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P50580

- PA2G4_MOUSE

UniProt

P50580 - PA2G4_MOUSE

Protein

Proliferation-associated protein 2G4

Gene

Pa2g4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly By similarity. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Together with PTBP1 is required for the translation initiation on the foot-and-mouth disease virus (FMDV) IRES.By similarity2 Publications

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. RNA binding Source: UniProtKB-KW
    3. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. negative regulation of transcription, DNA-templated Source: MGI
    2. regulation of translation Source: UniProtKB-KW
    3. rRNA processing Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor, Ribonucleoprotein

    Keywords - Biological processi

    rRNA processing, Transcription, Transcription regulation, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Protein family/group databases

    MEROPSiM24.973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proliferation-associated protein 2G4
    Alternative name(s):
    IRES-specific cellular trans-acting factor 45 kDa
    Short name:
    ITAF45
    Mpp1
    Proliferation-associated protein 1
    Protein p38-2G4
    Gene namesi
    Name:Pa2g4
    Synonyms:Ebp1, Plfap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:894684. Pa2g4.

    Subcellular locationi

    Cytoplasm By similarity. Nucleusnucleolus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleolus Source: UniProtKB-SubCell
    3. nucleus Source: MGI
    4. ribonucleoprotein complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi65 – 728KKEKEMKK → SSSSSSSS: No effect on RNA-binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 394393Proliferation-associated protein 2G4PRO_0000148990Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei2 – 21Phosphoserine1 Publication
    Modified residuei361 – 3611PhosphoserineBy similarity
    Modified residuei386 – 3861PhosphothreonineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP50580.
    PaxDbiP50580.
    PRIDEiP50580.

    PTM databases

    PhosphoSiteiP50580.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Developmental stagei

    Expressed in proliferating cells. Observed between G1 and mid S phase, decrease toward the end of S phase, and disappear at the S/G2 transition.

    Inductioni

    By mitogens.

    Gene expression databases

    ArrayExpressiP50580.
    BgeeiP50580.
    CleanExiMM_PA2G4.
    GenevestigatoriP50580.

    Interactioni

    Subunit structurei

    Interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with nucleolin/NCL. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202246. 3 interactions.
    IntActiP50580. 7 interactions.
    MINTiMINT-1857756.

    Structurei

    Secondary structure

    1
    394
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi15 – 3622
    Helixi45 – 6016
    Beta strandi72 – 8211
    Beta strandi85 – 873
    Beta strandi93 – 953
    Beta strandi105 – 11410
    Beta strandi117 – 12610
    Helixi138 – 15619
    Helixi163 – 17614
    Beta strandi186 – 1894
    Beta strandi194 – 1963
    Beta strandi198 – 2047
    Helixi207 – 2126
    Beta strandi223 – 23311
    Beta strandi246 – 2494
    Helixi260 – 27213
    Turni273 – 2753
    Helixi280 – 2823
    Helixi286 – 29813
    Beta strandi301 – 3055
    Beta strandi316 – 32611
    Beta strandi329 – 3324
    Helixi340 – 3423
    Helixi352 – 3576

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2V6CX-ray2.50A9-360[»]
    ProteinModelPortaliP50580.
    SMRiP50580. Positions 8-360.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP50580.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 4847Necessary for nucleolar localizationBy similarityAdd
    BLAST
    Regioni46 – 549RNA-bindingBy similarity
    Regioni301 – 39494Necessary for nucleolar localizationBy similarityAdd
    BLAST
    Regioni361 – 37515Interaction with RNAAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M24 family.Curated

    Phylogenomic databases

    eggNOGiCOG0024.
    HOGENOMiHOG000168207.
    HOVERGENiHBG053117.
    InParanoidiP50580.
    OMAiICHFSPI.
    OrthoDBiEOG7SFHXD.
    PhylomeDBiP50580.
    TreeFamiTF300010.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    InterProiIPR004545. Pap_1.
    IPR000994. Pept_M24_structural-domain.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00557. Peptidase_M24. 1 hit.
    [Graphical view]
    SUPFAMiSSF55920. SSF55920. 2 hits.
    TIGRFAMsiTIGR00495. crvDNA_42K. 1 hit.
    PROSITEiPS01202. MAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50580-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGEDEQQEQ TIAEDLVVTK YKMGGDIANR VLRSLVEASS SGVSVLSLCE    50
    KGDAMIMEET GKIFKKEKEM KKGIAFPTSI SVNNCVCHFS PLKSDQDYIL 100
    KEGDLVKIDL GVHVDGFIAN VAHTFVIGVA QGTQVTGRKA DVIKAAHLCA 150
    EAALRLVKPG NQNTQVTEAW NKVAHSFNCT PIEGMLSHQL KQHVIDGEKT 200
    IIQNPTDQQK KDHEKAEFEV HEVYAVDVLV SSGEGKAKDA GQRTTIYKRD 250
    PSKQYGLKMK TSRAFFSEVE RRFDAMPFTL RAFEDEKKAR MGVVECAKHE 300
    LLQPFNVLYE KEGEFVAQFK FTVLLMPNGP MRITSGPFEP DLYKSEMEVQ 350
    DAELKALLQS SASRKTQKKK KKKASKTVEN ATSGETLEEN GAGD 394
    Length:394
    Mass (Da):43,699
    Last modified:January 23, 2007 - v3
    Checksum:iABCD169F064261EB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti279 – 2791T → A(PubMed:10950867)Curated
    Sequence conflicti311 – 3111K → R(PubMed:10950867)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84789 mRNA. Translation: CAA59260.1. Sequence problems.
    U43918 mRNA. Translation: AAB60513.1.
    BC046532 mRNA. Translation: AAH46532.1.
    CCDSiCCDS24282.1.
    PIRiI48702. S54181.
    RefSeqiNP_035249.1. NM_011119.3.
    UniGeneiMm.4742.

