P50554 (GABT_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-aminobutyrate aminotransferase, mitochondrial
EC=2.6.1.19

Alternative name(s):
(S)-3-amino-2-methylpropionate transaminase
EC=2.6.1.22
GABA aminotransferase
Short name=GABA-AT
Gamma-amino-N-butyrate transaminase
Short name=GABA transaminase
Short name=GABA-T
L-AIBAT

Cleaved into the following 2 chains:

1. 4-aminobutyrate aminotransferase, brain isoform
2. 4-aminobutyrate aminotransferase, liver isoform

Gene names

Name:	Abat
Synonyms:	Gabat

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
Catalytic activity	4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate. (S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer; disulfide-linked.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
PTM	Acetylation Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	behavioral response to cocaine Inferred from sequence or structural similarity. Source: UniProtKB copulation Inferred from mutant phenotype. Source: RGD gamma-aminobutyric acid catabolic process Inferred by curator Ref.2. Source: UniProtKB locomotory behavior Inferred from mutant phenotype. Source: RGD negative regulation of blood pressure Inferred from mutant phenotype. Source: RGD neurotransmitter catabolic process Inferred by curator Ref.2. Source: UniProtKB response to drug Inferred from direct assay. Source: RGD response to ethanol Inferred from direct assay. Source: RGD response to hypoxia Inferred from mutant phenotype. Source: RGD response to iron ion Inferred from direct assay. Source: RGD response to nicotine Inferred from mutant phenotype. Source: RGD
Cellular component	4-aminobutyrate transaminase complex Inferred from sequence or structural similarity. Source: UniProtKB mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell synaptosome Inferred from direct assay. Source: RGD
Molecular function	(S)-3-amino-2-methylpropionate transaminase activity Inferred from electronic annotation. Source: EC 4-aminobutyrate transaminase activity Inferred from direct assay Ref.2. Source: RGD protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB pyridoxal phosphate binding Inferred from sequence or structural similarity. Source: UniProtKB succinate-semialdehyde dehydrogenase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion
	Chain	28 – 500	473	4-aminobutyrate aminotransferase, brain isoform		PRO_0000001252
	Chain	35 – 500	466	4-aminobutyrate aminotransferase, liver isoform		PRO_0000001253
Amino acid modifications
	Modified residue	318	1	N6-acetyllysine By similarity
	Modified residue	357	1	N6-(pyridoxal phosphate)lysine
	Disulfide bond	163 ↔ 166		By similarity
	Disulfide bond	321		Interchain By similarity
Experimental info
	Sequence conflict	3	1	F → L in AAA70415. Ref.1
	Sequence conflict	25	1	S → T in AAA70415. Ref.1
	Sequence conflict	36	1	D → T in AAA70415. Ref.1
	Sequence conflict	135 – 170	36	PPENF…CSNEN → LQRTLWTSSGSPCSRWLPKA CVSSSRWPAGPAPMRM in AAA70415. Ref.1
	Sequence conflict	277	1	I → N in AAA70415. Ref.1
	Sequence conflict	425	1	Q → R in AAA70415. Ref.1
	Sequence conflict	447	1	E → K Ref.2
	Sequence conflict	447	1	E → K in BAA25570. Ref.3
	Sequence conflict	486	1	H → Q in AAA70415. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50554 [UniParc]. Last modified January 9, 2007. Version 3. Checksum: 1EA7180B72797B72	FASTA	500	56,456
	10 20 30 40 50 60 MAFLLTTRRL VCSSQKNLHL FTPGSRYISQ AAAKVDFEFD YDGPLMKTEV PGPRSQELMK 70 80 90 100 110 120 QLNTIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI SSVPIGYNHP ALAKLVQQPQ 130 140 150 160 170 180 NASTFINRPA LGILPPENFV DKLRESLMSV APKGMCQLIT MACGSCSNEN AFKTIFMWYR 190 200 210 220 230 240 SKERGQRGFS KEELETCMVN QSPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS 250 260 270 280 290 300 FDWPIAPFPR LKYPLEEFVT DNQQEEARCL EEVEDLIVKY RKKKRTVAGI IVEPIQSEGG 310 320 330 340 350 360 DNHASDDFFR KLRDIARKHG CAFLVDEVQT GGGCTGKFWA HEHWGLDDPA DVMSFSKKMM 370 380 390 400 410 420 TGGFFHKEEF RPSAPYRIFN TWLGDPSKNL LLAEVINIIK REDLLNNVAH AGKTLLTGLL 430 440 450 460 470 480 DLQAQYPQFV SRVRGRGTFC SFDTPDEAIR NKLILIARNK GVVLGGCGDK SIRFRPTLVF 490 500 RDHHAHLFLN IFSGILADFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A rat brain cDNA encodes enzymatically active GABA transaminase and provides a molecular probe for GABA-catabolizing cells." Medina-Kauwe L.K., Tillakaratne N.J., Wu J.Y., Tobin A.J. J. Neurochem. 62:1267-1275(1994) [PubMed: 8133261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The mature size of rat 4-aminobutyrate aminotransferase is different in liver and brain." Kontani Y., Sakata S.F., Matsuda K., Ohyama T., Sano K., Tamaki N. Eur. J. Biochem. 264:218-222(1999) [PubMed: 10447691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-54, PROTEOLYTIC PROCESSING. Strain: Sprague-Dawley. Tissue: Brain and Liver.
[3]	"Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver." Tamaki N., Sakata S.F., Matsuda K. Methods Enzymol. 324:376-389(2000) [PubMed: 10989446] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: Sprague-Dawley. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 253-268 AND 389-400, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29701 mRNA. Translation: AAA70415.1. D87839 mRNA. Translation: BAA25570.1. BC081787 mRNA. Translation: AAH81787.1.
IPI	IPI00199426.
PIR	I56502.
RefSeq	NP_112265.1. NM_031003.1.
UniGene	Rn.10090.
3D structure databases
ProteinModelPortal	P50554.
SMR	P50554. Positions 39-499.
ModBase	Search...
Protein-protein interaction databases
STRING	P50554.
PTM databases
PhosphoSite	P50554.
2D gel databases
World-2DPAGE	0004:P50554.
Proteomic databases
PRIDE	P50554.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000003633; ENSRNOP00000003633; ENSRNOG00000002636.
GeneID	81632.
KEGG	rno:81632.
NMPDR	fig|10116.3.peg.4435.
UCSC	NM_031003. rat.
Organism-specific databases
CTD	18.
RGD	620948. Abat.
Phylogenomic databases
eggNOG	roNOG08236.
GeneTree	ENSGT00550000074885.
HOVERGEN	HBG000634.
InParanoid	P50554.
OMA	ATGKFWA.
OrthoDB	EOG441QB6.
PhylomeDB	P50554.
Gene expression databases
ArrayExpress	P50554.
Genevestigator	P50554.
GermOnline	ENSRNOG00000002636. Rattus norvegicus.
Family and domain databases
InterPro	IPR004631. 4NH2But_aminotransferase_euk. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 2 hits.
KO	K13524.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF6. GABAtrns_euk. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00699. GABAtrns_euk. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615107.

Entry information

Entry name

GABT_RAT

Accession

Primary (citable) accession number: P50554
Secondary accession number(s): O70539, Q66HM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 9, 2007
Last modified:	November 16, 2011
This is version 99 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program