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Protein

Gamma-aminobutyric acid receptor subunit beta-1

Gene

Gabrb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251AgonistBy similarity

GO - Molecular functioni

  1. chloride channel activity Source: UniProtKB-KW
  2. extracellular ligand-gated ion channel activity Source: InterPro
  3. GABA-A receptor activity Source: UniProtKB
  4. ligand-gated ion channel activity Source: UniProtKB

GO - Biological processi

  1. cellular response to histamine Source: UniProtKB
  2. ion transmembrane transport Source: GOC
  3. ion transport Source: UniProtKB
  4. signal transduction Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiREACT_293019. Ligand-gated ion channel transport.
REACT_297274. Orphan transporters.
REACT_343799. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit beta-1
Alternative name(s):
GABA(A) receptor subunit beta-1
Gene namesi
Name:Gabrb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:95619. Gabrb1.

Subcellular locationi

Cell junctionsynapsepostsynaptic cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 245220ExtracellularCuratedAdd
BLAST
Transmembranei246 – 26722HelicalCuratedAdd
BLAST
Transmembranei271 – 29323HelicalCuratedAdd
BLAST
Transmembranei305 – 32723HelicalCuratedAdd
BLAST
Topological domaini328 – 451124CytoplasmicCuratedAdd
BLAST
Transmembranei452 – 47322HelicalCuratedAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. chloride channel complex Source: UniProtKB-KW
  3. GABA-A receptor complex Source: UniProtKB
  4. integral component of plasma membrane Source: UniProtKB
  5. postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 474449Gamma-aminobutyric acid receptor subunit beta-1PRO_0000000457Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi161 ↔ 175By similarity
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP50571.
PaxDbiP50571.
PRIDEiP50571.

PTM databases

PhosphoSiteiP50571.

Expressioni

Gene expression databases

BgeeiP50571.
ExpressionAtlasiP50571. baseline and differential.
GenevestigatoriP50571.

Interactioni

Subunit structurei

Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains. Binds UBQLN1. Interacts with KCTD8, KCTD12, KCTD12B and KCTD16; this interaction determines the pharmacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents.2 Publications

Protein-protein interaction databases

BioGridi199802. 2 interactions.
STRINGi10090.ENSMUSP00000031122.

Structurei

3D structure databases

ProteinModelPortaliP50571.
SMRiP50571. Positions 35-341, 448-473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni120 – 1223Agonist bindingBy similarity
Regioni180 – 1823Agonist bindingBy similarity
Regioni290 – 31122Allosteric effector bindingBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG77438.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP50571.
KOiK05181.
OMAiERSETCS.
OrthoDBiEOG712TVZ.
PhylomeDBiP50571.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50571-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTVQNRESL GLLSFPVMVA MVCCAHSSNE PSNMSYVKET VDRLLKGYDI
60 70 80 90 100
RLRPDFGGPP VDVGMRIDVA SIDMVSEVNM DYTLTMYFQQ SWKDKRLSYS
110 120 130 140 150
GIPLNLTLDN RVADQLWVPD TYFLNDKKSF VHGVTVKNRM IRLHPDGTVL
160 170 180 190 200
YGLRITTTAA CMMDLRRYPL DEQNCTLEIE SYGYTTDDIE FYWNGGEGAV
210 220 230 240 250
TGVNKIELPQ FSIVDYKMVS KKVEFTTGAY PRLSLSFRLK RNIGYFILQT
260 270 280 290 300
YMPSTLITIL SWVSFWINYD ASAARVALGI TTVLTMTTIS THLRETLPKI
310 320 330 340 350
PYVKAIDIYL MGCFVFVFLA LLEYAFVNYI FFGKGPQKKG ASKQDQSANE
360 370 380 390 400
KNRLEMNKVQ VDAHGNILLS TLEIRNETSG SEVLTGVSDP KATMYSYDSA
410 420 430 440 450
SIQYRKPLSS REGFGRGLDR HGVPGKGRIR RRASQLKVKI PDLTDVNSID
460 470
KWSRMFFPIT FSLFNVVYWL YYVH
Length:474
Mass (Da):54,100
Last modified:September 30, 1996 - v1
Checksum:i986C47E4BD663763
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14418 mRNA. Translation: AAA79973.1.
CCDSiCCDS19329.1.
PIRiS53530.
RefSeqiNP_032095.1. NM_008069.4.
UniGeneiMm.38567.

Genome annotation databases

EnsembliENSMUST00000031122; ENSMUSP00000031122; ENSMUSG00000029212.
GeneIDi14400.
KEGGimmu:14400.
UCSCiuc008xrb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14418 mRNA. Translation: AAA79973.1.
CCDSiCCDS19329.1.
PIRiS53530.
RefSeqiNP_032095.1. NM_008069.4.
UniGeneiMm.38567.

3D structure databases

ProteinModelPortaliP50571.
SMRiP50571. Positions 35-341, 448-473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199802. 2 interactions.
STRINGi10090.ENSMUSP00000031122.

Chemistry

ChEMBLiCHEMBL2094133.
GuidetoPHARMACOLOGYi410.

PTM databases

PhosphoSiteiP50571.

Proteomic databases

MaxQBiP50571.
PaxDbiP50571.
PRIDEiP50571.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031122; ENSMUSP00000031122; ENSMUSG00000029212.
GeneIDi14400.
KEGGimmu:14400.
UCSCiuc008xrb.1. mouse.

Organism-specific databases

CTDi2560.
MGIiMGI:95619. Gabrb1.

Phylogenomic databases

eggNOGiNOG77438.
HOGENOMiHOG000231335.
HOVERGENiHBG051707.
InParanoidiP50571.
KOiK05181.
OMAiERSETCS.
OrthoDBiEOG712TVZ.
PhylomeDBiP50571.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiREACT_293019. Ligand-gated ion channel transport.
REACT_297274. Orphan transporters.
REACT_343799. GABA A receptor activation.

Miscellaneous databases

ChiTaRSiGabrb1. mouse.
NextBioi285945.
PROiP50571.
SOURCEiSearch...

Gene expression databases

BgeeiP50571.
ExpressionAtlasiP50571. baseline and differential.
GenevestigatoriP50571.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR002289. GABAAb_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01160. GABAARBETA.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "GABAA receptor beta 1, beta 2, and beta 3 subunits: comparisons in DBA/2J and C57BL/6J mice."
    Kamatchi G.L., Kofuji P., Wang J.B., Fernando J.C., Liu Z., Mathura J.R., Burt D.R.
    Biochim. Biophys. Acta 1261:134-142(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J and DBA/2J.
    Tissue: Brain.
  2. "GABA(A) receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1."
    Bedford F.K., Kittler J.T., Muller E., Thomas P., Uren J.M., Merlo D., Wisden W., Triller A., Smart T.G., Moss S.J.
    Nat. Neurosci. 4:908-916(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBQLN1.
  3. Cited for: INTERACTION WITH KCTD8; KCTD12; KCTD12B AND KCTD16, FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGBRB1_MOUSE
AccessioniPrimary (citable) accession number: P50571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: September 30, 1996
Last modified: March 31, 2015
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.