    Genome annotation databases

    EnsembliENSMUST00000026425; ENSMUSP00000026425; ENSMUSG00000025364.
    GeneIDi18813.
    KEGGimmu:18813.
    UCSCiuc007hnl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84789 mRNA. Translation: CAA59260.1 . Sequence problems.
    U43918 mRNA. Translation: AAB60513.1 .
    BC046532 mRNA. Translation: AAH46532.1 .
    CCDSi CCDS24282.1.
    PIRi I48702. S54181.
    RefSeqi NP_035249.1. NM_011119.3.
    UniGenei Mm.4742.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2V6C X-ray 2.50 A 9-360 [» ]
    ProteinModelPortali P50580.
    SMRi P50580. Positions 8-360.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202246. 3 interactions.
    IntActi P50580. 7 interactions.
    MINTi MINT-1857756.

    Protein family/group databases

    MEROPSi M24.973.

    PTM databases

    PhosphoSitei P50580.

    Proteomic databases

    MaxQBi P50580.
    PaxDbi P50580.
    PRIDEi P50580.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026425 ; ENSMUSP00000026425 ; ENSMUSG00000025364 .
    GeneIDi 18813.
    KEGGi mmu:18813.
    UCSCi uc007hnl.1. mouse.

    Organism-specific databases

    CTDi 5036.
    MGIi MGI:894684. Pa2g4.

    Phylogenomic databases

    eggNOGi COG0024.
    HOGENOMi HOG000168207.
    HOVERGENi HBG053117.
    InParanoidi P50580.
    OMAi ICHFSPI.
    OrthoDBi EOG7SFHXD.
    PhylomeDBi P50580.
    TreeFami TF300010.

    Miscellaneous databases

    ChiTaRSi PA2G4. mouse.
    EvolutionaryTracei P50580.
    NextBioi 295158.
    PROi P50580.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P50580.
    Bgeei P50580.
    CleanExi MM_PA2G4.
    Genevestigatori P50580.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.90.230.10. 2 hits.
    InterProi IPR004545. Pap_1.
    IPR000994. Pept_M24_structural-domain.
    IPR018349. Pept_M24A_MAP2_BS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00557. Peptidase_M24. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55920. SSF55920. 2 hits.
    TIGRFAMsi TIGR00495. crvDNA_42K. 1 hit.
    PROSITEi PS01202. MAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a murine cDNA encoding a novel protein, p38-2G4, which varies with the cell cycle."
      Radomski N., Jost E.
      Exp. Cell Res. 220:434-445(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: NFS.
    2. "cDNA cloning, sequence analysis and expression of a mouse 44-kDa nuclear protein copurified with DNA repair factors for acid-depurinated DNA."
      Nakagawa Y., Watanabe S., Akiyama K., Sarker A.H., Tsutsui K., Inoue H., Seki S.
      Acta Med. Okayama 51:195-206(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: NFS.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    4. "A cell cycle-dependent protein serves as a template-specific translation initiation factor."
      Pilipenko E.V., Pestova T.V., Kolupaeva V.G., Khitrina E.V., Poperechnaya A.N., Agol V.I., Hellen C.U.
      Genes Dev. 14:2028-2045(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], RNA-BINDING, FUNCTION IN VIRAL TRANSLATION.
    5. "EBP1 is a nucleolar growth-regulating protein that is part of pre-ribosomal ribonucleoprotein complexes."
      Squatrito M., Mancino M., Donzelli M., Areces L.B., Draetta G.F.
      Oncogene 23:4454-4465(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    7. Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 8-360, ABSENCE OF METAL COFACTOR AND OF AMINOPEPTIDASE ACTIVITY, FUNCTION, MUTAGENESIS OF 65-LYS--LYS-72, INTERACTION WITH RNA.

    Entry informationi

    Entry nameiPA2G4_MOUSE
    AccessioniPrimary (citable) accession number: P50580
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Although it belongs to the peptidase M24 family, it does not contain metal cofactors and lacks aminopeptidase activity.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3