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P50554

- GABT_RAT

UniProt

P50554 - GABT_RAT

Protein

4-aminobutyrate aminotransferase, mitochondrial

Gene

Abat

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.

    Catalytic activityi

    4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
    (S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.

    GO - Molecular functioni

    1. (S)-3-amino-2-methylpropionate transaminase activity Source: UniProtKB-EC
    2. 4-aminobutyrate transaminase activity Source: RGD
    3. protein homodimerization activity Source: UniProtKB
    4. pyridoxal phosphate binding Source: UniProtKB
    5. succinate-semialdehyde dehydrogenase binding Source: UniProtKB

    GO - Biological processi

    1. behavioral response to cocaine Source: UniProtKB
    2. copulation Source: RGD
    3. gamma-aminobutyric acid catabolic process Source: UniProtKB
    4. locomotory behavior Source: RGD
    5. negative regulation of blood pressure Source: RGD
    6. neurotransmitter catabolic process Source: UniProtKB
    7. response to cocaine Source: RGD
    8. response to drug Source: RGD
    9. response to ethanol Source: RGD
    10. response to hypoxia Source: RGD
    11. response to iron ion Source: RGD
    12. response to nicotine Source: RGD

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Neurotransmitter degradation

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_196229. Degradation of GABA.
    SABIO-RKP50554.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-aminobutyrate aminotransferase, mitochondrial (EC:2.6.1.19)
    Alternative name(s):
    (S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22)
    GABA aminotransferase
    Short name:
    GABA-AT
    Gamma-amino-N-butyrate transaminase
    Short name:
    GABA transaminase
    Short name:
    GABA-T
    L-AIBAT
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Abat
    Synonyms:Gabat
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi620948. Abat.

    Subcellular locationi

    GO - Cellular componenti

    1. 4-aminobutyrate transaminase complex Source: UniProtKB
    2. mitochondrial matrix Source: UniProtKB-SubCell
    3. mitochondrion Source: UniProtKB
    4. neuron projection Source: RGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionAdd
    BLAST
    Chaini28 – 5004734-aminobutyrate aminotransferase, brain isoformPRO_0000001252Add
    BLAST
    Chaini35 – 5004664-aminobutyrate aminotransferase, liver isoformPRO_0000001253Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi163 ↔ 166By similarity
    Modified residuei231 – 2311N6-succinyllysineBy similarity
    Modified residuei252 – 2521N6-acetyllysine; alternateBy similarity
    Modified residuei252 – 2521N6-succinyllysine; alternateBy similarity
    Modified residuei279 – 2791N6-acetyllysineBy similarity
    Modified residuei318 – 3181N6-acetyllysineBy similarity
    Disulfide bondi321 – 321InterchainBy similarity
    Modified residuei357 – 3571N6-(pyridoxal phosphate)lysine
    Modified residuei413 – 4131N6-acetyllysine; alternateBy similarity
    Modified residuei413 – 4131N6-succinyllysine; alternateBy similarity
    Modified residuei452 – 4521N6-acetyllysineBy similarity
    Modified residuei470 – 4701N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond

    Proteomic databases

    PaxDbiP50554.
    PRIDEiP50554.

    2D gel databases

    World-2DPAGE0004:P50554.

    PTM databases

    PhosphoSiteiP50554.

    Expressioni

    Gene expression databases

    GenevestigatoriP50554.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000003633.

