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P50554

- GABT_RAT

UniProt

P50554 - GABT_RAT

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Protein

4-aminobutyrate aminotransferase, mitochondrial

Gene
Abat, Gabat
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.

Catalytic activityi

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.

Cofactori

Pyridoxal phosphate.

GO - Molecular functioni

  1. (S)-3-amino-2-methylpropionate transaminase activity Source: UniProtKB-EC
  2. 4-aminobutyrate transaminase activity Source: RGD
  3. protein homodimerization activity Source: UniProtKB
  4. pyridoxal phosphate binding Source: UniProtKB
  5. succinate-semialdehyde dehydrogenase binding Source: UniProtKB

GO - Biological processi

  1. behavioral response to cocaine Source: UniProtKB
  2. copulation Source: RGD
  3. gamma-aminobutyric acid catabolic process Source: UniProtKB
  4. locomotory behavior Source: RGD
  5. negative regulation of blood pressure Source: RGD
  6. neurotransmitter catabolic process Source: UniProtKB
  7. response to cocaine Source: RGD
  8. response to drug Source: RGD
  9. response to ethanol Source: RGD
  10. response to hypoxia Source: RGD
  11. response to iron ion Source: RGD
  12. response to nicotine Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Neurotransmitter degradation

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_196229. Degradation of GABA.
SABIO-RKP50554.

Names & Taxonomyi

Protein namesi
Recommended name:
4-aminobutyrate aminotransferase, mitochondrial (EC:2.6.1.19)
Alternative name(s):
(S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22)
GABA aminotransferase
Short name:
GABA-AT
Gamma-amino-N-butyrate transaminase
Short name:
GABA transaminase
Short name:
GABA-T
L-AIBAT
Cleaved into the following 2 chains:
Gene namesi
Name:Abat
Synonyms:Gabat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi620948. Abat.

Subcellular locationi

GO - Cellular componenti

  1. 4-aminobutyrate transaminase complex Source: UniProtKB
  2. mitochondrial matrix Source: UniProtKB-SubCell
  3. mitochondrion Source: UniProtKB
  4. neuron projection Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionAdd
BLAST
Chaini28 – 5004734-aminobutyrate aminotransferase, brain isoformPRO_0000001252Add
BLAST
Chaini35 – 5004664-aminobutyrate aminotransferase, liver isoformPRO_0000001253Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi163 ↔ 166 By similarity
Modified residuei231 – 2311N6-succinyllysine By similarity
Modified residuei252 – 2521N6-acetyllysine; alternate By similarity
Modified residuei252 – 2521N6-succinyllysine; alternate By similarity
Modified residuei279 – 2791N6-acetyllysine By similarity
Modified residuei318 – 3181N6-acetyllysine By similarity
Disulfide bondi321 – 321Interchain By similarity
Modified residuei357 – 3571N6-(pyridoxal phosphate)lysine
Modified residuei413 – 4131N6-acetyllysine; alternate By similarity
Modified residuei413 – 4131N6-succinyllysine; alternate By similarity
Modified residuei452 – 4521N6-acetyllysine By similarity
Modified residuei470 – 4701N6-acetyllysine By similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiP50554.
PRIDEiP50554.

2D gel databases

World-2DPAGE0004:P50554.

PTM databases

PhosphoSiteiP50554.

Expressioni

Gene expression databases

GenevestigatoriP50554.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003633.

Structurei

3D structure databases

ProteinModelPortaliP50554.
SMRiP50554. Positions 39-499.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0160.
GeneTreeiENSGT00550000074885.
HOGENOMiHOG000020208.
HOVERGENiHBG000634.
InParanoidiP50554.
KOiK13524.
OMAiKLIQQPQ.
OrthoDBiEOG7ZPNJW.
PhylomeDBiP50554.
TreeFamiTF105021.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR004631. 4NH2But_aminotransferase_euk.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PTHR11986:SF6. PTHR11986:SF6. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P50554-1 [UniParc]FASTAAdd to Basket

