:4454-4465(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 8-360, ABSENCE OF METAL COFACTOR AND OF AMINOPEPTIDASE ACTIVITY, FUNCTION, MUTAGENESIS OF 65-LYS--LYS-72, INTERACTION WITH RNA.

Entry informationi

Entry nameiPA2G4_MOUSE
AccessioniPrimary (citable) accession number: P50580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Although it belongs to the peptidase M24 family, it does not contain metal cofactors and lacks aminopeptidase activity.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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