    Structurei

    3D structure databases

    ProteinModelPortaliP50554.
    SMRiP50554. Positions 39-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0160.
    GeneTreeiENSGT00550000074885.
    HOGENOMiHOG000020208.
    HOVERGENiHBG000634.
    InParanoidiP50554.
    KOiK13524.
    OMAiKLIQQPQ.
    OrthoDBiEOG7ZPNJW.
    PhylomeDBiP50554.
    TreeFamiTF105021.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    InterProiIPR004631. 4NH2But_aminotransferase_euk.
    IPR005814. Aminotrans_3.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PANTHERiPTHR11986. PTHR11986. 1 hit.
    PTHR11986:SF6. PTHR11986:SF6. 1 hit.
    PfamiPF00202. Aminotran_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
    PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P50554-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFLLTTRRL VCSSQKNLHL FTPGSRYISQ AAAKVDFEFD YDGPLMKTEV    50
    PGPRSQELMK QLNTIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI 100
    SSVPIGYNHP ALAKLVQQPQ NASTFINRPA LGILPPENFV DKLRESLMSV 150
    APKGMCQLIT MACGSCSNEN AFKTIFMWYR SKERGQRGFS KEELETCMVN 200
    QSPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS FDWPIAPFPR 250
    LKYPLEEFVT DNQQEEARCL EEVEDLIVKY RKKKRTVAGI IVEPIQSEGG 300
    DNHASDDFFR KLRDIARKHG CAFLVDEVQT GGGCTGKFWA HEHWGLDDPA 350
    DVMSFSKKMM TGGFFHKEEF RPSAPYRIFN TWLGDPSKNL LLAEVINIIK 400
    REDLLNNVAH AGKTLLTGLL DLQAQYPQFV SRVRGRGTFC SFDTPDEAIR 450
    NKLILIARNK GVVLGGCGDK SIRFRPTLVF RDHHAHLFLN IFSGILADFK 500
    Length:500
    Mass (Da):56,456
    Last modified:January 9, 2007 - v3
    Checksum:i1EA7180B72797B72
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31F → L in AAA70415. (PubMed:8133261)Curated
    Sequence conflicti25 – 251S → T in AAA70415. (PubMed:8133261)Curated
    Sequence conflicti36 – 361D → T in AAA70415. (PubMed:8133261)Curated
    Sequence conflicti135 – 17036PPENF…CSNEN → LQRTLWTSSGSPCSRWLPKA CVSSSRWPAGPAPMRM in AAA70415. (PubMed:8133261)CuratedAdd
    BLAST
    Sequence conflicti277 – 2771I → N in AAA70415. (PubMed:8133261)Curated
    Sequence conflicti425 – 4251Q → R in AAA70415. (PubMed:8133261)Curated
    Sequence conflicti447 – 4471E → K(PubMed:10447691)Curated
    Sequence conflicti447 – 4471E → K in BAA25570. (PubMed:10989446)Curated
    Sequence conflicti486 – 4861H → Q in AAA70415. (PubMed:8133261)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U29701 mRNA. Translation: AAA70415.1.
    D87839 mRNA. Translation: BAA25570.1.
    BC081787 mRNA. Translation: AAH81787.1.
    PIRiI56502.
    RefSeqiNP_112265.1. NM_031003.2.
    XP_006245822.1. XM_006245760.1.
    UniGeneiRn.10090.

    Genome annotation databases

    EnsembliENSRNOT00000003633; ENSRNOP00000003633; ENSRNOG00000002636.
    GeneIDi81632.
    KEGGirno:81632.
    UCSCiRGD:620948. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U29701 mRNA. Translation: AAA70415.1 .
    D87839 mRNA. Translation: BAA25570.1 .
    BC081787 mRNA. Translation: AAH81787.1 .
    PIRi I56502.
    RefSeqi NP_112265.1. NM_031003.2.
    XP_006245822.1. XM_006245760.1.
    UniGenei Rn.10090.

    3D structure databases

    ProteinModelPortali P50554.
    SMRi P50554. Positions 39-499.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000003633.

    Chemistry

    BindingDBi P50554.
    ChEMBLi CHEMBL3148.

    PTM databases

    PhosphoSitei P50554.

    2D gel databases

    World-2DPAGE 0004:P50554.

    Proteomic databases

    PaxDbi P50554.
    PRIDEi P50554.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000003633 ; ENSRNOP00000003633 ; ENSRNOG00000002636 .
    GeneIDi 81632.
    KEGGi rno:81632.
    UCSCi RGD:620948. rat.

    Organism-specific databases

    CTDi 18.
    RGDi 620948. Abat.

    Phylogenomic databases

    eggNOGi COG0160.
    GeneTreei ENSGT00550000074885.
    HOGENOMi HOG000020208.
    HOVERGENi HBG000634.
    InParanoidi P50554.
    KOi K13524.
    OMAi KLIQQPQ.
    OrthoDBi EOG7ZPNJW.
    PhylomeDBi P50554.
    TreeFami TF105021.

    Enzyme and pathway databases

    Reactomei REACT_196229. Degradation of GABA.
    SABIO-RK P50554.

    Miscellaneous databases

    NextBioi 615107.
    PROi P50554.

    Gene expression databases

    Genevestigatori P50554.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    InterProi IPR004631. 4NH2But_aminotransferase_euk.
    IPR005814. Aminotrans_3.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    PANTHERi PTHR11986. PTHR11986. 1 hit.
    PTHR11986:SF6. PTHR11986:SF6. 1 hit.
    Pfami PF00202. Aminotran_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR00699. GABAtrns_euk. 1 hit.
    PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A rat brain cDNA encodes enzymatically active GABA transaminase and provides a molecular probe for GABA-catabolizing cells."
      Medina-Kauwe L.K., Tillakaratne N.J., Wu J.Y., Tobin A.J.
      J. Neurochem. 62:1267-1275(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The mature size of rat 4-aminobutyrate aminotransferase is different in liver and brain."
      Kontani Y., Sakata S.F., Matsuda K., Ohyama T., Sano K., Tamaki N.
      Eur. J. Biochem. 264:218-222(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-54, PROTEOLYTIC PROCESSING.
      Strain: Sprague-Dawley.
      Tissue: Brain and Liver.
    3. "Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver."
      Tamaki N., Sakata S.F., Matsuda K.
      Methods Enzymol. 324:376-389(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Strain: Sprague-Dawley.
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    5. Lubec G., Afjehi-Sadat L.
      Submitted (NOV-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 253-268 AND 389-400, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Spinal cord.

    Entry informationi

    Entry nameiGABT_RAT
    AccessioniPrimary (citable) accession number: P50554
    Secondary accession number(s): O70539, Q66HM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3