« Hide

MAFLLTTRRL VCSSQKNLHL FTPGSRYISQ AAAKVDFEFD YDGPLMKTEV    50
PGPRSQELMK QLNTIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI 100
SSVPIGYNHP ALAKLVQQPQ NASTFINRPA LGILPPENFV DKLRESLMSV 150
APKGMCQLIT MACGSCSNEN AFKTIFMWYR SKERGQRGFS KEELETCMVN 200
QSPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS FDWPIAPFPR 250
LKYPLEEFVT DNQQEEARCL EEVEDLIVKY RKKKRTVAGI IVEPIQSEGG 300
DNHASDDFFR KLRDIARKHG CAFLVDEVQT GGGCTGKFWA HEHWGLDDPA 350
DVMSFSKKMM TGGFFHKEEF RPSAPYRIFN TWLGDPSKNL LLAEVINIIK 400
REDLLNNVAH AGKTLLTGLL DLQAQYPQFV SRVRGRGTFC SFDTPDEAIR 450
NKLILIARNK GVVLGGCGDK SIRFRPTLVF RDHHAHLFLN IFSGILADFK 500
Length:500
Mass (Da):56,456
Last modified:January 9, 2007 - v3
Checksum:i1EA7180B72797B72
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31F → L in AAA70415. 1 Publication
Sequence conflicti25 – 251S → T in AAA70415. 1 Publication
Sequence conflicti36 – 361D → T in AAA70415. 1 Publication
Sequence conflicti135 – 17036PPENF…CSNEN → LQRTLWTSSGSPCSRWLPKA CVSSSRWPAGPAPMRM in AAA70415. 1 PublicationAdd
BLAST
Sequence conflicti277 – 2771I → N in AAA70415. 1 Publication
Sequence conflicti425 – 4251Q → R in AAA70415. 1 Publication
Sequence conflicti447 – 4471E → K1 Publication
Sequence conflicti447 – 4471E → K in BAA25570. 1 Publication
Sequence conflicti486 – 4861H → Q in AAA70415. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U29701 mRNA. Translation: AAA70415.1.
D87839 mRNA. Translation: BAA25570.1.
BC081787 mRNA. Translation: AAH81787.1.
PIRiI56502.
RefSeqiNP_112265.1. NM_031003.2.
XP_006245822.1. XM_006245760.1.
UniGeneiRn.10090.

Genome annotation databases

EnsembliENSRNOT00000003633; ENSRNOP00000003633; ENSRNOG00000002636.
GeneIDi81632.
KEGGirno:81632.
UCSCiRGD:620948. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U29701 mRNA. Translation: AAA70415.1 .
D87839 mRNA. Translation: BAA25570.1 .
BC081787 mRNA. Translation: AAH81787.1 .
PIRi I56502.
RefSeqi NP_112265.1. NM_031003.2.
XP_006245822.1. XM_006245760.1.
UniGenei Rn.10090.

3D structure databases

ProteinModelPortali P50554.
SMRi P50554. Positions 39-499.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000003633.

Chemistry

BindingDBi P50554.
ChEMBLi CHEMBL3148.

PTM databases

PhosphoSitei P50554.

2D gel databases

World-2DPAGE 0004:P50554.

Proteomic databases

PaxDbi P50554.
PRIDEi P50554.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000003633 ; ENSRNOP00000003633 ; ENSRNOG00000002636 .
GeneIDi 81632.
KEGGi rno:81632.
UCSCi RGD:620948. rat.

Organism-specific databases

CTDi 18.
RGDi 620948. Abat.

Phylogenomic databases

eggNOGi COG0160.
GeneTreei ENSGT00550000074885.
HOGENOMi HOG000020208.
HOVERGENi HBG000634.
InParanoidi P50554.
KOi K13524.
OMAi KLIQQPQ.
OrthoDBi EOG7ZPNJW.
PhylomeDBi P50554.
TreeFami TF105021.

Enzyme and pathway databases

Reactomei REACT_196229. Degradation of GABA.
SABIO-RK P50554.

Miscellaneous databases

NextBioi 615107.
PROi P50554.

Gene expression databases

Genevestigatori P50554.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProi IPR004631. 4NH2But_aminotransferase_euk.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
PANTHERi PTHR11986. PTHR11986. 1 hit.
PTHR11986:SF6. PTHR11986:SF6. 1 hit.
Pfami PF00202. Aminotran_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR00699. GABAtrns_euk. 1 hit.
PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A rat brain cDNA encodes enzymatically active GABA transaminase and provides a molecular probe for GABA-catabolizing cells."
    Medina-Kauwe L.K., Tillakaratne N.J., Wu J.Y., Tobin A.J.
    J. Neurochem. 62:1267-1275(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "The mature size of rat 4-aminobutyrate aminotransferase is different in liver and brain."
    Kontani Y., Sakata S.F., Matsuda K., Ohyama T., Sano K., Tamaki N.
    Eur. J. Biochem. 264:218-222(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-54, PROTEOLYTIC PROCESSING.
    Strain: Sprague-Dawley.
    Tissue: Brain and Liver.
  3. "Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver."
    Tamaki N., Sakata S.F., Matsuda K.
    Methods Enzymol. 324:376-389(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  5. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 253-268 AND 389-400, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.

Entry informationi

Entry nameiGABT_RAT
AccessioniPrimary (citable) accession number: P50554
Secondary accession number(s): O70539, Q66HM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 9, 2007
Last modified: September 3, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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